High-resolution microbiome analysis enabled by linking of 16S rRNA gene sequences with adjacent genomic contexts.
AuthorsKapustina Ž, Medžiune J, Alzbutas G, Rokaitis I, Matjošaitis K, Mackevicius G, Žeimyte S, Karpus L, Lubys A
JournalMicrob Genom
PubMed ID34473015
Sequence-based characterization of bacterial communities has long been a hostage of limitations of both 16S rRNA gene and whole metagenome sequencing. Neither approach is universally applicable, and the main efforts to resolve constraints have been devoted to improvement of computational prediction tools. Here, we present semi-targeted 16S rRNA sequencing (st16S-seq), ... More