Clean and Complete Protein Digestion with an Autolysis Resistant Trypsin for Peptide Mapping.
AuthorsMuriithi B,Ippoliti S,Finny A,Addepalli B,Lauber M
JournalJournal of proteome research
PubMed ID39392678
Peptide mapping requires cleavage of proteins in a predictable fashion so that target protein-specific peptides can be reliably identified and quantified. Trypsin, a commonly used protease in this process, can also undergo self-cleavage or autolysis, thereby reducing the effectivity and even cleavage specificity at lysine and arginine residues. Here, we ... More
Proximity-CLIP and Expedited Non-Radioactive Library Preparation of Small RNA Footprints for Next-Generation Sequencing.
AuthorsAnastasakis D,Benhalevy D,Hafner M
JournalCurrent protocols in molecular biology
PubMed ID32438484
During the course of their life cycle, most RNAs move between several cellular environments where they associate with different RNA binding proteins (RBPs). Reciprocally, a significant portion of RBPs reside in more than a single cellular compartment, where they can interact with discrete RNAs and even exert distinct biological roles. ... More