Proteomic and N-glycomic comparison of synthetic and bovine whey proteins and their effect on human gut microbiomes.
AuthorsBolino M,Duman H,Avcı İ,Kayili HM,Petereit J,Zundel C,Salih B,Karav S,Frese SA
JournalbioRxiv : the preprint server for biology
PubMed ID39345577
Advances in food production systems and customer acceptance have led to the commercial launch of dietary proteins produced via modern biotechnological approaches as alternatives to traditional agricultural sources. At the same time, a deeper understanding of how dietary components interact with the gut microbiome has highlighted the importance of understanding ... More
Optimized Workflow for Proteomics and Phosphoproteomics With Limited Tissue Samples.
AuthorsHu M,Wang Y
JournalCurrent protocols
PubMed ID38646944
Proteomics and phosphoproteomics play crucial roles in elucidating the dynamics of post-transcriptional processes. While experimental methods and workflows have been established in this field, a persistent challenge arises when dealing with small samples containing a limited amount of protein. This limitation can significantly impact the recovery of peptides and phosphopeptides. ... More
AuthorsVarnavides G, Madern M, Anrather D, Hartl N, Reiter W, Hartl M.
JournalJ Proteome Res
PubMed ID36006919
Robust, efficient, and reproducible protein extraction and sample processing is a key step for bottom-up proteomics analyses. While many sample preparation protocols for mass spectrometry have been described, selecting an appropriate method remains challenging since some protein classes may require specialized solubilization, precipitation, and digestion procedures. Here, we present a ... More