QuantStudio™ 1 实时荧光定量 PCR 系统，96-孔，0.2 mL，便携式计算机

引用和文献 (962)

Applied Biosystems™

QuantStudio™ 1 实时荧光定量 PCR 系统，96-孔，0.2 mL，便携式计算机

Applied Biosystems QuantStudio 1 实时荧光定量 PCR 系统质量与可靠性俱佳，是您的实用之选。这款经济实惠的仪器的基本功能适用于 qPCR 新用户或者预算有限的用户了解更多信息

货号	数量
A40427	1 System

货号 A40427

价格（CNY)

1 System

Applied Biosystems QuantStudio 1 实时荧光定量 PCR 系统质量与可靠性俱佳，是您的实用之选。这款经济实惠的仪器的基本功能适用于 qPCR 新用户或者预算有限的用户。尽管 QuantStudio 1 系统价格实惠，其仍提供了 QuantStudio 系列仪器的高质量、可靠性和高级用户体验。

下载 Quantstudio 1 设计与分析软件> | 版本说明

QuantStudio 1 系统的特点包括：
•触摸屏—8.5 英寸大型交互式触摸屏
•固定式模块—配备易开式手动抽屉的固定式 96 孔、0.2 mL 等温模块
•反应体积—10–100 μL
• 多通路能力—提供 3 种最常用的激发和发射滤光片：FAM、VIC 和 ROX
•方案—预优化的方案模板
•软件—支持通过网络浏览器或台式计算机进行分析的直观软件
•染料—经校准用于 FAM/SYBR Green/SYTO 9、VIC/HEX/TET/JOE 和 JUN/ROX/Texas Red 染料的 3 种滤光片
•灵敏度—检测到单重反应中小至 1.5 倍数量的差异
•尺寸（高 x 宽 x 深）和重量—40 x 27 x 50 cm，<26 kg

Thermo Fisher Connect
支持云技术服务的 Thermo Fisher Connect 为科学家存储、分析和共享数据提供了一个安全平台。从仪器上传数据或从任何具有网络连接的地方上传您自己的文件。Thermo Fisher Connect 中的 Applied Biosystems qPCR 分析模块是基于网络浏览器的软件工具，灵活、快速且易于使用。

易于使用
QuantStudio 1 实时荧光定量 PCR 系统结构紧凑、经工厂校准、以易用的包装形式提供，从而使安装快速简单以便立即使用。交互式触摸屏提供了现代化的可用性体验，简化的仪器软件/预优化的方案模板使任何经验水平的用户均可快速设置和启动运行。

设计和分析软件

选项 1：QuantStudio 设计和分析云端软件
• 支持使用 PC 或 MAC 计算机进行基于网络浏览器的系统配置
• 简化了软件流程，改善了可用性并缩短了响应时间
• 通过 Thermo Fisher Connect 可随时随地实现对您数据的安全访问
• 无需安装软件，无需许可证，无需版本更新

选项 2：QuantStudio 设计和分析桌面软件
• 支持对 Windows™ 7 和 Windows™ 10 OS 进行传统的同地协作计算机系统配置
• 简化了软件流程，改善了可用性并缩短了响应时间
• 无需购买许可证

值得信赖的结果
OptiFlex 技术实现了孔间和仪器间的数据准确性优化，该技术具有配备白光 LED 的 3 个偶联激发和发射滤光片通道

灵敏的数据分析
检测单重反应中低至 1.5 倍的靶标数量变化，并获得 10 个数量级倍数的线性动态范围。

高应用多功能性
可提供简单并优化的实验方案、可靠的测定和试剂以及直观的软件用于多种应用，包括基因表达、基因变异、基因调控等。

仅供科研使用。不可用于诊断程序。

深度（公制）50 cm

显示类型8.5英寸触摸屏

适用于（设备）QuantStudio™

产品规格96 孔板

高度（公制）40 cm

产品线Applied Biosystems™ QuantStudio

数量1 System

反应体积范围10 to 100 μL

运输条件经批准可在室温下或置于湿冰上运输

重量<26 kg

宽度（公制）27 cm

模块配置96孔

模块规格不可互换

Unit SizeEach

内容与储存

便携式计算机

常见问题解答 (FAQ)

What is the difference between continuous melt curves and step and hold melt curves on the Applied Biosystems Real-Time PCR Instruments?

Continuous melt curve: increases the temperature by the ramp increment (degrees C/sec) so that the temperature will change at a constant rate set in the protocol.
Step and Hold melt curve: increases the temperature by the ramp increment (degrees C) and then holds at that temperature for the time specified by the user.

The amount of data collected will depend on the filter(s) selected. The more filters you use, the longer the acquisition will take and the fewer data points will be collected.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Can I import a standard curve for my real-time PCR data into the Design & Analysis Software (v2.x)?

It is possible to import a standard curve into the Design & Analysis Software (v2.x) if you are analyzing results from one of the following Applied Biosystems real-time PCR instruments:
• 7900HT Fast Real-Time PCR System
• 7500/7500 Fast Real-Time PCR System
• StepOne/StepOne Plus Real-Time PCR System
• ViiA 7 Real Time PCR System
• QuantStudio 1 Real-Time PCR System
• QuantStudio 3 & 5 Real-Time PCR System
• QuantStudio 6 & 7 Flex Real-Time PCR System
• QuantStudio 6 & 7 Pro Real-Time PCR Systems
• QuantStudio 12K Flex Real-Time PCR system

Follow the instructions below to import a standard curve for analysis in the Design & Analysis Software (v2.x):
1. Open the data file in the Design & Analysis Software (v2.x).
2. Click on Actions.
3. Click on "Standard Curve Analysis Setting".
4. Select "External Standard Curves" and click on "Import".
5. Browse to the proper .csv file and click "Open".
6. Click "Apply".

Can I import a standard curve for my real-time PCR data into the QuantStudio Design & Analysis Software (v1.x)?

If you are analyzing results from the QuantStudio 1 Real-Time PCR System, QuantStudio 3 Real-Time PCR System, or QuantStudio 5 Real-Time PCR System, it is possible to import a standard curve into the QuantStudio Design & Analysis Software (v1.x) following the instructions below:
1. Open the data file in the QuantStudio Design & Analysis Software (v1.x).
2. Go to Analysis Settings.
3. Click on the "Standard Curve Settings" tab.
4. Select the option to "Use a standard curve imported from another experiment" and click on "Import".
5. Browse to and highlight the proper data file and click "Select".
6. Click Apply.

With the QuantStudio 1 instrument, why am I only able to export my run files to USB, whereas the cloud and network options are not available?

It is possible that the instrument is not connected to the computer and is not linked with the Thermo Fisher Cloud. Please see page 38 of the QuantStudio 1 Real-Time PCR System User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017853_QS1%20UG.pdf) for instructions to link an instrument profile with the Thermo Fisher Cloud. Although default installation instructions recommend using an Ethernet cable, it can also be done wirelessly using a High Power USB WiFi Module, Cat. No. A26774 (https://www.thermofisher.com/order/catalog/product/A26774).

If you experience connection issues between the instrument and computer, it might be useful setting a static IP address on both the instrument and computer. On the computer, you can use online help, such as this one (https://kb.netgear.com/27476/How-do-I-set-a-static-IP-address-in-Windows) to set the static IP address. Here are the details for the settings:

- Computer IP: 10.0.0.2
- Instrument IP: 10.0.0.3
- Subnet: 255.255.255.0
- DNS1: 8.8.8.8
- DNS2: 8.8.4.4
- Default gateway: 10.0.0.1

On the instrument, the IP address can be found under Settings and About Instrument. If you see an IP address but are still having network issues, try to PING the IP Address in a command window of the computer:

- Select the windows start button and select the RUN command.
- Open the Run command and type in CMD.
- This will open the Command window.
- Type the word "PING", space, followed by the IP address and press enter.
- You should see 4 packets of data sent, 4 received, and 0 lost.

If you PING the IP Address successfully (4 sent, 4 received, 0 lost), but still cannot connect, it means that one of the needed ports is still not open. Please contact your IT department and review the instrument checklist.
If you PING the IP address unsuccessfully, please send us the serial number of the instrument so that we can contact your local engineer.

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

Where can I find the software and newest firmware for the QuantStudio 1 instrument?

In an effort to reduce environmental impact, we no longer supply the software on the CD. Please download it from here (https://www.thermofisher.com/uk/en/home/global/forms/life-science/quantstudio-software-download.html).

Find additional tips, troubleshooting help, and resources within our Real-Time PCR and Digital PCR Instruments Support Center.

View all QuantStudio™ 1 实时荧光定量 PCR 系统，96-孔，0.2 mL，便携式计算机 FAQs

引用和文献 (962)

引用和文献

Abstract

Comparable type I interferon score determination from PAXgene and Tempus whole blood RNA collection and isolation systems.

Authors:Lamot L,Niemietz I,Brown KL

Journal:BMC research notes

PubMed ID:31416482

OBJECTIVE: Type I interferons (IFN) have important roles in many immune-mediated inflammatory diseases (IMIDs) and are a relatively new therapeutic target. Direct detection of type I IFNs has proved challenging, thus their presence is often inferred from the expression of interferon-stimulated genes (ISGs) and calculation of an interferon score (IS). ... More

Efficient gene editing of human long-term hematopoietic stem cells validated by clonal tracking.

Authors:Ferrari S,Jacob A,Beretta S,Unali G,Albano L,Vavassori V,Cittaro D,Lazarevic D,Brombin C,Cugnata F,Kajaste-Rudnitski A,Merelli I,Genovese P,Naldini L

Journal:Nature biotechnology

PubMed ID:32601433

Targeted gene editing in hematopoietic stem cells (HSCs) is a promising treatment for several diseases. However, the limited efficiency of homology-directed repair (HDR) in HSCs and the unknown impact of the procedure on clonal composition and dynamics upon transplantation have hampered clinical translation. Here, we apply a barcoding strategy to ... More

Tamoxifen induction of Cre recombinase does not cause long-lasting or sexually divergent responses in the CNS epigenome or transcriptome: implications for the design of aging studies.

Authors:Chucair-Elliott AJ,Ocanas SR,Stanford DR,Hadad N,Wronowski B,Otalora L,Stout MB,Freeman WM

Journal:GeroScience

PubMed ID:31493147

The systemic delivery of tamoxifen (Tam) to activate inducible CreERT2-loxP transgenic mouse systems is now widely used in neuroscience studies. This critical technological advancement allows temporal control of DNA-cre recombination, avoidance of embryonically lethal phenotypes, and minimization of residual cell labeling encountered in constitutively active drivers. Despite its advantages, the ... More

CD4(+) CCR6(+) T cells, but not γδ T cells, are important for the IL-23R-dependent progression of antigen-induced inflammatory arthritis in mice.

Authors:Razawy W,Asmawidjaja PS,Mus AM,Salioska N,Davelaar N,Kops N,Oukka M,Alves CH,Lubberts E

Journal:European journal of immunology

PubMed ID:31778214

ILâ23 plays an important role in the development of arthritis and the ILâ23 receptor (ILâ23R) is expressed on different types of TÂ cells. However, it is not fully clear which ILâ23R(+) TÂ cells are critical in driving TÂ cellâmediated synovitis. We demonstrate, using knockâin ILâ23RâGFP reporter (ILâ23R(GFP/+)) mice, that CD4(+)CCR6(+) TÂ cells and Î³Î´ ... More

Inactivation of Pepper Mild Mottle Virus in Water by Cold Atmospheric Plasma.

Authors:Filipić A,Dobnik D,Tušek Žnidarič M,Žegura B,Štern A,Primc G,Mozetič M,Ravnikar M,Žel J,Gutierrez Aguirre I

Journal:Frontiers in microbiology

PubMed ID:33584622

Water scarcity is one of the greatest threats for human survival and quality of life, and this is increasingly contributing to the risk of human, animal and plant infections due to waterborne viruses. Viruses are transmitted through polluted water, where they can survive and cause infections even at low concentrations. ... More

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