Comparable type I interferon score determination from PAXgene and Tempus whole blood RNA collection and isolation systems.
AuthorsLamot L,Niemietz I,Brown KL
JournalBMC research notes
PubMed ID31416482
OBJECTIVE: Type I interferons (IFN) have important roles in many immune-mediated inflammatory diseases (IMIDs) and are a relatively new therapeutic target. Direct detection of type I IFNs has proved challenging, thus their presence is often inferred from the expression of interferon-stimulated genes (ISGs) and calculation of an interferon score (IS). ... More
Efficient gene editing of human long-term hematopoietic stem cells validated by clonal tracking.
Targeted gene editing in hematopoietic stem cells (HSCs) is a promising treatment for several diseases. However, the limited efficiency of homology-directed repair (HDR) in HSCs and the unknown impact of the procedure on clonal composition and dynamics upon transplantation have hampered clinical translation. Here, we apply a barcoding strategy to ... More
Tamoxifen induction of Cre recombinase does not cause long-lasting or sexually divergent responses in the CNS epigenome or transcriptome: implications for the design of aging studies.
The systemic delivery of tamoxifen (Tam) to activate inducible CreERT2-loxP transgenic mouse systems is now widely used in neuroscience studies. This critical technological advancement allows temporal control of DNA-cre recombination, avoidance of embryonically lethal phenotypes, and minimization of residual cell labeling encountered in constitutively active drivers. Despite its advantages, the ... More
CD4(+) CCR6(+) T cells, but not γδ T cells, are important for the IL-23R-dependent progression of antigen-induced inflammatory arthritis in mice.
AuthorsRazawy W,Asmawidjaja PS,Mus AM,Salioska N,Davelaar N,Kops N,Oukka M,Alves CH,Lubberts E
JournalEuropean journal of immunology
PubMed ID31778214
ILâ23 plays an important role in the development of arthritis and the ILâ23 receptor (ILâ23R) is expressed on different types of T cells. However, it is not fully clear which ILâ23R(+) T cells are critical in driving T cellâmediated synovitis. We demonstrate, using knockâin ILâ23RâGFP reporter (ILâ23R(GFP/+)) mice, that CD4(+)CCR6(+) T cells and γδ ... More
Inactivation of Pepper Mild Mottle Virus in Water by Cold Atmospheric Plasma.
Water scarcity is one of the greatest threats for human survival and quality of life, and this is increasingly contributing to the risk of human, animal and plant infections due to waterborne viruses. Viruses are transmitted through polluted water, where they can survive and cause infections even at low concentrations. ... More
The Identification of Plasma Exosomal miR-423-3p as a Potential Predictive Biomarker for Prostate Cancer Castration-Resistance Development by Plasma Exosomal miRNA Sequencing.
JournalFrontiers in cell and developmental biology
PubMed ID33490068
Castration-resistant prostate cancer (CRPC) is the major cause of death from prostate cancer. Biomarkers to improve early detection and prediction of CRPC especially using non-invasive liquid biopsies could improve outcomes. Therefore, we investigated the plasma exosomal miRNAs associated with CRPC and their potential for development into non-invasive early detection biomarkers ... More
Transcriptional regulatory dynamics drive coordinated metabolic and neural response to social challenge in mice.
Agonistic encounters are powerful effectors of future behavior, and the ability to learn from this type of social challenge is an essential adaptive trait. We recently identified a conserved transcriptional program defining the response to social challenge across animal species, highly enriched in transcription factor (TF), energy metabolism, and developmental ... More
Tissue-specific transcriptome analyses reveal candidate genes for stilbene, flavonoid and anthraquinone biosynthesis in the medicinal plant Polygonum cuspidatum.
AuthorsWang X,Hu H,Wu Z,Fan H,Wang G,Chai T,Wang H
JournalBMC genomics
PubMed ID34000984
BACKGROUND: Polygonum cuspidatum Sieb. et Zucc. is a well-known medicinal plant whose pharmacological effects derive mainly from its stilbenes, anthraquinones, and flavonoids. These compounds accumulate differentially in the root, stem, and leaf; however, the molecular basis of such tissue-specific accumulation remains poorly understood. Because tissue-specific accumulation of compounds is usually ... More
Beta cell adaptation to pregnancy requires prolactin action on both beta and non-beta cells.
AuthorsShrivastava V,Lee M,Lee D,Pretorius M,Radford B,Makkar G,Huang C
JournalScientific reports
PubMed ID33990661
Pancreatic islets adapt to insulin resistance of pregnancy by up regulating β-cell mass and increasing insulin secretion. Previously, using a transgenic mouse with global, heterozygous deletion of prolactin receptor (Prlr(+/â)), we found Prlr signaling is important for this adaptation. However, since Prlr is expressed in tissues outside of islets as ... More
Intraductal Transplantation Models of Human Pancreatic Ductal Adenocarcinoma Reveal Progressive Transition of Molecular Subtypes.
Pancreatic ductal adenocarcinoma (PDAC) is the most lethal common malignancy, with little improvement in patient outcomes over the past decades. Recently, subtypes of pancreatic cancer with different prognoses have been elaborated, however the inability to model these subtypes has precluded mechanistic investigation of their origins. Here, we present a xenotransplantation ... More
Obesity Prevents S-Adenosylmethionine-Mediated Improvements in Age-Related Peripheral and Hippocampal Outcomes.
AuthorsVander Velden JW,Osborne DM
JournalNutrients
PubMed ID33917279
Background: Age predisposes individuals to a myriad of disorders involving inflammation; this includes stress-related neuropsychiatric disorders such as depression and anxiety, and neurodegenerative diseases. Obesity can further exacerbate these effects in the brain. We investigated whether an inexpensive dietary supplement, s-adenosylmethionine (SAMe), could improve age- and/or obesity-related inflammatory and affective ... More
Impact of continuous predator threat on telomere dynamics in parent and nestling pied flycatchers.
AuthorsKärkkäinen T,Teerikorpi P,Panda B,Helle S,Stier A,Laaksonen T
JournalOecologia
PubMed ID31612326
In addition to direct mortality, predators can have indirect effects on prey populations by affecting prey behaviour or physiology. For example, predator presence can increase stress hormone levels, which can have physiological costs. Stress exposure accelerates the shortening of telomeres (i.e. the protective caps of chromosomes) and shorter telomeres have ... More
Using viral load and epidemic dynamics to optimize pooled testing in resource constrained settings.
JournalmedRxiv : the preprint server for health sciences
PubMed ID32511487
Extensive virological testing is central to SARS-CoV-2 containment, but many settings face severe limitations on testing. Group testing offers a way to increase throughput by testing pools of combined samples; however, most proposed designs have not yet addressed key concerns over sensitivity loss and implementation feasibility. Here, we combine a ... More
A pan-inflammatory microRNA-cluster is associated with orbital non-Hodgkin lymphoma and idiopathic orbital inflammation.
AuthorsLaban KG,Kalmann R,Bekker CPJ,Hiddingh S,van der Veen RLP,Eenhorst CAE,Genders SW,Mourits MP,Verhagen FH,Leijten EFA,Haitjema S,de Groot MCH,Radstake TRDJ,de Boer JH,Kuiper JJW
JournalEuropean journal of immunology
PubMed ID31713839
NonâHodgkin orbital lymphoma (NHOL) and idiopathic orbital inflammation (IOI) are common orbital conditions with largely unknown pathophysiology that can be difficult to diagnose. In this study we aim to identify serum miRNAs associated with NHOL and IOI. We performed OpenArray (®) miRNA profiling in 33 patients and controls. Differentially expressed ... More
Inactivating histone deacetylase HDA promotes longevity by mobilizing trehalose metabolism.
Histone acetylations are important epigenetic markers for transcriptional activation in response to metabolic changes and various stresses. Using the high-throughput SEquencing-Based Yeast replicative Lifespan screen method and the yeast knockout collection, we demonstrate that the HDA complex, a class-II histone deacetylase (HDAC), regulates aging through its target of acetylated H3K18 ... More
Schizochytrium sp. (T18) Oil as a Fish Oil Replacement in Diets for Juvenile Rainbow Trout (Oncorhynchus mykiss): Effects on Growth Performance, Tissue Fatty Acid Content, and Lipid-Related Transcript Expression.
AuthorsOsmond ATY,Arts MT,Hall JR,Rise ML,Bazinet RP,Armenta RE,Colombo SM
JournalAnimals : an open access journal from MDPI
PubMed ID33924273
SIMPLE SUMMARY: One of the main concerns in aquaculture is the overreliance on wild-sourced fish oil as the main source of omega-3 fatty acids in diets for farmed fish. Microbes, such as Schizochytrium, naturally produce high levels of omega-3 fatty acids that could potentially replace fish oil in aquaculture feeds. ... More
Stromal cells maintain immune cell homeostasis in adipose tissue via production of interleukin-33.
AuthorsMahlakõiv T,Flamar AL,Johnston LK,Moriyama S,Putzel GG,Bryce PJ,Artis D
JournalScience immunology
PubMed ID31053655
Obesity is driven by chronic low-grade inflammation resulting from dysregulated immune cell accumulation and function in white adipose tissue (WAT). Interleukin-33 (IL-33) is a key cytokine that controls innate and adaptive immune cell activity and immune homeostasis in WAT, although the sources of IL-33 have remained controversial. Here, we show ... More
Electrostatic interactions modulate the differential aggregation propensities of IgG1 and IgG4P antibodies and inform charged residue substitutions for improved developability.
Native state aggregation is an important concern in the development of therapeutic antibodies. Enhanced knowledge of mAb native state aggregation mechanisms would permit sequence-based selection and design of therapeutic mAbs with improved developability. We investigated how electrostatic interactions affect the native state aggregation of seven human IgG1 and IgG4P mAb ... More
Changes in systemic and subcutaneous adipose tissue inflammation and oxidative stress in response to exercise training in obese black African women.
Mycobacterium tuberculosis (Mtb) infection induces pulmonary expression of the heme-degrading enzyme heme oxygenase-1 (HO-1). We have previously shown that pharmacological inhibition of HO-1 activity in experimental tuberculosis results in decreased bacterial loads and unexpectedly that this outcome depends on the presence of T lymphocytes. Here we extend these findings by ... More
The PAX-SIX-EYA-DACH network modulates GATA-FOG function in fly hematopoiesis and human erythropoiesis.
The GATA and PAX-SIX-EYA-DACH transcriptional networks (PSEDNs) are essential for proper development across taxa. Here, we demonstrate novel PSEDN roles in vivo in Drosophila hematopoiesis and in human erythropoiesis in vitro. Using Drosophila genetics, we show that PSEDN members function with GATA to block lamellocyte differentiation and maintain the prohemocyte ... More
Comparison of methodologies for the detection of BRAF mutations in bone marrow trephine specimens.
AuthorsCardus B,Colling R,Hamblin A,Soilleux E
JournalJournal of clinical pathology
PubMed ID30872385
Distinctive in-planta acclimation responses to basal growth and acute heat stress were induced in Arabidopsis by cattle manure biochar.
AuthorsKumar A,Friedman H,Tsechansky L,Graber ER
JournalScientific reports
PubMed ID33972570
In-planta mechanisms of biochar (BC)-mediated improved growth were evaluated by examining oxidative stress, metabolic, and hormonal changes of Arabidopsis wild-type plants under basal or acute heat stress (âHS/â+âHS) conditions with or without BC (+âBC/âBC). The oxidative stress was evaluated by using Arabidopsis expressing redox-sensitive green fluorescent protein in the plastids ... More
Oncogene-dependent function of BRG1 in hepatocarcinogenesis.
Hepatocellular carcinoma (HCC) is the major type of primary liver cancer. Genomic studies have revealed that HCC is a heterogeneous disease with multiple subtypes. BRG1, encoded by the SMARCA4 gene, is a key component of SWI/SNF chromatin-remodeling complexes. Based on TCGA studies, somatic mutations of SMARCA4 occur in ~3% of ... More
Therapeutic effects of higenamine combined with [6]-gingerol on chronic heart failure induced by doxorubicin via ameliorating mitochondrial function.
Higenamine (HG) is a natural benzylisoquinoline alkaloid isolated from Aconitum with positive inotropic and chronotropic effects. This study aimed to investigate the possible cardioprotective effects of HG combined with [6]âgingerol (HG/[6]âGR) against DOXâinduced chronic heart failure (CHF) by comprehensive approaches. DOXâinduced cardiotoxicity model in rats and H9c2 cells was established. ... More
The associations of hostility and defensiveness with telomere length are influenced by sex and health status.
AuthorsStarnino L,Dupuis G,Busque L,Bourgoin V,Dubé MP,Busseuil D,D'Antono B
JournalBiology of sex differences
PubMed ID33397445
BACKGROUND: Shorter telomere length (TL) may indicate premature cellular aging and increased risk for disease. While there is substantial evidence for shorter TL in individuals suffering from psychiatric disorders, data is scarce on maladaptive personality traits related to coronary artery disease (CAD). The purpose of this study was to evaluate ... More
Effects of high glucose conditions on the expansion and differentiation capabilities of mesenchymal stromal cells derived from rat endosteal niche.
AuthorsAl-Qarakhli AMA,Yusop N,Waddington RJ,Moseley R
JournalBMC molecular and cell biology
PubMed ID31752674
BACKGROUND: Mesenchymal stromal cells in the endosteal niche lining compact bone (CB-MSCs) represent a heterogeneous population, all of which contribute to bone repair and remodelling. Hyperglycaemia associated with type 2 diabetes mellitus (T2DM) can delay and impair the bone healing process. Therefore, this study investigated the influences of high (25âmM) ... More
Human Synovia Contains Trefoil Factor Family (TFF) Peptides 1-3 Although Synovial Membrane Only Produces TFF3: Implications in Osteoarthritis and Rheumatoid Arthritis.
JournalInternational journal of molecular sciences
PubMed ID31817054
Objective: Trefoil factor family peptide 3 (TFF3) has been shown to support catabolic functions in cases of osteoarthritis (OA). As in joint physiology and diseases such as OA, the synovial membrane (SM) of the joint capsule also plays a central role. We analyze the ability of SM to produce TFF ... More
Neurocognitive outcomes in indonesians living with HIV are influenced by polymorphisms in the gene encoding purinergic P2X receptor 7.
AuthorsGaff J,Estiasari R,Diafiri D,Halstrom S,Kamerman P,Price P
JournalBrain, behavior, & immunity - health
PubMed ID34589739
The advent of effective antiretroviral therapy (ART) has decreased the prevalence and severity of HIV-associated neurocognitive disorders (HAND), but milder forms of HAND remain despite optimal treatment. Neuronal injury and loss due to inflammation may mediate HAND. P2X7R encodes purinergic P2X receptor 7 which influences neuroinflammatory pathways and carries polymorphisms ... More
Generation, analysis, and transformation of macro-chloroplast Potato (Solanum tuberosum) lines for chloroplast biotechnology.
Chloroplast biotechnology is a route for novel crop metabolic engineering. The potential bio-confinement of transgenes, the high protein expression and the possibility to organize genes into operons represent considerable advantages that make chloroplasts valuable targets in agricultural biotechnology. In the last 3 decades, chloroplast genomes from a few economically important ... More
The BIRC Family Genes Expression in Patients with Triple Negative Breast Cancer.
AuthorsMakuch-Kocka A,Kocki J,Brzozowska A,Bogucki J,Kołodziej P,Płachno BJ,Bogucka-Kocka A
JournalInternational journal of molecular sciences
PubMed ID33673050
The BIRC (baculoviral IAP repeat-containing; BIRC) family genes encode for Inhibitor of Apoptosis (IAP) proteins. The dysregulation of the expression levels of the genes in question in cancer tissue as compared to normal tissue suggests that the apoptosis process in cancer cells was disturbed, which may be associated with the ... More
The application of a biostimulant based on tannins affects root architecture and improves tolerance to salinity in tomato plants.
AuthorsCampobenedetto C,Mannino G,Beekwilder J,Contartese V,Karlova R,Bertea CM
JournalScientific reports
PubMed ID33432010
Roots have important roles for plants to withstand adverse environmental conditions, including salt stress. Biostimulant application was shown to enhance plant resilience towards abiotic stresses. Here, we studied the effect of a tannin-based biostimulant on tomato (Solanum lycopersicum L.) grown under salt stress conditions. We investigated the related changes at ... More
Ultra-deep sequencing reveals dramatic alteration of organellar genomes in Physcomitrella patens due to biased asymmetric recombination.
AuthorsOdahara M,Nakamura K,Sekine Y,Oshima T
JournalCommunications biology
PubMed ID34045660
Destabilization of organelle genomes causes organelle dysfunction that appears as abnormal growth in plants and diseases in human. In plants, loss of the bacterial-type homologous recombination repair (HRR) factors RECA and RECG induces organelle genome instability. In this study, we show the landscape of organelle genome instability in Physcomitrella patens ... More
Long non-coding RNAs as novel therapeutic targets in juvenile myelomonocytic leukemia.
Juvenile myelomonocytic leukemia (JMML) treatment primarily relies on hematopoietic stem cell transplantation and results in long-term overall survival of 50â60%, demonstrating a need to develop novel treatments. Dysregulation of the non-coding RNA transcriptome has been demonstrated before in this rare and unique disorder of early childhood. In this study, we ... More
α-Ketoglutarate links p53 to cell fate during tumour suppression.
The tumor suppressor TP53 is mutated in the majority of human cancers and over 70% of pancreatic ductal adenocarcinoma (PDAC)(1,2). Wild-type p53 accumulates in response to cellular stress and regulates gene expression to alter cell fate and prevent tumor development(2). Wild-type p53 also modulates cellular metabolic pathways(3), though p53-dependent metabolic ... More
Dual inhibition of endothelial miR-92a-3p and miR-489-3p reduces renal injury-associated atherosclerosis.
BACKGROUND AND AIMS: Cardiovascular disease (CVD) is the leading cause of death in chronic kidney disease (CKD) patients, however, the underlying mechanisms that link CKD and CVD are not fully understood and limited treatment options exist in this high-risk population. microRNAs (miRNA) are critical regulators of gene expression for many ... More
Comparison of Bacterial DNA Profiles in Mid-Trimester Amniotic Fluid Samples From Preterm and Term Deliveries.
AuthorsStinson L,Hallingström M,Barman M,Viklund F,Keelan J,Kacerovsky M,Payne M,Jacobsson B
JournalFrontiers in microbiology
PubMed ID32265868
Infection and inflammation are well recognized causes of spontaneous preterm delivery (PTD) (<37 gestational weeks) and adverse infant outcomes. To date, there has been very little investigation into bacterial communities in amniotic fluid using next generation sequencing technology. In particular, it is important to characterize amniotic fluid bacterial profiles in ... More
The Effect of Antimicrobial Treatment upon the Gill Bacteriome of Atlantic Salmon (Salmo salar L.) and Progression of Amoebic Gill Disease (AGD) In Vivo.
Branchial surfaces of finfish species contain a microbial layer rich in commensal bacteria which can provide protection through competitive colonization and production of antimicrobial products. Upon disturbance or compromise, pathogenic microbiota may opportunistically infiltrate this protective barrier and initiate disease. Amoebic gill disease (AGD) is a globally significant health condition ... More
Identification the ferroptosis-related gene signature in patients with esophageal adenocarcinoma.
BACKGROUND: Ferroptosis is a recently recognized non-apoptotic cell death that is distinct from the apoptosis, necroptosis and pyroptosis. Considerable studies have demonstrated ferroptosis is involved in the biological process of various cancers. However, the role of ferroptosis in esophageal adenocarcinoma (EAC) remains unclear. This study aims to explore the ferroptosis-related ... More
Identification and evaluation of reference genes for quantitative real-time PCR analysis in Polygonum cuspidatum based on transcriptome data.
AuthorsWang X,Wu Z,Bao W,Hu H,Chen M,Chai T,Wang H
JournalBMC plant biology
PubMed ID31726985
BACKGROUND: Polygonum cuspidatum of the Polygonaceae family is a traditional medicinal plant with many bioactive compounds that play important roles in human health and stress responses. Research has attempted to identify biosynthesis genes and metabolic pathways in this species, and quantitative real-time PCR (RT-qPCR) has commonly been used to detect ... More
Antigen-responsive CD4+ T cell clones contribute to the HIV-1 latent reservoir.
Antiretroviral therapy suppresses but does not cure HIV-1 infection due to the existence of a long-lived reservoir of latently infected cells. The reservoir has an estimated half-life of 44 mo and is largely composed of clones of infected CD4(+) T cells. The long half-life appears to result in part from ... More
Epigenetic silencing of MEIS2 in prostate cancer recurrence.
BACKGROUND: Current diagnostic and prognostic tools for prostate cancer (PC) are suboptimal, resulting in overdiagnosis and overtreatment of clinically insignificant tumors. Thus, to improve the management of PC, novel biomarkers are urgently needed. RESULTS: In this study, we integrated genome-wide methylome (Illumina 450K DNA methylation array (450K)) and RNA sequencing ... More
Berberine Attenuates Chronic Atrophic Gastritis Induced by MNNG and Its Potential Mechanism.
AuthorsTong Y,Liu L,Wang R,Yang T,Wen J,Wei S,Jing M,Zou W,Zhao Y
JournalFrontiers in pharmacology
PubMed ID33841162
The purpose of this study was to investigate the therapeutic effect of berberine (BBR) on MNNG-induced chronic atrophic gastritis (CAG) and the possible mechanism of BBR through TGF-β1/PI3K signal pathway. GES-1 were pretreated with MNNG for 2 h before BBR treatment in all procedures. Cell viability was quantified by cell counting ... More
Evolution and Clinical Impact of EGFR Mutations in Circulating Free DNA in the BELIEF Trial.
Glutamine synthetase (GS) generates glutamine from glutamate and controls the release of inflammatory mediators. In macrophages, GS activity, driven by IL10, associates to the acquisition of M2âlike functions. Conditional deletion of GS in macrophages inhibits metastasis by boosting the formation of antiâtumor, M1âlike, tumorâassociated macrophages (TAMs). From this basis, we ... More
Receptor-Driven ERK Pulses Reconfigure MAPK Signaling and Enable Persistence of Drug-Adapted BRAF-Mutant Melanoma Cells.
Targeted inhibition of oncogenic pathways can be highly effective in halting the rapid growth of tumors but often leads to the emergence of slowly dividing persister cells, which constitute a reservoir for the selection of drug-resistant clones. In BRAF(V600E) melanomas, RAF and MEK inhibitors efficiently block oncogenic signaling, but persister ... More
Molecular analysis of the blood meals and bacterial communities of bed bugs (Cimex lectularius L.) to assess interactions with alternative hosts.
AuthorsPotts R,Peta V,Agany DDM,Gnimpieba EZ,Cooper R,Wang C,Pietri JE
JournalParasitology research
PubMed ID33559752
Common bed bugs (Cimex lectularius L.) are hematophagous pests present in urban environments across the globe. It is widely established that they have a strong host preference for humans. However, there are records of C. lectularius feeding upon a range of mammalian and avian hosts, including rodents, in the field. ... More
Identification and Functional Study of Chitin Metabolism and Detoxification-Related Genes in Glyphodes pyloalis Walker (Lepidoptera: Pyralidae) Based on Transcriptome Analysis.
AuthorsShao ZM,Li YJ,Zhang XR,Chu J,Ma JH,Liu ZX,Wang J,Sheng S,Wu FA
JournalInternational journal of molecular sciences
PubMed ID32164390
Glyphodes pyloalis Walker (Lepidoptera: Pyralididae) is a serious pest in the sericulture industry, which has caused damage and losses in recent years. With the widespread use of insecticides, the insecticide resistance of G. pyloalis has becomes increasingly apparent. In order to find other effective methods to control G. pyloalis, this ... More
Type I IFNs facilitate innate immune control of the opportunistic bacteria Burkholderia cenocepacia in the macrophage cytosol.
The mammalian immune system is constantly challenged by signals from both pathogenic and non-pathogenic microbes. Many of these non-pathogenic microbes have pathogenic potential if the immune system is compromised. The importance of type I interferons (IFNs) in orchestrating innate immune responses to pathogenic microbes has become clear in recent years. ... More
Suppression of TGF-β1 signaling by Matrigel via FAK signaling in cultured human trabecular meshwork cells.
AuthorsZhang Y,Tseng SCG,Zhu YT
JournalScientific reports
PubMed ID33795740
The trabecular meshwork (TM) is composed of TM cells and beams of the extracellular matrix, together contributing to aqueous humor (AH) outflow resistance. Herein, we validated that our culture system on 2D Matrigel expressed putative TM markers and myocilin, of which the latter was upregulated by dexamethasone. Continuous passage of ... More
Vector Competence for DENV-2 Among Aedes albopictus (Diptera: Culicidae) Populations in China.
AuthorsWei Y,Wang J,Wei YH,Song Z,Hu K,Chen Y,Zhou G,Zhong D,Zheng X
JournalFrontiers in cellular and infection microbiology
PubMed ID33834007
Aedes albopictus is a vector of over 20 arboviruses that has spread throughout the world, mainly in the second half of the twentieth century. Approximately 50â100 million people are infected with dengue virus (DENV) transmitted by Aedes mosquitoes each year, leading to heavy economic burdens for both governments and individuals, ... More
Pharmacological rescue in patient iPSC and mouse models with a rare DISC1 mutation.
We previously identified a causal link between a rare patient mutation in DISC1 (disrupted-in-schizophrenia 1) and synaptic deficits in cortical neurons differentiated from isogenic patient-derived induced pluripotent stem cells (iPSCs). Here we find that transcripts related to phosphodiesterase 4 (PDE4) signaling are significantly elevated in human cortical neurons differentiated from ... More
Screening of Reference Genes for RT-qPCR in Chicken Adipose Tissue and Adipocytes.
AuthorsNa W,Wang Y,Gong P,Zhang X,Zhang K,Zhang H,Wang N,Li H
JournalFrontiers in physiology
PubMed ID34054585
Reverse transcription quantitative real-time PCR is the most commonly used method to detect gene expression levels. In experiments, it is often necessary to correct and standardize the expression level of target genes with reference genes. Therefore, it is very important to select stable reference genes to obtain accurate quantitative results. ... More
Detection of Reproducible Major Effect QTL for Petal Traits in Garden Roses.
AuthorsSchulz D,Linde M,Debener T
JournalPlants (Basel, Switzerland)
PubMed ID33946713
The detection of QTL by association genetics depends on the genetic architecture of the trait under study, the size and structure of the investigated population and the availability of phenotypic and marker data of sufficient quality and quantity. In roses, we previously demonstrated that major QTL could already be detected ... More
Association of differentially expressed R-gene candidates with leaf spot resistance in peanut (Arachis hypogaea L.).
AuthorsDang PM,Lamb MC,Chen CY
JournalMolecular biology reports
PubMed ID33403558
Early leaf spot (ELS) and late leaf spot (LLS) are major fungal diseases of peanut that can severely reduce yield and quality. Development of acceptable genetic resistance has been difficult due to a strong environmental component and many major and minor QTLs. Resistance genes (R-genes) are an important component of ... More
Dietary intake of bioactive ingredients impacts liver and adipose tissue transcriptomes in a porcine model of prepubertal early obesity.
Global prevalence of obesity has increased to epidemic proportions over the past 40 years, with childhood obesity reaching alarming rates. In this study, we determined changes in liver and adipose tissue transcriptomes of a porcine model for prepubertal early obesity induced by a high-calorie diet and supplemented with bioactive ingredients. ... More
mRNA localization mediates maturation of cytoplasmic cilia in Drosophila spermatogenesis.
AuthorsFingerhut JM,Yamashita YM
JournalThe Journal of cell biology
PubMed ID32706373
Cytoplasmic cilia, a specialized type of cilia in which the axoneme resides within the cytoplasm rather than within the ciliary compartment, are proposed to allow for the efficient assembly of very long cilia. Despite being found diversely in male gametes (e.g., Plasmodium falciparum microgametocytes and human and Drosophila melanogaster sperm), ... More
Influence of Physicochemical Characteristics and Stability of Gold and Silver Nanoparticles on Biological Effects and Translocation across an Intestinal Barrier-A Case Study from In Vitro to In Silico.
A better understanding of their interaction with cell-based tissue is a fundamental prerequisite towards the safe production and application of engineered nanomaterials. Quantitative experimental data on the correlation between physicochemical characteristics and the interaction and transport of engineered nanomaterials across biological barriers, in particular, is still scarce, thus hampering the ... More
A prion-like domain in ELF3 functions as a thermosensor in Arabidopsis.
Group 2 innate lymphoid cells (ILC2s) regulate immunity, inflammation and tissue homeostasis. Two distinct subsets of ILC2s have been described: steady-state natural ILC2s and inflammatory ILC2s, which are elicited following helminth infection. However, how tissue-specific cues regulate these two subsets of ILC2s and their effector functions remains elusive. Here, we ... More
Exercise Training Alters Red Blood Cell Fatty Acid Desaturase Indices and Adipose Tissue Fatty Acid Profile in African Women with Obesity.
Allelic variant in the glucagon-like peptide 1 receptor gene associated with greater effect of liraglutide and exenatide on gastric emptying: A pilot pharmacogenetics study.
AuthorsChedid V,Vijayvargiya P,Carlson P,Van Malderen K,Acosta A,Zinsmeister A,Camilleri M
JournalNeurogastroenterology and motility
PubMed ID29488276
BACKGROUND: Weight loss in response to the long-acting GLP-1 receptor (GLP1R) analog, liraglutide, is correlated with delay in gastric emptying (GE). The aim of this pilot study was to assess whether specific genetic variants in GLP1R or TCF7L2 are associated with delayed GE and weight loss in obese patients treated ... More
Development and Validation of a New TaqMan Real-Time PCR for Detection of 'Candidatus Phytoplasma pruni'.
AuthorsKogej Z,Dermastia M,Mehle N
JournalPathogens (Basel, Switzerland)
PubMed ID32784551
Phytoplasmas of the 16SrIII group are wide spread, and have a broad plant host range. Among these, âCandidatus phytoplasma pruniâ (âCa. P. pruniâ; phytoplasmas of 16SrIII subgroup A) can cause serious diseases in Prunus species and âCa. P. pruniâ-related strains can infect other plant species, including grapevines. In this study, ... More
Differential Expression of IGF1, IGFBP5, MSTN, and MYH1 Across Different Age Classes in American Quarter Horses.
AuthorsPeters S,Wilson J,Boyette J
JournalJournal of equine veterinary science
PubMed ID33077104
Age, but Not Sex, Modulates Foxp3 Expression in the Rat Brain across Development.
AuthorsTaylor MR,Roby CR,Elziny S,Duricy E,Taylor TM,Bowers JM
JournalNeuroscience
PubMed ID32599120
Multi-compartment metabolomics and metagenomics reveal major hepatic and intestinal disturbances in cancer cachectic mice.
BACKGROUND: Cancer cachexia is a multifactorial syndrome characterized by multiple metabolic dysfunctions. Besides the muscle, other organs such as the liver and the gut microbiota may also contribute to this syndrome. Indeed, the gut microbiota, an important regulator of the host metabolism, is altered in the C26 preclinical model of ... More
Loss of 15-lipoxygenase disrupts T(reg) differentiation altering their pro-resolving functions.
Regulatory T-cells (T(regs)) are central in the maintenance of homeostasis and resolution of inflammation. However, the mechanisms that govern their differentiation and function are not completely understood. Herein, we demonstrate a central role for the lipid mediator biosynthetic enzyme 15-lipoxygenase (ALOX15) in regulating key aspects of T(reg) biology. Pharmacological inhibition ... More
Transcriptomic Analysis Identifies New Non-Target Site Glyphosate-Resistance Genes in Conyza bonariensis.
Conyza bonariensis (hairy fleabane) is one of the most problematic and widespread glyphosate-resistant weeds in the world. This highly competitive weed species significantly interferes with crop growth and substantially decreases crop yield. Despite its agricultural importance, the molecular mechanisms of glyphosate resistance are still unknown. The present RNA-Seq study was ... More
Interaction of NKX3.1 and SELENOP genotype with prostate cancer recurrence.
AuthorsDonadio JLS,Liu L,Freeman VL,Ekoue DN,Diamond AM,Bermano G
JournalThe Prostate
PubMed ID30582190
Identification of expressed R-genes associated with leaf spot diseases in cultivated peanut.
AuthorsDang PM,Lamb MC,Bowen KL,Chen CY
JournalMolecular biology reports
PubMed ID30498882
A Mouse Model of PPRV Infection for Elucidating Protective and Pathological Roles of Immune Cells.
The study was aimed at developing an accessible laboratory animal model to elucidate protective and pathological roles of immune mediators during Peste des petits ruminants virus (PPRV) infection. It is because of the critical roles of type I IFNs in anti-viral defense, we assessed the susceptibility of IFN receptor knock ... More
DeepCNV: a deep learning approach for authenticating copy number variations.
Copy number variations (CNVs) are an important class of variations contributing to the pathogenesis of many disease phenotypes. Detecting CNVs from genomic data remains difficult, and the most currently applied methods suffer from an unacceptably high false positive rate. A common practice is to have human experts manually review original ... More
Decreased IL-1β-induced CCL20 response in human iPSC-astrocytes in schizophrenia: Potential attenuating effects on recruitment of regulatory T cells.
Genome-Wide Analysis and Function Prediction of Long Noncoding RNAs in Sheep Pituitary Gland Associated with Sexual Maturation.
AuthorsYang H,Ma J,Wang Z,Yao X,Zhao J,Zhao X,Wang F,Zhang Y
JournalGenes
PubMed ID32192168
Long noncoding RNA (lncRNA) plays a crucial role in the hypothalamic-pituitary-testis (HPT) axis associated with sheep reproduction. The pituitary plays a connecting role in the HPT axis. However, little is known of their expression pattern and potential roles in the pituitary gland. To explore the potential lncRNAs that regulate the ... More
Exposure of honey bee larvae to thiamethoxam and its interaction with Nosema ceranae infection in adult honey bees.
AuthorsTesovnik T,Zorc M,Ristanić M,Glavinić U,Stevanović J,Narat M,Stanimirović Z
Inhibition of Chikungunya virus by an adenosine analog targeting the SAM-dependent nsP1 methyltransferase.
AuthorsMudgal R,Mahajan S,Tomar S
JournalFEBS letters
PubMed ID31623018
Alphaviruses, including Chikungunya (CHIKV) and Venezuelan equine encephalitis virus (VEEV), are among the leading causes of recurrent epidemics all over the world. Alphaviral nonstructural protein 1 (nsP1) orchestrates the capping of nascent viral RNA via its Sâadenosyl methionineâdependent Nâ7âmethyltransferase (MTase) and guanylyltransferase activities. Here, we developed and validated a novel ... More
Design of a Gene Panel to Expose the Versatile Role of Hepatic Stellate Cells in Human Liver Fibrosis.
Authorsvan Dijk F,Hazelhoff CM,Post E,Prins GGH,Rombouts K,Poelstra K,Olinga P,Beljaars L
JournalPharmaceutics
PubMed ID32244897
The pivotal cell involved in the pathogenesis of liver fibrosis, i.e., the activated hepatic stellate cell (HSC), has a wide range of activities during the initiation, progression and even regression of the disease. These HSC-related activities encompass cellular activation, matrix synthesis and degradation, proliferation, contraction, chemotaxis and inflammatory signaling. When ... More
Pptc7 is an essential phosphatase for promoting mammalian mitochondrial metabolism and biogenesis.
Mitochondrial proteins are replete with phosphorylation, yet its functional relevance remains largely unclear. The presence of multiple resident mitochondrial phosphatases, however, suggests that protein dephosphorylation may be broadly important for calibrating mitochondrial activities. To explore this, we deleted the poorly characterized matrix phosphatase Pptc7 from mice using CRISPR-Cas9 technology. Strikingly, ... More
Identification of a Retroelement-Containing Human Transcript Induced in the Nucleus by Vaccination.
AuthorsHonda T,Takemoto K,Ueda K
JournalInternational journal of molecular sciences
PubMed ID31200438
Endogenous retroelements constitute almost half of the mammalian genome. Given that more than 60% of human genomic bases are transcribed, transcripts containing these retroelements may impact various biological processes. However, the physiological roles of most retroelement-containing transcripts are yet to be revealed. Here, we profiled the expression of retroelement-containing human ... More
A recurrent COL6A1 pseudoexon insertion causes muscular dystrophy and is effectively targeted by splice-correction therapies.
The clinical application of advanced next-generation sequencing technologies is increasingly uncovering novel classes of mutations that may serve as potential targets for precision medicine therapeutics. Here, we show that a deep intronic splice defect in the COL6A1 gene, originally discovered by applying muscle RNA sequencing in patients with clinical findings ... More
Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection.
AuthorsBak A,Emerson JB
JournalBMC biotechnology
PubMed ID31699075
BACKGROUND: Most genetically modified (GM) plants contain a promoter, P35S, from the plant virus, Cauliflower mosaic virus (CaMV), and many have a terminator, TNOS, derived from the bacterium, Agrobacterium tumefaciens. Assays designed to detect GM plants often target the P35S and/or TNOS DNA sequences. However, because the P35S promoter is ... More
Differential gene expression of tumor-infiltrating CD33(+) myeloid cells in advanced- versus early-stage colorectal cancer.
AuthorsToor SM,Taha RZ,Sasidharan Nair V,Saleh R,Murshed K,Abu Nada M,Elkord E
JournalCancer immunology, immunotherapy : CII
PubMed ID33000418
Colorectal cancer (CRC) has high mortality rates, especially in patients with advanced disease stages, who often do not respond to therapy. The cellular components of the tumor microenvironment are essentially responsible for dictating disease progression and response to therapy. Expansion of different myeloid cell subsets in CRC tumors has been ... More
Expression of the CGRP Family of Neuropeptides and their Receptors in the Trigeminal Ganglion.
AuthorsEdvinsson L,Grell AS,Warfvinge K
JournalJournal of molecular neuroscience : MN
PubMed ID32086679
The calcitonin gene-related peptide (CGRP) family of neuropeptides, consists of CGRP, adrenomedullin, amylin, and calcitonin. The receptors consist of either calcitonin receptor-like receptor (CLR) or calcitonin receptor (CTR) which for function needs an accessory protein, receptor activity-modifying proteins (RAMPs). CGRP has a pivotal role in primary headaches but the role ... More
Long-Term Waterlogging as Factor Contributing to Hypoxia Stress Tolerance Enhancement in Cucumber: Comparative Transcriptome Analysis of Waterlogging Sensitive and Tolerant Accessions.
AuthorsKęska K,Szcześniak MW,Makałowska I,Czernicka M
JournalGenes
PubMed ID33525400
Waterlogging (WL), excess water in the soil, is a phenomenon often occurring during plant cultivation causing low oxygen levels (hypoxia) in the soil. The aim of this study was to identify candidate genes involved in long-term waterlogging tolerance in cucumber using RNA sequencing. Here, we also determined how waterlogging pre-treatment ... More
Combination of quadruplex qPCR and next-generation sequencing for qualitative and quantitative analysis of the HIV-1 latent reservoir.
HIV-1 infection requires lifelong therapy with antiretroviral drugs due to the existence of a latent reservoir of transcriptionally inactive integrated proviruses. The goal of HIV-1 cure research is to eliminate or functionally silence this reservoir. To this end, there are numerous ongoing studies to evaluate immunological approaches, including monoclonal antibody ... More
Antihyperglycemic effect of rice husk derived xylooligosaccharides in high-fat diet and low-dose streptozotocin-induced type 2 diabetic rat model.
AuthorsKhat-Udomkiri N,Toejing P,Sirilun S,Chaiyasut C,Lailerd N
JournalFood science & nutrition
PubMed ID31993169
Rice husk (RH) is an agricultural waste obtained from rice milling process. Our previous study demonstrated the optimized process of extracting xylooligosaccharides (XOS), a prebiotic that can support the growth and activity of beneficial gut microbiota, from RH. Accumulated evidences indicate that the composition of gut microbiota is involved in ... More
Supragingival mycobiome and inter-kingdom interactions in dental caries.
AuthorsBaraniya D,Chen T,Nahar A,Alakwaa F,Hill J,Tellez M,Ismail A,Puri S,Al-Hebshi NN
JournalJournal of oral microbiology
PubMed ID32158514
Background: Recent studies have reveled the presence of a complex fungal community (mycobiome) in the oral cavity. However, the role of oral mycobiome in dental caries and its interaction with caries-associated bacteria is not yet clear. Methods: Whole-mouth supragingival plaque samples from 30 children (6â10Â years old) with no caries, early ... More
miRNA-dependent regulation of STIM1 expression in breast cancer.
Store-operated Ca(2+) entry (SOCE) has been shown to be important for breast cancer metastasis in xenograft mouse models. The ER Ca(2+) sensor STIM1 and Orai plasma membrane Ca(2+) channels molecularly mediate SOCE. Here we investigate the role of the microRNA machinery in regulating STIM1 expression. We show that STIM1 expression ... More
Expression of glucocorticoid and mineralocorticoid receptor genes co-varies with a stress-related colour signal in barn owls.
AuthorsBéziers P,Ducrest AL,San-Jose LM,Simon C,Roulin A
JournalGeneral and comparative endocrinology
PubMed ID31323230
HEATR1, a novel interactor of Pontin/Reptin, stabilizes Pontin/Reptin and promotes cell proliferation of oral squamous cell carcinoma.
Comprehensive development is critical for gut macrophages being essential for the intestinal immune system. However, the underlying mechanisms of macrophage development in the colon remain elusive. To investigate the function of branched-chain amino acids in the development of gut macrophages, an inducible knock-out mouse model for the branched-chain amino acid ... More
Influence of the concentration of dietary digestible calcium on growth performance, bone mineralization, plasma calcium, and abundance of genes involved in intestinal absorption of calcium in pigs from 11 to 22 kg fed diets with different concentrations of digestible phosphorus.
JournalJournal of animal science and biotechnology
PubMed ID31149337
BACKGROUND: A 21-day experiment was conducted to test the hypothesis that Ca requirements to maximize growth performance expressed as the standardized total tract digestible (STTD) Ca to STTD P ratio is less than 1.40:1. The second hypothesis was that increasing dietary Ca increases plasma Ca concentration and downregulates abundance of ... More
Serine Supports IL-1β Production in Macrophages Through mTOR Signaling.
AuthorsChen S,Xia Y,He F,Fu J,Xin Z,Deng B,He L,Zhou X,Ren W
JournalFrontiers in immunology
PubMed ID32973770
Intracellular metabolic programs tightly regulate the functions of macrophages, and previous studies have shown that serine mainly shapes the macrophage function via one-carbon metabolism. However, it is unknown whether serine modulates the macrophage function independent of one-carbon metabolism. Here, we find that serine deprivation lowers interleukin (IL)-1β production and inflammasome ... More
The initial inoculation ratio regulates bacterial coculture interactions and metabolic capacity.
AuthorsGao CH,Cao H,Cai P,Sørensen SJ
JournalThe ISME journal
PubMed ID32887945
Coculture is an important model system in microbial ecology studies. As a key experimental parameter, the initial inoculation ratio has a crucial impact on the results of the coculture system. However, such an effect has never been investigated under multiple niche conditions. In this study, we established a simple coculture ... More
Adaptation of Mitochondrial Substrate Flux in a Mouse Model of Nonalcoholic Fatty Liver Disease.
AuthorsStaňková P,Kučera O,Peterová E,Lotková H,Maseko TE,Nožičková K,Červinková Z
JournalInternational journal of molecular sciences
PubMed ID32046101
Maladaptation of mitochondrial oxidative flux seems to be a considerable feature of nonalcoholic fatty liver disease (NAFLD). The aim of this work was to induce NAFLD in mice fed a Western-style diet (WD) and to evaluate liver mitochondrial functions. Experiments were performed on male C57BL/6J mice fed with a control ... More
Epithelial-to-Mesenchymal Transition Enhances Cancer Cell Sensitivity to Cytotoxic Effects of Cold Atmospheric Plasmas in Breast and Bladder Cancer Systems.
SIMPLE SUMMARY: Cold atmospheric plasma (CAP) and plasma-activated medium (PAM) are known to selectively kill cancer cells, however the efficacy of CAP in cancer cells following epithelial-mesenchymal transition (EMT), a process which endows cancer cells with increased stemness, metastatic potential, and resistance to conventional therapies, has not been previously examined. ... More
Midbrain organoids with an SNCA gene triplication model key features of synucleinopathy.
SNCA, the first gene associated with Parkinsonâs disease, encodes the α-synuclein protein, the predominant component within pathological inclusions termed Lewy bodies. The presence of Lewy bodies is one of the classical hallmarks found in the brain of patients with Parkinsonâs disease, and Lewy bodies have also been observed in patients ... More
Regulatory Small RNA Qrr2 Is Expressed Independently of Sigma Factor-54 and Can Function as the Sole Qrr Small RNA To Control Quorum Sensing in Vibrio parahaemolyticus.
AuthorsTague JG,Hong J,Kalburge SS,Boyd EF
JournalJournal of bacteriology
PubMed ID34633869
Bacterial cells alter gene expression in response to changes in population density in a process called quorum sensing (QS). In Vibrio harveyi, LuxO, a low-cell-density activator of sigma factor-54 (RpoN), is required for transcription of five noncoding regulatory small RNAs (sRNAs), Qrr1 to Qrr5, which each repress translation of the ... More
Identification, Characterization, and Expression Profile Analysis of the mTERF Gene Family and Its Role in the Response to Abiotic Stress in Barley (Hordeum vulgare L.).
AuthorsLi T,Pan W,Yuan Y,Liu Y,Li Y,Wu X,Wang F,Cui L
JournalFrontiers in plant science
PubMed ID34335653
Plant mitochondrial transcription termination factor (mTERF) family regulates organellar gene expression (OGE) and is functionally characterized in diverse species. However, limited data are available about its functions in the agriculturally important cereal barley (Hordeum vulgare L.). In this study, we identified 60 mTERFs in the barley genome (HvmTERFs) through a ... More