Oncomine™ TCR Beta-LR Assay GX - FAQs

查看更多产品信息 Oncomine™ TCR Beta-LR Assay GX - FAQs (A46297)

5 个常见问题解答

What is included in Oncomine TCR Beta-LR Assay GX?

Oncomine TCR Beta-LR Assay GX includes the following:
- TCRB‑LR RNA Pool 1 panel (8 x 2 tubes, 70 ul/tube), for 32 sequencing reactions (4 reactions/tube pair)
- Genexus Strip 1 (8 strips), for 32 sequencing reactions (4 reactions/strip)
- Genexus Strip 2-AS (8 strips), for 32 sequencing reactions (4 reactions/strip)

Note:
- The TCRB-LR RNA Pool 1 is provided in 8 pairs of tubes, where each tube pair contains one tube with the panel in position 1 and one empty uncapped tube in position 2.
- Genexus Library Strips 1 and 2-AS combo kit (Cat. No. A40252) can be purchased separately.

How many sequencing reactions can I perform with Oncomine TCR Beta-LR Assay GX?

The assay kit provides the targeted single pool multiplex panel and library reagents sufficient to perform up to 32 sequencing reactions.

How much of each sample do I need for Oncomine TCR Beta-LR Assay GX (Cat. No. A46297)?

Flexible RNA input amount of 10 ng (minimum) and up to 1 µg supports the identification of rare clones as well as abundant clones.

Which sample types are compatible with Oncomine TCR Beta-LR Assay GX (Cat. No. A46297)?

Oncomine TCR Beta-LR Assay GX is compatible with fresh-frozen tissue, whole blood, and sorted cells. The assay is optimized for long-read sequencing on the Genexus Integrated Sequencer, using the Genexus GX5 Chip.

What is the goal of Oncomine TCR Beta-LR Assay GX (Cat. No. A46297) ?

Oncomine TCR Beta-LR Assay GX is designed to measure T cell diversity and clonal expansion by sequencing T cell receptor beta chain rearrangements. It is designed to capture all three complementarity-determining regions of the TCR beta chain (CDR1, CDR2, and CDR3) with high accuracy. The assay is optimized for long-read sequencing on the Genexus Integrated Sequencer, using the Genexus GX5 Chip.