KingFisher™ PlasmidPro Maxi Processor Endotoxin-Free Cartridge - FAQs

Can I resuspend a frozen pellet for use with the KingFisher PlasmidPro Maxi Processor?

When using frozen pellets as a starting point, please consider the following guidelines:

• The pellet should represent 150 mL of the specified culture.
• Fully resuspend the pellet in 150 mL of 1X PBS or the parent media.
• The resuspension can be achieved by either pipetting up and down or vigorous vortexing.

What are the storage conditions for the KingFisher PlasmidPro Maxi Processor Cartridge? Is refrigeration required?

We recommend storing the KingFisher PlasmidPro Maxi Processor Cartridge at room temperature. Refrigeration is not needed.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Can I reuse the KingFisher PlasmidPro Maxi Processor Cartridge?

The KingFisher PlasmidPro Maxi Processor Cartridge is single-use and should be disposed of after use, minimizing clean-up and risk of cross-contamination. Re-use of the cartridge will contaminate and potentially harm the instrument.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Is the KingFisher PlasmidPro Maxi Processor Cartridge animal origin-free?

No. The RNaseA used in the KingFisher PlasmidPro Maxi Processor Cartridge is not animal origin-free. Review the Certificate of Analysis (COA) for more details.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

What solution is the plasmid DNA sample resuspended in at the end of the purification using the KingFisher PlasmidPro Maxi Processor?

At the end of the purification using the KingFisher PlasmidPro Maxi Processor, the plasmid DNA sample is resuspended in TE buffer (10 mM Tris-HCl, pH 8.0, 0.1 mM EDTA).

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

What run data is generated and/or captured by the KingFisher PlasmidPro Maxi Processor?

The KingFisher PlasmidPro Maxi Processor is designed to automatically capture the following information for every run:

• Cartridge Lot Number
• Cartridge Expiration Date
• Sample Name
• Sample Run Start Time
• Sample Run Finish Time
• Run Errors (Type/Details)

By automatically reading the barcodes, the KingFisher PlasmidPro Maxi Processor helps ensure accurate and efficient tracking of important information related to the cartridge, samples, and run details.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Is there any required downtime between runs on the KingFisher PlasmidPro Maxi Processor?

No. The KingFisher PlasmidPro Maxi Processor does not require any downtime between runs. The system does not employ any mechanical, optical, or electrical components that have a “wait” time to reset between purification runs. As soon as the used cartridge is removed from the instrument, a new cartridge can be inserted and run immediately.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

What is the total run-time for the purification process using the KingFisher PlasmidPro Maxi Processor?

The run time for the purification process is 73 min.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

What are the guidelines for growing E. coli for optimal yield and purity results when using the KingFisher PlasmidPro Maxi Processor, e.g., OD range, growth media, plasmid size?

• Use overnight Luria-Bertani medium optimally grown for at least 12 hr but not greater than 16 hr. Grow sufficient culture volume to support 150 mL.
• Use bacterial cultures with a measured cell density of approximately 1.5 - 6.0 absorbance at 600 nm (A600).
• Use a high-copy or low-copy plasmid between 3 and 10 kb cultured in Luria-Bertani medium to obtain maximal yield and purity of plasmid DNA.

Note: Cell density or plasmid kb outside of the recommendation will result in reduced yield and purity.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

How does the KingFisher PlasmidPro Maxi Processor work?

The KingFisher PlasmidPro Maxi Processor is designed for fully automated, maxi-scale plasmid purification from cultures grown in Luria-Bertani medium or Terrific Broth. The instrument utilizes a self-contained cartridge pre-filled with the necessary reagents to process the culture by filtration and alkaline lysis, to minimize handling and preparation time. It uses a system of pumps and valves to move the sample and reagents in the cartridge to perform all the steps of the purification process in under 75 mins and provides endotoxin-free plasmid DNA resuspended in TE buffer, at the end of the procedure.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.