How does the quantification solution provided by the Quantifiler Trio and HP DNA Quantification Kits and HID Real-Time PCR Software easily integrate with robotics for an automated workflow?
Robotic integration is enabled by the automation-friendly bundling of the Quantifiler Trio and HP DNA Quantification kits combined with the HID Real-Time PCR Analysis Software, which has been designed to facilitate automation with commonly used robotic platforms. In addition, our Human Identification Professional Services (HPS) team has extensive expertise in developing customized robotic scripts and conducting on-site verification and validation testing to help you achieve optimal performance with a wide range of platforms. The HPS team has already developed scripts for the Quantifiler Trio and HP DNA Quantification kits on several of the most common robotic platforms, and would be happy to consult with your lab on how to efficiently implement these solutions into your workflow. Please contact your local human identification (HID) account manager to learn more.
Can I use the basic SDS v1.2.3 software to analyze data generated with the Quantifiler Trio and HP DNA Quantification kits?
We do not recommend using the basic SDS v1.2.3 software. The Quantifiler Trio and HP DNA Quantification kits were developed and validated using the HID Real-Time PCR Software. The software ensures the optimal performance of new Quantifiler Trio and HP DNA Quantification kit-specific functionality, such as the automatic calculation of M:F ratios, Degradation Index and streamlined setup for the latest STR analysis kits including the Identifiler Plus, NGM Select and GlobalFiler kits.
Is HID Real-Time PCR Software v1.4 compatible with data files generated by the previous software versions?
Yes, the HID Real-Time PCR Analysis Software v1.4 can read and reanalyze data files generated by the previous versions of HID Real-Time PCR Analysis Software.
How does the HID Real-Time PCR Software v1.4 differ from the previous version, v1.3?
HID Real-Time PCR Software v1.4 includes the same feature set as version 1.3 and runs on Windows 10 as well. The difference is that HID Real-Time PCR software v1.4 is designed to be compatible with the latest QuantStudio 5 firmware version 1.5.1.
How is the HID Real-Time PCR Software different from the basic SDS software?
The HID Real-Time PCR Software has been fully validated for human identification. The key workflow features include configurable Quantifiler templates as well as instrument calibration, data quality assessment, and integrated sample normalization/STR reaction setup tools for simplified, faster sample processing from quantification to STR analysis. It can automatically calculate the degradation index, male:female mixture ratio, and dilution values, and includes a number of customizable data quality flags for rapid data review. The HID Real-Time PCR Software uses different analysis algorithms than the SDS v1.2.3 software. In our internal comparison testing, the HID Real-Time PCR Software generated CT values with statistically significant differences compared to the SDS v1.2.3 software.
