HID Real-Time PCR Analysis Software v1.4 - FAQs

How does the quantification solution provided by the Quantifiler Trio and HP DNA Quantification Kits and HID Real-Time PCR Software easily integrate with robotics for an automated workflow?

Robotic integration is enabled by the automation-friendly bundling of the Quantifiler Trio and HP DNA Quantification kits combined with the HID Real-Time PCR Analysis Software, which has been designed to facilitate automation with commonly used robotic platforms. In addition, our Human Identification Professional Services (HPS) team has extensive expertise in developing customized robotic scripts and conducting on-site verification and validation testing to help you achieve optimal performance with a wide range of platforms. The HPS team has already developed scripts for the Quantifiler Trio and HP DNA Quantification kits on several of the most common robotic platforms, and would be happy to consult with your lab on how to efficiently implement these solutions into your workflow. Please contact your local human identification (HID) account manager to learn more.

Can I use the basic SDS v1.2.3 software to analyze data generated with the Quantifiler Trio and HP DNA Quantification kits?

We do not recommend using the basic SDS v1.2.3 software. The Quantifiler Trio and HP DNA Quantification kits were developed and validated using the HID Real-Time PCR Software. The software ensures the optimal performance of new Quantifiler Trio and HP DNA Quantification kit-specific functionality, such as the automatic calculation of M:F ratios, Degradation Index and streamlined setup for the latest STR analysis kits including the Identifiler Plus, NGM Select and GlobalFiler kits.

Is HID Real-Time PCR Software v1.4 compatible with data files generated by the previous software versions?

Yes, the HID Real-Time PCR Analysis Software v1.4 can read and reanalyze data files generated by the previous versions of HID Real-Time PCR Analysis Software.

How does the HID Real-Time PCR Software v1.4 differ from the previous version, v1.3?

HID Real-Time PCR Software v1.4 includes the same feature set as version 1.3 and runs on Windows 10 as well. The difference is that HID Real-Time PCR software v1.4 is designed to be compatible with the latest QuantStudio 5 firmware version 1.5.1.

How is the HID Real-Time PCR Software different from the basic SDS software?

The HID Real-Time PCR Software has been fully validated for human identification. The key workflow features include configurable Quantifiler templates as well as instrument calibration, data quality assessment, and integrated sample normalization/STR reaction setup tools for simplified, faster sample processing from quantification to STR analysis. It can automatically calculate the degradation index, male:female mixture ratio, and dilution values, and includes a number of customizable data quality flags for rapid data review. The HID Real-Time PCR Software uses different analysis algorithms than the SDS v1.2.3 software. In our internal comparison testing, the HID Real-Time PCR Software generated CT values with statistically significant differences compared to the SDS v1.2.3 software.

What are the system requirements for the HID Real-Time PCR Analysis Software?

The HID Real-Time PCR Software is designed to run on both the instrument computer and stand-alone desktop computers, and they must meet the following system requirements:

Recommended requirements

Pentium 4 or compatible processor, 2.0 GHz (minimum 1.2 GHz)
• 1 GB RAM
• One hard drive with 10 GB available
• 20/48X IDE CD-ROM drive
• USB v2.0 (minimum v1.1)
• Ethernet network interface adapter (10BASE-T)

Compatible operating systems
• Microsoft Windows 10

Firmware
• Gv2.10 for 7500
• HID Real-Time PCR Analysis Software v1.4 requires QuantStudio 5 firmware version 1.5.1

What instrument can I use with the Quantifiler Trio and HP DNA Quantification kits and HID Real-Time PCR Software quantification solution?

The Quantifiler Trio and HP DNA Quantification kits and HID Real-Time PCR Analysis Software v1.3 have been validated to perform on the Applied Biosystems 7500 Real-Time PCR System.*

HID Real-Time PCR Analysis Software v1.4 has been validated to perform on the Applied Biosystems QuantSudio 5 and 7500 Real-Time PCR Systems.

* When used for purposes other than human identification, the instruments cited are for Research Use Only. Not for use in diagnostic procedures.

Is the HID Real-Time PCR Analysis Software compatible with Microsoft Windows 10?

Yes, the HID Real-Time PCR Software versions 1.3 and 1.4 run on both Windows 10.

How do improvements in the Quantifiler Trio DNA Quantification Kit, Quantifiler HP DNA Quantification Kit and HID Real-Time PCR Software correlate with Applied Biosystems STR kits?

The increased robustness (sensitivity, inhibition detection, and M:F ratio) of the Quantifiler HP and Trio chemistries was primarily designed to better correlate with downstream results when used in conjunction with the highly robust Applied Biosystems STR kits. These include Identifiler Plus, NGM SElect, Yfiler Plus, and GlobalFiler kits. The Quantifiler Human, Male, and Duo DNA Quantification Kits correlate more closely with the legacy Identifiler, Profiler, COfiler, SGMPlus, and Yfiler kits in terms of inhibition detection but do not offer any information on DNA degradation. The HID Real-Time PCR Software supports the setup and analysis of all currently available Applied Biosystems STR kits and Quantifiler chemistries.

What new features of the Quantifiler Trio and HP Quantification Kits and HID Real-Time PCR Software enable increased workflow efficiency?

Each new Quantifiler System includes the following:

• Updated chemistry
• Updated protocols for flexible sample processing
• A reduction in standard curve points, enabling more samples to be processed per run
• Faster time-to-results with a 33% reduction in cycling times
• Simple and streamlined instrument set up
• Simple and streamlined data analysis
• Sample normalization and STR set-up tables