RNAqueous™ 总 RNA 分离试剂盒
RNAqueous™ 总 RNA 分离试剂盒
Invitrogen™

RNAqueous™ 总 RNA 分离试剂盒

The RNAqueous Total RNA Isolation Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 10^2–10^7 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.
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货号数量产品类型
AM191250 次制备试剂盒
AM10051G50/包Spin Columns
货号 AM1912
价格(CNY)
4,798.00
Each
添加至购物车
数量:
50 次制备
产品类型:
试剂盒
价格(CNY)
4,798.00
Each
添加至购物车
The RNAqueous Total RNA Isolation Kit is for phenol-free total RNA isolation from 1–75 mg of tissue or from 102–107 cells, using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.

The RNAqueous kit provides:

  • Convenient syringe-based filtration
  • Compatibility with vacuum manifolds when processing multiple samples

How it works

The RNAqueous kit uses a powerful chaotropic solution for cell lysis, followed by RNA binding to glass fiber housed in a luer-lock syringe filter. Washing and elution solutions are simply pushed through the filter using a standard syringe (not included). Vacuum filtration is not required; however, the filter units are compatible with standard vacuum manifolds for processing several samples at once.

仅供科研使用。不可用于诊断程序。
规格
洗脱体积50–120 μL
最终产品类型总 RNA、转录组 RNA、micro RNA
适用于(应用)RT-PCR、qPCR、cDNA 文库构建、NGS、微阵列分析、印迹杂交、Northern 印迹、体外翻译、核酸酶保护测定、核酸标记
高通量能力不兼容高通量应用(手动)
产品类型试剂盒
数量50 次制备
运输条件室温
原始材料量细菌:≤109 个细胞
细胞:≤107
植物:≤75 mg
酵母菌:≤108 个细胞
病毒:各种
酶促反应:各种
分离技术离心柱(玻璃纤维滤器)
样品类型细菌、细胞、植物、组织、酵母菌、病毒、酶促反应, 细胞, 植物样品, 组织, 病毒样品, 酵母菌, Enzymatic Reactions
Unit SizeEach
内容与储存
• 6 mL 甲醛上样染料;在 -20°C 下储存
• 60 mL 裂解/结合溶液;在 4°C 下储存
• 60 mL 水;在 4°C 下储存
• 4 mL 氯化锂沉淀溶液;在 4°C 下储存
• 40 mL 洗涤溶液 #1;在 4°C 下储存
• 80 mL 洗涤液 #2/3(浓缩液);在 4°C 下储存
• 50个滤芯;在室温下储存
• 100个收集管;在室温下储存
• 10 mL 洗脱溶液;在室温下储存

常见问题解答 (FAQ)

使用RNaqueous试剂盒提取的RNA分子最短是多少?

使用RNAqeous家族的试剂盒提取的为200 nt以上的RNA,所以这些试剂盒不适合提取5S rRNA 或tRNA。但是,RNAqueous-Micro Total RNA Isolation试剂盒提供一个改进的实验方案,可以从样本分离miRNA。

我在使用你们的RNAqueous总RNA分离试剂盒时,滤膜发生了堵塞,对此你们有什么建议吗?

为防止滤膜被粘稠样品或含大量组织的样品堵塞,应先以10,000–15,000 x g的转速离心净化,并将上清液转移到新管子中,再向裂解/结合液中加入乙醇。

不同的RNAqueous试剂盒有何区别?

RNaqueous试剂盒是基于快速的过滤法RNA分离系统,不需要苯酚、氯仿或其他有毒有机试剂。RNAqueous总RNA分离试剂盒专用于10–75 mg的组织或10^6-10^7的细胞样品,而经过优化的RNAqueous-Midi总RNA分离试剂盒是用来从多至0.5g组织或10^9个细胞样品中提取总RNA的试剂盒。RNAqueous-Micro微量总RNA提取试剂盒可用于从微量样本中纯化总RNA,如10–500,000个培养细胞、少至10个显微切割细胞或最多10 mg的组织。RNAqueous-96总RNA分离试剂盒利用96孔板格式,对100至2 x 10^6个细胞或0.1至1.5 mg组织进行高通量RNA分离。RNAqueous-4PCR总RNA分离试剂盒专用于从少量组织样品(0.5–75 mg)以及少量至中量培养细胞样品(约100–10^7个细胞)中分离RNA。

What is the minimum RNA size that is isolated using RNaqueous kits?

RNAs less than 200 nt are lost using the columns in the RNAqeous family of kits. These kits are not suitable for isolation of 5S rRNA or tRNA. The RNAqueous-Micro Total RNA Isolation Kit does, however, offer a modified protocol to allow isolation of miRNA from the sample.

I'm using your RNAqueous Total RNA Isolation Kit , and the filter is clogging. Do you have any suggestions?

To prevent filter clogging with viscous samples or samples containing a large amount of tissue, perform a clarifying centrifugatoin at 10,000-15,000 x g and remove to a fresh tube prior to adding ethanol to the lysis/binding solution.