RNAqueous™ Total RNA Isolation Kit - FAQs

View additional product information for RNAqueous™ Total RNA Isolation Kit - FAQs (AM10051G, AM1912)

6 product FAQs found

使用RNaqueous试剂盒提取的RNA分子最短是多少？

使用RNAqeous家族的试剂盒提取的为200 nt以上的RNA，所以这些试剂盒不适合提取5S rRNA 或tRNA。但是，RNAqueous-Micro Total RNA Isolation试剂盒提供一个改进的实验方案，可以从样本分离miRNA。

我在使用你们的RNAqueous总RNA分离试剂盒时，滤膜发生了堵塞，对此你们有什么建议吗？

为防止滤膜被粘稠样品或含大量组织的样品堵塞，应先以10,000–15,000 x g的转速离心净化，并将上清液转移到新管子中，再向裂解/结合液中加入乙醇。

不同的RNAqueous试剂盒有何区别？

RNaqueous试剂盒是基于快速的过滤法RNA分离系统，不需要苯酚、氯仿或其他有毒有机试剂。RNAqueous总RNA分离试剂盒专用于10–75 mg的组织或10^6-10^7的细胞样品，而经过优化的RNAqueous-Midi总RNA分离试剂盒是用来从多至0.5g组织或10^9个细胞样品中提取总RNA的试剂盒。RNAqueous-Micro微量总RNA提取试剂盒可用于从微量样本中纯化总RNA，如10–500,000个培养细胞、少至10个显微切割细胞或最多10 mg的组织。RNAqueous-96总RNA分离试剂盒利用96孔板格式，对100至2 x 10^6个细胞或0.1至1.5 mg组织进行高通量RNA分离。RNAqueous-4PCR总RNA分离试剂盒专用于从少量组织样品（0.5–75 mg）以及少量至中量培养细胞样品（约100–10^7个细胞）中分离RNA。

What is the minimum RNA size that is isolated using RNaqueous kits?

RNAs less than 200 nt are lost using the columns in the RNAqeous family of kits. These kits are not suitable for isolation of 5S rRNA or tRNA. The RNAqueous-Micro Total RNA Isolation Kit does, however, offer a modified protocol to allow isolation of miRNA from the sample.

I'm using your RNAqueous Total RNA Isolation Kit , and the filter is clogging. Do you have any suggestions?

To prevent filter clogging with viscous samples or samples containing a large amount of tissue, perform a clarifying centrifugatoin at 10,000-15,000 x g and remove to a fresh tube prior to adding ethanol to the lysis/binding solution.

What are the differences in the RNAqueous kits you offer?

RNaqueous kits are based on a rapid, filter-based RNA isolation system that does not require phenol, chloroform, or other toxic organic chemicals. The RNAqueous Total RNA Isolation Kit is designed for sample sizes of 10-75 mg of tissue or 10^6 to 10^7 cells, whereas the RNAqueous-Midi Total RNA Isolation Kit is designed for tissue samples of up to 0.5 g or 10^9 cells. The RNAqueous-Micro Total RNA Isolation Kit is optimized for the purification of total RNA from micro-sized samples such as 10-500,000 cultured cells, as few as 10 microdissected cells, or up to 10 mg tissue. The RNAqueous-96 Total RNA Isolation Kit utilizes a 96-well plate format for high-throughput RNA isolation from 100 to 2 x 10^6 cells or 0.1 to 1.5 mg of tissue. The RNAqueous-4PCR Total RNA Isolation Kit is designed for RNA isolation from small tissue samples (0.5-75 mg) and small- to medium-sized cultured cell samples (approximately 100-10^7 cells).