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View additional product information for LeukoLOCK™ Total RNA Isolation System - FAQs (AM1923)
14 product FAQs found
是的。您将需要按照另一个实验方案操作，点击此链接（https://www.thermofisher.com/cn/zh/home/references/protocols/nucleic-acid-purification-and-analysis/rna-protocol/alternative-protocol-extraction-of-rna-from-cells-captured-on-leukolock-filters-using-tri-reagent.html）获取。
我们使用LeukoLOCK总RNA分离系统处理过用肝素保存的血液。RNA看起来没有问题。但是，我们尚未测试将其用于任何下游应用。众所周知，肝素可以抑制RT-PCR等下游应用，所以我们不建议您使用肝素。
不兼容，它们是基于不同的化学反应。
LeukoLOCK总RNA分离系统仅经过人类血液的测试。
不能。尚未验证将该试剂盒用于少于3 mL的人类血液。最好使用9–10 mL血液。
10 mL血液的RNA产量通常为10–20 µg。RNA质量取决于血液质量。一般来说，RNA完整指数（RIN）约为9，28S/18S比值约为1.5。
从未预先分离的全血中提取的mRNA样品通常含有网织红细胞来源的的α和β-球蛋白mRNA，使用LeukoLOCK总RNA分离系统提取的RNA中通常含有少于10%的此类mRNA。分离的RNA特别适用于基因表达谱分析，因为它含有更低比例的球蛋白mRNA。我们的LeukoLOCK组分分离和稳定试剂盒（货号AM1933），可用于血液收集和稳定。之后客户可以使用LeukoLOCK总RNA分离试剂盒（货号AM1934）可分离在LeukoLOCK过滤器上稳定的RNA。在LeukoLOCK总RNA分离系统（货号AM1923）试剂盒中同时包含AM1934和AM1933两个产品，可一起使用。
Yes. You will need to follow the alternate protocol, available in the following link.
We have processed blood stored with heparin with the LeukoLOCK Total RNA Isolation System. The RNA looks fine. But we have not tested it for any downstream applications. We discourage your use of heparin, as heparin notoriously inhibits downstream applications such as RT-PCR.
No, they are based on different chemistries.
The LeukoLOCK Total RNA Isolation System has been tested only for human blood.
No. The kit has not been validated for less than 3 mL of human blood. It is best to use 9-10 mL blood.
RNA yield is typically 10-20 µg from 10 mL of blood. RNA quality depends on the quality of the blood. Typically, the RNA integrity number (RIN) value is around 9, and the 28S/18S ratio is around 1.5.
RNA recovered using the LeukoLOCK Total RNA Isolation System typically contains less than 10% of the amount of reticulocyte-derived alpha- and beta-globin mRNAs present in typical mRNA samples from unfractionated whole blood. The isolated RNA is specially suited for gene expression profiling, as it contains a lower proportion of globin mRNA. We sell LeukoLOCK Fractionation and Stabilization Kit (Cat. No. AM1933) for blood collection and stabilization. You can then use LeukoLOCK Total RNA Isolation Kit (Cat. No. AM1934) to isolate RNA stabilized on the LeukoLOCK filters. Cat. Nos. AM1934 and AM1933 are available together, packaged in a single kit as the LeukoLOCK Total RNA Isolation System (Cat. No. AM1923).