The panniculus carnosus muscle: A novel model of striated muscle regeneration that exhibits sex differences in the mdx mouse.
AuthorsBahri OA, Naldaiz-Gastesi N, Kennedy DC, Wheatley AM, Izeta A, McCullagh KJA,
JournalSci Rep
PubMed ID31685850
'The dermal striated muscle panniculus carnosus (PC), prevalent in lower mammals with remnants in humans, is highly regenerative, and whose function is purported to be linked to defence and shivering thermogenesis. Given the heterogeneity of responses of different muscles to disease, we set out to characterize the PC in wild-type ... More
Negative Regulation of TLR Signaling by BCAP Requires Dimerization of Its DBB Domain.
'The B cell adaptor protein (BCAP) is a multimodular regulator of inflammatory signaling in diverse immune system cells. BCAP couples TLR signaling to phosphoinositide metabolism and inhibits MyD88-directed signal transduction. BCAP is recruited to the TLR signalosome forming multitypic interactions with the MAL and MyD88 signaling adaptors. In this study, ... More
Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19.
AuthorsBlanco-Melo D, Nilsson-Payant BE, Liu WC, Uhl S, Hoagland D, Møller R, Jordan TX, Oishi K, Panis M, Sachs D, Wang TT, Schwartz RE, Lim JK, Albrecht RA, tenOever BR,
JournalCell
PubMed ID32416070
'Viral pandemics, such as the one caused by SARS-CoV-2, pose an imminent threat to humanity. Because of its recent emergence, there is a paucity of information regarding viral behavior and host response following SARS-CoV-2 infection. Here we offer an in-depth analysis of the transcriptional response to SARS-CoV-2 compared with other ... More
Pathogenesis of SARS-CoV-2 in Transgenic Mice Expressing Human Angiotensin-Converting Enzyme 2.
AuthorsJiang RD, Liu MQ, Chen Y, Shan C, Zhou YW, Shen XR, Li Q, Zhang L, Zhu Y, Si HR, Wang Q, Min J, Wang X, Zhang W, Li B, Zhang HJ, Baric RS, Zhou P, Yang XL, Shi ZL,
JournalCell
PubMed ID32516571
'COVID-19 has spread worldwide since 2019 and is now a severe threat to public health. We previously identified the causative agent as a novel SARS-related coronavirus (SARS-CoV-2) that uses human angiotensin-converting enzyme 2 (hACE2) as the entry receptor. Here, we successfully developed a SARS-CoV-2 hACE2 transgenic mouse (HFH4-hACE2 in C3B6 ... More
Sirtuin 6 deficiency induces endothelial cell senescence via downregulation of forkhead box M1 expression.
AuthorsLee OH, Woo YM, Moon S, Lee J, Park H, Jang H, Park YY, Bae SK, Park KH, Heo JH, Choi Y,
JournalAging (Albany NY)
PubMed ID33171439
'Cellular senescence of endothelial cells causes vascular dysfunction, promotes atherosclerosis, and contributes to the development of age-related vascular diseases. Sirtuin 6 (SIRT6), a conserved NAD+-dependent protein deacetylase, has beneficial effects against aging, despite the fact that its functional mechanisms are largely uncharacterized. Here, we show that SIRT6 protects endothelial cells ... More
Engineering Intracellular Protein Sensors in Mammalian Cells.
AuthorsDuhkinova M, Crina C, Weiss R, Siciliano V,
JournalJ Vis Exp
PubMed ID32420982
'Proteins can function as biomarkers of pathological conditions, such as neurodegenerative diseases, infections or metabolic syndromes. Engineering cells to sense and respond to these biomarkers may help the understanding of molecular mechanisms underlying pathologies, as well as to develop new cell-based therapies. While several systems that detect extracellular proteins have ... More
R-spondins engage heparan sulfate proteoglycans to potentiate WNT signaling.
AuthorsDubey R, van Kerkhof P, Jordens I, Malinauskas T, Pusapati GV, McKenna JK, Li D, Carette JE, Ho M, Siebold C, Maurice M, Lebensohn AM, Rohatgi R,
JournalElife
PubMed ID32432544
'R-spondins (RSPOs) amplify WNT signaling during development and regenerative responses. We previously demonstrated that RSPOs 2 and 3 potentiate WNT/ß-catenin signaling in cells lacking leucine-rich repeat-containing G-protein coupled receptors (LGRs) 4, 5 and 6 (Lebensohn and Rohatgi, 2018). We now show that heparan sulfate proteoglycans (HSPGs) act as alternative co-receptors ... More
Long Non-Coding RNA Nuclear-Enriched Abundant Transcript 1 (NEAT1) Represses Proliferation of Trophoblast Cells in Rats with Preeclampsia via the MicroRNA-373/FLT1 Axis.
AuthorsTeng L, Liu P, Song X, Wang H, Sun J, Yin Z,
JournalMed Sci Monit
PubMed ID33093438
'BACKGROUND Preeclampsia (PE) remains one of the primary causes of maternal morbidity and mortality worldwide. This study was designed to investigate the relevance of long non-coding RNA (lncRNA) nuclear-enriched abundant transcript 1 (NEAT1) and downstream molecules in trophoblast cell proliferation and apoptosis. MATERIAL AND METHODS NEAT1 expression in the placental ... More
TOPORS, a tumor suppressor protein, contributes to the maintenance of higher-order chromatin architecture.
AuthorsJi L, Huo X, Zhang Y, Yan Z, Wang Q, Wen B,
JournalBiochim Biophys Acta Gene Regul Mech
PubMed ID32113985
'In the nucleus, chromosomes are hierarchically folded into active (A) and inactive (B) compartments composed of topologically associating domains (TADs). Genomic regions interact with nuclear lamina, termed lamina-associated domains (LADs). However, the molecular mechanisms underlying these 3D chromatin architectures remain incompletely understood. Here, we investigated the role of a potential ... More
The Killing of Human Neuroblastoma Cells by the Small Molecule JQ1 Occurs in a p53-Dependent Manner.
AuthorsMazar J, Gordon C, Naga V, Westmoreland TJ,
JournalAnticancer Agents Med Chem
PubMed ID32329693
'MYCN amplification is a prognostic biomarker associated with poor prognosis of neuroblastoma in children. The overall survival of children with MYCN-amplified neuroblastoma has only marginally improved within the last 20 years. The Bromodomain and Extra-Terminal motif (BET) inhibitor, JQ1, has been shown to downregulate MYCN in neuroblastoma cells. To determine ... More
SON and SRRM2 are essential for nuclear speckle formation.
'Nuclear speckles (NS) are among the most prominent biomolecular condensates. Despite their prevalence, research on the function of NS is virtually restricted to colocalization analyses, since an organizing core, without which NS cannot form, remains unidentified. The monoclonal antibody SC35, raised against a spliceosomal extract, is frequently used to mark ... More
Cephalopod-inspired optical engineering of human cells.
'Although many animals have evolved intrinsic transparency for the purpose of concealment, the development of dynamic, that is, controllable and reversible, transparency for living human cells and tissues has remained elusive to date. Here, by drawing inspiration from the structures and functionalities of adaptive cephalopod skin cells, we design and ... More
3D Printed Polyurethane Scaffolds for the Repair of Bone Defects.
AuthorsCooke ME, Ramirez-GarciaLuna JL, Rangel-Berridi K, Park H, Nazhat SN, Weber MH, Henderson JE, Rosenzweig DH,
JournalFront Bioeng Biotechnol
PubMed ID33195122
'Critical-size bone defects are those that will not heal without intervention and can arise secondary to trauma, infection, and surgical resection of tumors. Treatment options are currently limited to filling the defect with autologous bone, of which there is not always an abundant supply, or ceramic pastes that only allow ... More
Photothermal-Induced Antibacterial Activity of Gold Nanorods Loaded into Polymeric Hydrogel against Pseudomonas aeruginosa Biofilm.
AuthorsAl-Bakri AG, Mahmoud NN,
JournalMolecules
PubMed ID31340472
'In this study, the photothermal-induced bactericidal activity of phospholipid-decorated gold nanorods (DSPE-AuNR) suspension against Pseudomonas aeruginosa planktonic and biofilm cultures was investigated. We found that the treatment of planktonic culture of Pseudomonas aeruginosa with DSPE-AuNR suspension (0.25-0.03 nM) followed by a continuous laser beam exposure resulted in ~6 log cycle ... More
ZNF521 Represses Osteoblastic Differentiation in Human Adipose-Derived Stem Cells.
AuthorsChiarella E, Aloisio A, Scicchitano S, Lucchino V, Montalcini Y, Galasso O, Greco M, Gasparini G, Mesuraca M, Bond HM, Morrone G,
JournalInt J Mol Sci
PubMed ID30567301
Human adipose-derived stem cells (hADSCs) are multipotent mesenchymal cells that can differentiate into adipocytes, chondrocytes, and osteocytes. During osteoblastogenesis, the osteoprogenitor cells differentiate into mature osteoblasts and synthesize bone matrix components. Zinc finger protein 521 (ZNF521/Zfp521) is a transcription co-factor implicated in the regulation of hematopoietic, neural, and mesenchymal stem ... More
Polyvinylidene fluoride-Hyaluronic acid wound dressing comprised of ionic liquids for controlled drug delivery and dual therapeutic behavior.
AuthorsAbednejad A, Ghaee A, Morais ES, Sharma M, Neves BM, Freire MG, Nourmohammadi J, Mehrizi AA,
JournalActa Biomater
PubMed ID31586728
To improve the efficacy of transdermal drug delivery systems, the physical and chemical properties of drugs need to be optimized to better penetrate into the stratum corneum and to better diffuse into the epidermis and dermis layers. Accordingly, dual-biological function ionic liquids composed of active pharmaceutical ingredients were synthesized, comprising ... More
Decreased expression of miR-551b predicts poor prognosis and promotes tumorigenesis by targeting PTP4A3 in human colorectal cancer.
AuthorsSun HY, Qu ZC, Liu DM, Zhang W, Liu G, Zhu L,
JournalEur Rev Med Pharmacol Sci
PubMed ID31298325
MiR-551b has been reported to display tumor-suppressive and oncogenic potential in several cancers, but there has been no study on the roles of miR-551b in colorectal cancer (CRC). In this work, we aimed to explore the potential functions and mechanisms of miR-551b in the modulation of the CRC progression. The ... More
Three-dimensional encapsulation of adult mouse cardiomyocytes in hydrogels with tunable stiffness.
Numerous diseases, including those of the heart, are characterized by increased stiffness due to excessive deposition of extracellular matrix proteins. Cardiomyocytes continuously adapt their morphology and function to the mechanical changes of their microenvironment. Because traditional cell culture is conducted on substrates that are many orders of magnitude stiffer than ... More
STAT3-induced long noncoding RNA LINC00668 promotes migration and invasion of non-small cell lung cancer via the miR-193a/KLF7 axis.
AuthorsAn YX, Shang YJ, Xu ZW, Zhang QC, Wang Z, Xuan WX, Zhang XJ,
JournalBiomed Pharmacother
PubMed ID31150989
Long noncoding RNAs (lncRNAs) have been demonstrated to play significant roles in non-small cell lung cancer (NSCLC) progression. Recently, a newly identified lncRNA, LncRNA LINC00668 (LINC00668), was reported to be involved in the regulation of progression of several tumors. However, the expression pattern and biological function of LINC00668 in NSCLC ... More
Dysfunction of iPSC-derived endothelial cells in human Hutchinson-Gilford progeria syndrome.
Children with Hutchinson-Gilford progeria syndrome (HGPS) succumb to myocardial infarction and stroke in their teen years. Endothelial dysfunction is an early event in more common forms of atherosclerosis. Endothelial pathobiology may contribute to HGPS, but a comprehensive characterization of endothelial function in HGPS has not been performed. iPSCs derived from ... More
Porcine circovirus type 2 promotes Actinobacillus pleuropneumoniae survival during coinfection of porcine alveolar macrophages by inhibiting ROS production.
AuthorsQi W, Zhu R, Bao C, Xiao J, Liu B, Sun M, Feng X, Gu J, Li Y, Lei L,
JournalVet Microbiol
PubMed ID31176418
Actinobacillus pleuropneumoniae (APP) and porcine circovirus type 2 (PCV2) are both important pathogens of the porcine respiratory disease complex (PRDC), which results in significant worldwide economic losses. Recently, PCV2 and APP coinfection has been described in the worldwide pork industry, and represents an extremely complex situation in veterinary medicine. However, ... More
Notoginseng Leaf Triterpenes Ameliorates OGD/R-Induced Neuronal Injury via SIRT1/2/3-Foxo3a-MnSOD/PGC-1a Signaling Pathways Mediated by the NAMPT-NAD Pathway.
AuthorsXie W, Zhu T, Zhou P, Xu H, Meng X, Ding T, Nan F, Sun G, Sun X,
JournalOxid Med Cell Longev
PubMed ID33149812
Cerebral ischemic stroke (CIS) is a common cerebrovascular disease whose main risks include necrosis, apoptosis, and cerebral infarction. But few therapeutic advances and prominent drugs seem to be of value for ischemic stroke in the clinic yet. In the previous study, notoginseng leaf triterpenes (PNGL) from Panax notoginseng stem and ... More
Characterization of Uveal Melanoma Cell Lines and Primary Tumor Samples in 3D Culture.
AuthorsAughton K, Shahidipour H, Djirackor L, Coupland SE, Kalirai H,
JournalTransl Vis Sci Technol
PubMed ID32832244
Uveal melanoma (UM) typically spreads to the liver, where it is incurable, as there are limited therapeutic interventions available. This study aimed to standardize laboratory methods for generating three-dimensional (3D) spheroids using UM cell lines and primary UM (PUM) samples for use in drug screening. Six UM cell lines and ... More
Doxycycline has distinct apicoplast-specific mechanisms of antimalarial activity.
AuthorsOkada M, Guo P, Nalder SA, Sigala PA,
JournalElife
PubMed ID33135634
Doxycycline (DOX) is a key antimalarial drug thought to kill Plasmodium parasites by blocking protein translation in the essential apicoplast organelle. Clinical use is primarily limited to prophylaxis due to delayed second-cycle parasite death at 1-3 µM serum concentrations. DOX concentrations > 5 µM kill parasites with first-cycle activity but ... More
Transcriptional repression of SIRT3 potentiates mitochondrial aconitase activation to drive aggressive prostate cancer to the bone.
AuthorsSawant Dessai A, Palestino Dominguez M, Chen UI, Hasper J, Prechtl C, Yu C, Katsuta E, Dai T, Zhu B, Jung SY, Putluri N, Takabe K, Zhang XH, O'Malley BW, Dasgupta S,
JournalCancer Res
PubMed ID33115805
Metabolic dysregulation is a known hallmark of cancer progression, yet the oncogenic signals that promote metabolic adaptations to drive metastatic cancer remain unclear. Here we show that transcriptional repression of mitochondrial deacetylase sirtuin 3 (SIRT3) by androgen receptor (AR) and its coregulator steroid receptor coactivator (SRC-2) enhances mitochondrial aconitase (ACO2) ... More
Alzheimer's amyloid ß heterogeneous species differentially affect brain endothelial cell viability, blood-brain barrier integrity, and angiogenesis.
AuthorsParodi-Rullán R, Ghiso J, Cabrera E, Rostagno A, Fossati S,
JournalAging Cell
PubMed ID33155752
Impaired clearance in the Alzheimer's Disease (AD) brain is key in the formation of Aß parenchymal plaques and cerebrovascular deposits known as cerebral amyloid angiopathy (CAA), present in >80% of AD patients and ~50% of non-AD elderly subjects. Aß deposits are highly heterogeneous, containing multiple fragments mostly derived from catabolism ... More
Real-time tracking of stem cell viability, proliferation, and differentiation with autonomous bioluminescence imaging.
AuthorsConway M, Xu T, Kirkpatrick A, Ripp S, Sayler G, Close D,
JournalBMC Biol
PubMed ID32620121
Luminescent reporter proteins are vital tools for visualizing cells and cellular activity. Among the current toolbox of bioluminescent systems, only bacterial luciferase has genetically defined luciferase and luciferin synthesis pathways that are functional at the mammalian cell temperature optimum of 37?°C and have the potential for in vivo applications. However, ... More
Screening method to identify hydrogel formulations that facilitate myotube formation from encapsulated primary myoblasts.
AuthorsDeshmukh DV, Pasquero N, Rathore G, Zvick J, Bar-Nur O, Dual J, Tibbitt MW,
JournalBioeng Transl Med
PubMed ID33005743
Hydrogel-based three-dimensional (3D) cellular models are attractive for bioengineering and pharmaceutical development as they can more closely resemble the cellular function of native tissue outside of the body. In general, these models are composed of tissue specific cells embedded within a support material, such as a hydrogel. As hydrogel properties ... More
Endothelial cell-derived extracellular vesicles alter vascular smooth muscle cell phenotype through high-mobility group box proteins.
The vascular endothelium and smooth muscle form adjacent cellular layers that comprise part of the vascular wall. Each cell type can regulate the other's structure and function through a variety of paracrine effectors. Extracellular vesicles (EVs) are released from and transit between cells constituting a novel means of cell-cell communication. ... More
Human skin explant model for the investigation of topical therapeutics.
AuthorsNeil JE, Brown MB, Williams AC,
JournalSci Rep
PubMed ID33273665
The development of in vitro and ex vivo models to mimic human illness is important not only for scientific understanding and investigating therapeutic approaches but also to mitigate animal testing and bridge the inter-species translational gap. While in vitro models can facilitate high-throughput and cost-efficient evaluation of novel therapeutics, more ... More
AuthorsLi W, Yu Y, Li D, Xu N, Fang J, Sun Y, Xu M, Wang X, Han X, Zhang X, Lv C, Han F,
JournalInt Immunopharmacol
PubMed ID32058931
Chronic intermittent hypoxia (CIH) is the main symptom of obstructive sleep apnea syndrome (OSAS) and causes neural damage and cognitive deficits via neuroinflammation. Toll-like receptors (TLRs), especially TLR2, play an important role in neuroinflammation. However, the mechanisms by which TLR2 participates in CIH-induced cognitive deficits remain unclear. In this study, ... More
TRIM11 promotes breast cancer cell proliferation by stabilizing estrogen receptor a.
AuthorsTang J, Luo Y, Tian Z, Liao X, Cui Q, Yang Q, Wu G,
JournalNeoplasia
PubMed ID32599554
Breast cancer is the most commonly diagnosed malignancy in female worldwide, over 70% of which are estrogen receptor a (ERa) positive. ERa has a crucial role in the initiation and progression of breast cancer and is an indicator of endocrine therapy, while endocrine resistance is an urgent problem in ER-positive ... More
High frequency acoustic cell stimulation promotes exosome generation regulated by a calcium-dependent mechanism.
AuthorsAmbattu LA, Ramesan S, Dekiwadia C, Hanssen E, Li H, Yeo LY,
JournalCommun Biol
PubMed ID33020585
Exosomes are promising disease diagnostic markers and drug delivery vehicles, although their use in practice is limited by insufficient homogeneous quantities that can be produced. We reveal that exposing cells to high frequency acoustic irradiation stimulates their generation without detriment to cell viability by exploiting their innate membrane repair mechanism, ... More
Golgi Acidification by NHE7 Regulates Cytosolic pH Homeostasis in Pancreatic Cancer Cells.
AuthorsGalenkamp KMO, Sosicka P, Jung M, Recouvreux MV, Zhang Y, Moldenhauer MR, Brandi G, Freeze HH, Commisso C,
JournalCancer Discov
PubMed ID32200349
Cancer cells reprogram their metabolism to meet elevated energy demands and favor glycolysis for energy production. This boost in glycolytic flux supports proliferation, but also generates acid in the form of hydrogen ions that must be eliminated from the cytoplasm to maintain the alkaline intracellular pH (pHi) associated with transformation. ... More
Effect of microfluidic processing on the viability of boar and bull spermatozoa.
AuthorsHamacher T, Berendsen JTW, Kruit SA, Broekhuijse MLWJ, Segerink LI,
JournalBiomicrofluidics
PubMed ID32774586
The use of microfluidics in artificial reproductive technologies for manipulation or assessment of spermatozoa is unique in the sense that it is not always an end point measurement and the sample may be used afterward. During microfluidic processing, spermatozoa are exposed to shear stress, which may harm viability and functioning ... More
Essential oil impregnated luminescent hydroxyapatite: Antibacterial and cytotoxicity studies.
AuthorsBanerjee S, Bagchi B, Pal K, Bhandary S, Kool A, Hoque NA, Biswas P, Thakur P, Das K, Karmakar P, Das S,
JournalMater Sci Eng C Mater Biol Appl
PubMed ID32806309
In this study, porous fluorescent nanocrystalline erbium doped hydroxyapatite (eHAp) was synthesized via hydrothermal assisted co-precipitation method. Eucalyptus oil (EU), frankincense oil (FO), Tea tree oil (TTO), wintergreen oil (WO) were successfully absorbed into eHAp pellet by vacuum filtration technique using Buckner funnel. Phase crystallization, fluorescence property and microstructure of ... More
STAT3-induced up-regulation of lncRNA NEAT1 as a ceRNA facilitates abdominal aortic aneurysm formation by elevating TULP3.
AuthorsCai B, Yang B, Huang D, Wang D, Tian J, Chen F, Wang X,
JournalBiosci Rep
PubMed ID31868202
Long noncoding RNAs (lncRNAs) were viewed as crucial participants in the pathogenesis of abdominal aortic aneurysm (AAA). LncRNA NEAT1 was recognized as an oncogenic gene in various diseases. However, its function and mechanism in AAA were not precisely documented. Here, we explored the functional role and molecular mechanism of NEAT1 ... More
Hydrazinocurcumin and 5-fluorouracil enhance apoptosis and restrain tumorigenicity of HepG2 cells via disrupting the PTEN-mediated PI3K/Akt signaling pathway.
AuthorsZhou Y, Zhang M, Zhang Z, Jia Y, Zhang C, Peng L,
JournalBiomed Pharmacother
PubMed ID32559627
Hydrazinocurcumin (HZC), a curcumin analogue, serves as a tumor suppressor in breast cancer and lung cancer. In this study, we investigate the role and mechanism of HZC in regulating HepG2 cell apoptosis and tumorigenicity, and its synergistic effects with 5-fluorouracil (5-Fu). HepG2 cells were treated with HZC and/or 5-Fu to ... More
Acute SARS-CoV-2 Infection is Highly Cytopathic, Elicits a Robust Innate Immune Response and is Efficiently Prevented by EIDD-2801.
AuthorsWahl A, Gralinski L, Johnson C, Yao W, Kovarova M, Dinnon K, Liu H, Madden V, Krzystek H, De C, White K, Schäfer A, Zaman T, Leist S, Grant P, Gully K, Askin F, Browne E, Jones C, Pickles R, Baric R, Garcia JV,
JournalRes Sq
PubMed ID32995766
All known recently emerged human coronaviruses likely originated in bats. Here, we used a single experimental platform based on human lung-only mice (LoM) to demonstrate efficient in vivo replication of all recently emerged human coronaviruses (SARS-CoV, MERS-CoV, SARS-CoV-2) and two highly relevant endogenous pre-pandemic SARS-like bat coronaviruses. Virus replication in ... More
The intensification of anticancer activity of LFM-A13 by erythropoietin as a possible option for inhibition of breast cancer.
AuthorsRozkiewicz D, Hermanowicz JM, Tankiewicz-Kwedlo A, Sieklucka B, Pawlak K, Czarnomysy R, Bielawski K, Surazynski A, Kalafut J, Przybyszewska A, Koda M, Jakubowska K, Rivero-Muller A, Pawlak D,
JournalJ Enzyme Inhib Med Chem
PubMed ID32912025
Recombinant human erythropoietin (Epo) is an effective and convenient treatment for cancer-related anaemia. In our study for the first time, we evaluated the effect of simultaneous use of Epo and Bruton's tyrosine kinase (BTK) inhibitor LFM-A13 on the viability and tumour development of breast cancer cells. The results demonstrated that ... More
A portable and high-sensitivity optical sensing system for detecting fluorescently labeled enterohaemorrhagic Escherichia coli Shiga toxin 2B-subunit.
AuthorsKim J, Park JY, Park YJ, Park SY, Lee MS, Koo C,
JournalPLoS One
PubMed ID32673369
We developed a stand-alone, real-time optical detection device capable of reading fluorescence intensities from cell samples with high sensitivity and precision, for use as a portable fluorescent sensor for sensing fluorescently labeled enterohemorrhagic Escherichia coli (EHEC) Shiga toxins (Stxs). In general, the signal intensity from the fluorescently labeled Stxs was ... More
AuthorsPark SJ, Lee J, Seo SE, Kim KH, Park CS, Lee SH, Ban HS, Lee BD, Song HS, Kim J, Lee CS, Bae J, Kwon OS,
JournalSci Rep
PubMed ID32111933
In this study, ultrasensitive and precise detection of a representative brain hormone, dopamine (DA), was demonstrated using functional conducting polymer nanotubes modified with aptamers. A high-performance aptasensor was composed of interdigitated microelectrodes (IMEs), carboxylated polypyrrole nanotubes (CPNTs) and DA-specific aptamers. The biosensors were constructed by sequential conjugation of CPNTs and ... More
Phenotypic and functional characterization of first-trimester human placental macrophages, Hofbauer cells.
AuthorsThomas JR, Appios A, Zhao X, Dutkiewicz R, Donde M, Lee CYC, Naidu P, Lee C, Cerveira J, Liu B, Ginhoux F, Burton G, Hamilton RS, Moffett A, Sharkey A, McGovern N,
JournalJ Exp Med
PubMed ID33075123
Hofbauer cells (HBCs) are a population of macrophages found in high abundance within the stroma of the first-trimester human placenta. HBCs are the only fetal immune cell population within the stroma of healthy placenta. However, the functional properties of these cells are poorly described. Aligning with their predicted origin via ... More