Can I visualize Alexa Fluor 488 dye using the EVOS M5000 Imaging System?
Yes, you can visualize Alexa Fluor 488 dye using the EVOS M5000 Imaging System. The system supports the GFP light cube, which is compatible with Alexa Fluor 488 dye. This allows for effective imaging of samples stained with Alexa Fluor 488 dye.
For more details on compatible dyes and light cubes, please refer to page 136 of the EVOS M5000 Imaging System User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017563_EVOS_M5000_Imaging_System_UG.pdf).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
What laser should I use with the EVOS M5000 Imaging System?
The EVOS M5000 Imaging System does not use lasers for fluorescence imaging. Instead, it utilizes LED light cubes. For more details on compatible dyes and light cubes, please refer to page 136 of the EVOS M5000 Imaging System User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017563_EVOS_M5000_Imaging_System_UG.pdf).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Can I visualize Alexa Fluor 647 dye using the EVOS M5000 Imaging System?
Yes, you can visualize Alexa Fluor 647 dye using the EVOS M5000 Imaging System. The system supports the Cy5 light cube, which is compatible with Alexa Fluor 647 dye. This allows for effective imaging of samples stained with Alexa Fluor 647 dye.
For more details on compatible dyes and light cubes, please refer to page 136 of the EVOS M5000 Imaging System User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017563_EVOS_M5000_Imaging_System_UG.pdf).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Can I upgrade my EVOS M5000 Imaging System (Cat. No. AMF5000)?
Yes, you can add different objectives, wavelength LEDs, vessel holders, OnStage Incubation (temp/humidity/CO2/hypoxic), even image analysis software (Celleste) at any time. If needed, we also offer an upgrade path to the fully automated EVOS M7000 Imaging System as well.
Please contact Technical Support at tech.support@thermofisher.com for more information.
I'm imaging my fluorescently stained samples on EVOS M7000/ EVOS M5000 imaging system and I can't see any color on the displayed image. How do I visualize my images in color?
Toggle image color display provides an option to display images in pseudo-color or in grayscale in the Viewing area. By default, pseudo-color display is on. Click the Image color display button to display the sample pseudo-colored in the default emission color of the selected channels. Click again for grayscale display.
To change the default pseudo-color, you can go to Settings Tab-> Filter cubes and adjust the pseudo-color for a selected channel to the desired color.