EVOS™ M5000 Imaging System - FAQs

View additional product information for EVOS™ M5000 Imaging System - FAQs (AMF5000)

10 product FAQs found

Can I visualize Alexa Fluor 488 dye using the EVOS M5000 Imaging System?

Yes, you can visualize Alexa Fluor 488 dye using the EVOS M5000 Imaging System. The system supports the GFP light cube, which is compatible with Alexa Fluor 488 dye. This allows for effective imaging of samples stained with Alexa Fluor 488 dye.

For more details on compatible dyes and light cubes, please refer to page 136 of the EVOS M5000 Imaging System User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017563_EVOS_M5000_Imaging_System_UG.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What laser should I use with the EVOS M5000 Imaging System?

The EVOS M5000 Imaging System does not use lasers for fluorescence imaging. Instead, it utilizes LED light cubes. For more details on compatible dyes and light cubes, please refer to page 136 of the EVOS M5000 Imaging System User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017563_EVOS_M5000_Imaging_System_UG.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I visualize Alexa Fluor 647 dye using the EVOS M5000 Imaging System?

Yes, you can visualize Alexa Fluor 647 dye using the EVOS M5000 Imaging System. The system supports the Cy5 light cube, which is compatible with Alexa Fluor 647 dye. This allows for effective imaging of samples stained with Alexa Fluor 647 dye.

For more details on compatible dyes and light cubes, please refer to page 136 of the EVOS M5000 Imaging System User Guide (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0017563_EVOS_M5000_Imaging_System_UG.pdf).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I upgrade my EVOS M5000 Imaging System (Cat. No. AMF5000)?

Yes, you can add different objectives, wavelength LEDs, vessel holders, OnStage Incubation (temp/humidity/CO2/hypoxic), even image analysis software (Celleste) at any time. If needed, we also offer an upgrade path to the fully automated EVOS M7000 Imaging System as well.
Please contact Technical Support at tech.support@thermofisher.com for more information.

I'm imaging my fluorescently stained samples on EVOS M7000/ EVOS M5000 imaging system and I can't see any color on the displayed image. How do I visualize my images in color?

Toggle image color display provides an option to display images in pseudo-color or in grayscale in the Viewing area. By default, pseudo-color display is on. Click the Image color display button to display the sample pseudo-colored in the default emission color of the selected channels. Click again for grayscale display.

To change the default pseudo-color, you can go to Settings Tab-> Filter cubes and adjust the pseudo-color for a selected channel to the desired color.

What service options do you offer for the EVOS M5000 Imaging System?

The EVOS M5000 Imaging System comes with a standard 12-month warranty that allows for the unit replacement in case of malfunction. Additional 1-year extended warranty can be purchased separately (contact Technical Support at techsupport@thermofisher.com for details), or alternatively, one can purchase the EVOS M5000 Imaging System Extended Warranty Package (Cat. No. A40486) that will provide the EVOS M5000 Imaging System with the standard 12-month warranty plus 1-year extended warranty.

How should I maintain the EVOS M5000 Imaging System?

For regular maintenance, the EVOS M5000 Imaging System can be turned off, wiped gently with lightly dampened Kimwipes™ tissues, and covered with the dust cover provided with the instrument.

For decontamination, lightly wipe working surfaces of the EVOS M5000 Imaging System (stage top, objective turret, housing, etc.) with paper towels or Kimwipes™ tissues dampened with 70% ethanol or 4,000 ppm hydrogen peroxide (H2O2).

What is the limit of resolution for the EVOS M5000 Imaging System?

At the highest magnification (100X), the field of view is 126 µm x 95 µm with an EVOS 100X objective and 103 µm x 78 µm with an Olympus 100X objective. The internal magnification is 22.8X for EVOS objectives and 27.9X for Olympus objectives.

What applications can I use the EVOS M5000 Imaging System for?

The EVOS M5000 Imaging System is a fully integrated digital-inverted benchtop microscope for four-color fluorescence, transmitted light, and color imaging. Compatible applications include fluorescent cell and tissue staining, fluorescence viability assays, fluorescence reporter assays, apoptosis detection, transduction efficiency, cell counting and confluence, Z-Stack, time-lapse, and phase contrast.

How do the specific parameters on an objective relate to my application and how do I select the right one for my EVOS imaging system?

Please find a comprehensive objective selection guide in the link below. It provides detailed guidance based on sample type, vessel type and mode of imaging.
https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/cell-imaging-systems/evos-objectives/selection-guide-evos-objectives.html