Tyramide Signal Amplification for Immunofluorescent Enhancement.
AuthorsFaget L, Hnasko TS
Journal
PubMed ID26160574
'Enzyme-linked signal amplification is a key technique used to enhance the immunohistochemical detection of protein, mRNA, and other molecular species. Tyramide signal amplification (TSA) is based on a catalytic reporter deposit in close vicinity to the epitope of interest. The advantages of this technique are its simplicity, enhanced sensitivity, high ... More
Activation of the AKT Pathway in the Ascending Aorta With Bicuspid Aortic Valve.
AuthorsHirata Y, Aoki H, Shojima T, Takagi K, Takaseya T, Akasu K, Tobinaga S, Fukumoto Y, Tanaka H,
JournalCirc J
PubMed ID30089758
Dilatation of the ascending aorta affects those patients with bicuspid aortic valve (BAV), even after valvular surgery, possibly due to tissue fragility. The goal of the study was the molecular characterization of aorta with BAV compared to that with normal tricuspid aortic valve (TAV). Methods and Results: The subjects were ... More
Codeficiency of Lysosomal Mucolipins 3 and 1 in Cochlear Hair Cells Diminishes Outer Hair Cell Longevity and Accelerates Age-Related Hearing Loss.
AuthorsWiwatpanit T, Remis NN, Ahmad A, Zhou Y, Clancy JC, Cheatham MA, García-Añoveros J,
JournalJ Neurosci
PubMed ID29453205
Acquired hearing loss is the predominant neurodegenerative condition associated with aging in humans. Although mutations on several genes are known to cause congenital deafness in newborns, few genes have been implicated in age-related hearing loss (ARHL), perhaps because its cause is likely polygenic. Here, we generated mice lacking lysosomal calcium ... More
Transcriptomal signatures of vaccine adjuvants and accessory immunostimulation of sentinel cells by toll-like receptor 2/6 agonists.
AuthorsSalyer ACD, David SA,
JournalHum Vaccin Immunother
PubMed ID29852079
An important component of vaccine development is the identification of safe and effective adjuvants. We sought to identify transcriptomal signatures of innate immune stimulating molecules using next-generation RNA sequencing with the goal of being able to utilize such signatures in identifying novel immunostimulatory compounds with adjuvant activity. The CC family ... More
BMP-IHH-mediated interplay between mesenchymal stem cells and osteoclasts supports calvarial bone homeostasis and repair.
AuthorsGuo Y, Yuan Y, Wu L, Ho TV, Jing J, Sugii H, Li J, Han X, Feng J, Guo C, Chai Y,
JournalBone Res
PubMed ID30345151
Calvarial bones are connected by fibrous sutures. These sutures provide a niche environment that includes mesenchymal stem cells (MSCs), osteoblasts, and osteoclasts, which help maintain calvarial bone homeostasis and repair. Abnormal function of osteogenic cells or diminished MSCs within the cranial suture can lead to skull defects, such as craniosynostosis. Despite the ... More