Bolt™ 抗氧化剂
Bolt™ 抗氧化剂
Invitrogen™

Bolt™ 抗氧化剂

Bolt 抗氧化剂可防止样品再氧化，在蛋白质凝胶电泳及转印过程维持蛋白质的还原状态。在还原性条件下进行蛋白质凝胶电泳时，将其加入电泳缓冲液。Bolt 抗氧化剂可随还原性蛋白质迁移，以维持还原状态并防止敏感氨基酸（如甲硫氨酸和色氨酸）重新氧化。Bolt 抗氧化剂应与 Bolt了解更多信息
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货号数量
BT000515 ml
货号 BT0005
价格（CNY)
434.00
Each
添加至购物车
数量:
15 ml
价格（CNY)
434.00
Each
添加至购物车
Bolt 抗氧化剂可防止样品再氧化，在蛋白质凝胶电泳及转印过程维持蛋白质的还原状态。在还原性条件下进行蛋白质凝胶电泳时，将其加入电泳缓冲液。Bolt 抗氧化剂可随还原性蛋白质迁移，以维持还原状态并防止敏感氨基酸（如甲硫氨酸和色氨酸）重新氧化。

Bolt 抗氧化剂应与 Bolt 样品还原剂或其他还原剂还原的样品配合使用。针对 Bolt 凝胶的中性 pH 体系优化，与其他凝胶化学试剂不兼容。Bolt 抗氧化剂还可与 Bolt 转印缓冲液配合使用，以增强将蛋白转印到膜上的能力。

查看所有 SDS-PAGE 可用的缓冲液和试剂

仅供科研使用。不可用于诊断程序。
规格
缓冲液抗氧化剂
形式液体
数量15 ml
凝胶兼容性Bolt™ Bis-Tris 增强型凝胶
产品线Bolt™
产品类型抗氧化剂
Unit SizeEach
内容与储存
储存于 4°C。

常见问题解答 (FAQ)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What are the storage conditions for Bolt gels?

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the shelf life for Bolt gels?

The shelf life for Bolt gels is similar to that for NuPAGE gels: 12 months from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

View all Bolt™ 抗氧化剂 FAQs