Bolt™ 转印缓冲液 (20X)
Bolt™ 转印缓冲液 (20X)
Invitrogen™

Bolt™ 转印缓冲液 (20X)

Bolt 转印缓冲液 (20X) 用于将蛋白质从 Bolt Bis-Tris Plus 凝胶转印至膜上，以便进行蛋白质免疫印迹分析。其可在电泳过程中维持指定的中性 pH了解更多信息
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货号数量
BT000611L
BT0006125mL
货号 BT00061
价格（CNY)
2,246.00
Each
添加至购物车
数量:
1L
价格（CNY)
2,246.00
Each
添加至购物车
Bolt 转印缓冲液 (20X) 用于将蛋白质从 Bolt Bis-Tris Plus 凝胶转印至膜上，以便进行蛋白质免疫印迹分析。其可在电泳过程中维持指定的中性 pH 环境。中性 pH 条件可保护氨基酸侧链不受改性，并与Edman 降解的 N-末端蛋白测序兼容。

Bolt 抗氧化剂可与 Bolt 转印缓冲液配合使用，以增强将还原蛋白转印到膜上的能力。

查看所有 SDS-PAGE 可用的缓冲液和试剂

仅供科研使用。不可用于诊断程序。
规格
缓冲液转印缓冲液
最大浓度20 X
形式液体
凝胶兼容性Bolt™ Bis-Tris Plus 预制胶
产品线Bolt™
产品类型Western 印迹转印缓冲液
数量1L
容积（公制）1 L
Unit SizeEach
内容与储存
4至 25°C 储存。

常见问题解答 (FAQ)

我的转膜缓冲液的pH值偏离推荐值0.2单位，能否继续使用？

我们建议丢弃缓冲液，并在再次检查试剂和水的纯度后重新配制。不建议用酸或碱调节pH，因为这会使缓冲液的电导率升高，导致转印期间电流过高。

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What are the storage conditions for Bolt gels?

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the shelf life for Bolt gels?

The shelf life for Bolt gels is similar to that for NuPAGE gels: 12 months from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

View all Bolt™ 转印缓冲液 (20X) FAQs