Bolt™ Transfer Buffer (20X), 1L - FAQs

View additional product information for Bolt™ Transfer Buffer (20X) - FAQs (BT00061, BT0006)

10 product FAQs found

我的转膜缓冲液的pH值偏离推荐值0.2单位，能否继续使用？

我们建议丢弃缓冲液，并在再次检查试剂和水的纯度后重新配制。不建议用酸或碱调节pH，因为这会使缓冲液的电导率升高，导致转印期间电流过高。

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

What are the storage conditions for Bolt gels?

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

What is the shelf life for Bolt gels?

The shelf life for Bolt gels is similar to that for NuPAGE gels: 12 months from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.

Find additional tips, troubleshooting help, and resources within our Protein Gel 1D Electrophoresis Support Center.

Which power supplies are compatible with the Mini Gel Tank?

The most common power supplies from Thermo Fisher Scientific, Bio-Rad, and Hoefer are compatible. Also, power supply adapters are available for power supplies not designed for use with covered or non-retractable power leads. Thermo Fisher Owl branded gel tanks are not compatible

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Are Bolt gels compatible with transfer devices from other suppliers?

Yes. While we would prefer that you use our devices, Bolt gels can also be transferred using devices from Bio-Rad, including the Mini Trans-Blot Cell, Trans-Blot SD Semi-Dry Transfer Cell, or Trans-Blot Turbo Transfer System.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Are Bolt gels compatible with other transfer devices?

Yes. You can use the iBlot Dry Transfer System or XCell II Blot Module with SureLock tank and achieve similar transfer efficiency.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The pH of my transfer buffer deviates from the recommended value by 0.2 units. Can I still use the buffer?

We recommend discarding the buffer and remaking it after rechecking the reagents and the water purity. We do not recommend adjusting the pH with acid or base, as this will increase the conductivity of the buffer and result in higher current during the transfer.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.