CellLight™ ER-GFP，BacMam 2.0
CellLight™ ER-GFP，BacMam 2.0
引用和文献 (26)
Invitrogen™

CellLight™ ER-GFP，BacMam 2.0

CellLight™ ER-GFP, BacMam 2.0 可采用绿色荧光蛋白 (GFP) 轻松标记活细胞中的内质网 (ER)。您只需要将试剂加入到细胞中，过夜孵育，第二天早上即可直接进行成像观察。想要标记其他细胞结构？了解更多有关了解更多信息
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货号数量
C105901 瓶
货号 C10590
价格（CNY)
8,491.00
Each
添加至购物车
数量:
1 瓶
价格（CNY)
8,491.00
Each
添加至购物车
CellLight™ ER-GFP, BacMam 2.0 可采用绿色荧光蛋白 (GFP) 轻松标记活细胞中的内质网 (ER)。您只需要将试剂加入到细胞中，过夜孵育，第二天早上即可直接进行成像观察。

想要标记其他细胞结构？了解更多有关 CellLight™ 荧光蛋白标记工具的信息

这种即用型构建体可使用 BacMam 2.0 技术转染到细胞中，并在此表达融合到钙网蛋白内质网信号序列和 KDEL（内质网保留信号）的 GFP，细胞破坏程度极小。您可使用荧光成像观察活细胞中的内质网-GFP 行为，并结合其他荧光蛋白或有机染料进行多重分析。

表达 CellLight™ 构建体的细胞也可采用甲醛固定，以便利用免疫细胞化学技术进行多通路成像。

CellLight™ 技术特性：
•快速便捷：只需将 CellLight™ 试剂加入细胞，过夜孵育，随后即可成像—或冷冻储存，以便后续实验直接使用
• 高效：转导率高达 90%，适用于多种哺乳动物细胞系，包括原代细胞、干细胞和神经元
• 灵活：在多重分析实验或共定位研究中，可共转导多种 BacMam 试剂；通过简单调整剂量即可严格控制表达水平
• 低毒性：CellLight™ 试剂不会在哺乳动物细胞中复制，适于生物安全等级 (BSL) 1 级处理

BacMam 技术
CellLight™ ER-GFP (BacMam 2.0) 是钙网蛋白内质网信号序列和 KDEL（内质网保留信号）与 emGFP 的融合构建体，可准确、特异地靶向细胞内质网-GFP。这种融合构建体包装在昆虫病毒杆状病毒中，不会在人类细胞中复制，生物安全等级 (BSL) 1 级，被指定为可以在大多数实验室中安全使用。BacMam 技术可高效、超低毒性转导/转染大多数哺乳动物细胞类型。这种瞬时转染检测可从过夜孵育后持续最多五天 — 足以完成大部分动态细胞分析。与所有转染/转导技术一样，BacMam 方法无法以同等效率转染/转导所有细胞，因此不太适合细胞群研究或自动成像/计数。CellLight™ 试剂非常适合细胞或亚细胞共定位实验，以及需要特殊分辨率的细胞功能研究。
仅供科研使用。不可用于诊断程序。
规格
颜色绿色
检测方法荧光
染料类型GFP (EmGFP)
发射可见光
激发波长范围488⁄510
适用于（设备）共聚焦显微镜、荧光显微镜
形式液体
产品线CellLight
数量1 瓶
运输条件湿冰
技术荧光强度
标签类型荧光蛋白
产品类型内质网
亚细胞定位内质网
Unit SizeEach
内容与储存
在 2°C 至 6°C 下避光储存。切勿冷冻。

常见问题解答 (FAQ)

我采用CellLight 标记试剂处理神经元时，转导效率很低，该如何提高转染效率？

与许多其它细胞相比，神经元转导更为困难。提高转导效率的主要方法是采用更多数量的病毒颗粒标记细胞。对于原代神经元，在铺盘时转导要比已培养细胞转导效果更好。此外，蛋白的表达在神经元中发生较慢，表达高峰通常发生于2-3天后而非转导后16小时。

How can I increase the transduction efficiency with the BacMam 2.0 reagents such as the the CellLight and Premo products?

Try varying particle-to-cell ratio (PPC), incubation volume, temperature and, cell density (if adherent cells are transduced). For adherent cells, we recommend a confluence of about 70%. Following the PPC, adjusting the volume is the next best parameter to change to optimize protein expression. If that doesn't work, you can also use the BacMam Enhancer Kit (Cat. No. B10107).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Is there any way to preserve the CellLights labeling beyond 5 days?

Cells transduced with the CellLights reagents can be stored frozen for several months after transduction, without loss of expression.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are the CellLights products toxic to cells?

If the viral particles are used at the level we recommend, they are very well tolerated by cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

For how long will the CellLights products label my cells?

The BacMam 2.0 CellLights typically express for 5 days after transduction.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CellLight™ ER-GFP，BacMam 2.0 FAQs

引用和文献 (26)

引用和文献
Abstract
Mutations in ABCD4 cause a new inborn error of vitamin B12 metabolism.
Authors:Coelho D, Kim JC, Miousse IR, Fung S, du Moulin M, Buers I, Suormala T, Burda P, Frapolli M, Stucki M, Nürnberg P, Thiele H, Robenek H, Höhne W, Longo N, Pasquali M, Mengel E, Watkins D, Shoubridge EA, Majewski J, Rosenblatt DS, Fowler B, Rutsch F, Baumgartner MR,
Journal:Nat Genet
PubMed ID:22922874
Inherited disorders of vitamin B12 (cobalamin) have provided important clues to how this vitamin, which is essential for hematological and neurological function, is transported and metabolized. We describe a new disease that results in failure to release vitamin B12 from lysosomes, which mimics the cblF defect caused by LMBRD1 mutations. ... More
Intracellular trafficking of Clostridium perfringens iota-toxin b.
Authors:Nagahama M, Umezaki M, Tashiro R, Oda M, Kobayashi K, Shibutani M, Takagishi T, Ishidoh K, Fukuda M, Sakurai J,
Journal:Infect Immun
PubMed ID:22825447
'Clostridium perfringens iota-toxin is composed of an enzymatic component (Ia) and a binding component (Ib). Ib binds to a cell surface receptor, undergoes oligomerization in lipid rafts, and binds Ia. The resulting complex is then endocytosed. Here, we show the intracellular trafficking of iota-toxin. After the binding of the Ib ... More
Single-cell redox imaging demonstrates a distinctive response of dopaminergic neurons to oxidative insults.
Authors:Horowitz MP, Milanese C, Di Maio R, Hu X, Montero LM, Sanders LH, Tapias V, Sepe S, van Cappellen WA, Burton EA, Greenamyre JT, Mastroberardino PG,
Journal:Antioxid Redox Signal
PubMed ID:21395478
'The study of the intracellular oxido-reductive (redox) state is of extreme relevance to the dopamine (DA) neurons of the substantia nigra pars compacta. These cells possess a distinct physiology intrinsically associated with elevated reactive oxygen species production, and they selectively degenerate in Parkinson''s disease under oxidative stress conditions. To test ... More
Intracellular trafficking mechanism, from intracellular uptake to extracellular efflux, for phospholipid/cholesterol liposomes.
Authors:Un K, Sakai-Kato K, Oshima Y, Kawanishi T, Okuda H,
Journal:Biomaterials
PubMed ID:22858002
'Liposomes are widely used as drug delivery vehicles to transfer chemotherapeutic agents, proteins, and nucleic acids into target cells. To improve therapeutic effects and reduce unexpected toxic side-effects, it is necessary to understand the mechanism of liposomal uptake into cells, and the intracellular fate of internalized liposomes. The intracellular fate ... More
Der p2 Internalization by Epithelium Synergistically Augments Toll-like Receptor-Mediated Proinflammatory Signaling.
Authors:Yin SC, Liao EC, Chiu CL, Chang CY, Tsai JJ,
Journal:
PubMed ID:25749775
'House-dust-mite (HDM) major allergen Der p2 shares homology and function with Toll-like receptor (TLR) signaling protein myeloid differentiation-2 (MD2) and may lead to airway inflammation. Should Der p2 be internalized by human airway epithelium, it has the theoretical propensity to potentiate epithelium activation. This study aimed to demonstrate the internalization ... More
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