Mutations in ABCD4 cause a new inborn error of vitamin B12 metabolism.
AuthorsCoelho D, Kim JC, Miousse IR, Fung S, du Moulin M, Buers I, Suormala T, Burda P, Frapolli M, Stucki M, Nürnberg P, Thiele H, Robenek H, Höhne W, Longo N, Pasquali M, Mengel E, Watkins D, Shoubridge EA, Majewski J, Rosenblatt DS, Fowler B, Rutsch F, Baumgartner MR,
JournalNat Genet
PubMed ID22922874
Inherited disorders of vitamin B12 (cobalamin) have provided important clues to how this vitamin, which is essential for hematological and neurological function, is transported and metabolized. We describe a new disease that results in failure to release vitamin B12 from lysosomes, which mimics the cblF defect caused by LMBRD1 mutations. ... More
Intracellular trafficking of Clostridium perfringens iota-toxin b.
AuthorsNagahama M, Umezaki M, Tashiro R, Oda M, Kobayashi K, Shibutani M, Takagishi T, Ishidoh K, Fukuda M, Sakurai J,
JournalInfect Immun
PubMed ID22825447
'Clostridium perfringens iota-toxin is composed of an enzymatic component (Ia) and a binding component (Ib). Ib binds to a cell surface receptor, undergoes oligomerization in lipid rafts, and binds Ia. The resulting complex is then endocytosed. Here, we show the intracellular trafficking of iota-toxin. After the binding of the Ib ... More
Single-cell redox imaging demonstrates a distinctive response of dopaminergic neurons to oxidative insults.
AuthorsHorowitz MP, Milanese C, Di Maio R, Hu X, Montero LM, Sanders LH, Tapias V, Sepe S, van Cappellen WA, Burton EA, Greenamyre JT, Mastroberardino PG,
JournalAntioxid Redox Signal
PubMed ID21395478
'The study of the intracellular oxido-reductive (redox) state is of extreme relevance to the dopamine (DA) neurons of the substantia nigra pars compacta. These cells possess a distinct physiology intrinsically associated with elevated reactive oxygen species production, and they selectively degenerate in Parkinson''s disease under oxidative stress conditions. To test ... More
Intracellular trafficking mechanism, from intracellular uptake to extracellular efflux, for phospholipid/cholesterol liposomes.
AuthorsUn K, Sakai-Kato K, Oshima Y, Kawanishi T, Okuda H,
JournalBiomaterials
PubMed ID22858002
'Liposomes are widely used as drug delivery vehicles to transfer chemotherapeutic agents, proteins, and nucleic acids into target cells. To improve therapeutic effects and reduce unexpected toxic side-effects, it is necessary to understand the mechanism of liposomal uptake into cells, and the intracellular fate of internalized liposomes. The intracellular fate ... More
Der p2 Internalization by Epithelium Synergistically Augments Toll-like Receptor-Mediated Proinflammatory Signaling.
'House-dust-mite (HDM) major allergen Der p2 shares homology and function with Toll-like receptor (TLR) signaling protein myeloid differentiation-2 (MD2) and may lead to airway inflammation. Should Der p2 be internalized by human airway epithelium, it has the theoretical propensity to potentiate epithelium activation. This study aimed to demonstrate the internalization ... More
Location and the functionality of erythropoietin receptor(s) in A2780 cells.
AuthorsSolár P, Hrcková G, Varinská L, Solárová Z, Kriška J, Uhrínová I, Kello M, Mojžiš J, Fedorocko P, Sytkowski AJ,
JournalOncol Rep
PubMed ID22552716
'Erythropoietin (Epo) is a critical regulator of erythroid cell proliferation, differentiation and apoptosis. In the form of a recombinant protein, it is widely used to treat various forms of anemia, including that associated with cancer and with the myelosuppressive effects of chemotherapy. Studies of ovarian cancer cell lines have demonstrated ... More
A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part III: Reagents for Actin, Tubulin, Cellular Membranes, and Whole Cell and Cytoplasm.
AuthorsKilgore JA, Dolman NJ, Davidson MW,
Journal
PubMed ID24510770
'Non-antibody commercial fluorescent reagents for imaging of cytoskeletal structures have been limited primarily to tubulin and actin, with the main factor in choice based mainly on whether cells are live or fixed and permeabilized. A wider range of options exist for cell membrane dyes, and the choice of reagent primarily ... More
Live cell imaging of G protein-coupled receptors.
AuthorsTeichmann A, Schmidt A, Wiesner B, Oksche A, Schülein R,
JournalMethods Mol Biol
PubMed ID22674164
Live cell imaging experiments with G protein-coupled receptors (GPCRs) tagged with fluorescent fusion proteins were originally performed to study trafficking and subcellular location of these important drug targets. In the past decade, however, substantial progress came from improved imaging methods and from the cloning of novel fluorescent fusion proteins. Today, ... More
Drop in endo/sarcoplasmic calcium precedes the unfolded protein response in Brefeldin A-treated vascular smooth muscle cells.
AuthorsZiomek G, van Breemen C, Esfandiarei M,
Journal
PubMed ID26172080
The present study addresses the causal relationship between induction of endo/sarcoplasmic reticulum stress and dysregulation of calcium transport, while examining whether the most widely-used experimental endo/sarcoplasmic reticulum stressors can be considered appropriate for elucidating underlying cellular mechanisms involved during the progression of the unfolded protein response in vascular smooth muscle ... More
The abcEDCBA-Encoded ABC Transporter and the virB Operon-Encoded Type IV Secretion System of Brucella ovis Are Critical for Intracellular Trafficking and Survival in Ovine Monocyte-Derived Macrophages.
AuthorsMacedo AA, Silva AP, Mol JP, Costa LF, Garcia LN, Araújo MS, Martins Filho OA, Paixão TA, Santos RL,
Journal
PubMed ID26366863
Brucella ovis infection is associated with epididymitis, orchitis and infertility in rams. Most of the information available on B. ovis and host cell interaction has been generated using murine macrophages or epithelial cell lines, but the interaction between B. ovis and primary ovine macrophages has not been studied. The aim ... More
The amyloid precursor protein copper binding domain histidine residues 149 and 151 mediate APP stability and metabolism.
One of the key pathological hallmarks of Alzheimer disease (AD) is the accumulation of the APP-derived amyloid ß peptide (Aß) in the brain. Altered copper homeostasis has also been reported in AD patients and is thought to increase oxidative stress and to contribute to toxic Aß accumulation and regulate APP ... More
Polyplexes traffic through caveolae to the Golgi and endoplasmic reticulum en route to the nucleus.
AuthorsReilly MJ, Larsen JD, Sullivan MO,
JournalMol Pharm
PubMed ID22420286
The cellular machinery involved in the internalization of nonviral gene carriers and their subsequent trafficking to the nucleus directly impacts their therapeutic efficiency. Hence, identifying key endocytic pathways and organelles that contribute to the successful transfer of polyplexes to the nucleus generates new opportunities for improving carrier design. Previously, we ... More
A novel pathogenic role of the ER chaperone GRP78/BiP in rheumatoid arthritis.
AuthorsYoo SA, You S, Yoon HJ, Kim DH, Kim HS, Lee K, Ahn JH, Hwang D, Lee AS, Kim KJ, Park YJ, Cho CS, Kim WU,
JournalJ Exp Med
PubMed ID22430489
An accumulation of misfolded proteins can trigger a cellular survival response in the endoplasmic reticulum (ER). In this study, we found that ER stress-associated gene signatures were highly expressed in rheumatoid arthritis (RA) synoviums and synovial cells. Proinflammatory cytokines, such as TNF and IL-1ß, increased the expression of GRP78/BiP, a ... More
High-efficiency transient transduction of human embryonic stem cell-derived neurons with baculoviral vectors.
AuthorsZeng J, Du J, Lin J, Bak XY, Wu C, Wang S,
JournalMol Ther
PubMed ID19532141
Transient genetic manipulation of human neurons without chromosomal integration of the transgene would be valuable but has been challenging due to the quiescent nature of these postmitotic cells. In this study, we developed a set of baculoviral vectors for transient transduction in nondividing neurons derived from human embryonic stem cells ... More
Baculovirus-mediated gene transfer into mammalian cells.
AuthorsBoyce FM, Bucher NL,
JournalProc Natl Acad Sci U S A
PubMed ID8637876
This paper describes the use of the baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) as a vector for gene delivery into mammalian cells. A modified AcMNPV virus was prepared that carried the Escherichia coli lacZ reporter gene under control of the Rous sarcoma virus promoter and mammalian RNA processing ... More
BacMam technology and its application to drug discovery.
AuthorsAmes RS, Kost TA, Condreay JP,
JournalExpert Opin Drug Discov
PubMed ID23488908
The recombinant baculovirus/insect cell system was firmly established as a leading method for recombinant protein production when a new potential use for these viruses was revealed in 1995. It was reported that engineered recombinant baculoviruses could deliver functional expression cassettes to mammalian cell types; a system which has come to ... More
Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes.
Authors
JournalCell Death Differ
PubMed ID34999730
Mannose glycosylation is an integral step for NIS localization and function in human breast cancer cells.
Authors
JournalJ Cell Sci
PubMed ID31455607
Biophysical comparison of four silver nanoparticles coatings using microscopy, hyperspectral imaging and flow cytometry.
Authors
JournalPLoS One
PubMed ID31365549
Nanoparticles restore lysosomal acidification defects: Implications for Parkinson and other lysosomal-related diseases.
Authors
JournalAutophagy
PubMed ID26761717
Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.
Authors
JournalJ Biol Chem
PubMed ID30190326
Axonal generation of amyloid-β from palmitoylated APP in mitochondria-associated endoplasmic reticulum membranes.
Authors
JournalCell Rep
PubMed ID34010653
Cycloheximide promotes paraptosis induced by inhibition of cyclophilins in glioblastoma multiforme.
Authors
JournalCell Death Dis
PubMed ID28518150
Dysregulation of mitochondria-lysosome contacts by GBA1 dysfunction in dopaminergic neuronal models of Parkinson's disease.
Authors
JournalNat Commun
PubMed ID33753743
Transit and integration of extracellular mitochondria in human heart cells.
Authors
JournalSci Rep
PubMed ID29234096
Spatiotemporally controlled induction of autophagy-mediated lysosome turnover.