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引用和文献 (21)
Invitrogen™
羧基-H2DFFDA
H2DFFDA 是一种化学还原型、乙酰化形式的荧光素，用作细胞中活性氧簇 (ROS) 的指示剂。这种非荧光分子在细胞内容易被酯酶分解和 ROS 氧化，使乙酸酯基团脱去，转化为绿色荧光形式。与氯化荧光素衍生物（DCF了解更多信息
| 货号 | 数量 |
|---|---|
| C13293 | 5 mg |
货号 C13293
价格（CNY)
4,661.00
Each
数量:
5 mg
价格（CNY)
4,661.00
Each
H2DFFDA 是一种化学还原型、乙酰化形式的荧光素，用作细胞中活性氧簇 (ROS) 的指示剂。这种非荧光分子在细胞内容易被酯酶分解和 ROS 氧化，使乙酸酯基团脱去，转化为绿色荧光形式。与氯化荧光素衍生物（DCF、DCFDA）相比，该氟化类似物显示出更好的光稳定性。
ROS 指示剂质量标准：
• Ex/Em：∼492–495/517–527 nm
• 产品对空气敏感，应储存在干燥的氩气或氮气中
• 产品可溶于 DMSO、DMF 或乙醇中使用
• 指示剂可渗透细胞（细胞上样方案见文献）
• 可使用流式细胞仪、荧光计、微孔板读数仪或荧光显微镜（配有适用于荧光素的激发光源和滤光片）对荧光信号进行监测
查找更多 ROS 指示剂
我们提供了一系列 Molecular Probes™ 产品，用于生成活性氧簇 (ROS)（包括单线态氧、超氧化物、羟基自由基、多种过氧化物和过氧化氢）以及用于在溶液中进行相应荧光检测。有关这些产品的更多信息，请查阅 Molecular Probes™ 手册第 18.2 节—生成和检测活性氧。
供研究使用。不可用于人或动物的治疗或诊断。
ROS 指示剂质量标准：
• Ex/Em：∼492–495/517–527 nm
• 产品对空气敏感，应储存在干燥的氩气或氮气中
• 产品可溶于 DMSO、DMF 或乙醇中使用
• 指示剂可渗透细胞（细胞上样方案见文献）
• 可使用流式细胞仪、荧光计、微孔板读数仪或荧光显微镜（配有适用于荧光素的激发光源和滤光片）对荧光信号进行监测
查找更多 ROS 指示剂
我们提供了一系列 Molecular Probes™ 产品，用于生成活性氧簇 (ROS)（包括单线态氧、超氧化物、羟基自由基、多种过氧化物和过氧化氢）以及用于在溶液中进行相应荧光检测。有关这些产品的更多信息，请查阅 Molecular Probes™ 手册第 18.2 节—生成和检测活性氧。
供研究使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
数量5 mg
产品类型ROS Indicator
Unit SizeEach
引用和文献 (21)
引用和文献
Abstract
SuperTAG methylation-specific digital karyotyping reveals uremia-induced epigenetic dysregulation of atherosclerosis-related genes.
Journal:Circ Cardiovasc Genet
PubMed ID:23074332
'Accelerated atherosclerosis is a hallmark of chronic kidney disease (CKD). Although the role of epigenetic dysregulation in atherosclerosis is increasingly appreciated, only a few studies focused on epigenetics in CKD-associated cardiovascular disease, virtually all of which assessed epigenetic dysregulation globally. We hypothesized that gene-specific epigenetic dysregulation in CKD exists, affecting
Distinct immunologic effects of different intravenous iron preparations on monocytes.
Journal:
PubMed ID:24523357
'Iron deficiency contributes to anaemia in patients with chronic kidney disease. I.v. iron is therefore widely used for anaemia treatment, although it may induce oxidative stress and activate monocytes. Different i.v. iron preparations are available, but interestingly their substance-specific immunologic effects are poorly studied. We analysed the effect of iron
Cell transformation by FLT3 ITD in acute myeloid leukemia involves oxidative inactivation of the tumor suppressor protein-tyrosine phosphatase DEP-1/ PTPRJ.
Journal:Blood
PubMed ID:22438257
'Signal transduction of FMS-like tyrosine kinase 3 (FLT3) is regulated by protein-tyrosine phosphatases (PTPs). We recently identified the PTP DEP-1/CD148/PTPRJ as a novel negative regulator of FLT3. This study addressed the role of DEP-1 for regulation of the acute myeloid leukemia (AML)-related mutant FLT3 internal tandem duplication (ITD) protein. Our
Cystine dimethylester loading promotes oxidative stress and a reduction in ATP independent of lysosomal cystine accumulation in a human proximal tubular epithelial cell line.
Journal:
PubMed ID:23813804
'Using the cystine dimethylester (CDME) loading technique to achieve elevated lysosomal cystine levels, ATP depletion has previously been postulated to be responsible for the renal dysfunction in cystinosis, a genetic disorder characterized by an excessive accumulation of cystine in the lysosomes. However, this is unlikely to be the sole factor
Platelets and platelet-like particles mediate intercellular RNA transfer.
Journal:Blood
PubMed ID:22596260
The role of platelets in hemostasis and thrombosis is clearly established; however, the mechanisms by which platelets mediate inflammatory and immune pathways are less well understood. Platelets interact and modulate the function of blood and vascular cells by releasing bioactive molecules. Although the platelet is anucleate, it contains transcripts that