SuperTAG methylation-specific digital karyotyping reveals uremia-induced epigenetic dysregulation of atherosclerosis-related genes.
AuthorsZawada AM, Rogacev KS, Hummel B, Grün OS, Friedrich A, Rotter B, Winter P, Geisel J, Fliser D, Heine GH,
JournalCirc Cardiovasc Genet
PubMed ID23074332
'Accelerated atherosclerosis is a hallmark of chronic kidney disease (CKD). Although the role of epigenetic dysregulation in atherosclerosis is increasingly appreciated, only a few studies focused on epigenetics in CKD-associated cardiovascular disease, virtually all of which assessed epigenetic dysregulation globally. We hypothesized that gene-specific epigenetic dysregulation in CKD exists, affecting ... More
Distinct immunologic effects of different intravenous iron preparations on monocytes.
'Iron deficiency contributes to anaemia in patients with chronic kidney disease. I.v. iron is therefore widely used for anaemia treatment, although it may induce oxidative stress and activate monocytes. Different i.v. iron preparations are available, but interestingly their substance-specific immunologic effects are poorly studied. We analysed the effect of iron ... More
Cell transformation by FLT3 ITD in acute myeloid leukemia involves oxidative inactivation of the tumor suppressor protein-tyrosine phosphatase DEP-1/ PTPRJ.
AuthorsGodfrey R, Arora D, Bauer R, Stopp S, Müller JP, Heinrich T, Böhmer SA, Dagnell M, Schnetzke U, Scholl S, Östman A, Böhmer FD,
JournalBlood
PubMed ID22438257
'Signal transduction of FMS-like tyrosine kinase 3 (FLT3) is regulated by protein-tyrosine phosphatases (PTPs). We recently identified the PTP DEP-1/CD148/PTPRJ as a novel negative regulator of FLT3. This study addressed the role of DEP-1 for regulation of the acute myeloid leukemia (AML)-related mutant FLT3 internal tandem duplication (ITD) protein. Our ... More
Cystine dimethylester loading promotes oxidative stress and a reduction in ATP independent of lysosomal cystine accumulation in a human proximal tubular epithelial cell line.
AuthorsSumayao R, McEvoy B, Martin-Martin N, McMorrow T, Newsholme P,
Journal
PubMed ID23813804
'Using the cystine dimethylester (CDME) loading technique to achieve elevated lysosomal cystine levels, ATP depletion has previously been postulated to be responsible for the renal dysfunction in cystinosis, a genetic disorder characterized by an excessive accumulation of cystine in the lysosomes. However, this is unlikely to be the sole factor ... More
Platelets and platelet-like particles mediate intercellular RNA transfer.
AuthorsRisitano A, Beaulieu LM, Vitseva O, Freedman JE,
JournalBlood
PubMed ID22596260
The role of platelets in hemostasis and thrombosis is clearly established; however, the mechanisms by which platelets mediate inflammatory and immune pathways are less well understood. Platelets interact and modulate the function of blood and vascular cells by releasing bioactive molecules. Although the platelet is anucleate, it contains transcripts that ... More
SuperSAGE evidence for CD14++CD16+ monocytes as a third monocyte subset.
Monocytes are a heterogeneous cell population with subset-specific functions and phenotypes. The differential expression of CD14 and CD16 distinguishes classical CD14(++)CD16(-), intermediate CD14(++)CD16(+), and nonclassical CD14(+)CD16(++) monocytes. Current knowledge on human monocyte heterogeneity is still incomplete: while it is increasingly acknowledged that CD14(++)CD16(+) monocytes are of outstanding significance in 2 ... More
Triamcinolone acetonide-mediated oxidative injury in retinal cell culture: comparison with dexamethasone.
AuthorsChung H, Hwang JJ, Koh JY, Kim JG, Yoon YH,
JournalInvest Ophthalmol Vis Sci
PubMed ID18055827
To investigate the cytotoxicity of triamcinolone acetonide (TA) and dexamethasone (DXM) in various types of cells in retinal cell culture. Primary rat retinal cell cultures were treated with 25 to 800 microg/mL TA (58 microM-1.8 mM) or DXM (48 microM-1.6 mM) for 12 to 24 hours. Cell survival and death ... More
High efficacy of a Listeria-based vaccine against metastatic breast cancer reveals a dual mode of action.
AuthorsKim SH, Castro F, Paterson Y, Gravekamp C,
JournalCancer Res
PubMed ID19584282
Most cancer vaccines induce CTL responses to tumor-associated antigens (TAA). Killing of tumor cells occurs through TAA-specific CTL-mediated cytolysis. Here, we show that one preventive followed by two therapeutic immunizations with an attenuated Listeria monocytogenes (LM)-based vaccine eradicates all metastases and almost the entire primary tumor in the syngeneic, aggressive ... More
The omega-3 polyunsaturated fatty acid docosahexaenoic acid inhibits proliferation and progression of non-small cell lung cancer cells through the reactive oxygen species-mediated inactivation of the PI3K /Akt pathway.
AuthorsYin Y, Sui C, Meng F, Ma P, Jiang Y
JournalLipids Health Dis
PubMed ID28468627
'Docosahexaenoic acid(DHA) inhibits tumor growth and progression in various cancers, including lung cancer. However, the mechanisms involved remain unclear. The aim of this study was to identify the mechanism of DHA in inhibiting progression of non-small cell lung cancer (NSCLC) in vitro.' ... More
GRK5 is an essential co-repressor of the cardiac mineralocorticoid receptor and is selectively induced by finerenone.
Authors
JournalWorld J Cardiol
PubMed ID35582468
Inhibiting histone deacetylase 6 partly protects cultured rat cortical neurons from oxygen‑glucose deprivation‑induced necroptosis.
Authors
JournalMol Med Rep
PubMed ID25976407
DNA-Aptamer Raised against Receptor for Advanced Glycation End Products Improves Survival Rate in Septic Mice.
Authors
JournalOxid Med Cell Longev
PubMed ID34413930
Antagonistic Roles of GRK2 and GRK5 in Cardiac Aldosterone Signaling Reveal GRK5-Mediated Cardioprotection via Mineralocorticoid Receptor Inhibition.
Authors
JournalInt J Mol Sci
PubMed ID32326036
20-HETE increases superoxide production and activates NAPDH oxidase in pulmonary artery endothelial cells.
Authors
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID18296498
Protein disulphide isomerase as a target for nanoparticle-mediated sensitisation of cancer cells to radiation.
AuthorsTaggart LE, McMahon SJ, Butterworth KT, Currell FJ, Schettino G, Prise KM
JournalNanotechnology
PubMed ID27080849
Radiation resistance and toxicity in normal tissues are limiting factors in the efficacy of radiotherapy. Gold nanoparticles (GNPs) have been shown to be effective at enhancing radiation-induced cell death, and were initially proposed to physically enhance the radiation dose deposited. However, biological responses of GNP radiosensitization based on physical assumptions ... More
Anemone rivularis inhibits pyruvate dehydrogenase kinase activity and tumor growth.
AuthorsChung TW, Lee JH, Choi HJ, Park MJ, Kim EY, Han JH, Jang SB, Lee SO, Lee SW, Hang J, Yi LW, Ha KT
JournalJ Ethnopharmacol
PubMed ID28341246
Anemone rivularis Buch.-Ham. ex DC. (Ranunculaceae) have been used as a traditional remedy for treatment of inflammation and cancer. However, there is no report demonstrating experimental evidence on anti-tumor action of A. rivularis. ... More
Overcome the limitation of hypoxia against photodynamic therapy to treat cancer cells by using perfluorocarbon nanodroplet for photosensitizer delivery.
AuthorsTang X, Cheng Y, Huang S, Zhi F, Yuan A, Hu Y, Wu J
JournalBiochem Biophys Res Commun
PubMed ID28359763
The low oxygen concentration limits the therapeutic efficacy of photodynamic therapy in treating cancer cells in hypoxia, since the cytotoxic
Antiproliferation effect of the uremic toxin para-cresol on endothelial progenitor cells is related to its antioxidant activity.
AuthorsPan L, Ye X, Ding J, Zhou Y
JournalMol Med Rep
PubMed ID28260040
Endothelial dysfunction and impaired endothelial regenerative capacity are key contributors to the high incidence of cardiovascular disease in patients with chronic kidney disease (CKD). Uremic toxins are associated with this pathogenesis. Previous studies have revealed that a uremic toxin, para-cresol (p-cresol), exerts an antiproliferation effect on human endothelial progenitor cells ... More
Anticancer activity study of A
AuthorsMarucci G, Santinelli C, Buccioni M, Navia AM, Lambertucci C, Zhurina A, Yli-Harja O, Volpini R, Kandhavelu M
JournalLife Sci
PubMed ID29763615
A
Gradient sperm selection for reproductive techniques in cattle: Is Isolate a suitable replacement for Percoll?
In assisted reproductive techniques, it is essential to perform a sperm selection to obtain spermatozoa with high motility and membrane integrity for in vitro fertilisation (IVF) and high-DNA integrity for intracytoplasmic sperm injection (ICSI). In this study, we evaluated whether Isolate ... More
In vitro anti-glioblastoma activity of L-valine derived boroxazolidones.
AuthorsViswanathan A, Sebastianelli G, Brown K, Raunio J, Sipilä V, Yli-Harja O, Candeias NR, Kandhavelu M
JournalEur J Pharmacol
PubMed ID30981767
In the present study, a series of L-valine derived boroxazolidones, previously synthesized and reported to have residual activity in a human epithelial cell line, have been evaluated in vitro for their anti-glioblastoma activity. A boroxazolidone derivative containing 2,4-difluorophenyl moieties (6) was found to have higher cytotoxicity than the standard drug, ... More