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引用和文献 (7)
Invitrogen™
Click-IT™ ManNAz 代谢糖蛋白标记试剂（四乙酰化 N-叠氮基乙酰基-D-甘露糖胺）
Click-iT™ ManNAz 代谢糖蛋白标记试剂提供了一种简单而可靠的两步法技术的第一部分，可用于鉴定和表征细胞表面含唾液酸的糖蛋白。在第一步中，使用叠氮化物修饰的甘露糖胺 (ManNAz) 与培养的细胞共同孵育。叠氮基糖经过代谢后通过寡糖生物合成途径的允许性质掺入细胞表面含唾液酸的糖蛋白中。在第二步中，通过叠氮化物和炔烃之间的化学选择性连接或 click 反应，使用了解更多信息
| 货号 | 数量 |
|---|---|
| C33366 | 5.2 mg |
货号 C33366
价格（CNY)
5,333.00
Each
数量:
5.2 mg
价格（CNY)
5,333.00
Each
Click-iT™ ManNAz 代谢糖蛋白标记试剂提供了一种简单而可靠的两步法技术的第一部分，可用于鉴定和表征细胞表面含唾液酸的糖蛋白。在第一步中，使用叠氮化物修饰的甘露糖胺 (ManNAz) 与培养的细胞共同孵育。叠氮基糖经过代谢后通过寡糖生物合成途径的允许性质掺入细胞表面含唾液酸的糖蛋白中。在第二步中，通过叠氮化物和炔烃之间的化学选择性连接或 click 反应，使用 Click-iT™ 糖蛋白检测试剂盒检测凝胶（TAMRA 或 Dapoxyl™ 炔烃）或蛋白质印迹（生物素炔烃）的叠氮基标记糖蛋白。这些 Click-iT™ 产品与 LC-MS⁄MS 及 Multiplexed Proteomics™ 技术兼容，可用于糖蛋白组学的深度分析。
仅供科研使用。不可用于诊断程序。
规格
检测方法基于生物素，荧光
环保功能Less hazardous
产品线Click-iT
产品类型标记试剂
数量5.2 mg
运输条件室温
标记目标蛋白质
标签或染料Alexa Fluor 488, Alexa Fluor 555, Alexa Fluor 594, Alexa Fluor 647, 生物素, Oregon Green 488, TMR（四甲基罗丹明）
Unit SizeEach
内容与储存
在冷冻冰箱（-5°C 至 -30°C）中储存。
引用和文献 (7)
引用和文献
Abstract
Metabolic labeling of glycans with azido sugars for visualization and glycoproteomics.
Journal:Methods Enzymol
PubMed ID:17116478
'The staggering complexity of glycans renders their analysis extraordinarily difficult, particularly in living systems. A recently developed technology, termed metabolic oligosaccharide engineering, enables glycan labeling with probes for visualization in cells and living animals, and enrichment of specific glycoconjugate types for proteomic analysis. This technology involves metabolic labeling of glycans
Copper-free click chemistry for highly luminescent quantum dot conjugates: application to in vivo metabolic imaging.
Journal:Bioconjug Chem
PubMed ID:20222737
Quantum dots (QD) are inorganic nanocrystals with outstanding optical properties, specially suited for biological imaging applications. Their attachment to biomolecules in mild aqueous conditions for the design of bioconjugates is therefore highly desirable. 1,3-dipolar [3 + 2] cycloaddition between azides and terminal alkynes (
Dynamic monitoring of newly synthesized proteomes: up-regulation of myristoylated protein kinase A during butyric acid induced apoptosis.
Journal:Angew Chem Int Ed Engl
PubMed ID:21678537
Doubly charged: A double metabolic incorporation approach capable of proteome-wide profiling of post-translational modification dynamics on newly synthesized proteins has been developed (see scheme; blue box: methionine surrogate, orange diamond: PTM probe). This strategy reveals for the first time that up-regulation of myristoylated PKA protein is necessary for the occurrence
Sialic Acids Attached to O-Glycans Modulate Voltage-gated Potassium Channel Gating.
Journal:J Biol Chem
PubMed ID:21115483
Neuronal, cardiac, and skeletal muscle action potentials are produced and conducted through the highly regulated activity of several types of voltage-gated ion channels. Voltage-gated potassium (K(v)) channels are responsible for action potential repolarization. Glycans can be attached to glycoproteins through N- and O-linkages. Previous reports described the impact of N-glycans
A FRET-based fluorogenic phosphine for live-cell imaging with the Staudinger ligation.
Journal:Angew Chem Int Ed Engl
PubMed ID:18306205
Fluorescent phosphine probes have been used for direct imaging of various azide-bearing biomolecules with the Staudinger ligation in cell-free environments. Recently, we applied phosphine-based dyes to image azides on the surface of live cells. Notably, significant labeling above background could only be achieved using a highly negatively charged