Metabolic labeling of glycans with azido sugars for visualization and glycoproteomics.
AuthorsLaughlin ST, Agard NJ, Baskin JM, Carrico IS, Chang PV, Ganguli AS, Hangauer MJ, Lo A, Prescher JA, Bertozzi CR,
JournalMethods Enzymol
PubMed ID17116478
'The staggering complexity of glycans renders their analysis extraordinarily difficult, particularly in living systems. A recently developed technology, termed metabolic oligosaccharide engineering, enables glycan labeling with probes for visualization in cells and living animals, and enrichment of specific glycoconjugate types for proteomic analysis. This technology involves metabolic labeling of glycans ... More
Copper-free click chemistry for highly luminescent quantum dot conjugates: application to in vivo metabolic imaging.
AuthorsBernardin A, Cazet A, Guyon L, Delannoy P, Vinet F, Bonnaffé D, Texier I,
JournalBioconjug Chem
PubMed ID20222737
Quantum dots (QD) are inorganic nanocrystals with outstanding optical properties, specially suited for biological imaging applications. Their attachment to biomolecules in mild aqueous conditions for the design of bioconjugates is therefore highly desirable. 1,3-dipolar [3 + 2] cycloaddition between azides and terminal alkynes ( ... More
Dynamic monitoring of newly synthesized proteomes: up-regulation of myristoylated protein kinase A during butyric acid induced apoptosis.
AuthorsLiu K, Yang PY, Na Z, Yao SQ,
JournalAngew Chem Int Ed Engl
PubMed ID21678537
Doubly charged: A double metabolic incorporation approach capable of proteome-wide profiling of post-translational modification dynamics on newly synthesized proteins has been developed (see scheme; blue box: methionine surrogate, orange diamond: PTM probe). This strategy reveals for the first time that up-regulation of myristoylated PKA protein is necessary for the occurrence ... More
Sialic Acids Attached to O-Glycans Modulate Voltage-gated Potassium Channel Gating.
AuthorsSchwetz TA, Norring SA, Ednie AR, Bennett ES,
JournalJ Biol Chem
PubMed ID21115483
Neuronal, cardiac, and skeletal muscle action potentials are produced and conducted through the highly regulated activity of several types of voltage-gated ion channels. Voltage-gated potassium (K(v)) channels are responsible for action potential repolarization. Glycans can be attached to glycoproteins through N- and O-linkages. Previous reports described the impact of N-glycans ... More
A FRET-based fluorogenic phosphine for live-cell imaging with the Staudinger ligation.
AuthorsHangauer MJ, Bertozzi CR,
JournalAngew Chem Int Ed Engl
PubMed ID18306205
Fluorescent phosphine probes have been used for direct imaging of various azide-bearing biomolecules with the Staudinger ligation in cell-free environments. Recently, we applied phosphine-based dyes to image azides on the surface of live cells. Notably, significant labeling above background could only be achieved using a highly negatively charged ... More
O-fucosylation of DLL3 is required for its function during somitogenesis.
AuthorsSerth K, Schuster-Gossler K, Kremmer E, Hansen B, Marohn-Köhn B, Gossler A
Journal
PubMed ID25856312
Delta-like 3 (DLL3) is a member of the DSL family of Notch ligands in amniotes. In contrast to DLL1 and DLL4, the other Delta-like proteins in the mouse, DLL3 does not bind in trans to Notch and does not activate the receptor, but shows cis-interaction and cis-inhibitory properties on Notch ... More
BACE2 distribution in major brain cell types and identification of novel substrates.
AuthorsVoytyuk I, Mueller SA, Herber J, Snellinx A, Moechars D, van Loo G, Lichtenthaler SF, De Strooper B
JournalLife Sci Alliance
PubMed ID30456346
ß-Site APP-cleaving enzyme 1 (BACE1) inhibition is considered one of the most promising therapeutic strategies for Alzheimer's disease, but current BACE1 inhibitors also block BACE2. As the localization and function of BACE2 in the brain remain unknown, it is difficult to predict whether relevant side effects can be caused by ... More