Inhibition of caveolar uptake, SV40 infection, and beta1-integrin signaling by a nonnatural glycosphingolipid stereoisomer.
AuthorsSingh RD, Holicky EL, Cheng ZJ, Kim SY, Wheatley CL, Marks DL, Bittman R, Pagano RE
JournalJ Cell Biol
PubMed ID17371832
'Caveolar endocytosis is an important mechanism for the uptake of certain pathogens and toxins and also plays a role in the internalization of some plasma membrane (PM) lipids and proteins. However, the regulation of caveolar endocytosis is not well understood. We previously demonstrated that caveolar endocytosis and beta1-integrin signaling are ... More
Actin depolymerization transduces the strength of B-cell receptor stimulation.
AuthorsHao S, August A
JournalMol Biol Cell
PubMed ID15728723
'Polymerization of the actin cytoskeleton has been found to be essential for B-cell activation. We show here, however, that stimulation of BCR induces a rapid global actin depolymerization in a BCR signal strength-dependent manner, followed by polarized actin repolymerization. Depolymerization of actin enhances and blocking actin depolymerization inhibits BCR signaling, ... More
Silica crystals and aluminum salts activate the NALP3 inflammasome through phagosomal destabilization.
AuthorsHornung V, Bauernfeind F, Halle A, Samstad EO, Kono H, Rock KL, Fitzgerald KA, Latz E,
JournalNat Immunol
PubMed ID18604214
'Inhalation of silica crystals causes inflammation in the alveolar space. Prolonged exposure to silica can lead to the development of silicosis, an irreversible, fibrotic pulmonary disease. The mechanisms by which silica and other crystals activate immune cells are not well understood. Here we demonstrate that silica and aluminum salt crystals ... More
Bacterial RNA:DNA hybrids are activators of the NLRP3 inflammasome.
AuthorsKailasan Vanaja S, Rathinam VA, Atianand MK, Kalantari P, Skehan B, Fitzgerald KA, Leong JM,
Journal
PubMed ID24828532
'Enterohemorrhagic Escherichia coli (EHEC) is an extracellular pathogen that causes hemorrhagic colitis and hemolytic uremic syndrome. The proinflammatory cytokine, interleukin-1ß, has been linked to hemolytic uremic syndrome. Here we identify the nucleotide-binding domain and leucine rich repeat containing family, pyrin domain containing 3 (NLRP3) inflammasome as an essential mediator of ... More
Tracing from fat tissue, liver, and pancreas: a neuroanatomical framework for the role of the brain in type 2 diabetes.
AuthorsKreier F, Kap YS, Mettenleiter TC, van Heijningen C, van der Vliet J, Kalsbeek A, Sauerwein HP, Fliers E, Romijn JA, Buijs RM
JournalEndocrinology
PubMed ID16339209
'The hypothalamus uses hormones and the autonomic nervous system to balance energy fluxes in the body. Here we show that the autonomic nervous system has a distinct organization in different body compartments. The same neurons control intraabdominal organs (intraabdominal fat, liver, and pancreas), whereas sc adipose tissue located outside the ... More
High-resolution mapping of anatomical connections in marmoset extrastriate cortex reveals a complete representation of the visual field bordering dorsal V2.
AuthorsJeffs J, Federer F, Ichida JM, Angelucci A,
JournalCereb Cortex
PubMed ID22523183
The primate visual cortex consists of many areas. The posterior areas (V1, V2, V3, and middle temporal) are thought to be common to all primate species. However, the organization of cortex immediately anterior to area V2 (the ... More
Distinct roles of TIR and non-TIR regions in the subcellular localization and signaling properties of MyD88.
AuthorsNishiya T, Kajita E, Horinouchi T, Nishimoto A, Miwa S,
JournalFEBS Lett
PubMed ID17583698
MyD88 is a cytoplasmic adaptor protein that is critical for Toll-like receptor (TLR) signaling. The subcellular localization of MyD88 is characterized as large condensed forms in the cytoplasm. The mechanism and significance of this localization with respect to the signaling function, however, are currently unknown. Here, we demonstrate that MyD88 ... More
Glycoprotein H and a4ß1 Integrins Determine the Entry Pathway of Alphaherpesviruses.
AuthorsAzab W, Lehmann MJ, Osterrieder N,
JournalJ Virol
PubMed ID23514881
Herpesviruses enter cells either by direct fusion at the plasma membrane or from within endosomes, depending on the cell type and receptor(s). We investigated two closely related herpesviruses of horses, equine herpesvirus type 1 (EHV-1) and EHV-4, for which the cellular and viral determinants routing virus entry are unknown. We ... More
Membrane lipid composition and its physicochemical properties define cell vulnerability to aberrant protein oligomers.
AuthorsEvangelisti E, Cecchi C, Cascella R, Sgromo C, Becatti M, Dobson CM, Chiti F, Stefani M,
JournalJ Cell Sci
PubMed ID22344258
Increasing evidence suggests that the interaction of misfolded protein oligomers with cell membranes is a primary event resulting in the cytotoxicity associated with many protein-misfolding diseases, including neurodegenerative disorders. We describe here the results of a study on the relative contributions to toxicity of the physicochemical properties of protein oligomers ... More
NLRP3 inflammasomes are required for atherogenesis and activated by cholesterol crystals.
AuthorsDuewell P, Kono H, Rayner KJ, Sirois CM, Vladimer G, Bauernfeind FG, Abela GS, Franchi L, Nuñez G, Schnurr M, Espevik T, Lien E, Fitzgerald KA, Rock KL, Moore KJ, Wright SD, Hornung V, Latz E,
JournalNature
PubMed ID20428172
The inflammatory nature of atherosclerosis is well established but the agent(s) that incite inflammation in the artery wall remain largely unknown. Germ-free animals are susceptible to atherosclerosis, suggesting that endogenous substances initiate the inflammation. Mature atherosclerotic lesions contain macroscopic deposits of cholesterol crystals in the necrotic core, but their appearance ... More
A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale.
AuthorsBohland JW, Wu C, Barbas H, Bokil H, Bota M, Breiter HC, Cline HT, Doyle JC, Freed PJ, Greenspan RJ, Haber SN, Hawrylycz M, Herrera DG, Hilgetag CC, Huang ZJ, Jones A, Jones EG, Karten HJ, Kleinfeld D, Kötter R, Lester HA, Lin JM, Mensh BD, Mikula S, Panksepp J, Price JL, Safdieh J, Saper CB, Schiff ND, Schmahmann JD, Stillman BW, Svoboda K, Swanson LW, Toga AW, Van Essen DC, Watson JD, Mitra PP,
JournalPLoS Comput Biol
PubMed ID19325892
In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is critical, however, for both basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale ... More
The NALP3 inflammasome is involved in the innate immune response to amyloid-beta.
AuthorsHalle A, Hornung V, Petzold GC, Stewart CR, Monks BG, Reinheckel T, Fitzgerald KA, Latz E, Moore KJ, Golenbock DT,
JournalNat Immunol
PubMed ID18604209
The fibrillar peptide amyloid-beta (A beta) has a chief function in the pathogenesis of Alzheimer's disease. Interleukin 1 beta (IL-1 beta) is a key cytokine in the inflammatory response to A beta. Insoluble materials such as crystals activate the inflammasome formed by the cytoplasmic receptor NALP3, which results in the ... More
Novel anti-cholesterol monoclonal immunoglobulin G antibodies as probes and potential modulators of membrane raft-dependent immune functions.
AuthorsBíró A, Cervenak L, Balogh A, Lorincz A, Uray K, Horváth A, Romics L, Matkó J, Füst G, László G,
JournalJ Lipid Res
PubMed ID17023738
Natural autoantibodies against cholesterol are present in the sera of all healthy individuals; their function, production, and regulation, however, are still unclear. Here, we managed to produce two monoclonal anti-cholesterol antibodies (ACHAs) by immunizing mice with cholesterol-rich liposomes. The new ACHAs were specific to cholesterol and to some structurally closely ... More
Invariant computations in local cortical networks with balanced excitation and inhibition.
AuthorsMariño J, Schummers J, Lyon DC, Schwabe L, Beck O, Wiesing P, Obermayer K, Sur M
JournalNat Neurosci
PubMed ID15665876
Cortical computations critically involve local neuronal circuits. The computations are often invariant across a cortical area yet are carried out by networks that can vary widely within an area according to its functional architecture. Here we demonstrate a mechanism by which orientation selectivity is computed invariantly in cat primary visual ... More
Raft lipids as common components of human extracellular amyloid fibrils.
AuthorsGellermann GP, Appel TR, Tannert A, Radestock A, Hortschansky P, Schroeckh V, Leisner C, Lütkepohl T, Shtrasburg S, Röcken C, Pras M, Linke RP, Diekmann S, Fändrich M
JournalProc Natl Acad Sci U S A
PubMed ID15851687
Amyloid fibrils are fibrillar polypeptide aggregates from several degenerative human conditions, including Alzheimer's and Creutzfeldt-Jakob diseases. Analysis of amyloid fibrils derived from various human diseases (AA, ATTR, Abeta2M, ALlambda, and ALkappa amyloidosis) shows that these are associated with a common lipid component that has a conserved chemical composition and that ... More
Autophagy-mediated clearance of huntingtin aggregates triggered by the insulin-signaling pathway.
AuthorsYamamoto A, Cremona ML, Rothman JE
JournalJ Cell Biol
PubMed ID16505167
Conditional mouse models of polyglutamine diseases, such as Huntington's disease (HD), have revealed that cells can clear accumulated pathogenic proteins if the continuous production of the mutant transgene is halted. Invariably, the clearance of the protein leads to regression of the disease symptoms in mice. In light of these findings, ... More
Clathrin-independent internalization of the human histamine H1-receptor in CHO-K1 cells.
AuthorsSelf TJ, Oakley SM, Hill SJ
JournalBr J Pharmacol
PubMed ID16086035
The aim of the present study was to investigate the cellular pathway involved in histamine-stimulated internalization of the human H1-receptor in CHO-K1 cells expressing N-terminal myc-tagged H1-receptor (Myc-H1) or N-terminal myc-C-terminal green fluorescent protein (Myc-GFP H1) versions of the receptor. Studies of 3H-mepyramine binding and histamine-stimulated 3H-inositol phosphate accumulation in ... More
Proteomic and functional analyses of the periodic membrane skeleton in neurons.
Authors
JournalNat Commun
PubMed ID35680881
Bilateral visual projections exist in non-teleost bony fish and predate the emergence of tetrapods.
Authors
JournalScience
PubMed ID33833117
Spatially resolved cell atlas of the mouse primary motor cortex by MERFISH.
Authors
JournalNature
PubMed ID34616063
Single-cell analysis of pyroptosis dynamics reveals conserved GSDMD-mediated subcellular events that precede plasma membrane rupture.
Authors
JournalCell Death Differ
PubMed ID29666477
An amygdala-to-hypothalamus circuit for social reward.
Authors
JournalNat Neurosci
PubMed ID33820999
A multidimensional coding architecture of the vagal interoceptive system.
Authors
JournalNature
PubMed ID35296859
Serotonin engages an anxiety and fear-promoting circuit in the extended amygdala.
Authors
JournalNature
PubMed ID27556938
Posterior amygdala regulates sexual and aggressive behaviors in male mice.
Authors
JournalNat Neurosci
PubMed ID32719562
A fluorescence-based in vitro assay for investigating early endosome dynamics.
Authors
JournalNat Protoc
PubMed ID20539288
Dynamic caveolae exclude bulk membrane proteins and are required for sorting of excess glycosphingolipids.