'The presynaptic serotonin (5-HT) transporter (SERT) is targeted by widely prescribed antidepressant medications. Altered SERT expression or regulation has been implicated in multiple neuropsychiatric disorders, including anxiety, depression and autism. Here, we implement a generalizable strategy that exploits antagonist-conjugated quantum dots (Qdots) to monitor, for the first time, single SERT ... More
The formation of acetylcholine receptor clusters visualized with quantum dots.
AuthorsGeng L, Zhang HL, Peng HB,
JournalBMC Neurosci
PubMed ID19604411
BACKGROUND: Motor innervation of skeletal muscle leads to the assembly of acetylcholine receptor (AChR) clusters in the postsynaptic membrane at the vertebrate neuromuscular junction (NMJ). Synaptic AChR aggregation, according to the diffusion-mediated trapping hypothesis, involves the establishment of a postsynaptic scaffold that ... More
Co-localization of amyloid beta and tau pathology in Alzheimer's disease synaptosomes.
AuthorsFein JA, Sokolow S, Miller CA, Vinters HV, Yang F, Cole GM, Gylys KH,
JournalAm J Pathol
PubMed ID18467692
The amyloid cascade hypothesis proposes that amyloid beta (Abeta) pathology precedes and induces tau pathology, but the neuropathological connection between these two lesions has not been demonstrated. We examined the regional distribution and co-localization of Abeta and phosphorylated tau (p-tau) in synaptic terminals of Alzheimer's disease brains. To quantitatively examine ... More
A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale.
AuthorsBohland JW, Wu C, Barbas H, Bokil H, Bota M, Breiter HC, Cline HT, Doyle JC, Freed PJ, Greenspan RJ, Haber SN, Hawrylycz M, Herrera DG, Hilgetag CC, Huang ZJ, Jones A, Jones EG, Karten HJ, Kleinfeld D, Kötter R, Lester HA, Lin JM, Mensh BD, Mikula S, Panksepp J, Price JL, Safdieh J, Saper CB, Schiff ND, Schmahmann JD, Stillman BW, Svoboda K, Swanson LW, Toga AW, Van Essen DC, Watson JD, Mitra PP,
JournalPLoS Comput Biol
PubMed ID19325892
In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is critical, however, for both basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale ... More
Cholera toxin B subunit binding does not correlate with GM1 expression: a study using mouse embryonic neural precursor cells.
AuthorsYanagisawa M, Ariga T, Yu RK
JournalGlycobiology
PubMed ID16964630
Gangliosides, sialic acid-containing glycosphingolipids, are ubiquitously expressed in all eukaryotic cells and are primarily localized in the plasma membrane. Cholera toxin B subunit (Ctxb), a component of a heat-labile enterotoxin produced by Vibrio cholerae, has been frequently used as a probe to detect GM1 ganglioside because of its high affinity ... More
Tumor exosomes induce tunneling nanotubes in lipid raft-enriched regions of human mesothelioma cells.
Authors
JournalExp Cell Res
PubMed ID24468420
Role of endocytosis and trans-endocytosis in ICOS costimulator-induced downmodulation of the ICOS Ligand.
Authors
JournalJ Leukoc Biol
PubMed ID33527556
Profiling surface proteins on individual exosomes using a proximity barcoding assay.
Authors
JournalNat Commun
PubMed ID31451692
PLA2G1B is involved in CD4 anergy and CD4 lymphopenia in HIV-infected patients.
Authors
JournalJ Clin Invest
PubMed ID32436864
GM1 ganglioside-independent intoxication by Cholera toxin.
Authors
JournalPLoS Pathog
PubMed ID29432456
Endophilin-A2-dependent tubular endocytosis promotes plasma membrane repair and parasite invasion.
Dynamic heterogeneity and non-Gaussian statistics for ganglioside GM1s and acetylcholine receptors on live cell membrane.
Authors
JournalMol Biol Cell
PubMed ID32348189
Circulating extracellular vesicle-associated TGFβ3 modulates response to cytotoxic therapy in head and neck squamous cell carcinoma.
Authors
JournalCarcinogenesis
PubMed ID31436806
A preliminary investigation of circulating extracellular vesicles and biomarker discovery associated with treatment response in head and neck squamous cell carcinoma.