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Citations & References (58)
Invitrogen™
Cholera Toxin Subunit B (Recombinant), Horseradish Peroxidase Conjugate
Molecular Probes™ cholera toxin conjugates are made from a recombinant version of the B subunit only. This allows us toRead more
| Catalog Number | Quantity |
|---|---|
| C34780 also known as C-34780 | 100 μg |
Catalog number C34780
also known as C-34780
Price (CNY)
4,209.00
Each
Quantity:
100 μg
Price (CNY)
4,209.00
Each
Molecular Probes™ cholera toxin conjugates are made from a recombinant version of the B subunit only. This allows us to provide a very high-purity product that is completely free of the toxic A subunit. Cholera toxin B subunit (CT-B) attaches to cells by binding to ganglioside GM1, making it a powerful tool for retrograde labeling of neurons. This tracer has been used in a variety of applications, including tracing of rat forebrain afferents, projections of the parabrachial region, and neurons of the urinary bladder wall. When used in neuronal tracing applications, CT-B is typically introduced by pressure injection or by iontophoretic injection into neural tissue.
Cholera Toxin Subunit B Specifications:
• Label (Ex/Em): Horseradish peroxidase (HRP)
• At neutral pH, the 11.4 kDa B subunit exists as a 57 kDa pentamer
• Lyophilized product can be dissolved in buffer (e.g., PBS) for use
Cholera Toxin Subunit B for Studying Lipid Rafts
More recently, researchers have found that CT-B can be used as a marker for lipid rafts, which are membrane microdomains enriched in cholesterol and sphingolipids thought to be important in cell signaling. For lipid raft staining, cells are first incubated with fluorescent CT-B. Then, an anti–CT-B antibody is added to crosslink the CT-B in the lipid rafts into distinct patches on the plasma membrane. These patches are easily visualized by fluorescence microscopy. In addition to individual fluorescent CT-B conjugates, we also offer Vybrant™ Lipid Raft Labeling Kits that contain the Alexa Fluor™ 488, Alexa Fluor™ 555, or Alexa Fluor™ 594 dye conjugates of CT-B, an anti–CT-B antibody, and a detailed protocol for labeling and preparing cells for fluorescence microscopy.
Find More CT-B Conjugates
We offer various CT-B conjugates. Review Protein Conjugates—Section 14.7 in the Molecular Probes™ Handbook for more information on these tracers.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
Cholera Toxin Subunit B Specifications:
• Label (Ex/Em): Horseradish peroxidase (HRP)
• At neutral pH, the 11.4 kDa B subunit exists as a 57 kDa pentamer
• Lyophilized product can be dissolved in buffer (e.g., PBS) for use
Cholera Toxin Subunit B for Studying Lipid Rafts
More recently, researchers have found that CT-B can be used as a marker for lipid rafts, which are membrane microdomains enriched in cholesterol and sphingolipids thought to be important in cell signaling. For lipid raft staining, cells are first incubated with fluorescent CT-B. Then, an anti–CT-B antibody is added to crosslink the CT-B in the lipid rafts into distinct patches on the plasma membrane. These patches are easily visualized by fluorescence microscopy. In addition to individual fluorescent CT-B conjugates, we also offer Vybrant™ Lipid Raft Labeling Kits that contain the Alexa Fluor™ 488, Alexa Fluor™ 555, or Alexa Fluor™ 594 dye conjugates of CT-B, an anti–CT-B antibody, and a detailed protocol for labeling and preparing cells for fluorescence microscopy.
Find More CT-B Conjugates
We offer various CT-B conjugates. Review Protein Conjugates—Section 14.7 in the Molecular Probes™ Handbook for more information on these tracers.
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Label TypeOther Label(s) or Dye(s)
Protein FormRecombinant
Protein SubtypeCholera Toxin
Quantity100 μg
Shipping ConditionRoom Temperature
ConjugateHRP (Horseradish Peroxidase)
FormLyophilized
RecombinantRecombinant
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C).
Frequently asked questions (FAQs)
I injected a fluorescent tracer, but cannot detect it after tissue is fixed and sectioned. What am I doing wrong?
Do you have a tracer that will only transport retrograde?
How do I know which tracer to choose for my experiment?
What products do you have for neuronal tracing?
Citations & References (58)
Citations & References
Abstract
The mechanism of docosahexaenoic acid-induced phospholipase D activation in human lymphocytes involves exclusion of the enzyme from lipid rafts.
Journal:J Biol Chem
PubMed ID:12140281
'Docosahexaenoic acid (DHA), an n-3 polyunsaturated fatty acid that inhibits T lymphocyte activation, has been shown to stimulate phospholipase D (PLD) activity in stimulated human peripheral blood mononuclear cells (PBMC). To elucidate the mechanisms underlying the DHA-induced PLD activation, we first characterized the PLD expression pattern of PBMC. We show
Organization of motoneurons in the dorsal hypoglossal nucleus that innervate the retrusor muscles of the tongue in the rat.
Journal:Anat Rec
PubMed ID:9972807
'This anatomical investigation was prompted by the incomplete knowledge of the myotopic organization of the dorsal subdivison of the hypoglossal nucleus. Intrinsic muscle motoneurons were not segregated and labeled previously with regard to the lateral division of the hypoglossal nerve. Also, motoneuron number and cell size, in relation to the
Peripheral nerve lesion-induced uptake and transport of choleragenoid by capsaicin-sensitive c-fibre spinal ganglion neurons.
Journal:Acta Biol Hung
PubMed ID:12064782
'In the present experiments the effect of systemic capsaicin treatment on the retrograde labelling of sensory ganglion cells was studied following the injection of choleratoxin B subunit-horseradish peroxidase conjugate (CTX-HRP) into intact and chronically transected peripheral nerves. In the control rats CTX-HRP injected into intact sciatic nerves labelled medium and
Localization of amino acids, neuropeptides and cholinergic markers in neurons of the septum-diagonal band complex projecting to the retrosplenial granular cortex of the rat.
Journal:Brain Res Bull
PubMed ID:10974489
'Retrograde labelling was combined with immunohistochemistry to localize neurons containing choline acetyltransferase, gamma-aminobutyric acid (GABA), glutamate, leu-enkephalin, neurotensin, and substance P-like immunoreactivity in the projection pathways from the septum-diagonal band complex to the retrosplenial granular cortex in the rat. Injections of horseradish peroxidase conjugated to subunit B of cholera toxin
Synaptology and ultrastructural characteristics of laryngeal cricothyroid and posterior cricoarytenoid motoneurons in the nucleus ambiguus of the rat.
Journal:Anat Embryol (Berl)
PubMed ID:10463345
'The laryngeal motoneurons innervating the cricothyroid muscle (CT) are located in the semicompact formation just ventral to the rostral part of the compact formation of the nucleus ambiguus. The motoneurons innervating the posterior cricoarytenoid muscle (PCA) are located in the loose formation. We retrogradely labeled the CT and the PCA