The mechanism of docosahexaenoic acid-induced phospholipase D activation in human lymphocytes involves exclusion of the enzyme from lipid rafts.
AuthorsDiaz O, Berquand A, Dubois M, Di Agostino S, Sette C, Bourgoin S, Lagarde M, Nemoz G, Prigent AF
JournalJ Biol Chem
PubMed ID12140281
'Docosahexaenoic acid (DHA), an n-3 polyunsaturated fatty acid that inhibits T lymphocyte activation, has been shown to stimulate phospholipase D (PLD) activity in stimulated human peripheral blood mononuclear cells (PBMC). To elucidate the mechanisms underlying the DHA-induced PLD activation, we first characterized the PLD expression pattern of PBMC. We show ... More
Organization of motoneurons in the dorsal hypoglossal nucleus that innervate the retrusor muscles of the tongue in the rat.
AuthorsMcClung JR, Goldberg SJ
JournalAnat Rec
PubMed ID9972807
'This anatomical investigation was prompted by the incomplete knowledge of the myotopic organization of the dorsal subdivison of the hypoglossal nucleus. Intrinsic muscle motoneurons were not segregated and labeled previously with regard to the lateral division of the hypoglossal nerve. Also, motoneuron number and cell size, in relation to the ... More
Peripheral nerve lesion-induced uptake and transport of choleragenoid by capsaicin-sensitive c-fibre spinal ganglion neurons.
AuthorsJancsó G, Sántha P, Gecse K
JournalActa Biol Hung
PubMed ID12064782
'In the present experiments the effect of systemic capsaicin treatment on the retrograde labelling of sensory ganglion cells was studied following the injection of choleratoxin B subunit-horseradish peroxidase conjugate (CTX-HRP) into intact and chronically transected peripheral nerves. In the control rats CTX-HRP injected into intact sciatic nerves labelled medium and ... More
Localization of amino acids, neuropeptides and cholinergic markers in neurons of the septum-diagonal band complex projecting to the retrosplenial granular cortex of the rat.
AuthorsGonzalo-Ruiz A, Morte L
JournalBrain Res Bull
PubMed ID10974489
'Retrograde labelling was combined with immunohistochemistry to localize neurons containing choline acetyltransferase, gamma-aminobutyric acid (GABA), glutamate, leu-enkephalin, neurotensin, and substance P-like immunoreactivity in the projection pathways from the septum-diagonal band complex to the retrosplenial granular cortex in the rat. Injections of horseradish peroxidase conjugated to subunit B of cholera toxin ... More
Synaptology and ultrastructural characteristics of laryngeal cricothyroid and posterior cricoarytenoid motoneurons in the nucleus ambiguus of the rat.
AuthorsHayakawa T, Zheng JQ, Maeda S, Ito H, Seki M, Yajima Y
JournalAnat Embryol (Berl)
PubMed ID10463345
'The laryngeal motoneurons innervating the cricothyroid muscle (CT) are located in the semicompact formation just ventral to the rostral part of the compact formation of the nucleus ambiguus. The motoneurons innervating the posterior cricoarytenoid muscle (PCA) are located in the loose formation. We retrogradely labeled the CT and the PCA ... More
Systemic administration of cholera toxin B subunit conjugated to horseradish peroxidase in the adult rat labels preganglionic autonomic neurons, motoneurons, and select primary afferents for light and electron microscopic studies.
AuthorsHavton LA, Broman J,
JournalJ Neurosci Methods
PubMed ID16054225
'Retrograde and transganglionic labeling techniques are commonly used to visualize subsets of neurons and sensory afferent projections in the nervous system. These methods commonly require anesthesia and surgical methods. However, some tracers can also be administered systemically in awake animals, thus reducing risks associated with anesthesia and surgery and allowing ... More
Long-term neuroprotective effects of glycosaminoglycans-IGF-I cotreatment in the motor neuron degeneration (mnd) mutant mouse.
AuthorsGorio A, Germani E, Lesma E, Rossoni G, Muller EE, Di Giulio AM
JournalEur J Neurosci
PubMed ID10564347
'This study shows that cotreatment with insulin-like growth factor-I (IGF-I) and glycosaminoglycans (GAGs) prevents the onset of neuromuscular deficit in the m/m mutant mouse. These mice show a mid-to-late-life onset of progressive paralysis of the hind limb, that is correlated with altered innervation and reduced nerve-evoked isometric twitch tension in ... More
Organization and neurochemistry of vagal preganglionic neurons innervating the lower esophageal sphincter in ferrets.
'The motor control of the lower esophageal sphincter (LES) is critical for normal swallowing and emesis, as well as for the prevention of gastroesophageal reflux. However, there are surprisingly few data on the central organization and neurochemistry of LES-projecting preganglionic neurons. There are no such data in ferrets, which are ... More
Monosynaptic inputs from the nucleus tractus solitarii to the laryngeal motoneurons in the nucleus ambiguus of the rat.
AuthorsHayakawa T, Takanaga A, Maeda S, Ito H, Seki M
JournalAnat Embryol (Berl)
PubMed ID11089932
'The cricothyroid (CT) and the posterior cricoarytenoid (PCA) muscles in the larynx are activated by the laryngeal motoneurons located within the nucleus ambiguus; these motoneurons receive the laryngeal sensory information from the nucleus tractus solitarii (NTS) during respiration and swallowing. We investigated whether the neurons in the NTS projected directly ... More
Ultrastructure of the central subnucleus of the nucleus tractus solitarii and the esophageal afferent terminals in the rat.
AuthorsHayakawa T, Takanaga A, Tanaka K, Maeda S, Seki M
JournalAnat Embryol (Berl)
PubMed ID12649725
'The central subnucleus of the nucleus tractus solitarii (ceNTS) receives afferent projections from the esophageal wall and projects to the nucleus ambiguus, thus serving as a relay nucleus for peristalsis of the esophagus. Here we examine the synaptic organization of the ceNTS, and its esophageal afferents by using transganglionic anterograde ... More
Astaxanthine secured apoptotic death of PC12 cells induced by beta-amyloid peptide 25-35: its molecular action targets.
'Astaxanthine (ASTx) is a novel carotenoid nutraceutical occurring in many crustaceans and red yeasts. It has potent antioxidant, photoprotective, hepatodetoxicant, and anti-inflammatory activities. Documented effect of ASTx on treatment of neurodegenerative disease is still lacking. We used the beta-amyloid peptide (Abeta) 25-35-treated PC12 model to investigate the neuron-protective effect of ... More
Androgen stimulates neuronal plasticity in the perineal motoneurons of aged male rats.
AuthorsMatsumoto A
JournalJ Comp Neurol
PubMed ID11169475
'Ten aged male rats (24 months of age) were castrated and implanted subcutaneously with Silastic capsules containing testosterone (T)(5 males) or nothing (5 males). Five sham-castrated males (25 months of age) served as controls. Four weeks after castration, cholera toxin-horseradish peroxidase (CT-HRP) was injected into the bulbocavernosus muscles and animals ... More
N-methyl-D-aspartate receptor blockade inhibits estrogenic support of dendritic growth in a sexually dimorphic rat spinal nucleus.
AuthorsHebbeler SL, Verhovshek T, Sengelaub DR
JournalJ Comp Neurol
PubMed ID12209833
'The lumbar spinal cord of rats contains the sexually dimorphic, steroid-sensitive spinal nucleus of the bulbocavernosus (SNB). Dendritic development of SNB motoneurons requires the action of both androgens and estrogens. Estrogenic effects are limited to the initial growth of SNB dendrites through 4 weeks of age. During this postnatal period, ... More
Long-term regeneration of abdominal vagus: efferents fail while afferents succeed.
AuthorsPhillips RJ, Baronowsky EA, Powley TL
JournalJ Comp Neurol
PubMed ID12454987
'Vagal afferents regenerate, by 18 weeks after subdiaphragmatic transection, to reinnervate the gut and to differentiate into the two types of terminals normally found in the smooth muscle wall of the gastrointestinal (GI) tract (Phillips et al. [2000] J Comp Neurol. 421:325-346). Regeneration, however, is neither complete nor entirely accurate ... More
Evidence that androgen acts through NMDA receptors to affect motoneurons in the rat spinal nucleus of the bulbocavernosus.
AuthorsJordan CL, Christensen SE, Handa RJ, Anderson JL, Pouliot WA, Breedlove SM
JournalJ Neurosci
PubMed ID12417681
'In adult male rats, spinal nucleus of the bulbocavernosus (SNB) motoneurons shrink after castration and are restored in size after androgen treatment. Sixty-day-old Sprague Dawley males were castrated and implanted with SILASTIC capsules containing testosterone (T) or nothing, and osmotic minipumps continuously infusing MK-801, a noncompetitive NMDA receptor antagonist, or ... More
Retrograde and transganglionic transport of horseradish peroxidase-conjugated cholera toxin B subunit, wheatgerm agglutinin and isolectin B4 from Griffonia simplicifolia I in primary afferent neurons innervating the rat urinary bladder.
AuthorsWang HF, Shortland P, Park MJ, Grant G
JournalNeuroscience
PubMed ID9722157
'In the present study, we investigated and compared the ability of the cholera toxin B subunit, wheat germ agglutinin and isolectin B4 from Griffonia simplicifolia I conjugated to horseradish peroxidase, to retrogradely and transganglionically label visceral primary afferents after unilateral injections into the rat urinary bladder wall. Horseradish peroxidase histochemical ... More
Resection of sciatic nerve re-triggers central sprouting of A-fibre primary afferents in the rat.
AuthorsMa QP, Tian L, Woolf CJ
JournalNeurosci Lett
PubMed ID10889346
'We have investigated whether nerve injury or target deprivation is responsible for the injury induced central sprouting of A-fibres. Cholera toxin B subunit conjugated horseradish peroxidase was used to trace the termination of A-fibre primary afferents. Transection of the sciatic nerve induces central sprouting of sciatic myelinated A-fibre primary afferents ... More
A-fibres sprouting from lamina I into lamina II of spinal dorsal horn after peripheral nerve injury in rats.
AuthorsMa QP, Tian L
JournalBrain Res
PubMed ID11516419
We have examined the labeling pattern in the spinal dorsal horn by an intra-sciatic nerve injection of cholera toxin B subunit conjugated horseradish peroxidase (HRP) after transection of the posterior cutaneous nerve and inferior gluteal nerve, and found that the cholera toxin B subunit conjugated HRP labeling in lamina I ... More
Cholera toxin B subunit labeling in lamina II of spinal cord dorsal horn following chronic inflammation in rats.
AuthorsMa QP, Tian L
JournalNeurosci Lett
PubMed ID12113902
We have investigated the effect of inflammation on the labeling pattern of cholera toxin B subunit (CTB)-conjugated horseradish peroxidase, an A-fiber marker, by an intra-sciatic nerve injection of the tracer. Following chronic inflammation in one hind paw in rats, there was substantial CTB labeling in lamina II of the spinal ... More
The methodology for labeling the distal cerebrospinal fluid-contacting neurons in rats.
AuthorsLu X, Geng X, Zhang L, Zeng Y,
JournalJ Neurosci Methods
PubMed ID18179825
The distal cerebrospinal fluid-contacting neurons (dCSF-CNs) are one special type of neurons, whose bodies are in the parenchyma of the brain and processes extend into the ventricle, which suggested that dCSF-CNs might play the important roles in neuromodulation and neuroendocrinal regulation. Without special tracing method, the dCSF-CNs is hard to ... More
A proposal for a coordinated effort for the determination of brainwide neuroanatomical connectivity in model organisms at a mesoscopic scale.
AuthorsBohland JW, Wu C, Barbas H, Bokil H, Bota M, Breiter HC, Cline HT, Doyle JC, Freed PJ, Greenspan RJ, Haber SN, Hawrylycz M, Herrera DG, Hilgetag CC, Huang ZJ, Jones A, Jones EG, Karten HJ, Kleinfeld D, Kötter R, Lester HA, Lin JM, Mensh BD, Mikula S, Panksepp J, Price JL, Safdieh J, Saper CB, Schiff ND, Schmahmann JD, Stillman BW, Svoboda K, Swanson LW, Toga AW, Van Essen DC, Watson JD, Mitra PP,
JournalPLoS Comput Biol
PubMed ID19325892
In this era of complete genomes, our knowledge of neuroanatomical circuitry remains surprisingly sparse. Such knowledge is critical, however, for both basic and clinical research into brain function. Here we advocate for a concerted effort to fill this gap, through systematic, experimental mapping of neural circuits at a mesoscopic scale ... More
Horseradish peroxidase: a modern view of a classic enzyme.
AuthorsVeitch NC,
JournalPhytochemistry
PubMed ID14751298
Horseradish peroxidase is an important heme-containing enzyme that has been studied for more than a century. In recent years new information has become available on the three-dimensional structure of the enzyme and its catalytic intermediates, mechanisms of catalysis and the function of specific amino acid residues. Site-directed mutagenesis and directed ... More
Ultrastructural analysis of spinal primary afferent fibers within the circular muscle of the cat lower esophageal sphincter.
AuthorsMazzia C, Clerc N
JournalHistochem Cell Biol
PubMed ID10817678
We have analyzed the ultrastructural characteristics and environment of spinal primary afferent fibers that run within the circular muscle of the cat lower esophageal sphincter. These were selectively labeled by anterogradely transported cholera toxin B subunit conjugated with horseradish peroxidase. Most of the labeled fibers were perpendicular to the muscle ... More
Localization of a serine proteinase inhibitor, B-43, in the bovine pancreas.
AuthorsNishibori M, Nakaya N, Ohtsuka A, Murakami T, Saeki K
JournalHistochem Cell Biol
PubMed ID9681689
B-43, a serine proteinase inhibitor belonging to the ovalbumin branch of the serpin superfamily, was purified and cloned from bovine brain. Since [35S]-labeled B-43 forms SDS-stable complexes with pancreatic serine proteinases, trypsin, alpha-chymotrypsin, and kallikrein, it has been suggested that B-43 is capable of inhibiting these serine proteinases and that ... More
Synaptic changes in the perineal motoneurons of aged male rats.
AuthorsMatsumoto A
JournalJ Comp Neurol
PubMed ID9762869
Cholera toxin-horseradish peroxidase (CT-HRP) was injected into the bulbocavernosus muscles of young (2 months of age) and old (19-20 months of age) male rats, and animals were killed 2 days later. The spinal cords containing the spinal nucleus of the bulbocavernosus (SNB) were dissected, processed with a modified tetramethylbenzidine method ... More
Neuronal sensitivity to tetanus toxin requires gangliosides.
AuthorsWilliamson LC, Bateman KE, Clifford JC, Neale EA
JournalJ Biol Chem
PubMed ID10455200
Tetanus toxin produces spastic paralysis in situ by blocking inhibitory neurotransmitter release in the spinal cord. Although di- and trisialogangliosides bind tetanus toxin, their role as productive toxin receptors remains unclear. We examined toxin binding and action in spinal cord cell cultures grown in the presence of fumonisin B(1), an ... More
Use of magnetic beads in selection and detection of biotoxin aptamers by electrochemiluminescence and enzymatic methods.
AuthorsBruno JG, Kiel JL
JournalBiotechniques
PubMed ID11808691
Systematic evolution of ligands by exponential enrichment (SELEX) was used to develop DNA ligands (aptamers) to cholera whole toxin and staphylococcal enterotoxin B (SEB). Affinity selection of aptamers was accomplished by conjugating the biotoxins to tosyl-activated magnetic beads. The use of magnetic beads reduces the volumes needed to perform aptamer ... More
Effects of intranasal administration of cholera toxin (or Escherichia coli heat-labile enterotoxin) B subunits supplemented with a trace amount of the holotoxin on the brain.
AuthorsHagiwara Y, Iwasaki T, Asanuma H, Sato Y, Sata T, Aizawa C, Kurata T, Tamura S
JournalVaccine
PubMed ID11166888
Effects of intranasal administration of cholera toxin (CT) [or Escherichia coli heat-labile enterotoxin (LT)] B subunits supplemented with a trace amount of the holotoxin, CTB* or LTB*, on the brain were examined in BALB/c mice by comparing with those of the intracerebral injection. Intracerebral injection of CTB* at doses more ... More
Aromatase inhibition reduces dendritic growth in a sexually dimorphic rat spinal nucleus.
AuthorsBurke KA, Kuwajima M, Sengelaub DR
JournalJ Neurobiol
PubMed ID10022574
The rat lumbar spinal cord contains the steroid-sensitive spinal nucleus of the bulbocavernosus (SNB), whose motoneurons innervate perineal muscles involved in copulatory reflexes. In normal males, SNB motoneuron dendrites grow exuberantly through postnatal (P) day 28. This growth is steroid dependent: Dendrites fail to grow in males castrated at P7, ... More
Does the perireticular thalamic nucleus project to the neocortex?
AuthorsColeman KA, Mitrofanis J
JournalAnat Embryol (Berl)
PubMed ID10526020
This study defines several features of the early connections of the developmentally transient perireticular thalamic nucleus of rats. The neocortex of developing rats was injected with either DiI, biotinylated dextran, WGA-HRP (wheatgerm agglutinin conjugated-horseradish peroxidase), fluorescent latex beads or cholera toxin subunit B (CTB) and their brains were processed for ... More
Terminals of paraventricular spinal neurones are closely associated with adrenal medullary sympathetic preganglionic neurones: immunocytochemical evidence for vasopressin as a possible neurotransmitter in this pathway.
AuthorsMotawei K, Pyner S, Ranson RN, Kamel M, Coote JH
JournalExp Brain Res
PubMed ID10333008
A recent study using transsynaptically transported pseudorabies virus, injected into the adrenal gland, showed labelled neurones in the paraventricular nucleus (PVN) of the hypothalamus, indicating that these neurones send projections to sympathoadrenal preganglionic neurones (SPNs). However, this technique cannot conclusively demonstrate that the pathway is monosynaptic. In order to investigate ... More
Myelinated afferents sprout into lamina II of L3-5 dorsal horn following chronic constriction nerve injury in rats.
AuthorsNakamura S, Myers RR
JournalBrain Res
PubMed ID10082814
In order to investigate the consequences of chronic constriction injury (CCI) to nerve, we explored the relationship between the development of mechanical allodynia and the reorganization of primary afferent terminals in the sensory lamina of the rat spinal cord dorsal horn. Following sciatic CCI neuropathy, mechanical allodynia developed in the ... More
Pre-embedding staining for GAD67 versus postembedding staining for GABA as markers for central GABAergic terminals.
AuthorsMurphy SM, Pilowsky PM, Llewellyn-Smith IJ
JournalJ Histochem Cytochem
PubMed ID9774625
Pre-embedding immunocytochemistry for the active form of glutamate decarboxylase (GAD67) and postembedding staining for gamma-aminobutyric acid (GABA) were compared as markers for central GABAergic terminals in the phrenic motor nucleus, in which phrenic motor neurons had been retrogradely labeled with cholera toxin B-horseradish peroxidase. Nerve terminals with or without GAD67 ... More
On the problem of lamination in the superficial dorsal horn of mammals: a reappraisal of the substantia gelatinosa in postnatal life.
AuthorsWoodbury CJ, Ritter AM, Koerber HR
JournalJ Comp Neurol
PubMed ID10660890
Although it is one of the most distinctive and earliest recognized features in the spinal cord, the substantia gelatinosa (SG) remains among the most enigmatic of central nervous system regions. The present neuroanatomical studies employed transganglionic transport of horseradish peroxidase conjugates of choleragenoid (B-HRP) and the B4 isolectin of Bandeiraea ... More
Perineal muscles and motoneurons are sexually monomorphic in the naked mole-rat (Heterocephalus glaber).
AuthorsPeroulakis ME, Goldman B, Forger NG
JournalJ Neurobiol
PubMed ID11920726
Naked mole-rats are eusocial mammals that live in colonies with a single breeding female and one to three breeding males. All other members of the colony, known as subordinates, are nonreproductive and exhibit few sex differences in behavior or genital anatomy. This raises questions about the degree of sexual differentiation ... More
A fast, simple and sensitive method for the detection and quantification of detergent-resistant membranes.
AuthorsBlank N, Gabler C, Schiller M, Kriegel M, Kalden JR, Lorenz HM
JournalJ Immunol Methods
PubMed ID12445726
The aggregation of the T cell receptor and other signaling molecules leads to the formation of large molecular activation clusters in the cell membrane. These molecular clusters are associated with a high concentration of cholesterol, sphingomyelin and gangliosides and were referred to as lipid microdomains. Electron microscopy studies of viable ... More
Substance P afferent terminals innervate vagal preganglionic neurons projecting to the trachea of the ferret.
AuthorsMassari VJ, Haxhiu MA
JournalAuton Neurosci
PubMed ID11958475
Airway disorders, such as asthma and chronic obstructive bronchitis, are, in part, due to abnormalities in the nervous control of the airways. However, the ultrastructural circuitry and neurochemical anatomy of afferents modulating the output of airway-related vagal preganglionic neurons (VPNs) in the nucleus ambiguus are poorly understood. We have examined ... More
Immunoelectron microscopic study of gamma-aminobutyric acid inputs to identified thalamocortical projection neurons in the anterior thalamus of the rat.
AuthorsWang B, Gonzalo-Ruiz A, Sanz JM, Campbell G, Lieberman AR
JournalExp Brain Res
PubMed ID10382622
We have carried out a semi-quantitative ultrastructural study to determine the characteristics and distribution of gamma-aminobutyric acid (GABA)-containing constituents of the anterodorsal (AD) and anteroventral (AV) thalamic nuclei in adult rats. We used a polyclonal antibody to GABA and a postembedding immunogold detection method in animals in which the cortical ... More
Anatomical and functional study of localization of originating neurons of the parasympathetic nerve to gallbladder in rabbit brain stem.
AuthorsLi AJ, Liu JZ, Liu CY
JournalChin J Physiol
PubMed ID12005348
The present study was to investigate the localization of preganglionic parasympathetic neurons of gallbladder in brain stem by anatomical and functional approaches. Male or female rabbits (n = 11) were anesthetized with sodium pentobarbital (30 mg/kg, i.v.). Cholera toxin B conjugated to horseradish peroxidase (CB-HRP) was injected into the gallbladder ... More
A-fiber sprouting in spinal cord dorsal horn is attenuated by proximal nerve stump encapsulation.
AuthorsWhite FA, Kocsis JD
JournalExp Neurol
PubMed ID12429185
Peripheral nerve transection in the rat alters the spinal cord dorsal horn central projections from both small and large DRG neurons. Injured neurons with C-fibers exhibit transganglionic degeneration of their terminations within lamina II of the spinal cord dorsal horn, while peripheral nerve injury of medium to large neurons induces ... More
Brainstem viscerotopic organization of afferents and efferents involved in the control of swallowing.
AuthorsBroussard DL, Altschuler SM
JournalAm J Med
PubMed ID10718457
Cholera toxin horseradish peroxidase (CT-HRP), a sensitive antegrade and retrograde tracer, is effective at labeling swallowing motoneurons and their dendritic fields within the nucleus ambiguus (NA), nucleus of the solitary tract (NTS), dorsal motor nucleus of the vagus nerve, and hypoglossal nucleus. Using this tracer to label motoneurons within the ... More
Do synaptic rearrangements underlie compensatory reflex enhancement in spinal motoneurons after partial cell loss?
AuthorsHolmberg P, Kellerth JO
JournalSynapse
PubMed ID11044885
In adult cats, avulsion of a spinal ventral root induces retrograde cell death among the corresponding motoneurons and, also, enhanced monosynaptic reflexes ipsilaterally in the adjacent uninjured spinal cord segments. The present study investigates possible mechanisms behind this reflex potentiation. At 1-12 weeks after unilateral L7 ventral root avulsion, the ... More
Motoneurons of the lateral and medial rectus extraocular muscles in squirrel monkey and cat.
AuthorsMcClung JR, Shall MS, Goldberg SJ
JournalCells Tissues Organs
PubMed ID11173806
The goal of this study was to examine and compare the number and size of motoneurons in the cat and squirrel monkey abducens nucleus. We also examined medial rectus muscle motoneuron compartmentalization in the squirrel monkey oculomotor nucleus and compared those cells to abducens nucleus motoneurons. Retrograde labeling of the ... More
Castration reduces motoneuron soma size but not dendritic length in the spinal nucleus of the bulbocavernosus of wild-type and BCL-2 overexpressing mice.
AuthorsPark JJ, Zup SL, Verhovshek T, Sengelaub DR, Forger NG
JournalJ Neurobiol
PubMed ID12382267
Motoneurons in the spinal nucleus of the bulbocavernosus (SNB) and their target muscles, bulbocavernosus and levator ani (BC/LA), constitute an androgen-sensitive neuromuscular system. Testosterone regulates SNB soma size, SNB dendritic length, and BC/LA muscle mass in adult male rats. Recent evidence indicates that the cell death-regulatory protein, Bcl-2, may also ... More
Laryngeal and respiratory protective reflexes.
AuthorsAltschuler SM
JournalAm J Med
PubMed ID11749932
Swallowing is a complex motor behavior that relies on an interneuronal network of premotor neurons (PMNs) to organize the sequential activity of motor neurons that are active during the buccopharyngeal and esophageal phases. Swallowing PMNs are highly interconnected to multiple areas of the brain stem and the central nervous system ... More
Glutamatergic components of the retrosplenial granular cortex in the rat.
AuthorsWang B, Gonzalo-Ruiz A, Sanz JM, Campbell G, Lieberman AR
JournalJ Neurocytol
PubMed ID11951053
The ultrastructural characteristics, distribution and synaptic relationships of identified, glutamate-enriched thalamocortical axon terminals and cell bodies in the retrosplenial granular cortex of adult rats is described and compared with GABA-containing terminals and cell bodies, using postembedding immunogold immunohistochemistry and transmission electron microscopy in animals with injections of cholera toxin- horseradish ... More
As the gut ages: timetables for aging of innervation vary by organ in the Fischer 344 rat.
AuthorsPhillips RJ, Powley TL
JournalJ Comp Neurol
PubMed ID11331534
To explore the effects of aging on the vagal innervation of the gastrointestinal (GI) tract, male Fischer 344 rats at 3 and 24 months of age were injected in the left nodose ganglion with 3 microl of either 4% wheat germ agglutinin-horseradish peroxidase (to label sensory endings) or 1% cholera ... More
The origin of catecholaminergic nerve fibers in the subdiaphragmatic vagus nerve of rat.
AuthorsYang M, Zhao X, Miselis RR
JournalJ Auton Nerv Syst
PubMed ID10412834
It is known that the vagus nerve contains catecholaminergic fibers. However, the origin of these fibers has not been systematically examined. In this study, we addressed this issue using retrograde tracing from the subdiaphragmatic vagus nerve combined with immunocytochemistry. The cervical and thoracic sympathetic trunk ganglia, the nodose ganglia and ... More
Identification of signal substances in synapses made between primary afferents and their associated axon terminals in the rat trigeminal sensory nuclei.
AuthorsBae YC, Ihn HJ, Park MJ, Ottersen OP, Moritani M, Yoshida A, Shigenaga Y
JournalJ Comp Neurol
PubMed ID10701828
The relationships between primary afferent terminals (PATs) and their associated presynaptic terminals in the rat trigeminal sensory nuclear complex (TSNC) were examined with special reference to amino acid transmitters glutamate (Glu) and gamma-aminobutyric acid (GABA). Primary afferent terminals anterogradely labeled from the trigeminal ganglion with the B subunit of cholera ... More
Immunoelectron microscopic study of glutamate inputs from the retrosplenial granular cortex to identified thalamocortical projection neurons in the anterior thalamus of the rat.
AuthorsWang B, Gonzalo-Ruiz A, Morte L, Campbell G, Lieberman AR
JournalBrain Res Bull
PubMed ID10507474
We have carried out an ultrastructural study to determine the characteristics and distribution of glutamate-containing constituents of the anterodorsal (AD) and anteroventral (AV) thalamic nuclei in adult rats. We used a polyclonal antibody to glutamate and a postembedding immunogold detection method in animals in which the neurons of AD/AV projecting ... More
Lipid raft localization of epidermal growth factor receptor alters matrix metalloproteinase-1 expression in SiHa cells via the MAPK/ERK signaling pathway.
Authors
JournalOncol Lett
PubMed ID28101233
Sterol carrier protein 2 regulates proximal tubule size in the Xenopus pronephric kidney by modulating lipid rafts.
Authors
JournalDev Biol
PubMed ID25127994
p75(NTR) mediates ephrin-A reverse signaling required for axon repulsion and mapping.
Authors
JournalNeuron
PubMed ID18786358
Localization and proteomic characterization of cholesterol-rich membrane microdomains in the inner ear.
Authors
JournalJ Proteomics
PubMed ID24713161
Impaired Levels of Gangliosides in the Corpus Callosum of Huntington Disease Animal Models.
Authors
JournalFront Neurosci
PubMed ID27766070
Corticocuneate projections are altered after spinal cord dorsal column lesions in New World monkeys.
Authors
JournalJ Comp Neurol
PubMed ID33029803
Cooperative function of synaptophysin and synapsin in the generation of synaptic vesicle-like clusters in non-neuronal cells.
Authors
JournalNat Commun
PubMed ID33431828
Altered Expression of Ganglioside Metabolizing Enzymes Results in GM3 Ganglioside Accumulation in Cerebellar Cells of a Mouse Model of Juvenile Neuronal Ceroid Lipofuscinosis.