CyQUANT™ NF 细胞增殖检测
CyQUANT™ NF 细胞增殖检测
Invitrogen™

CyQUANT™ NF 细胞增殖检测

CyQUANT™ NF 细胞增殖检测提供了在微孔板内对群体内细胞进行定量的快速灵敏方法。CyQUANT™ NF 细胞增殖检测的特点为：•比 MTT 或 AlamarBlue™ 测定试剂盒更灵敏•线性检测范围为每孔100至20,000个细胞了解更多信息
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货号细胞类型数量
C35011Direct Cell10 Microplates
C35013Direct Red Cell10块微孔板
C35012Direct Cell100 Microplates
C35007NF Cell200 Assays
C7026For cells in culture1000 次检测
C35006NF Cell1000 Assays
C7027Cell Lysis Buffer50 mL
货号 C35011
价格（CNY)
7,538.00
Each
添加至购物车
细胞类型:
Direct Cell
数量:
10 Microplates
价格（CNY)
7,538.00
Each
添加至购物车
CyQUANT™ NF 细胞增殖检测提供了在微孔板内对群体内细胞进行定量的快速灵敏方法。

CyQUANT™ NF 细胞增殖检测的特点为：

•比 MTT 或 AlamarBlue™ 测定试剂盒更灵敏
•线性检测范围为每孔100至20,000个细胞（96孔微孔板）
•测定试剂盒可以在一小时内完成

快速简单的细胞增殖检测方法
CyQUANT™ NF 细胞增殖测定试剂盒不需要细胞裂解、长时间孵育、放射性标记或从细胞中去除染色剂。CyQUANT™ NF 检测无需进行原始 CyQUANT™ 细胞增殖检测的冻融细胞裂解步骤，代之以一种细胞通透性的 DNA 结合染料和一种质膜通透性的试剂。CyQUANT™ NF 细胞增殖测定试剂盒可以在96孔或384孔微孔板内使用，有两种包装形式：200次测定试剂盒 (C35007)，适用于较小样本量，以及1000次测定试剂盒 (C35006)，适用于高通量应用。

AlamarBlue™ 是 TREK Diagnostic Systems 公司的注册商标。
仅供科研使用。不可用于诊断程序。
规格
细胞类型Direct Cell
培养环境悬浮细胞培养、贴壁细胞培养
检测方法荧光
染料类型其他标记或染料
激发/发射508/527 nm
产品规格96 孔板、1536 孔板、384 孔板
孵育时间60 min
数量10 Microplates
运输条件室温
颜色绿色
发射可见光
适用于（应用）增殖测定试剂盒
适用于（设备）微孔板读数仪, 分光光度计, 显微镜, HTS 读数仪
产品线CyQUANT™、Molecular Probes™
产品类型细胞增殖测定试剂盒
Unit SizeEach
内容与储存
  • 0.5 mL CyQUANT™ Direct 核酸染色剂（组分 A）
  • 2.5 ml CyQUANT™ Direct 背景抑制剂 I（组分 B）
  • 2°C 到25°C 下储存。干燥避光保存。

常见问题解答 (FAQ)

你们提供测定神经元细胞健康的产品吗？

可采用PrestoBlue细胞活力染色剂和CyQUANT细胞增殖检测试剂盒。我们还提供神经突生长染色试剂盒（货号A15001）。更多有关我们的神经元细胞健康检测试剂的信息请见此处（https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html）。

其他的细胞活性检测试剂能与AlgiMatrix 基质一起使用吗？

除了Invitrogen alamarBlue 细胞活力检测试剂（Cell Viability Reagent）之外，AlgiMatrix 基质还能够与Invitrogen PrestoBlue 细胞活力检测试剂，Promega  Cell Titer-Glo 试剂或Invitrogen CyQuant  Direct Cell Proliferation Assay试剂盒相兼容。具体实验方案与2D细胞培养系统几乎相同，但需要加入纯AlgiMatrix 基质作为空白对照组。

MTT cell proliferation plate assays require cellular metabolism to modify the reagent and thus, only live cells will be counted. Is this true for CyQUANT Direct Cell Proliferation Assay, too?

This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye in it is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a non-cell-permeable quenching reagent in the kit which will both quench extracellular fluorescence (and thus this is a no-wash assay) AND will quench the fluorescence in dead cells (but not live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

MTT cell proliferation assays require cellular metabolism to turn over the reagent, and thus only live cells will be counted. Is this true for CyQUANT Direct as well?

This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a cell impermeant quenching reagent in the kit which will quench both extracellular fluorescence (and thus this is a no-wash assay) and the fluorescence in dead cells (but not live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What volumes should I use to make a 10X stock solution for the CyQUANT Direct Cell Proliferation Assay (Cat. No. C35011, C35012)?

To make a 10X stock solution for the CyQUANT Direct Cell Proliferation Assay (Cat. No. C35011, C35012), we suggest using the following volumes:

1.1 mL         PBS
25 µL         CyQUANT Direct nucleic acid stain (Component A)
125 µL         CyQUANT Direct background suppressor I (Component B)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all CyQUANT™ NF 细胞增殖检测 FAQs