CyQUANT™ Direct Cell Proliferation Assay, 10 microplates kit - FAQs

可采用PrestoBlue细胞活力染色剂和CyQUANT细胞增殖检测试剂盒。我们还提供神经突生长染色试剂盒（货号A15001）。更多有关我们的神经元细胞健康检测试剂的信息请见此处（https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html）。

除了Invitrogen alamarBlue 细胞活力检测试剂（Cell Viability Reagent）之外，AlgiMatrix 基质还能够与Invitrogen PrestoBlue 细胞活力检测试剂，Promega  Cell Titer-Glo 试剂或Invitrogen CyQuant  Direct Cell Proliferation Assay试剂盒相兼容。具体实验方案与2D细胞培养系统几乎相同，但需要加入纯AlgiMatrix 基质作为空白对照组。

This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye in it is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a non-cell-permeable quenching reagent in the kit which will both quench extracellular fluorescence (and thus this is a no-wash assay) AND will quench the fluorescence in dead cells (but not live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

This is true for MTT (as well as XTT, AlamarBlue Cell Viability Reagent, and PrestoBlue Cell Viability Reagent). CyQUANT Direct will also only count live cells, but for a different reason. The dye is a green-fluorescent nucleic acid stain, which will bind to DNA in all cells, live or dead, without the need for cellular metabolism. However, there is a cell impermeant quenching reagent in the kit which will quench both extracellular fluorescence (and thus this is a no-wash assay) and the fluorescence in dead cells (but not live cells).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

To make a 10X stock solution for the CyQUANT Direct Cell Proliferation Assay (Cat. No. C35011, C35012), we suggest using the following volumes:

1.1 mL         PBS
25 µL         CyQUANT Direct nucleic acid stain (Component A)
125 µL         CyQUANT Direct background suppressor I (Component B)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

No. The components of the CyQUANT Direct Red Cell Proliferation Assay and CyQuant Direct Cell Proliferation Assay kits are not interchangeable.

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The two kits utilize different dyes and background suppressors, but they function in the same way and provide similar results as can be seen from Figures 1 and 2 in the CyQUANT Direct Red Cell Proliferation Assay product sheet (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0018644_CyQUANT_direct_red_assay_PI.pdf). Dyes from both kits are nucleic acid stains and the background suppressors are cell impermeant reagents that quench extracellular fluorescence. They are usable within the same range (100 to 20,000 cells/well in a 96-well format) and in the same workflow.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

PrestoBlue Cell Viability Stain and CyQUANT Cell Proliferation Assay Kit can be used. We also offer a Neurite Outgrowth Staining Kit (Cat. No. A15001). More information about our different assays for neuronal cell health can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/neuroscience/neuronal-cell-health-assays.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Other than the Invitrogen alamarBlue Cell Viability Reagent, AlgiMatrix Matrix is also compatible with Invitrogen PrestoBlue Cell Viability Reagent and Invitrogen CyQuant Direct Cell Proliferation Assay. The protocol is almost the same as the 2D cell culture, but it needs the blank AlgiMatrix Matrix as a blank control.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.