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MegaX DH10B T1R Electrocomp™ Cells - FAQs

查看更多产品信息 MegaX DH10B T1R Electrocomp™ Cells - FAQs (C640003)

2 个常见问题解答

我正在构建酵母基因组文库，并希望利用你们的感受态进行转化和扩增。选择菌株时我应该关注哪些基因型特征？

我们推荐使用mcr/mrr–菌株，这种菌株可以防止甲基化的真核DNA在大肠杆菌宿主中的扩增受到限制。我们还推荐使用T1R菌株，因为T1是基因组/cDNA文库中一种常见的污染。

I'm making a yeast genomic library and want to transform and amplify it in one of your competent cell strains. What genotype features should I look for in choosing a good strain?

We would recommend a mcr/mrr- strain, which prevents restriction of methylated eukaryotic DNA in the E. coli host. We would also recommend using a T1R strain, as T1 is a common contaminant in genomic/cDNA libraries.