The use of the lipophilic fluorochrome CM-DiI for tracking the migration of lymphocytes.
AuthorsAndrade W, Seabrook TJ, Johnston MG, Hay JB
JournalJ Immunol Methods
PubMed ID8765171
'In this study we examined the new cell dye CM-DiI for tracking the migration of lymphocytes from blood to lymph. This lipophilic marker intercalates in the plasma membrane like the PKH dyes and older DiI derivatives. The stability and intensity of staining achieved with these dyes is better than most ... More
Characterization of antisera specific to NK1, NK2, and NK3 neurokinin receptors and their utilization to localize receptors in the rat gastrointestinal tract.
'Understanding the physiological role of tachykinins requires precise cellular and subcellular localization of their receptors. We raised antisera by immunizing rabbits with peptides corresponding to portions of the intracellular tails of the rat neurokinin 1, 2, and 3 receptors (NK1-R, NK2-R, NK3-R). Receptors were localized by immunofluorescence and confocal microscopy. ... More
Interneuron migration from basal forebrain to neocortex: dependence on Dlx genes.
AuthorsAnderson SA, Eisenstat DD, Shi L, Rubenstein JL
JournalScience
PubMed ID9334308
'Although previous analyses indicate that neocortical neurons originate from the cortical proliferative zone, evidence suggests that a subpopulation of neocortical interneurons originates within the subcortical telencephalon. For example, gamma-aminobutyric acid (GABA)-expressing cells migrate in vitro from the subcortical telencephalon into the neocortex. The number of GABA-expressing cells in neocortical slices ... More
Analysis of vertical fluorescence resonance energy transfer from the surface of a small-diameter sphere.
AuthorsJones GM, Wofsy C, Aurell C, Sklar LA
JournalBiophys J
PubMed ID9876165
'Fluorescence resonance energy transfer (FRET) measurements have been used to analyze fluorophore separations in a number of varying geometries, including small particles and extended surfaces. This study focuses on the geometry created by a donor extended above the surface of a small sphere (radius < R0), where the acceptors are ... More
Human mesenchymal stem cells exert potent antitumorigenic effects in a model of Kaposi's sarcoma.
AuthorsKhakoo AY, Pati S, Anderson SA, Reid W, Elshal MF, Rovira II, Nguyen AT, Malide D, Combs CA, Hall G, Zhang J, Raffeld M, Rogers TB, Stetler-Stevenson W, Frank JA, Reitz M, Finkel T,
JournalJ Exp Med
PubMed ID16636132
'Emerging evidence suggests that both human stem cells and mature stromal cells can play an important role in the development and growth of human malignancies. In contrast to these tumor-promoting properties, we observed that in an in vivo model of Kaposi''s sarcoma (KS), intravenously (i.v.) injected human mesenchymal stem cells ... More
Interaction of hepatitis C virus-like particles and cells: a model system for studying viral binding and entry.
AuthorsTriyatni M, Saunier B, Maruvada P, Davis AR, Ulianich L, Heller T, Patel A, Kohn LD, Liang TJ
JournalJ Virol
PubMed ID12186916
'Hepatitis C virus-like particles (HCV-LPs) containing the structural proteins of HCV H77 strain (1a genotype) was used as a model for HCV virion to study virus-cell interaction. HCV-LPs showed a buoyant density of 1.17 to 1.22 g/cm(3) in a sucrose gradient and formed double-shelled particles 35 to 49 nm in ... More
Fluorescent double-labeling with carbocyanine neuronal tracing and immunohistochemistry using a cholesterol-specific detergent digitonin.
AuthorsMatsubayashi Y, Iwai L, Kawasaki H,
JournalJ Neurosci Methods
PubMed ID18674563
'The fluorescent carbocyanine dye DiI (1,1''-dioctadecyl-3,3,3'',3''-tetramethylindocarbocyanine perchlorate) has been widely used for tracing of neuronal pathways. To examine identities of the DiI-labeled neuronal pathways, it is desirable to combine DiI labeling with immunofluorescent staining. However, DiI labeling and immunofluorescent staining are not well compatible, mainly because treatment of DiI-labeled neurons ... More
Embryonic origins of auditory brain-stem nuclei in the chick hindbrain.
AuthorsCramer KS, Fraser SE, Rubel EW
JournalDev Biol
PubMed ID10926755
'The auditory nuclei of the chick brain stem have distinct morphologies and highly specific synaptic connectivity. Nucleus magnocellularis (NM) and nucleus angularis receive tonotopically ordered cochlear input. NM in turn projects tonotopically to nucleus laminaris (NL), maintaining binaural specificity with projections to either dorsal or ventral NL dendrites. NM and ... More
Naturally occurring motoneuron cell death in rat upper respiratory tract motor nuclei: a histological, fast DiI and immunocytochemical study in the hypoglossal nucleus.
AuthorsFriedland DR, Eden AR, Laitman JT
JournalJ Neurobiol
PubMed ID7561831
'We have previously reported on our investigation of motoneuron cell death (MCD) in the rat nucleus ambiguus (NA). This article focuses on the other major upper respiratory tract motor nucleus: the hypoglossal. The hypoglossal nucleus (XII) contains motoneurons to the tongue and, as such, plays a critical role in defining ... More
Efferent and collateral organization of paratrigeminal nucleus projections: an anterograde and retrograde fluorescent tracer study in the rat.
AuthorsSaxon DW, Hopkins DA
JournalJ Comp Neurol
PubMed ID9831048
'The paratrigeminal nucleus (PTN) receives primary visceral afferent projections through cranial nerves IX and X and somatic afferent projections through cranial nerve V and dorsal roots as far caudally as C7. Pressure injections of the anterograde tracer tetramethylrhodamine dextran into the PTN in the rat resulted in bilateral labeling in ... More
Loss of binding and entry of liposome-DNA complexes decreases transfection efficiency in differentiated airway epithelial cells.
Authors Matsui H; Johnson L G; Randell S H; Boucher R C;
JournalJ Biol Chem
PubMed ID8995411
The target cells for gene therapy of cystic fibrosis lung disease are the well differentiated cells that line airway lumens. Employing cultures of airway epithelial cells that grow like ... More
DiOlistic Labeling of Neurons in Tissue Slices: A Qualitative and Quantitative Analysis of Methodological Variations.
AuthorsStaffend NA, Meisel RL,
JournalFront Neuroanat
PubMed ID21427781
Fine neuronal morphology, such as dendritic spines, classically has been studied using the Golgi technique; however, Golgi staining is difficult to combine with other histological techniques. With the increasing popularity of fluorescent imaging, a number of fluorescent dyes have been developed that enable the coupling of multiple fluorescent labels in ... More
Interaction of tau with the neural plasma membrane mediated by tau's amino-terminal projection domain.
AuthorsBrandt R, Léger J, Lee G
JournalJ Cell Biol
PubMed ID8522593
The neuronal microtubule-associated protein tau is required for the development of cell polarity in cultured neurons. Using PC12 cells that stably express tau and tau amino-terminal fragments, we report that tau interacts with the neural plasma membrane through its amino-terminal projection domain. In differentiated PC12 transfectants, tau is found in ... More
Tumor-targeted, systemic delivery of therapeutic viral vectors using hitchhiking on antigen-specific T cells.
AuthorsCole C, Qiao J, Kottke T, Diaz RM, Ahmed A, Sanchez-Perez L, Brunn G, Thompson J, Chester J, Vile RG,
JournalNat Med
PubMed ID16170322
Antigen-specific T cells circulate freely and accumulate specifically at sites of antigen expression. To enhance the survival and targeting of systemically delivered viral vectors, we exploited the observation that retroviral particles adhere nonspecifically, or 'hitchhike,' to the surface of T cells. Adoptive transfer of antigen-specific T cells, loaded with viruses ... More
Application and limitations of chloromethyl-benzamidodialkylcarbocyanine for tracing cells used in bone Tissue engineering.
AuthorsKruyt MC, De Bruijn J, Veenhof M, Oner FC, Van Blitterswijk CA, Verbout AJ, Dhert WJ,
JournalTissue Eng
PubMed ID12625959
Bone tissue engineering has the potential to provide us with an autologous bone substitute. Despite extensive research to optimize the technique, little is known about the survival and function of the cells after implantation. To monitor the cells, in vivo labeling is the method of choice. In this study we ... More
Mesenchymal stem cells: isolation, characterisation and in vivo fluorescent dye tracking.
Cell therapies have been used to regenerate the heart by direct myocardial delivery, by coronary infusion and by surface attached scaffolds. Multipotent mesenchymal stem cells (MSC) with capacity to differentiate into cardiomyocytes and other cell lines have been predominantly trialled in rodents. However, large animal models are increasingly needed to ... More
Combination of diOlistic labeling with retrograde tract tracing and immunohistochemistry.
AuthorsNeely MD, Stanwood GD, Deutch AY,
JournalJ Neurosci Methods
PubMed ID19712695
Neuronal staining techniques have played a crucial role in the analysis of neuronal function. Several different staining techniques have been developed to allow morphological analyses of neurons. DiOlistic labeling, in which beads are coated with a lipophilic dye and then ballistically ejected onto brain tissue, has recently been introduced as ... More
Suitability of various membrane lipophilic probes for the detection of trogocytosis by flow cytometry.
AuthorsDaubeuf S, Bordier C, Hudrisier D, Joly E,
JournalCytometry A
PubMed ID19051238
Trogocytosis is a recently discovered phenomenon whereby lymphocytes capture fragments of the plasma membrane from antigen presenting cells (APCs). Using APCs labeled with widely used fluorescent lipophilic probes, we previously described a trogocytosis analysis protocol (TRAP) useful to understand the mechanisms and biological consequences of this process and to identify ... More
Deoxycholate promotes survival of breast cancer cells by reducing the level of pro-apoptotic ceramide.
AuthorsKrishnamurthy K, Wang G, Rokhfeld D, Bieberich E,
JournalBreast Cancer Res
PubMed ID19087284
INTRODUCTION: At physiologic concentration in serum, the bile acid sodium deoxycholate (DC) induces survival and migration of breast cancer cells. Here we provide evidence of a novel mechanism by which DC reduces apoptosis that is induced by the sphingolipid ceramide in breast cancer cells. METHODS: Murine mammacarcinoma 4T1 cells were ... More
Flow cytometric determination of endocytosis of viable labelled Legionella pneumophila by Acanthamoeba palestinensis.
AuthorsHarf C, Goffinet S, Meunier O, Monteil H, Colin DA
JournalCytometry
PubMed ID9041116
Endocytosis of fluorescently labelled cells of Legionella pneumophila (L. pneumophila) by free-living Acanthamoeba palestinensis (A. palestinensis) has been studied using flow cytometry. L. pneumophila cells were labelled with CM-DiI, a lipophilic fluorescent probe under conditions that did not modify viability. Coculturing the bacteria with amoebae was accompanied by rapid endocytosis; ... More
Dectin-1 is required for host defense against Pneumocystis carinii but not against Candida albicans.
AuthorsSaijo S, Fujikado N, Furuta T, Chung SH, Kotaki H, Seki K, Sudo K, Akira S, Adachi Y, Ohno N, Kinjo T, Nakamura K, Kawakami K, Iwakura Y
JournalNat Immunol
PubMed ID17159982
Dectin-1 is a C-type lectin involved in the recognition of beta-glucans found in the cell walls of fungi. We generated dectin-1-deficient mice to determine the importance of dectin-1 in the defense against pathogenic fungi. In vitro, beta-glucan-induced cytokine production from wild-type dendritic cells and macrophages was abolished in cells homozygous ... More
Isolation of neural stem cells from the postnatal cerebellum.
AuthorsLee A, Kessler JD, Read TA, Kaiser C, Corbeil D, Huttner WB, Johnson JE, Wechsler-Reya RJ
JournalNat Neurosci
PubMed ID15908947
The cerebellum is critical for motor coordination and cognitive function and is the target of transformation in medulloblastoma, the most common malignant brain tumor in children. Although the development of granule cells, the most abundant neurons in the cerebellum, has been studied in detail, the origins of other cerebellar neurons ... More
Cathepsin L is required for endothelial progenitor cell-induced neovascularization.
AuthorsUrbich C, Heeschen C, Aicher A, Sasaki K, Bruhl T, Farhadi MR, Vajkoczy P, Hofmann WK, Peters C, Pennacchio LA, Abolmaali ND, Chavakis E, Reinheckel T, Zeiher AM, Dimmeler S
JournalNat Med
PubMed ID15665831
Infusion of endothelial progenitor cells (EPC), but not of mature endothelial cells, promotes neovascularization after ischemia. We performed gene expression profiling of EPC and endothelial cells to identify genes that might be important for the neovascularization capacity of EPC. Notably, the protease cathepsin L (CathL) was highly expressed in EPC ... More
Functional small-diameter neovessels created using endothelial progenitor cells expanded ex vivo.
AuthorsKaushal S, Amiel GE, Guleserian KJ, Shapira OM, Perry T, Sutherland FW, Rabkin E, Moran AM, Schoen FJ, Atala A, Soker S, Bischoff J, Mayer JE
JournalNat Med
PubMed ID11533707
Arterial conduits are increasingly preferred for surgical bypass because of inherent functional properties conferred by arterial endothelial cells, especially nitric oxide production in response to physiologic stimuli. Here we tested whether endothelial progenitor cells (EPCs) can replace arterial endothelial cells and promote patency in tissue-engineered small-diameter blood vessels (4 mm). ... More
Block copolymers modify the internalization of micelle-incorporated probes into neural cells.
AuthorsMaysinger D, Berezovska O, Savic R, Soo PL, Eisenberg A
JournalBiochim Biophys Acta
PubMed ID11420118
An important therapeutic concern is rate and extent of internalization of drugs into cells. Hydrophilic agents often internalize poorly and slowly, and highly lipophilic ones too rapidly. The incorporation of drugs into micelles allows regulation of their internalization parameters, and newly-described block copolymers can be selectively tailored to suit specific ... More
In vivo tracking of bone marrow fibroblasts with fluorescent carbocyanine dye.
AuthorsFerrari A, Hannouche D, Oudina K, Bourguignon M, Meunier A, Sedel L, Petite H
JournalJ Biomed Mater Res
PubMed ID11372053
Recent advances in the field of tissue engineering have culminated in new tissue substitutes that combine a biomaterial and precursor cells. The effectiveness of these materials is generally assessed in animals, but few studies explore the fate of the transplanted cells in vivo, despite its paramount importance for understanding the ... More
Protective immunity against atherosclerosis carried by B cells of hypercholesterolemic mice.
AuthorsCaligiuri G, Nicoletti A, Poirier B, Hansson GK
JournalJ Clin Invest
PubMed ID11901183
Atherosclerosis is characterized by vascular inflammation and associated with systemic and local immune responses to oxidized LDL (oxLDL) and other antigens. Since immunization with oxLDL reduces atherosclerosis, we hypothesized that the disease might be associated with development of protective immunity. Here we show that spleen-associated immune activity protects against atherosclerosis. ... More
The relationship of blood lymphocytes to the recirculating lymphocyte pool.
AuthorsAndrade WN, Johnston MG, Hay JB
JournalBlood
PubMed ID9473231
Lymphocyte recirculation facilitates the detection and elimination of pathogens and the dissemination of immunologic memory. It is generally assumed that all small lymphocytes in the blood are actively recirculating, yet there is little quantitative data directly comparing the migration of this population with actively recirculating, lymph-derived lymphocytes. In this study ... More
Transplanted fetal fibroblasts: survival and distribution over time in normal adult dermis compared with autogenic, allogenic, and xenogenic adult fibroblasts.
AuthorsHebda PA, Dohar JE
JournalOtolaryngol Head Neck Surg
PubMed ID10471865
Cell therapy is a widely applicable therapeutic approach using cells and cell elements, frequently from fetal or young animals, for their beneficial effects. This study evaluated the host response to and tolerance of transplanted fetal skin fibroblasts. Cultured fibroblasts from adult rabbit skin (autogenic and allogenic), 21-day fetal rabbit skin ... More
Substance P-induced trafficking of beta-arrestins. The role of beta-arrestins in endocytosis of the neurokinin-1 receptor.
AuthorsMcConalogue K, Déry O, Lovett M, Wong H, Walsh JH, Grady EF, Bunnett NW
JournalJ Biol Chem
PubMed ID10347182
Agonist-induced redistribution of G-protein-coupled receptors (GPCRs) and beta-arrestins determines the subsequent cellular responsiveness to agonists and is important for signal transduction. We examined substance P (SP)-induced trafficking of beta-arrestin1 and the neurokinin-1 receptor (NK1R) in KNRK cells in real time using green fluorescent protein. Green fluorescent protein did not alter ... More
Elimination of plasma membrane phosphatidylinositol (4,5)-bisphosphate is required for exocytosis from mast cells.
AuthorsHammond GR, Dove SK, Nicol A, Pinxteren JA, Zicha D, Schiavo G
JournalJ Cell Sci
PubMed ID16687737
The inositol lipid phosphatidylinositol (4,5)-bisphosphate [PtdIns(4,5)P2] is involved in a myriad of cellular processes, including the regulation of exocytosis and endocytosis. In this paper, we address the role of PtdIns(4,5)P2 in compound exocytosis from rat peritoneal mast cells. This process involves granule-plasma membrane fusion as well as homotypic granule membrane ... More
Impact of transplanted fibroblasts on rabbit skin wounds.
AuthorsSandulache VC, Zhou Z, Sherman A, Dohar JE, Hebda PA
JournalArch Otolaryngol Head Neck Surg
PubMed ID12622547
OBJECTIVES: To determine the effect of injected fibroblasts on full-thickness cutaneous wounds and to distinguish between the properties of fetal vs adult fibroblasts. DESIGN: Full-thickness cutaneous wounds were created by incision in the skin of adult New Zealand white rabbits and treated with fluorescently labeled autogenic, allogenic, and xenogenic fetal ... More
Distinct modes of macrophage recognition for apoptotic and necrotic cells are not specified exclusively by phosphatidylserine exposure.
AuthorsCocco RE, Ucker DS
JournalMol Biol Cell
PubMed ID11294896
The distinction between physiological (apoptotic) and pathological (necrotic) cell deaths reflects mechanistic differences in cellular disintegration and is of functional significance with respect to the outcomes that are triggered by the cell corpses. Mechanistically, apoptotic cells die via an active and ordered pathway; necrotic deaths, conversely, are chaotic and passive. ... More
Dendritic cells internalize vaccine adjuvant after intramuscular injection.
AuthorsDupuis M, Murphy TJ, Higgins D, Ugozzoli M, van Nest G, Ott G, McDonald DM
JournalCell Immunol
PubMed ID9637761
Vaccine adjuvants help antigens elicit rapid, potent, and long-lasting immune responses. The lack of understanding of the immunological mechanism of action of adjuvants has limited the rational development of vaccines for human use. In particular, little is known about how the immune system processes adjuvants. The goal of the present ... More
Coherent development of dermomyotome and dermis from the entire mediolateral extent of the dorsal somite.
AuthorsBen-Yair R, Kahane N, Kalcheim C
JournalDevelopment
PubMed ID12900449
We have previously shown that overall growth of the myotome in the mediolateral direction occurs in a coherent and uniform pattern. We asked whether development of the dermomyotome and resultant dermis follow a similar pattern or are, alternatively, controlled by restricted pools of stem cells driving directional growth. To this ... More
Reciprocal expression of the TNF family receptor herpes virus entry mediator and its ligand LIGHT on activated T cells: LIGHT down-regulates its own receptor.
AuthorsMorel Y, Schiano de Colella JM, Harrop J, Deen KC, Holmes SD, Wattam TA, Khandekar SS, Truneh A, Sweet RW, Gastaut JA, Olive D, Costello RT
JournalJ Immunol
PubMed ID11035077
The TNF receptor (TNFR) family plays a central role in the development of the immune response. Here we describe the reciprocal regulation of the recently identified TNFR superfamily member herpes virus entry mediator (HVEM) (TR2) and its ligand LIGHT (TL4) on T cells following activation and the mechanism of this ... More
Naturally occurring motoneuron cell death in rat upper respiratory tract motor nuclei: a histological, fast DiI and immunocytochemical study of the nucleus ambiguus.
AuthorsFriedland DR, Eden AR, Laitman JT
JournalJ Neurobiol
PubMed ID7602319
The mammalian upper respiratory tract (URT) serves as the common modality for aspects of respiration, deglutition, and vocalization. Although these actions are dependent on coordinated and specific neuromuscular control, little is known about the development of URT control centers. As such, this study investigated the occurrence of naturally occurring motoneuron ... More
Phosphatidylinositol 4,5-bisphosphate induces actin-based movement of raft-enriched vesicles through WASP-Arp2/3.
AuthorsRozelle AL, Machesky LM, Yamamoto M, Driessens MH, Insall RH, Roth MG, Luby-Phelps K, Marriott G, Hall A, Yin HL
JournalCurr Biol
PubMed ID10744973
BACKGROUND: Phosphatidylinositol 4,5-bisphosphate (PIP(2)) has been implicated in the regulation of the actin cytoskeleton and vesicle trafficking. It stimulates de novo actin polymerization by activating the pathway involving the Wiskott-Aldrich syndrome protein (WASP) and the actin-related protein complex Arp2/3. Other studies show that actin polymerizes from cholesterol-sphingolipid-rich membrane microdomains called ... More
Cell-cell adhesion in human fibroblasts requires calcium signaling.
AuthorsKo KS, Arora PD, Bhide V, Chen A, McCulloch CA
JournalJ Cell Sci
PubMed ID11228159
In connective tissues, intercellular adhesion is essential for tissue morphogenesis, development and wound healing. However, the signaling mechanisms initiated by cell-cell adhesion in fibroblasts and that regulate it are not known. In this study we tested the hypothesis that intracellular calcium signaling is required to mediate intercellular adhesion between fibroblasts. ... More
Role of Dicer and Drosha for endothelial microRNA expression and angiogenesis.
AuthorsKuehbacher A, Urbich C, Zeiher AM, Dimmeler S
JournalCirc Res
PubMed ID17540974
MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression by binding to the cellular transcript leading to translational repression or degradation of the target mRNA. Dicer and Drosha are the miRNA processing enzymes that are required for the maturation of miRNAs. Here, we investigated the role of Dicer and ... More
Cationic albumin-conjugated pegylated nanoparticles allow gene delivery into brain tumors via intravenous administration.
AuthorsLu W, Sun Q, Wan J, She Z, Jiang XG
JournalCancer Res
PubMed ID17178885
Patients with malignant gliomas have a poor prognosis because these tumors do not respond well to conventional treatments. Studies of glioma xenografts suggest that they may be amenable to gene therapy with cytotoxic genes, such as the proapoptotic Apo2 ligand/tumor necrosis factor-related apoptosis-inducing ligand (Apo2L/TRAIL). Gene therapy of gliomas ideally ... More
Organ-specific endothelial cell uptake of cationic liposome-DNA complexes in mice.
AuthorsMcLean JW, Fox EA, Baluk P, Bolton PB, Haskell A, Pearlman R, Thurston G, Umemoto EY, McDonald DM
JournalAm J Physiol
PubMed ID9249514
This study identified the organ and cellular distribution of cationic liposome-DNA complexes injected intravenously into CD-1 mice for gene delivery. DOTIM-cholesterol liposomes were labeled with the fluorescent dye CM-Dil and complexed with plasmid DNA encoding the chloramphenicol acetyltransferase reporter gene. The distribution of the complexes was examined in 29 organs ... More
Oxidant generation by single infected monocytes after short-term fluorescence labeling of a protozoan parasite.
AuthorsChang HK, Thalhofer C, Duerkop BA, Mehling JS, Verma S, Gollob KJ, Almeida R, Wilson ME
JournalInfect Immun
PubMed ID17118986
Leishmania spp. are intracellular protozoa residing in mononuclear phagocytes. Leishmania organisms are susceptible to microbicidal responses generated in response to phagocytosis. Assuming that both phagocyte and parasite populations are heterogeneous, it is advantageous to examine the response of individual cells phagocytosing living parasites. Because Leishmania spp. lose virulence during the ... More
The development of the trunk neural crest in the turtle Trachemys scripta.
AuthorsGoldberg S, Venkatesh A, Martinez J, Dombroski C, Abesamis J, Campbell C, Mccalipp M, de Bellard ME
JournalDev Dyn
PubMed ID31587387
'The neural crest is a group of multipotent cells that give rise to a wide variety of cells, especially portion of the peripheral nervous system. Neural crest cells (NCCs) show evolutionary conserved fate restrictions based on their axial level of origin: cranial, vagal, trunk, and sacral. While much is known ... More
Simvastatin Attenuates Cardiac Fibrosis via Regulation of Cardiomyocyte-Derived Exosome Secretion.
AuthorsKuo HF, Hsieh CC, Wang SC, Chang CY, Hung CH, Kuo PL, Liu YR, Li CY, Liu PL
JournalJ Clin Med
PubMed ID31167519
Exosome-mediated communication within the cardiac microenvironment is associated with cardiac fibrosis. Simvastatin (SIM), a potent statin, protects against cardiac fibrosis, but its mechanism of action is unclear. We investigated the inhibitory effects and underlying mechanism of simvastatin in cardiac fibrosis, by regulating exosome-mediated communication. Male Sprague-Dawley rats were treated with ... More
Spinal neural tube closure depends on regulation of surface ectoderm identity and biomechanics by Grhl2.
AuthorsNikolopoulou E, Hirst CS, Galea G, Venturini C, Moulding D, Marshall AR, Rolo A, De Castro SCP, Copp AJ, Greene NDE
JournalNat Commun
PubMed ID31171776
Lack or excess expression of the surface ectoderm-expressed transcription factor Grainyhead-like2 (Grhl2), each prevent spinal neural tube closure. Here we investigate the causative mechanisms and find reciprocal dysregulation of epithelial genes, cell junction components and actomyosin properties in Grhl2 null and over-expressing embryos. Grhl2 null surface ectoderm shows a shift ... More
MiR-219a-5p Enriched Extracellular Vesicles Induce OPC Differentiation and EAE Improvement More Efficiently Than Liposomes and Polymeric Nanoparticles.
AuthorsOsorio-Querejeta I, Carregal-Romero S, Ayerdi-Izquierdo A, Mäger I, A NL, Wood M, Egimendia A, Betanzos M, Alberro A, Iparraguirre L, Moles L, Llarena I, Möller M, Goñi-de-Cerio F, Bijelic G, Ramos-Cabrer P, Muñoz-Culla M, Otaegui D
JournalPharmaceutics
PubMed ID32098213
Remyelination is a key aspect in multiple sclerosis pathology and a special effort is being made to promote it. However, there is still no available treatment to regenerate myelin and several strategies are being scrutinized. Myelination is naturally performed by oligodendrocytes and microRNAs have been postulated as a promising tool ... More
Reinforced alginate/gelatin sponges functionalized by avidin/biotin-binding strategy: a novel cardiac patch.
AuthorsFrati C, Graiani G, Barbani N, Madeddu D, Falco A, Quaini F, Lazzeri L, Cascone MG, Rosellini E