Condensation of plasmid DNA with polylysine improves liposome-mediated gene transfer into established and primary muscle cells.
AuthorsVitiello L, Chonn A, Wasserman JD, Duff C, Worton RG
JournalGene Ther
PubMed ID9156800
'Cationic liposomes provide a means to introduce genes into cells both ex vivo and in vivo. In the past few years their use has been described in several tissues, e.g. lungs, liver, endothelium, brain. In this study we evaluated a commercially available poly-cationic liposome formulation in delivering a reporter gene ... More
Inheritance of a pre-inactivated paternal X chromosome in early mouse embryos.
AuthorsHuynh KD, Lee JT
JournalNature
PubMed ID14661031
'In mammals, dosage compensation ensures equal X-chromosome expression between males (XY) and females (XX) by transcriptionally silencing one X chromosome in XX embryos. In the prevailing view, the XX zygote inherits two active X chromosomes, one each from the mother and father, and X inactivation does not occur until after ... More
Substantial background reduction in ligase-based apoptosis detection using newly designed hairpin oligonucleotide probes.
AuthorsDidenko VV, Boudreaux DJ, Baskin DS
JournalBiotechniques
PubMed ID10631490
DNA methylation promotes Aurora-B-driven phosphorylation of histone H3 in chromosomal subdomains.
AuthorsMonier K, Mouradian S, Sullivan KF,
JournalJ Cell Sci
PubMed ID17164288
Confinement of enzymatic reactions to nuclear and chromosomal subdomains regulates functional organization of the nucleus. Aurora-B kinase regulates cell-cycle-dependent phosphorylation of chromosomal substrates through sequential localization to a series of sites on chromosomes and the mitotic spindle. In G2 nuclei, Aurora-B recruitment to heterochromatin restricts histone H3S10 phosphorylation to a ... More
Spectral karyotyping combined with locus-specific FISH simultaneously defines genes and chromosomes involved in chromosomal translocations.
AuthorsTonon G, Roschke A, Stover K, Shou Y, Kuehl WM, Kirsch IR
JournalGenes Chromosomes Cancer
PubMed ID10719373
Genes that play roles in malignant transformation have often been found proximate to cancer-associated chromosomal breakpoints. Identifying genes that flank chromosomal reconfigurations is thus essential for cancer cytogenetics. To simplify and expedite this identification, we have developed a novel approach, based on simultaneous spectral karyotyping and fluorescence in situ hybridization ... More
Presence of double-strand breaks with single-base 3' overhangs in cells undergoing apoptosis but not necrosis.
AuthorsDidenko VV, Hornsby PJ
JournalJ Cell Biol
PubMed ID8947557
Apoptotic cells in rat thymus were labeled in situ in paraffin-embedded and frozen tissue sections by ligation of double-stranded DNA fragments containing digoxigenin or Texas red. Two forms of double-stranded DNA fragments were prepared using the polymerase chain reaction: one was synthesized using Taq polymerase, which yields products with single-base ... More
Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping.
AuthorsMacville M, Schröck E, Padilla-Nash H, Keck C, Ghadimi BM, Zimonjic D, Popescu N, Ried T
JournalCancer Res
PubMed ID9892199
We revisited the cytogenetic alterations of the cervical adenocarcinoma cell line HeLa through the use of spectral karyotyping (SKY), comparative genomic hybridization (CGH), and fluorescence in situ hybridization (FISH). SKY analysis unequivocally characterized all abnormal chromosomes. Chromosomal breakpoints were primarily assigned by simultaneous assessment of SKY painted chromosomes and inverted ... More
Hidden chromosome abnormalities in haematological malignancies detected by multicolour spectral karyotyping.
AuthorsVeldman T, Vignon C, Schröck E, Rowley JD, Ried T
JournalNat Genet
PubMed ID9090389
Cytogenetic analysis provides critical information of diagnostic and prognostic importance for haematological malignancies. In fact, the identification of recurring chromosomal breakpoints in leukaemias and lymphomas has expedited the cloning of genes whose translocation-induced deregulation causes malignant transformation. The pillar of karyotype analysis rests on chromosome banding techniques that have the ... More