Lipofectamine™ CRISPRMAX™ Cas9 转染试剂
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Lipofectamine™ CRISPRMAX™ Cas9 转染试剂
Invitrogen™

Lipofectamine™ CRISPRMAX™ Cas9 转染试剂

Lipofectamine CRISPRMAX Cas9 转染试剂是第一种用于 CRISPR-Cas9 蛋白递送的优化脂质体纳米微粒转染试剂。这是电穿孔的适用于高通量、成本节约的替代品。TrueCut Cas9了解更多信息
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货号数量
CMAX000030.3 mL
CMAX000080.75 mL
CMAX000010.1 mL
CMAX000151.5 mL
货号 CMAX00003
价格(CNY)
3,443.21
飞享价
Ends: 31-Dec-2025
4,124.00
共减 680.79 (17%)
Each
添加至购物车
数量:
0.3 mL
价格(CNY)
3,443.21
飞享价
Ends: 31-Dec-2025
4,124.00
共减 680.79 (17%)
Each
添加至购物车
Lipofectamine CRISPRMAX Cas9 转染试剂是第一种用于 CRISPR-Cas9 蛋白递送的优化脂质体纳米微粒转染试剂。这是电穿孔的适用于高通量、成本节约的替代品。

TrueCut Cas9 蛋白 v2TrueGuide 合成 gRNA 结合使用时,其提供:
•显示切割效率—在超过 20 个细胞类型中检测,包括 iPSC、mESC、N2A、CHO、A549、HCT116、HeLa、HEK293 和几种其他类型
•低细胞毒性—减少启动实验所需的细胞
•适用于高通量— 96 孔形式的理想递送解决方案
•节约成本—无论是单反应成本还是初始投资

在新型基因组编辑应用中实现高转染效率
Lipofectamine CRISPRMAX 转染试剂增加成功切割和重组的可能性,特别是当与我们的 TrueCut Cas9 蛋白 v2 和 TrueGuide 合成 gRNA 结合使用时。这最终将性能基因修饰的效率最大化。

使用 TrueCut Cas9 蛋白 v2 改善实验结果
与 CRISPR 质粒或 Cas9 mRNA 不同,在原代细胞和干细胞中使用 Cas9 基因提供优越的切割效率。其消除了对有效载荷的转录或翻译的需求,消除基因组整合风险,并且独立于细胞周期。
仅供科研使用。不可用于诊断程序。
规格
分类无动物来源
适用于(应用)转染
产品线CRISPRMAX™、Lipofectamine™
产品类型转染试剂
数量0.3 mL
血清兼容性
运输条件经批准可在室温下或置于湿冰上运输
细胞类型已建立的细胞系, 干细胞, 原代细胞, 难转染细胞
产品规格6孔板、12孔板、24孔板、48孔板、96孔板、培养瓶
样品类型蛋白, 核糖核蛋白, Cas9 核糖核蛋白, Cas9, 质粒
转染技术脂质转染
Unit SizeEach
内容与储存
含有一小瓶 Lipofectamine CRISPRMAX 试剂和一小瓶 Cas9 Plus 试剂。

在 2–8°C 下储存。切勿冷冻。

常见问题解答 (FAQ)

Can I use Lipofectamine CRISPRMAX Cas9 Transfection Reagent with one-cell embryos?

We have no data regarding the use of CRISPRMAX in one-celled embryos, therefore we do not recommend using this product for this application.

The shipping for my Lipofectamine CRISPRMAX Cas9 Transfection Reagent was delayed. Can I still use it?

All of the Lipofectamine reagents, including Lipofectamine CRISPRMAX Cas9 Transfection Reagent, are stable at room temperature for months. What is most important is that the reagent did not freeze, because that will alter the integrity and composition of the lipid particles and will compromise usability. Previous freezing can be evidenced by precipitation and excessive cloudiness of the reagent. A limited amount of cloudiness is normal, but excessive cloudiness is a sign that the reagent froze at some point. If the reagent you received looks clear or a bit cloudy the product should still be usable. Some additional data on room temperature stability of some Lipofectamine reagents, in the context of shipping, can be found in this white paper.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

How much Lipofectamine CRISPRMAX Reagent, Cas9 protein, and gRNA should I complex for gene editing?

Please see the Lipofectamine CRISPRMAX Reagent manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0014545_lipofectamine_crispermax_QR.pdf) for protocol details or contact techsupport@thermofisher.com.

How does the Lipofectamine CRISPRMAX Reagent work?

The Lipofectamine CRISPRMAX Reagent combined with the proprietary enhancement properties of the Lipofectamine Cas9 Plus Reagent leads to efficient complex formation with the Cas9-gRNA ribonucleoprotein, for best delivery to the nucleus, helping to ensure high gene editing frequency for a wide range of cell types.

Can the Lipofectamine CRISPRMAX Reagent be used to deliver proteins other than the Cas9 nuclease?

Although the Lipofectamine CRISPRMAX Reagent was developed for the delivery of the Cas9-gRNA ribonucleoprotein, there is potential for other protein complex applications.