Lipofectamine™ CRISPRMAX™ Cas9 Transfection Reagent, 0.3 mL - FAQs

We have no data regarding the use of CRISPRMAX in one-celled embryos, therefore we do not recommend using this product for this application.

All of the Lipofectamine reagents, including Lipofectamine CRISPRMAX Cas9 Transfection Reagent, are stable at room temperature for months. What is most important is that the reagent did not freeze, because that will alter the integrity and composition of the lipid particles and will compromise usability. Previous freezing can be evidenced by precipitation and excessive cloudiness of the reagent. A limited amount of cloudiness is normal, but excessive cloudiness is a sign that the reagent froze at some point. If the reagent you received looks clear or a bit cloudy the product should still be usable. Some additional data on room temperature stability of some Lipofectamine reagents, in the context of shipping, can be found in this white paper.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Please see the Lipofectamine CRISPRMAX Reagent manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0014545_lipofectamine_crispermax_QR.pdf) for protocol details or contact techsupport@thermofisher.com.

The Lipofectamine CRISPRMAX Reagent combined with the proprietary enhancement properties of the Lipofectamine Cas9 Plus Reagent leads to efficient complex formation with the Cas9-gRNA ribonucleoprotein, for best delivery to the nucleus, helping to ensure high gene editing frequency for a wide range of cell types.

Although the Lipofectamine CRISPRMAX Reagent was developed for the delivery of the Cas9-gRNA ribonucleoprotein, there is potential for other protein complex applications.

The Lipofectamine CRISPRMAX Reagent was developed to efficiently deliver the Cas9-gRNA ribonucleoprotein complex. We recommend that you first deliver the donor plasmid with Lipofectamine 3000, then follow with delivering the Cas9-gRNA complex with Lipofectamine CRISPRMAX Reagent for best editing efficiency.