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引用和文献 (18)
Invitrogen™
DQ™ 人类胎盘 IV 型胶原，荧光素偶联物
荧光 DQ™ 胶原可用于直接监测胶原酶活性。DQ™ 底物是天然底物的类似物，其与过量荧光染料相连，因此几乎不存在荧光信号。这种信号的淬灭是由于完整底物上的染料紧密靠近造成的。酶驱动的底物水解导致染料分离，使染料分子彼此分离并且荧光信号增强。了解更多信息
| 货号 | 数量 |
|---|---|
| D12052 | 1 mg |
货号 D12052
价格（CNY)
5,445.00
Each
数量:
1 mg
价格（CNY)
5,445.00
Each
荧光 DQ™ 胶原可用于直接监测胶原酶活性。DQ™ 底物是天然底物的类似物，其与过量荧光染料相连，因此几乎不存在荧光信号。这种信号的淬灭是由于完整底物上的染料紧密靠近造成的。酶驱动的底物水解导致染料分离，使染料分子彼此分离并且荧光信号增强。
仅供科研使用。不可用于诊断程序。
规格
产品线DQ
数量1 mg
运输条件室温
底物蛋白酶底物
检测方法荧光
形式冻干
底物属性基于蛋白的底物
目标酶金属蛋白酶
Unit SizeEach
内容与储存
在冷冻冰箱（-5 至 -30°C）中避光储存。
引用和文献 (18)
引用和文献
Abstract
Intracellular and extracellular cathepsin B facilitate invasion of MCF-10A neoT cells through reconstituted extracellular matrix in vitro.
Journal:Exp Cell Res
PubMed ID:12581740
'Lysosomal cysteine proteinase cathepsin B is implicated in remodeling the extracellular matrix, a crucial step in the process of tumor cell invasion. In this study the contributions of intracellular and extracellular cathepsin B activities in the invasion of ras-transformed human breast epithelial cells, MCF-10A neoT, were assessed using specific cathepsin
Matrix metalloproteinase activity in urine of patients with renal cell carcinoma leads to degradation of extracellular matrix proteins: possible use as a screening assay.
Journal:J Urol
PubMed ID:12629409
'PURPOSE: Localized renal cell carcinoma is usually curable by nephrectomy. However, a large fraction of patients already present with metastatic disease, which results in a poor outcome. Currently no clinically relevant screening assay is available to detect early stage renal cell carcinoma. We investigated whether urinary extracellular matrix (ECM) proteins
Imaging proteolysis by living human glioma cells.
Journal:Biol Chem
PubMed ID:11517931
'Degradation of basement membrane is an essential step for tumor invasion. In order to study degradation in real time as well as localize the site of proteolysis, we have established an assay with living human cancer cells in which we image cleavage of quenched-fluorescent basement membrane type IV collagen (DQ-collagen
Metabolic mapping of proteinase activity with emphasis on in situ zymography of gelatinases: review and protocols.
Journal:J Histochem Cytochem
PubMed ID:15150280
'Proteases are essential for protein catabolism, regulation of a wide range of biological processes, and in the pathogenesis of many diseases. Several techniques are available to localize activity of proteases in tissue sections or cell preparations. For localization of the activity of matrix metalloproteinases, in situ zymography was introduced some
Functional imaging of proteolysis: stromal and inflammatory cells increase tumor proteolysis.
Journal:Mol Imaging
PubMed ID:14649059
'The underlying basement membrane is degraded during progression of breast and colon carcinoma. Thus, we imaged degradation of a quenched fluorescent derivative of basement membrane type IV collagen (DQ-collagen IV) by living human breast and colon tumor spheroids. Proteolysis of DQ-collagen IV by HCT 116 and HKh-2 human colon tumor