Intracellular and extracellular cathepsin B facilitate invasion of MCF-10A neoT cells through reconstituted extracellular matrix in vitro.
AuthorsPremzl A, Zavasnik-Bergant V, Turk V, Kos J
JournalExp Cell Res
PubMed ID12581740
'Lysosomal cysteine proteinase cathepsin B is implicated in remodeling the extracellular matrix, a crucial step in the process of tumor cell invasion. In this study the contributions of intracellular and extracellular cathepsin B activities in the invasion of ras-transformed human breast epithelial cells, MCF-10A neoT, were assessed using specific cathepsin ... More
Matrix metalloproteinase activity in urine of patients with renal cell carcinoma leads to degradation of extracellular matrix proteins: possible use as a screening assay.
AuthorsSherief MH, Low SH, Miura M, Kudo N, Novick AC, Weimbs T
JournalJ Urol
PubMed ID12629409
'PURPOSE: Localized renal cell carcinoma is usually curable by nephrectomy. However, a large fraction of patients already present with metastatic disease, which results in a poor outcome. Currently no clinically relevant screening assay is available to detect early stage renal cell carcinoma. We investigated whether urinary extracellular matrix (ECM) proteins ... More
Imaging proteolysis by living human glioma cells.
AuthorsSameni M, Dosescu J, Sloane BF
JournalBiol Chem
PubMed ID11517931
'Degradation of basement membrane is an essential step for tumor invasion. In order to study degradation in real time as well as localize the site of proteolysis, we have established an assay with living human cancer cells in which we image cleavage of quenched-fluorescent basement membrane type IV collagen (DQ-collagen ... More
Metabolic mapping of proteinase activity with emphasis on in situ zymography of gelatinases: review and protocols.
AuthorsFrederiks WM, Mook OR
JournalJ Histochem Cytochem
PubMed ID15150280
'Proteases are essential for protein catabolism, regulation of a wide range of biological processes, and in the pathogenesis of many diseases. Several techniques are available to localize activity of proteases in tissue sections or cell preparations. For localization of the activity of matrix metalloproteinases, in situ zymography was introduced some ... More
Functional imaging of proteolysis: stromal and inflammatory cells increase tumor proteolysis.
AuthorsSameni M, Dosescu J, Moin K, Sloane BF
JournalMol Imaging
PubMed ID14649059
'The underlying basement membrane is degraded during progression of breast and colon carcinoma. Thus, we imaged degradation of a quenched fluorescent derivative of basement membrane type IV collagen (DQ-collagen IV) by living human breast and colon tumor spheroids. Proteolysis of DQ-collagen IV by HCT 116 and HKh-2 human colon tumor ... More
Functional imaging of tumor proteolysis.
AuthorsSloane BF, Sameni M, Podgorski I, Cavallo-Medved D, Moin K
JournalAnnu Rev Pharmacol Toxicol
PubMed ID16402907
'The roles of proteases in cancer are now known to be much broader than simply degradation of extracellular matrix during tumor invasion and metastasis. Furthermore, proteases from tumor-associated cells (e.g., fibroblasts, inflammatory cells, endothelial cells) as well as tumor cells are recognized to contribute to pathways critical to neoplastic progression. ... More
Imaging and quantifying the dynamics of tumor-associated proteolysis.
The roles of proteases in cancer are dynamic. Furthermore, the roles or functions of any one protease may differ from one stage of cancer to another. Proteases from tumor-associated cells (e.g., fibroblasts, inflammatory cells, endothelial cells) as well as from tumor cells make important contributions to 'tumor proteolysis'. Many tumors ... More
Live-cell imaging demonstrates extracellular matrix degradation in association with active cathepsin B in caveolae of endothelial cells during tube formation.
Localization of proteases to the surface of endothelial cells and remodeling of the extracellular matrix (ECM) are essential to endothelial cell tube formation and angiogenesis. Here, we partially localized active cathepsin B and its cell surface binding partners, S100A/p11 (p11) of the annexin II heterotetramer (AIIt), to caveolae of human ... More
NMR and bioinformatics discovery of exosites that tune metalloelastase specificity for solubilized elastin and collagen triple helices.
The catalytic domain of metalloelastase (matrix metalloproteinase-12 or MMP-12) is unique among MMPs in exerting high proteolytic activity upon fibrils that resist hydrolysis, especially elastin from lungs afflicted with chronic obstructive pulmonary disease or arteries with aneurysms. How does the MMP-12 catalytic domain achieve this specificity? NMR interface mapping suggests ... More
Combined treatment with serine protease inhibitor aprotinin and matrix metalloproteinase inhibitor Batimastat (BB-94) does not prevent invasion of human esophageal and ovarian carcinoma cells in vivo.
AuthorsDella Porta P, Soeltl R, Krell HW, Collins K, O'Donoghue M, Schmitt M, Krüger A
JournalAnticancer Res
PubMed ID10628317
Many studies have highlighted the role played by matrix metalloproteinases MMP-2 and -9, by serine proteases uPA and plasmin in tumor cell invasion. This study investigates the impact of the MMP-inhibitor Batimastat and/or the serine protease inhibitor Aprotinin on the in vitro proteolytic activity and in vivo invasive behavior the ... More
Imaging proteolysis by living human breast cancer cells.
AuthorsSameni M, Moin K, Sloane BF
JournalNeoplasia
PubMed ID11228542
Malignant progression is accompanied by degradation of extracellular matrix proteins. Here we describe a novel confocal assay in which we can observe proteolysis by living human breast cancer cells (BT20 and BT549) through the use of quenched-fluorescent protein substrates. Degradation thus was imaged, by confocal optical sectioning, as an accumulation ... More
Hyperoxia-induced emphysematous changes in subacute phase of endotoxin-induced lung injury in rats.
AuthorsKohno M, Ishizaka A, Sawafuji M, Koh H, Hirayama Y, Ikeda E, Shiomi T, Ohashi A, Okada Y, Kobayashi K
JournalAm J Physiol Lung Cell Mol Physiol
PubMed ID15003927
We examined the effects of prolonged hyperoxia (75% O(2)) on lung structure and collagen metabolism in the subacute phase of lung injury induced by continuous infusion of endotoxin (LPS) in a rat model. Experimental groups included control, endotoxin alone, endotoxin plus hyperoxia, and hyperoxia alone. Endotoxin-treated rats received a bolus ... More
Twist1-positive epithelial cells retain adhesive and proliferative capacity throughout dissemination.
Authors
JournalBiol Open
PubMed ID27402962
Rational Design of a Humanized Antibody Inhibitor of Cathepsin B.
Authors
JournalBiochemistry
PubMed ID32212642
Kidney Single-Cell Atlas Reveals Myeloid Heterogeneity in Progression and Regression of Kidney Disease.
Authors
JournalJ Am Soc Nephrol
PubMed ID32978267
In-feed resin acids reduce matrix metalloproteinase activity in the ileal mucosa of healthy broilers without inducing major effects on the gut microbiota.
Authors
JournalVet Res
PubMed ID30795808
The regulation of oocyte maturation and ovulation in the closest sister group of vertebrates.
Authors
JournalElife
PubMed ID31573508
CAMKIIγ suppresses an efferocytosis pathway in macrophages and promotes atherosclerotic plaque necrosis.