Microenvironmental contaminations induced by fluorescent lipophilic dyes used for noninvasive in vitro and in vivo cell tracking.
AuthorsLassailly F, Griessinger E, Bonnet D,
JournalBlood
PubMed ID20215639
'Determining how normal and leukemic stem cells behave in vivo, in a dynamic and noninvasive way, remains a major challenge. Most optical tracking technologies rely on the use of fluorescent or bioluminescent reporter genes, which need to be stably expressed in the cells of interest. Because gene transfer in primary ... More
Myeloid cell microsomal prostaglandin E synthase-1 fosters atherogenesis in mice.
AuthorsChen L, Yang G, Monslow J, Todd L, Cormode DP, Tang J, Grant GR, DeLong JH, Tang SY, Lawson JA, Pure E, Fitzgerald GA
Journal
PubMed ID24753592
'Microsomal prostaglandin E synthase-1 (mPGES-1) in myeloid and vascular cells differentially regulates the response to vascular injury, reflecting distinct effects of mPGES-1-derived PGE2 in these cell types on discrete cellular components of the vasculature. The cell selective roles of mPGES-1 in atherogenesis are unknown. Mice lacking mPGES-1 conditionally in myeloid ... More
Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing.
AuthorsKalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A
JournalJ Biomed Opt
PubMed ID17092148
'We develop an optical whole-body imaging technique for monitoring normal and leukemic hematopoietic cell homing in vivo. A recently developed near-infrared (NIR) lipophilic carbocyanine dye 1,1''-dioctadecyl-3,3,3'',3''-tetramethylindotricarbocyanine iodide (DiR) is used to safely and directly label the membranes of human leukemic Pre-B ALL G2 cell lines as well as primary murine ... More
Ligand conjugated low-density lipoprotein nanoparticles for enhanced optical cancer imaging in vivo.
AuthorsChen J, Corbin IR, Li H, Cao W, Glickson JD, Zheng G
JournalJ Am Chem Soc
PubMed ID17428054
Dynamic tracking of stem cells in an acute liver failure model.
AuthorsEzzat T, Dhar DK, Malago M, Olde Damink SW,
JournalWorld J Gastroenterol
PubMed ID22363116
To investigate a dual labeling technique, which would enable real-time monitoring of transplanted embryonic stem cell (ESC) kinetics, as well as long-term tracking. Liver damage was induced in C57/BL6 male mice (n = 40) by acetaminophen (APAP) 300 mg/kg administered intraperitoneally. Green fluorescence protein (GFP) positive C57/BL6 mouse ESCs were ... More
Cyanine-loaded lipid nanoparticles for improved in vivo fluorescence imaging.
AuthorsTexier I, Goutayer M, Da Silva A, Guyon L, Djaker N, Josserand V, Neumann E, Bibette J, Vinet F,
JournalJ Biomed Opt
PubMed ID19895107
Fluorescence is a very promising radioactive-free technique for functional imaging in small animals and, in the future, in humans. However, most commercial near-infrared dyes display poor optical properties, such as low fluorescence quantum yields and short fluorescence lifetimes. In this paper, we explore whether the encapsulation of infrared cyanine dyes ... More
Diolistics: incorporating fluorescent dyes into biological samples using a gene gun.
AuthorsO'Brien JA, Lummis SC,
JournalTrends Biotechnol
PubMed ID17945370
The hand-held gene gun provides a rapid and efficient method of incorporating fluorescent dyes into cells, a technique that is becoming known as diolistics. Transporting fluorescent dyes into cells has, in the past, used predominantly injection or chemical methods. The use of the gene gun, combined with the new generation ... More
Suitability of various membrane lipophilic probes for the detection of trogocytosis by flow cytometry.
AuthorsDaubeuf S, Bordier C, Hudrisier D, Joly E,
JournalCytometry A
PubMed ID19051238
Trogocytosis is a recently discovered phenomenon whereby lymphocytes capture fragments of the plasma membrane from antigen presenting cells (APCs). Using APCs labeled with widely used fluorescent lipophilic probes, we previously described a trogocytosis analysis protocol (TRAP) useful to understand the mechanisms and biological consequences of this process and to identify ... More
Super-resolution fluorescence imaging of organelles in live cells with photoswitchable membrane probes.
AuthorsShim SH, Xia C, Zhong G, Babcock HP, Vaughan JC, Huang B, Wang X, Xu C, Bi GQ, Zhuang X,
JournalProc Natl Acad Sci U S A
PubMed ID22891300
Imaging membranes in live cells with nanometer-scale resolution promises to reveal ultrastructural dynamics of organelles that are essential for cellular functions. In this work, we identified photoswitchable membrane probes and obtained super-resolution fluorescence images of cellular membranes. We demonstrated the photoswitching capabilities of eight commonly used membrane probes, each specific ... More
Applied electric fields accelerate the diffusion rate and increase the diffusion distance of DiI in fixed tissue.
AuthorsSwift MJ, Crago PE, Grill WM
JournalJ Neurosci Methods
PubMed ID15585299
Lipophilic carbocyanine dyes are effective neuronal tracers in fixed tissue. However, their application has been limited by the slow diffusion, short tracing distances, and long durations of incubation in fixed tissue. We used applied dc electric fields, that exerted forces on the cationic dyes, to increase the diffusion velocity and ... More
Use of a night vision intensifier for direct visualization by eye of far-red and near-infrared fluorescence through an optical microscope.
AuthorsSiddiqi MA, Kilduff GM, Gearhart JD
JournalJ Microsc
PubMed ID14629562
We describe the design, construction and testing of a prototype device that allows the direct visualization by eye of far-red and near-infrared (NIR) fluorescence through an optical microscope. The device incorporates a gallium arsenide (GaAs) image intensifier, typically utilized in low-light or 'night vision' applications. The intensifier converts far-red and ... More
DiOLISTIC labeling of neurons from rodent and non-human primate brain slices.
AuthorsSeabold GK, Daunais JB, Rau A, Grant KA, Alvarez VA,
JournalJ Vis Exp
PubMed ID20644510
DiOLISTIC staining uses the gene gun to introduce fluorescent dyes, such as DiI, into neurons of brain slices (Gan et al., 2009; O'Brien and Lummis, 2007; Gan et al., 2000). Here we provide a detailed description of each step required together with exemplary images of good and bad outcomes that ... More
In vivo imaging of specialized bone marrow endothelial microdomains for tumour engraftment.
AuthorsSipkins DA, Wei X, Wu JW, Runnels JM, Côté D, Means TK, Luster AD, Scadden DT, Lin CP
JournalNature
PubMed ID15959517
The organization of cellular niches is known to have a key role in regulating normal stem cell differentiation and regeneration, but relatively little is known about the architecture of microenvironments that support malignant metastasis. Using dynamic in vivo confocal imaging, here we show that murine bone marrow contains unique anatomic ... More
PLEKHA4/kramer Attenuates Dishevelled Ubiquitination to Modulate Wnt and Planar Cell Polarity Signaling.
AuthorsShami Shah A, Batrouni AG, Kim D, Punyala A, Cao W, Han C, Goldberg ML, Smolka MB, Baskin JM
JournalCell Rep
PubMed ID31091453
'Wnt signaling pathways direct key physiological decisions in development. Here, we establish a role for a pleckstrin homology domain-containing protein, PLEKHA4, as a modulator of signaling strength in Wnt-receiving cells. PLEKHA4 oligomerizes into clusters at PI(4,5)P' ... More
Molecular Profiling and Functional Analysis of Macrophage-Derived Tumor Extracellular Vesicles.
AuthorsCianciaruso C, Beltraminelli T, Duval F, Nassiri S, Hamelin R, Mozes A, Gallart-Ayala H, Ceada Torres G, Torchia B, Ries CH, Ivanisevic J, De Palma M
JournalCell Rep
PubMed ID31167148
'Extracellular vesicles (EVs), including exosomes, modulate multiple aspects of cancer biology. Tumor-associated macrophages (TAMs) secrete EVs, but their molecular features and functions are poorly characterized. Here, we report methodology for the enrichment, quantification, and proteomic and lipidomic analysis of EVs released from mouse TAMs (TAM-EVs). Compared to source TAMs, TAM-EVs ... More
Extracellular Vesicles Provide a Means for Tissue Crosstalk during Exercise.
AuthorsWhitham M, Parker BL, Friedrichsen M, Hingst JR, Hjorth M, Hughes WE, Egan CL, Cron L, Watt KI, Kuchel RP, Jayasooriah N, Estevez E, Petzold T, Suter CM, Gregorevic P, Kiens B, Richter EA, James DE, Wojtaszewski JFP, Febbraio MA
JournalCell Metab
PubMed ID29320704
'Exercise stimulates the release of molecules into the circulation, supporting the concept that inter-tissue signaling proteins are important mediators of adaptations to exercise. Recognizing that many circulating proteins are packaged in extracellular vesicles (EVs), we employed quantitative proteomic techniques to characterize the exercise-induced secretion of EV-contained proteins. Following a 1-hr ... More
miR-26a Limits Muscle Wasting and Cardiac Fibrosis through Exosome-Mediated microRNA Transfer in Chronic Kidney Disease.
Authors
JournalTheranostics
PubMed ID31037144
Spatial and Spectral Mapping and Decomposition of Neural Dynamics and Organization of the Mouse Brain with Multispectral Optoacoustic Tomography.
Authors
JournalCell Rep
PubMed ID30840901
Gold nanoparticle based double-labeling of melanoma extracellular vesicles to determine the specificity of uptake by cells and preferential accumulation in small metastatic lung tumors.
Authors
JournalJ Nanobiotechnology
PubMed ID31973696
Exosomes participate in the alteration of muscle homeostasis during lipid-induced insulin resistance in mice.
Authors
JournalDiabetologia
PubMed ID25073444
Exosome-like vesicles released from lipid-induced insulin-resistant muscles modulate gene expression and proliferation of beta recipient cells in mice.
Authors
JournalDiabetologia
PubMed ID26852333
Engineered extracellular vesicle decoy receptor-mediated modulation of the IL6 trans-signalling pathway in muscle.
Authors
JournalBiomaterials
PubMed ID33049461
An off-the-shelf artificial cardiac patch improves cardiac repair after myocardial infarction in rats and pigs.
Authors
JournalSci Transl Med
PubMed ID32269164
Superoxide is an associated signal for apoptosis in axonal injury.
Authors
JournalBrain
PubMed ID20495185
Embryonic progenitor pools generate diversity in fine-scale excitatory cortical subnetworks.
AuthorsEllender TJ, Avery SV, Mahfooz K, Scaber J, von Klemperer A, Nixon SL, Buchan MJ, van Rheede JJ, Gatti A, Waites C, Pavlou HJ, Sims D, Newey SE, Akerman CJ
JournalNat Commun
PubMed ID31745093
The mammalian neocortex is characterized by a variety of neuronal cell types and precise arrangements of synaptic connections, but the processes that generate this diversity are poorly understood. Here we examine how a pool of embryonic progenitor cells consisting of apical intermediate progenitors (aIPs) contribute to diversity within the upper ... More
A Steric Gating Mechanism Dictates the Substrate Specificity of a Rab-GEF.
AuthorsThomas LL, van der Vegt SA, Fromme JC
JournalDev Cell
PubMed ID30528786
Correct localization of Rab GTPases in cells is critical for proper function in membrane trafficking, yet the mechanisms that target Rabs to specific subcellular compartments remain controversial. Guanine nucleotide exchange factors (GEFs) activate and consequently stabilize Rab substrates on membranes, thus implicating GEFs as the primary determinants of Rab localization. ... More