Lipid-dependent bidirectional traffic of apolipoprotein B in polarized enterocytes.
AuthorsMorel E, Demignot S, Chateau D, Chambaz J, Rousset M, Delers F
JournalMol Biol Cell
PubMed ID14565984
'Enterocytes are highly polarized cells that transfer nutrients across the intestinal epithelium from the apical to the basolateral pole. Apolipoprotein B (apoB) is a secretory protein that plays a key role in the transepithelial transport of dietary fatty acids as triacylglycerol. The evaluation of the control of apoB traffic by ... More
Lipid transfer from human epithelial cells to Pneumocystis carinii in vitro.
AuthorsFurlong ST, Koziel H, Bartlett MS, McLaughlin GL, Shaw MM, Jack RM
JournalJ Infect Dis
PubMed ID9041339
'Pneumocystis carinii lipids are similar to host lipids, but it is not known if some of these lipids are acquired from host cells. The ability of P. carinii to incorporate a fluorescent fatty acid analogue (Bodipy-C12) was analyzed, the metabolism of the incorporated lipid by P. carinii was characterized, and ... More
Murine FATP alleviates growth and biochemical deficiencies of yeast fat1Delta strains.
'Saccharomyces cerevisiae is an ideal model eukaryote for studying fatty-acid transport. Yeast are auxotrophic for unsaturated fatty acids when grown under hypoxic conditions or when the fatty-acid synthase inhibitor cerulenin is included in the growth media. The FAT1 gene encodes a protein, Fat1p, which is required for maximal levels of ... More
Liver fatty acid-binding protein colocalizes with peroxisome proliferator activated receptor alpha and enhances ligand distribution to nuclei of living cells.
AuthorsHuang H, Starodub O, McIntosh A, Atshaves BP, Woldegiorgis G, Kier AB, Schroeder F
JournalBiochemistry
PubMed ID14992586
'Although it is hypothesized that long-chain fatty acyl CoAs (LCFA-CoAs) and long-chain fatty acids (LCFAs) regulate transcription in the nucleus, little is known regarding factors that determine the distribution of these ligands to nuclei of living cells. Immunofluorescence colocalization showed that liver fatty acid-binding protein (L-FABP; binds LCFA-CoA as well ... More
Molar quantification by flow cytometry of fatty acid binding to cells using dipyrrometheneboron difluoride derivatives.
AuthorsMacho A, Mishal Z, Uriel J
JournalCytometry
PubMed ID8742176
'Fatty acid analogs of a dipyrrometheneboron difluoride fluorophore (BDY-FA) have recently been developed. Relative to other fluorescent fatty acids, some of these have the advantages of excitation and emission spectra similar to those of fluorescein and of high quantum yield, which permits their use in conventional argon laser cytometry or ... More
A novel fluorescent fatty acid, 5-methyl-BDY-3-dodecanoic acid, is a potential probe in lipid transport studies by incorporating selectively to lipid classes of BHK cells.
AuthorsKasurinen J
JournalBiochem Biophys Res Commun
PubMed ID1417832
'The 5-methyl-BDY-3-dodecanoic acid (B12FA) labelling of BHK cell lipids was analyzed by thin layer and reverse phase column chromatography. Incorporation to phospholipids was selective: over 90% of B12FA label was enriched in phosphatidylcholine. The major molecular species of PC was that containing palmitate as the unlabelled fatty acid. Small amounts ... More
S3-12, Adipophilin, and TIP47 package lipid in adipocytes.
AuthorsWolins NE, Quaynor BK, Skinner JR, Schoenfish MJ, Tzekov A, Bickel PE
JournalJ Biol Chem
PubMed ID15731108
'Animals have evolved mechanisms to maintain circulating nutrient levels when energy demands exceed feeding opportunities. Mammals store most of their energy as triacylglycerol in the perilipin-coated lipid droplets of adipocytes. How newly synthesized triacylglycerol is delivered to perilipin-coated lipid droplets is poorly understood. Perilipin is a member of the evolutionarily ... More
Ganglioside GM2 is substrate for a sialidase in MDCK cells.
AuthorsMarchesini S, Benaglia G, Piccinotti A, Bresciani R, Preti A
JournalFEBS Lett
PubMed ID9645488
GM1 ganglioside carrying a fluorescent fatty acid in substitution of the natural one, has been administered to cultured Madin-Darby canine kidney (MDCK) cells for different pulse times (0.5-24 h), and its metabolic fate was followed. The fluorescent GM2, asialo-GM2, asialo-GM1 and ceramide were the only detectable metabolites. The complete absence ... More
Altered lipid droplet dynamics in hepatocytes lacking triacylglycerol hydrolase expression.
AuthorsWang H, Wei E, Quiroga AD, Sun X, Touret N, Lehner R,
JournalMol Biol Cell
PubMed ID20410140
Lipid droplets (LDs) form from the endoplasmic reticulum (ER) and grow in size by obtaining triacylglycerols (TG). Triacylglycerol hydrolase (TGH), a lipase residing in the ER, is involved in the mobilization of TG stored in LDs for the secretion of very-low-density lipoproteins. In this study, we investigated TGH-mediated changes in ... More
Application of confocal laser scanning microscopy in characterization of chemical enhancers in drug-in-adhesive transdermal patches.
AuthorsQvist MH, Hoeck U, Kreilgaard B, Madsen F, Hovgaard L, Frokjaer S
JournalAAPS PharmSci
PubMed ID12049487
The purpose of this study was to evaluate the application of confocal laser scanning microscopy (CLSM) in the examination of the embedment and the release characteristics of chemical permeation enhancers from transdermal drug delivery systems (TDDSs) of the "drug-in-adhesive" type. The enhancer lauric acid and a lauric acid fluorescing probe ... More
Phosphorothioate oligodeoxyribonucleotides dissociate from cationic lipids before entering the nucleus.
AuthorsMarcusson EG, Bhat B, Manoharan M, Bennett CF, Dean NM
JournalNucleic Acids Res
PubMed ID9518498
Antisense oligonucleotides complementary to specific mRNA sequences are widely used inhibitors of gene expression in vitro and in vivo . In vitro cationic lipids have been demonstrated to increase the pharmacological activity of antisense oligonucleotides by increasing cellular uptake and facilitating nuclear accumulation. We have investigated the intracellular fate of ... More
Separation of phospholipids in microfluidic chip device: application to high-throughput screening assays for lipid-modifying enzymes.
AuthorsLin S, Fischl AS, Bi X, Parce W
JournalAnal Biochem
PubMed ID12633607
Phospholipid molecules such as ceramide and phosphoinositides play crucial roles in signal transduction pathways. Lipid-modifying enzymes including sphingomyelinase and phosphoinositide kinases regulate the generation and degradation of these lipid-signaling molecules and are important therapeutic targets in drug discovery. We now report a sensitive and convenient method to separate these lipids ... More
Uptake and cellular localization of exogenous lipids by Giardia lamblia, a primitive eukaryote.
AuthorsStevens TL, Gibson GR, Adam R, Maier J, Allison-Ennis M, Das S
JournalExp Parasitol
PubMed ID9207743
Giardia lamblia trophozoites are unable to carry out de novo lipid synthesis. It is therefore likely that lipids are acquired from the small intestine of the host, in which the trophozoites are exposed to free and conjugated fatty acids, various sterols, phospholipids, bile acids, and bile-lipid mixed micelles. Here we ... More
A fluorescence-based high-performance liquid chromatographic assay to determine acid ceramidase activity.
AuthorsHe X, Li CM, Park JH, Dagan A, Gatt S, Schuchman EH
JournalAnal Biochem
PubMed ID10527524
Acid ceramidase (N-acylsphingosine amidohydrolase) is the lysosomal enzyme required to hydrolyze the N-acyl linkage between the fatty acid and sphingosine moieties in ceramide. A deficiency of acid ceramidase activity results in the lipid storage disorder, Farber disease. This study reports a new assay method to detect acid ceramidase activity in ... More
Vectorial acylation in Saccharomyces cerevisiae. Fat1p and fatty acyl-CoA synthetase are interacting components of a fatty acid import complex.
AuthorsZou Z, Tong F, Faergeman NJ, Børsting C, Black PN, DiRusso CC
JournalJ Biol Chem
PubMed ID12601005
In Saccharomyces cerevisiae Fat1p and fatty acyl-CoA synthetase (FACS) are hypothesized to couple import and activation of exogenous fatty acids by a process called vectorial acylation. Molecular genetic and biochemical studies were used to define further the functional and physical interactions between these proteins. Multicopy extragenic suppressors were selected in ... More
Interaction of fibroblast growth factor-2 (FGF-2) with free gangliosides: biochemical characterization and biological consequences in endothelial cell cultures.
AuthorsRusnati M, Tanghetti E, Urbinati C, Tulipano G, Marchesini S, Ziche M, Presta M
JournalMol Biol Cell
PubMed ID9950679
Exogenous gangliosides affect the angiogenic activity of fibroblast growth factor-2 (FGF-2), but their mechanism of action has not been elucidated. Here, a possible direct interaction of sialo-glycolipids with FGF-2 has been investigated. Size exclusion chromatography demonstrates that native, but not heat-denatured, 125I-FGF-2 binds to micelles formed by gangliosides GT1b, GD1b, ... More
Lipid and membrane protein transfer from human neutrophils to schistosomes is mediated by ligand binding.
AuthorsRogers RA, Jack RM, Furlong ST
JournalJ Cell Sci
PubMed ID8282756
Attachment of human neutrophils to schistosomula of Schistosoma mansoni involves leukocyte receptors recognizing carbohydrate, complement and/or IgG ligands on the parasite surface. Here, we examined the transfer of a fluorescent fatty acid analog (BOFA) from human neutrophils to schistosomula coated with concanavalin A (Con A), immune serum or nonimmune serum ... More
Uptake and compartmentalization of fluorescent lipid analogs in larval Schistosoma mansoni.
AuthorsFurlong ST, Thibault KS, Morbelli LM, Quinn JJ, Rogers RA
JournalJ Lipid Res
PubMed ID7706934
Recent studies have suggested that host lipids are both a requirement for the human parasite Schistosoma mansoni and may play a role in evasion of host immunity. To study lipid utilization by this organism we have followed the uptake of fluorescent fatty acid and phospholipid analogs in two parasite stages, ... More
Lipid droplet-mitochondria coupling via perilipin 5 augments respiratory capacity but is dispensable for FA oxidation.
Authors
JournalJ Lipid Res
PubMed ID35065923
Temporal analysis of melanogenesis identifies fatty acid metabolism as key skin pigment regulator.
Authors
JournalPLoS Biol
PubMed ID35584084
Single cell transcriptomics identifies a unique adipose lineage cell population that regulates bone marrow environment.
Authors
JournalElife
PubMed ID32286228
Regulation of lipid homeostasis by the TBC protein dTBC1D22 via modulation of the small GTPase Rab40 to facilitate lipophagy.
Authors
JournalCell Rep
PubMed ID34469730
Lipid droplet size directs lipolysis and lipophagy catabolism in hepatocytes.
Authors
JournalJ Cell Biol
PubMed ID31391210
KRAS Controls Pancreatic Cancer Cell Lipid Metabolism and Invasive Potential through the Lipase HSL.
Authors
JournalCancer Res
PubMed ID32816911
Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish.
Authors
JournalElife
PubMed ID34387191
Adipose triglyceride lipase protects renal cell endocytosis in a Drosophila dietary model of chronic kidney disease.
Authors
JournalPLoS Biol
PubMed ID33945525
Hypoxia-induced macropinocytosis represents a metabolic route for liver cancer.
Authors
JournalNat Commun
PubMed ID35177645
Local Mitochondrial ATP Production Regulates Endothelial Fatty Acid Uptake and Transport.