DiA；4-Di-16-ASP（4-（4-（双十六烷基氨基）苯乙烯基）-N-甲基吡啶碘化物）

DiA；4-Di-16-ASP（4-（4-（双十六烷基氨基）苯乙烯基）-<i>N</i>-甲基吡啶碘化物）

引用和文献 (37)

Invitrogen™

DiA；4-Di-16-ASP（4-（4-（双十六烷基氨基）苯乙烯基）-N-甲基吡啶碘化物）

The lipophilic aminostyryl probe DiA inserts into membranes with its two alkyl tails and its fluorophore oriented parallel to the了解更多信息

货号	数量
D3883 又称 D-3883	25 mg

货号 D3883

又称 D-3883

价格（CNY)

4,998.00

添加至购物车

25 mg

价格（CNY)

4,998.00

添加至购物车

The lipophilic aminostyryl probe DiA inserts into membranes with its two alkyl tails and its fluorophore oriented parallel to the phospholipid acyl chain. When this dialkylaminostyryl probe binds to membranes, it exhibits a strong fluorescence enhancement; its fluorescence in water is minimal.

仅供科研使用。不可用于诊断程序。

检测方法荧光

适用于（设备）荧光显微镜

数量25 mg

运输条件室温

产品类型DiA 4-Di-16-ASP

亚细胞定位细胞膜&脂质, Lipids

Unit SizeEach

内容与储存

室温避光储存。

常见问题解答 (FAQ)

我想标记细胞膜，但可选的染料有多种，该如何选择？

如果进行活细胞成像，CellVue和CellMask 质膜染色剂染色最均匀，被细胞内吞的速度最慢。如果想固定和通透细胞（如进行抗体标记），这种产品并不是最佳选择。麦胚凝集素（WGA）偶联物也能标记活细胞，或者甲醛固定的细胞。它们可以在随后的去垢剂（如TritonX-100）通透处理后保存下来。但如果细胞已经进行通透处理，WGA也会标记内部结构。因此，如果细胞已通透，则只能使用靶向质膜蛋白的抗体。亲脂性花青染料（如DiI）会标记活细胞的所有膜结构，而不仅仅是细胞质膜。此网页（https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure/plasma-membrane.html）的内容可帮助您进行选择。

I want to label the plasma membrane of my cells, but there are several dyes to choose from. Which one should I use?

For live-cell imaging, the CellVue and CellMask Plasma Membrane Stains are the most uniform and the slowest to be endocytosed. However, they are not the best choice if you wish to fix and permeabilize your cells, such as for antibody labeling. Wheat germ agglutinin (WGA) conjugates are also able to label live cells, or can label already formaldehyde-fixed cells. They can survive subsequent permeabilization with detergents, such as Triton X-100. If cells are already permeabilized, WGA will label internal structures as well. Thus, only an antibody against a plasma membrane protein can be used if cells are already permeabilized. Lipophilic cyanine dyes, such as DiI, will label all cell membranes in live cells, not just plasma membranes, if left on live cells for extended periods. Following page will help you choose (http://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure/plasma-membrane.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

引用和文献 (37)

引用和文献

Abstract

Carbocyanine postmortem neuronal tracing. Influence of different parameters on tracing distance and combination with immunocytochemistry.

Authors:Lukas JR, Aigner M, Denk M, Heinzl H, Burian M, Mayr R

Journal:J Histochem Cytochem

PubMed ID:9671441

'Carbocyanines (DiI, DiA, DiO) are able to travel along membranes by diffusion and therefore have been used as postmortem neuronal tracers in aldehyde-fixed tissues. Surprisingly, detailed data on the influence of different parameters on tracing distances are still missing. This study was carried out to optimize tracing procedures and to ... More

Interhemispheric asymmetries of the modular structure in human temporal cortex.

Authors:Galuske RA, Schlote W, Bratzke H, Singer W

Journal:Science

PubMed ID:10988077

'Language-relevant processing of auditory signals is lateralized and involves the posterior part of Brodmann area 22. We found that the functional lateralization in this area was accompanied by interhemispheric differences in the organization of the intrinsic microcircuitry. Neuronal tract tracing revealed a modular network of long-range intrinsic connections linking regularly ... More

The location of fluorescence probes with charged groups in model membranes.

Authors:Kachel K, Asuncion-Punzalan E, London E

Journal:Biochim Biophys Acta

PubMed ID:9814853

'The location of commonly used charged fluorescent membrane probes in membranes was determined in order to: (1) investigate the relationship between the structure of hydrophobic molecules and their depth within membranes; and (2) aid interpretation of experiments in which these fluorescent probes are used to examine membrane structure. Membrane depth ... More

Cortical radial glial cells in human fetuses: depth-correlated transformation into astrocytes.

Authors:deAzevedo LC, Fallet C, Moura-Neto V, Daumas-Duport C, Hedin-Pereira C, Lent R

Journal:J Neurobiol

PubMed ID:12717699

'In the human brain, the transformation of radial glial cells (RGC) into astrocytes has been studied only rarely. In this work, we were interested in studying the morphologic aspects underlying this transformation during the fetal/perinatal period, particularly emphasizing the region-specific glial fiber anatomy in the medial cortex. We have used ... More

Vagal efferent and afferent innervation of the rat esophagus as demonstrated by anterograde DiI and DiA tracing: focus on myenteric ganglia.

Authors:Neuhuber WL, Kressel M, Stark A, Berthoud HR

Journal:J Auton Nerv Syst

PubMed ID:9686909

'Anterograde tracing with the carbocyanine tracer DiI and the aminostyrol derivative DiA was used to selectively label fibers from the nucleus ambiguus, dorsal motor nucleus and nodose ganglion, respectively, terminating in the rat esophagus, and to compare them with the innervation of the gastric fundus in the same animals. Ambiguus ... More

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