Carbocyanine postmortem neuronal tracing. Influence of different parameters on tracing distance and combination with immunocytochemistry.
AuthorsLukas JR, Aigner M, Denk M, Heinzl H, Burian M, Mayr R
JournalJ Histochem Cytochem
PubMed ID9671441
'Carbocyanines (DiI, DiA, DiO) are able to travel along membranes by diffusion and therefore have been used as postmortem neuronal tracers in aldehyde-fixed tissues. Surprisingly, detailed data on the influence of different parameters on tracing distances are still missing. This study was carried out to optimize tracing procedures and to ... More
Interhemispheric asymmetries of the modular structure in human temporal cortex.
AuthorsGaluske RA, Schlote W, Bratzke H, Singer W
JournalScience
PubMed ID10988077
'Language-relevant processing of auditory signals is lateralized and involves the posterior part of Brodmann area 22. We found that the functional lateralization in this area was accompanied by interhemispheric differences in the organization of the intrinsic microcircuitry. Neuronal tract tracing revealed a modular network of long-range intrinsic connections linking regularly ... More
The location of fluorescence probes with charged groups in model membranes.
AuthorsKachel K, Asuncion-Punzalan E, London E
JournalBiochim Biophys Acta
PubMed ID9814853
'The location of commonly used charged fluorescent membrane probes in membranes was determined in order to: (1) investigate the relationship between the structure of hydrophobic molecules and their depth within membranes; and (2) aid interpretation of experiments in which these fluorescent probes are used to examine membrane structure. Membrane depth ... More
Cortical radial glial cells in human fetuses: depth-correlated transformation into astrocytes.
'In the human brain, the transformation of radial glial cells (RGC) into astrocytes has been studied only rarely. In this work, we were interested in studying the morphologic aspects underlying this transformation during the fetal/perinatal period, particularly emphasizing the region-specific glial fiber anatomy in the medial cortex. We have used ... More
Vagal efferent and afferent innervation of the rat esophagus as demonstrated by anterograde DiI and DiA tracing: focus on myenteric ganglia.
AuthorsNeuhuber WL, Kressel M, Stark A, Berthoud HR
JournalJ Auton Nerv Syst
PubMed ID9686909
'Anterograde tracing with the carbocyanine tracer DiI and the aminostyrol derivative DiA was used to selectively label fibers from the nucleus ambiguus, dorsal motor nucleus and nodose ganglion, respectively, terminating in the rat esophagus, and to compare them with the innervation of the gastric fundus in the same animals. Ambiguus ... More
The phosphorylation state of the FIGQY tyrosine of neurofascin determines ankyrin-binding activity and patterns of cell segregation.
AuthorsTuvia S, Garver TD, Bennett V
JournalProc Natl Acad Sci U S A
PubMed ID9371782
'Cell-cell recognition and patterning of cell contacts have a critical role in mediating reversible assembly of a variety of transcellular complexes in the nervous system. This study provides evidence for regulation of cell interactions through modulation of ankyrin binding to neurofascin, a member of the L1CAM family of nervous system ... More
Multicolor "DiOlistic" labeling of the nervous system using lipophilic dye combinations.
AuthorsGan WB, Grutzendler J, Wong WT, Wong RO, Lichtman JW
JournalNeuron
PubMed ID10985343
'We describe a technique for rapid labeling of a large number of cells in the nervous system with many different colors. By delivering lipophilic dye-coated particles to neuronal preparations with a "gene gun," individual neurons and glia whose membranes are contacted by the particles are quickly labeled. Using particles that ... More
Localization and retention in vitro of fluorescently labeled aortic baroreceptor terminals on neurons from the nucleus tractus solitarius.
AuthorsMendelowitz D, Yang M, Andresen MC, Kunze DL
JournalBrain Res
PubMed ID1382802
'The anterograde fluorescent tracer DiA was used to visualize baroreceptor fibers and synaptic terminals both in living and fixed tissue. Baroreceptor fibers labeled with DiA terminated as a dense synaptic field in the medial nucleus tractus solitarius (NTS), making synaptic contact on the soma, as well as processes of neurons ... More
Gradual loss of synaptic cartels precedes axon withdrawal at developing neuromuscular junctions.
AuthorsBalice-Gordon RJ, Chua CK, Nelson CC, Lichtman JW
JournalNeuron
PubMed ID8240805
'We have studied the spatial deployment of synapses arising from different axons that converge on the same developing neuromuscular junctions. Labeling the competing synaptic "cartels" with different dyes in mouse muscle showed that, perinatally, each axon adds similar terminal areas, whereas later, areas occupied by the competing cartels diverged by ... More
Dextran amines in neuronal tracing.
AuthorsFritzsch B, Wilm C
JournalTrends Neurosci
PubMed ID1688669
Semaphorin 3A is a chemoattractant for cortical apical dendrites.
AuthorsPolleux F, Morrow T, Ghosh A
JournalNature
PubMed ID10766232
The apical dendrites of pyramidal neurons integrate inputs from various cortical layers and are central to information processing. Here we show that the growth of apical dendrites towards the pial surface is regulated by a diffusible chemoattractant present at high levels near the marginal zone. A major component of this ... More
A beta2 adrenergic receptor signaling complex assembled with the Ca2+ channel Cav1.2.
AuthorsDavare MA, Avdonin V, Hall DD, Peden EM, Burette A, Weinberg RJ, Horne MC, Hoshi T, Hell JW
JournalScience
PubMed ID11441182
The existence of a large number of receptors coupled to heterotrimeric guanine nucleotide binding proteins (G proteins) raises the question of how a particular receptor selectively regulates specific targets. We provide insight into this question by identifying a prototypical macromolecular signaling complex. The beta(2) adrenergic receptor was found to be ... More
Applied electric fields accelerate the diffusion rate and increase the diffusion distance of DiI in fixed tissue.
AuthorsSwift MJ, Crago PE, Grill WM
JournalJ Neurosci Methods
PubMed ID15585299
Lipophilic carbocyanine dyes are effective neuronal tracers in fixed tissue. However, their application has been limited by the slow diffusion, short tracing distances, and long durations of incubation in fixed tissue. We used applied dc electric fields, that exerted forces on the cationic dyes, to increase the diffusion velocity and ... More
Toluidine blue-O is a Nissl bright-field counterstain for lipophilic fluorescent tracers Di-ASP, DiI and DiO.
AuthorsChelvanayagam DK, Beazley LD
JournalJ Neurosci Methods
PubMed ID9128168
The stain toluidine blue-O (tol blue), applied to sections of neural tissue, is shown to be compatible with the vivid fluorescent lipophilic neural tracers 4-(4-dihexadecylaminostyryl)-N-methylpyridinium iodide (Di-ASP), 3,3'-dioctadecyloxacarbocyanine perchlorate (DiO) and 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). As with other Nissl stains, toluidine blue-O fluoresces in the red end of the spectrum but ... More
Recent techniques for tracing pathways in the central nervous system of developing and adult mammals.
AuthorsVercelli A, Repici M, Garbossa D, Grimaldi A
JournalBrain Res Bull
PubMed ID10654576
Over the last 20 years, the choice of neural tracers has increased manyfold, and includes newly introduced anterograde tracers that allow quantitation of single-axon morphologies, and retrograde tracers that can be combined with intracellular fills for the study of dendritic arbors of neurons which have a specific projection pattern. The ... More
Blockage of voltage-gated calcium signaling impairs migration of glial cells in vivo.
AuthorsLohr C, Heil JE, Deitmer JW
JournalGlia
PubMed ID15712206
Migration of glial cells is an essential step in the development of the antennal lobe, the primary olfactory center of insects, to establish well-defined borders between olfactory glomeruli required for odor discrimination. In the present study, we used two-photon microscopy to visualize calcium signaling in developing antennal lobe glial cells ... More
Cell spreading and lamellipodial extension rate is regulated by membrane tension.
AuthorsRaucher D, Sheetz MP
JournalJ Cell Biol
PubMed ID10629223
Cell spreading and motility require the extension of the plasma membrane in association with the assembly of actin. In vitro, extension must overcome resistance from tension within the plasma membrane. We report here that the addition of either amphiphilic compounds or fluorescent lipids that expanded the plasma membrane increased the ... More
Differential control over postganglionic neurons in rat cardiac ganglia by NA and DmnX neurons: anatomical evidence.
AuthorsCheng Z, Zhang H, Guo SZ, Wurster R, Gozal D
JournalAm J Physiol Regul Integr Comp Physiol
PubMed ID14644755
In previous single-labeling experiments, we showed that neurons in the nucleus ambiguous (NA) and the dorsal moto nucleus of the vagus (DmnX) project to intrinsic cardiac ganglia. Neurons in these two motor nuclei differ significantly in the size of their projection fields, axon caliber, and endings in cardiac ganglia. These ... More
Trigeminal ganglion cell processes are spatially ordered prior to the differentiation of the vibrissa pad.
AuthorsErzurumlu RS, Jhaveri S
JournalJ Neurosci
PubMed ID1403092
The rodent trigeminal system is characterized by the punctate organization of its afferents and neurons that replicate the distribution of mystacial vibrissae and sinus hairs on the snout. We have examined the development of topographic equivalence between the sensory periphery on the snout and the brainstem trigeminal nuclei in rats. ... More
Macroscopic consequences of the action of phospholipase C on giant unilamellar liposomes.
Macroscopic consequences of the formation of diacylglycerol by phospholipase C (PC-PLC) in giant 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine (SOPC) unilamellar vesicles (GUVs, diameter 10-100 microm) were studied by phase contrast and fluorescence microscopy. PC-PLC caused a series of fast stepwise shrinkages of fluid SOPC GUVs, continuing until the vesicle disappeared beyond the optical resolution ... More
Early development of functional spatial maps in the zebrafish olfactory bulb.
AuthorsLi J, Mack JA, Souren M, Yaksi E, Higashijima S, Mione M, Fetcho JR, Friedrich RW
JournalJ Neurosci
PubMed ID15958745
In the adult olfactory bulb (OB), particular chemical classes of odorants preferentially activate glomeruli within loosely defined regions, resulting in a coarse and fractured "chemotopic" map. In zebrafish, amino acids and bile acids predominantly stimulate glomeruli in the lateral and medial OB, respectively. We studied the development of these spatial ... More
Glioanatomy assessed by cell-cell interactions and phagocytotic labelling.
AuthorsThanos S, Fischer D, Pavlidis M, Heiduschka P, Bodeutsch N
JournalJ Neurosci Methods
PubMed ID11074094
In the last three decades of research in neuroscience, fluorescent probes have gone from technical tools in the studies of physicochemical reactions, to being versatile tools in developmental neurobiology, neuroanatomy, angiography, neuromorphology, connectivity, cell death and even photodynamic therapy. Fluorescent dyes belong to heterogeneous groups of substances, but the feature ... More
Modular organization of the pontine nuclei: dendritic fields of identified pontine projection neurons in the rat respect the borders of cortical afferent fields.
AuthorsSchwarz C, Thier P
JournalJ Neurosci
PubMed ID7538562
Cortical afferents transferring information destined for the cerebellum terminate in the pontine nuclei (PN) in a divergent and patchy fashion. We investigated whether the form of dendritic fields of pontine projection neurons which are postsynaptic to the cortical afferents are related to this patchy pattern. To this end we used ... More
Early determination of nasal-temporal retinotopic specificity in the eye anlage of the chick embryo.
AuthorsDütting D, Thanos S
JournalDev Biol
PubMed ID7851647
The retinotectal projection of the chick is established between Embryonic Days 3 and 13 (E3 to E13). Fate mappings of the eye anlage by local injections of the fluorescent dyes DiI and DiA revealed that the anteroposterior axis of the optic vesicle corresponds to the nasotemporal axis of the retina. ... More
Organization of pioneer retinal axons within the optic tract of the rhesus monkey.
AuthorsMeissirel C, Chalupa LM
JournalProc Natl Acad Sci U S A
PubMed ID8171011
Retinal ganglion cell axons must make a decision at the embryonic optic chiasm to grow into the appropriate optic tract. To gain insight into the cues that play a role in sorting out the crossed from the uncrossed optic axons, we investigated the sequence of their initial ingrowth in rhesus ... More
Soluble factors from the olfactory bulb attract olfactory Schwann cells.
AuthorsLiu KL, Chuah MI, Lee KK
JournalJ Neurosci
PubMed ID7869124
Olfactory Schwann cells (OSCs) extend processes that ensheathe bundles of olfactory axons as they course from the olfactory epithelium to the olfactory bulb (OB). Results of morphological and immunohistochemical studies have led to speculation that OSCs may be involved in guiding the olfactory axons to their target tissue. In this ... More
Differential fasciculation of follicular nerves for transferring specifically localized cues of the vibrissa rudiments to the central trigeminal sensory system in mice, as exploited with DiI and DiA labeling.
AuthorsYamakado M, Honzawa K, Tsuchiya H
JournalKaibogaku Zasshi
PubMed ID9711026
Vibrissa connections play a decisive role in setting the somatotopic coordinates in the trigeminal sensory system. Although previous studies have examined the development of peripheral patterning, certain questions are still in dispute, for example, the way vibrissa connections are structured, and the relationship between periphery and central organization, and ganglion ... More
Interactions between dorsal root axons and their target motor neurons in developing mammalian spinal cord.
AuthorsSnider WD, Zhang L, Yusoof S, Gorukanti N, Tsering C
JournalJ Neurosci
PubMed ID1326607
We have utilized the lipid-soluble tracers Dil and DiA to investigate interactions between group la dorsal root afferent axons and their target motor neurons in developing rat spinal cord. We show here that la axons project toward motor pools in fascicles that exhibit a considerable degree of spatial order. A ... More
Prenatal development of rat primary afferent fibers: I. Peripheral projections.
AuthorsMirnics K, Koerber HR
JournalJ Comp Neurol
PubMed ID7636033
Development of the peripheral innervation patterns of the L1-S1 lumbosacral ganglia and motor segments in embryonic day 12-17 (E12-17) rat embryos was examined using carbocyanine dyes. Individual dorsal root ganglia (DRGs) and/or isolated ventral horn (VH) segments, or individual peripheral nerves, were isolated in rat embryos fixed at different stages ... More
Prenatal development of rat primary afferent fibers: II. Central projections.
AuthorsMirnics K, Koerber HR
JournalJ Comp Neurol
PubMed ID7636034
These studies were designed to determine the pattern of initial afferent fiber ingrowth into the prenatal spinal gray matter and the establishment of the topographic organization of the presynaptic neuropil in the dorsal horn. A total of 113 lumbar dorsal root ganglia were labeled with carbocyanine fluorescent dye DiI or ... More
Snf2h Drives Chromatin Remodeling to Prime Upper Layer Cortical Neuron Development.
Authors
JournalFront Mol Neurosci
PubMed ID31680852
Thalamocortical axons control the cytoarchitecture of neocortical layers by area-specific supply of VGF.
Authors
JournalElife
PubMed ID35289744
Synthetic high-density lipoprotein nanoparticles for the treatment of Niemann-Pick diseases.
Authors
JournalBMC Med
PubMed ID31711490
Robo1 regulates the development of major axon tracts and interneuron migration in the forebrain.
Authors
JournalDevelopment
PubMed ID16690755
The Ciliopathy Gene Ftm/Rpgrip1l Controls Mouse Forebrain Patterning via Region-Specific Modulation of Hedgehog/Gli Signaling.
Authors
JournalJ Neurosci
PubMed ID30692221
The role of primary cilia in corpus callosum formation is mediated by production of the Gli3 repressor.
Authors
JournalHum Mol Genet
PubMed ID26071364
Continuous focal translation enhances rate of point-scan volumetric microscopy.