Neural transplant staining with DiI and vital imaging by 2-photon laser-scanning microscopy.
AuthorsPotter SM, Pine J, Fraser SE
JournalScanning Microsc Suppl
PubMed ID9601539
We are developing a multielectrode silicon "neuroprobe" for maintaining a long-term, specific, two-way electrical interface with nervous tissue. Our approach involves trapping a neuron (from an embryonic rat hippocampus) in a small well with a stimulation/recording electrode at its base. The well is covered with a grillwork through which the ... More
Cultured postnatal rat septohippocampal neurons change intracellular calcium in response to ethanol and nerve growth factor.
AuthorsWebb B, Suarez SS, Heaton MB, Walker DW
JournalBrain Res
PubMed ID9459553
Ethanol exposure affects cellular mechanisms involved in the regulation of calcium (Ca2+) homeostasis. Neurotrophins, such as nerve growth factor (NGF), stabilize intracellular Ca2+([Ca2+]i) during a variety of neurotoxic insults. In this study, changes in [Ca2+]i during treatment with ethanol and NGF were measured at the cell body of neurons using ... More
Evaluation of a flow cytometric fluorescence quenching assay of phagocytosis of sensitized sheep erythrocytes by polymorphonuclear leukocytes.
AuthorsVan Amersfoort ES, Van Strijp JA
JournalCytometry
PubMed ID7875036
A number of reports have been published describing phagocytosis assays for flow cytometric analysis. In some of these, the fluorescence quenching technique has been used to discriminate between adherent and ingested particles. In this report, we have evaluated the efficacy of a quantitative fluorescence quenching technique with crystal violet and ... More
Migratory pattern of fetal rat brain cells and human glioma cells in the adult rat brain.
AuthorsPedersen PH, Marienhagen K, Mørk S, Bjerkvig R
JournalCancer Res
PubMed ID8221651
The migratory behavior of two human glioma cell lines (D-54MG and GaMG) and fetal rat brain cells grafted into the adult rat brain was studied. To trace the implanted cells, they were stained with the carbocyanine vital dye 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate before injecting them into the white matter above the corpus ... More
Fluorescence study of the macrolide pentaene antibiotic filipin in aqueous solution and in a model system of membranes.
AuthorsCastanho MA, Prieto MJ
JournalEur J Biochem
PubMed ID1628644
The polyene antibiotic filipin (a pentaene) has been studied using photophysical techniques. The polyene self-aggregates in water with a critical micellar concentration of 2 microM. Two approaches were used to evaluate the aggregate dimensions: (a) a lower limit of 10 nm for the aggregate radius was obtained from energy transfer ... More
Flow cytometry as a strategy to study radiation-induced bystander effects in co-culture systems.
AuthorsGerashchenko BI, Howell RW
JournalCytometry A
PubMed ID12820115
BACKGROUND: There is growing interest in the role of bystander effects in the biological response of mammalian cells to ionizing radiation. Qualitative and quantitative assessment of bystander effects requires quick and reliable methodologies. The present work uses a flow cytometric approach to study proliferation of bystander cells co-cultured with irradiated ... More
Immunocytochemical characterisation of the wall of the bovine lateral ventricle.
The cytoarchitecture of the walls of the bovine lateral ventricles was investigated by the use of immunocytochemistry. We defined three types of walls. Type 1 lined regions of white matter and had ciliated cuboidal ependyma, a few subependymal cells and a narrow subjacent glial layer. Type 2 lined the striatum ... More
Uptake of exogenous fluorescent Di-I by intact junctional epithelium of adult rats allows retrograde labeling of trigeminal sensory neurons.
AuthorsSugaya A, Chudler EH, Byers MR
JournalBrain Res
PubMed ID7982069
Junctional epithelium (JE) is the special attachment tissue between gingiva and teeth, and it is well innervated by sensory nerve fibers. We have found that the fluorescent carbocyanine dye, Di-I, can penetrate quickly into intact JE and spread into connective tissue. Di-I containing neurons in trigeminal ganglion were found at ... More
Subzonal organization of olfactory sensory neurons projecting to distinct glomeruli within the mouse olfactory bulb.
AuthorsLevai O, Breer H, Strotmann J
JournalJ Comp Neurol
PubMed ID12619077
'Olfactory sensory neurons located in the nasal neuroepithelium send their axons directly into the olfactory bulb, where they contact the dendrites of second-order neurons in specialized spherical structures called glomeruli; each sensory neuron projects to a single glomerulus. All neurons expressing the same odorant receptor gene are confined to distinct ... More
Semaphorin II can function as a selective inhibitor of specific synaptic arborizations.
'Previous studies showed that grasshopper semaphorin I, a transmembrane semaphorin, functions in vivo to steer a pair of growth cones, prevent defasciculation, and inhibit branching; and that chick collapsin, a secreted semaphorin, can function in vitro to cause growth cone collapse. Semaphorin II, a secreted semaphorin in Drosophila, is transiently ... More
Long aboral projections of Dogiel type II, AH neurons within the myenteric plexus of the guinea pig small intestine.
AuthorsBrookes SJ, Song ZM, Ramsay GA, Costa M
JournalJ Neurosci
PubMed ID7751962
'Enteric AH neurons, with multipolar Dogiel type II morphology, project around the circumference of the intestine to myenteric ganglia, the submucosa and mucosa. Using retrograde labeling in vitro, intracellular recording, dye filling and immunohistochemistry, the projections of these neurons along the intestine were studied. When the retrograde tracer, Dil, was ... More
Antigen-induced translocation of PKC-theta to membrane rafts is required for T cell activation.
AuthorsBi K, Tanaka Y, Coudronniere N, Sugie K, Hong S, van Stipdonk MJ, Altman A
JournalNat Immunol
PubMed ID11376344
'Protein kinase C-theta (PKC-theta) is essential for mature T cell activation; however, the mechanism by which it is recruited to the TCR signaling machinery is unknown. Here we show that T cell stimulation by antibodies or peptide-major histocompatibility complex (MHC) induces translocation of PKC-theta to membrane lipid rafts, which localize ... More
Insect lipoprotein follows a transferrin-like recycling pathway that is mediated by the insect LDL receptor homologue.
AuthorsVan Hoof D, Rodenburg KW, Van der Horst DJ
JournalJ Cell Sci
PubMed ID12356906
'The lipoprotein of insects, high-density lipophorin (HDLp), is homologous to that of mammalian low-density lipoprotein (LDL) with respect to its apolipoprotein structure. Moreover, an endocytic receptor for HDLp has been identified (insect lipophorin receptor, iLR) that is homologus to the LDL receptor. We transfected LDL-receptor-expressing CHO cells with iLR cDNA ... More
Redefining the role of metallothionein within the injured brain: extracellular metallothioneins play an important role in the astrocyte-neuron response to injury.
AuthorsChung RS, Penkowa M, Dittmann J, King CE, Bartlett C, Asmussen JW, Hidalgo J, Carrasco J, Leung YK, Walker AK, Fung SJ, Dunlop SA, Fitzgerald M, Beazley LD, Chuah MI, Vickers JC, West AK,
JournalJ Biol Chem
PubMed ID18334482
'A number of intracellular proteins that are protective after brain injury are classically thought to exert their effect within the expressing cell. The astrocytic metallothioneins (MT) are one example and are thought to act via intracellular free radical scavenging and heavy metal regulation, and in particular zinc. Indeed, we have ... More
Cocaine-induced dendritic spine formation in D1 and D2 dopamine receptor-containing medium spiny neurons in nucleus accumbens.
AuthorsLee KW, Kim Y, Kim AM, Helmin K, Nairn AC, Greengard P
JournalProc Natl Acad Sci U S A
PubMed ID16492766
'Psychostimulant-induced alteration of dendritic spines on dopaminoceptive neurons in nucleus accumbens (NAcc) has been hypothesized as an adaptive neuronal response that is linked to long-lasting addictive behaviors. NAcc is largely composed of two distinct subpopulations of medium-sized spiny neurons expressing high levels of either dopamine D1 or D2 receptors. In ... More
High density lipoprotein (HDL) particle uptake mediated by scavenger receptor class B type 1 results in selective sorting of HDL cholesterol from protein and polarized cholesterol secretion.
AuthorsSilver DL, Wang N, Xiao X, Tall AR
JournalJ Biol Chem
PubMed ID11301333
'High density lipoprotein (HDL) mediates reverse transport of cholesterol from atheroma foam cells to the liver, but the mechanisms of hepatic uptake and trafficking of HDL particles are poorly understood. In contrast to its accepted role as a cell surface receptor, scavenger receptor class B type 1 (SR-BI) is shown ... More
Slit proteins prevent midline crossing and determine the dorsoventral position of major axonal pathways in the mammalian forebrain.
AuthorsBagri A, Marín O, Plump AS, Mak J, Pleasure SJ, Rubenstein JL, Tessier-Lavigne M
JournalNeuron
PubMed ID11804571
'We report that Slit proteins, a family of secreted chemorepellents, are crucial for the proper development of several major forebrain tracts. Mice deficient in Slit2 and, even more so, mice deficient in both Slit1 and Slit2 show significant axon guidance errors in a variety of pathways, including corticofugal, callosal, and ... More
Reduction of connexin43 expression and dye-coupling during neuronal differentiation of human NTera2/clone D1 cells.
AuthorsBani-Yaghoub M, Bechberger JF, Naus CC
JournalJ Neurosci Res
PubMed ID9211986
'Gap junctions are plasma membrane specializations that allow direct communication among adjoining cells. We used a human pluripotential teratocarcinoma cell line, NTera-2/clone D1 (NT2/D1), as a model to study gap junctions in CNS neurons and their neuronal precursors. These cells were differentiated following retinoic acid (RA) treatment for 4 weeks ... More
Retinal ganglion cell genesis requires lakritz, a Zebrafish atonal Homolog.
AuthorsKay JN, Finger-Baier KC, Roeser T, Staub W, Baier H
JournalNeuron
PubMed ID11430806
'Mutation of the zebrafish lakritz (lak) locus completely eliminates the earliest-born retinal cells, the ganglion cells (RGCs). Instead, excess amacrine, bipolar, and Müller glial cells are generated in the mutant. The extra amacrines are found at ectopic locations in the ganglion cell layer. Cone photoreceptors appear unaffected by the mutation. ... More
Lipid rafts in the maintenance of synapses, dendritic spines, and surface AMPA receptor stability.
AuthorsHering H, Lin CC, Sheng M
JournalJ Neurosci
PubMed ID12716933
'Cholesterol/sphingolipid microdomains (lipid rafts) in the membrane are involved in protein trafficking, formation of signaling complexes, and regulation of actin cytoskeleton. Here, we show that lipid rafts exist abundantly in dendrites of cultured hippocampal neurons, in which they are associated with several postsynaptic proteins including surface AMPA receptors. Depletion of ... More
Isolation of a somatic cell mutant resistant to the induction of apoptosis by oxidized low density lipoprotein.
AuthorsRusiñol AE, Yang L, Thewke D, Panini SR, Kramer MF, Sinensky MS
JournalJ Biol Chem
PubMed ID10702300
'Oxidized low density lipoprotein (oxLDL) induces apoptosis in macrophages, smooth muscle cells, and endothelial cells. To elucidate the molecular mechanism of oxLDL-induced cytotoxicity and determine its tissue specificity, we have used Chinese hamster ovary (CHO)-K1 cells expressing human CD36 (CHO/CD36). Expression of CD36 rendered these cells susceptible to killing by ... More
Synaptogenesis in hippocampal cultures: evidence indicating that axons and dendrites become competent to form synapses at different stages of neuronal development.
AuthorsFletcher TL, De Camilli P, Banker G
JournalJ Neurosci
PubMed ID7965070
'Hippocampal neurons in culture develop extensive axonal and dendritic arbors and form numerous synapses. Presynaptic specializations occur at sites of contact between axons and somata or dendrites but they do not appear until day 3 in culture, even though numerous contacts between cells develop within the first 24 hr (Fletcher ... More
Phosphatidylinositol 4,5-bisphosphate and Arf6-regulated membrane traffic.
'ADP-ribosylation factor (Arf) 6 regulates the movement of membrane between the plasma membrane (PM) and a nonclathrin-derived endosomal compartment and activates phosphatidylinositol 4-phosphate 5-kinase (PIP 5-kinase), an enzyme that generates phosphatidylinositol 4,5-bisphosphate (PIP2). Here, we show that PIP2 visualized by expressing a fusion protein of the pleckstrin homology domain from ... More
Neuroanatomical evidence for segregation of nerve fibers conveying light touch and pain sensation in Eimer's organ of the mole.
AuthorsMarasco PD, Tsuruda PR, Bautista DM, Julius D, Catania KC
JournalProc Natl Acad Sci U S A
PubMed ID16751268
'Talpid moles are small insectivores that live in dark underground tunnels. They depend heavily on touch to navigate and find food. Most species have an array of complex epidermal sensory structures called Eimer''s organs that cover the tip of the nose. In this study, the anatomy of Eimer''s organ was ... More
The use of the lipophilic fluorochrome CM-DiI for tracking the migration of lymphocytes.
AuthorsAndrade W, Seabrook TJ, Johnston MG, Hay JB
JournalJ Immunol Methods
PubMed ID8765171
'In this study we examined the new cell dye CM-DiI for tracking the migration of lymphocytes from blood to lymph. This lipophilic marker intercalates in the plasma membrane like the PKH dyes and older DiI derivatives. The stability and intensity of staining achieved with these dyes is better than most ... More
Tissue engineering for the regeneration of the mastoid air cells: a preliminary in vitro study.
AuthorsMagrufov A, Kanemaru S, Nakamura T, Omori K, Yamashita M, Shimizu Y, Ito J
JournalActa Otolaryngol Suppl
PubMed ID15078084
'Mastoid is a pneumatic bone, composed of small interconnecting chambers covered by a mono-layer of mucosa with an abundant blood supply. One of its main functions is gas exchange according to the concentration/pressure gradient. The final goal of our research project is to regenerate mastoid air cells and their unique ... More
Interactions between fetal rat brain cells and mature brain tissue in vivo and in vitro.
AuthorsMarienhagen K, Pedersen PH, Terzis AJ, Laerum OD, Arnold H, Bjerkvig R
JournalNeuropathol Appl Neurobiol
PubMed ID8072644
'Fetal as well as mature neural cells were homografted into the right cerebral hemisphere of adult BD-IX rats. The animals were sacrificed 7 d after implantation, and the localization of implanted cells was visualized by fluorescence and light microscopy. The cell implants were prestained with the fluorescent vital dye 1,1'-Dioctadecyl-3,3,3'3'-tetramethylindocarbocyanine ... More
Beta-very low density lipoprotein is sequestered in surface-connected tubules in mouse peritoneal macrophages.
AuthorsMyers JN, Tabas I, Jones NL, Maxfield FR
JournalJ Cell Biol
PubMed ID8253839
'beta-very low density lipoprotein (VLDL) is a large lipoprotein with multiple apoprotein E (apoE) molecules that bind to the LDL receptors on mouse macrophages. Even though they bind to the same receptor, the endocytic processing of beta-VLDL differs from low density lipoprotein (LDL). LDL is rapidly delivered to perinuclear lysosomes ... More
Dendritic distribution of two populations of ganglion cells and the retinopetal fibers in the retina of the silver lamprey (Ichthyomyzon unicuspis).
AuthorsFritzsch B, Collin SP
JournalVis Neurosci
PubMed ID2278933
'The distribution of ganglion cells in the retina of the silver lamprey, Ichthyomyzon unicuspis, was revealed by retrograde labeling from the optic nerve with horseradish peroxidase (HRP) and fluorescent-labeled dextrans in live animals and with the fluorescent dye DiI in aldehyde-fixed tissue. The majority of ganglion cells (74%) termed the ... More
Regulation of C-cadherin function during activin induced morphogenesis of Xenopus animal caps.
AuthorsBrieher WM, Gumbiner BM
JournalJ Cell Biol
PubMed ID8034750
'Treatment of Xenopus animal pole tissue with activin results in the induction of mesodermal cell types and a dramatic elongation of the tissue. The morphogenetic movements involved in the elongation appear similar to those in normal gastrulation, which is driven by cell rearrangement and cell intercalations. We have used this ... More
Intracellular transport of high-density lipoprotein 3 in intestinal epithelial cells (Caco-2) is tubulin associated.
AuthorsRogler G, Aschenbrenner E, Gross V, Stange EF, Schölmerich J
JournalDigestion
PubMed ID10671774
'BACKGROUND: A retroendocytotic pathway for high-density lipoprotein 3 (HDL(3)) in cultured intestinal epithelial cell lines has been described. In small intestinal crypt cells and Caco-2, HDL(3) is internalized, transported to lipid droplets and, after solubilization of these lipid droplets, resecreted. In the present study we examined the mechanisms of intracellular ... More
Inaccuracies in initial growth and arborization of chick retinotectal axons followed by course corrections and axon remodeling to develop topographic order.
AuthorsNakamura H, O'Leary DD
JournalJ Neurosci
PubMed ID2585055
'The retinotectal projection is organized in a precise retinotopic manner. We find, though, that during development the growth and arborization of temporal retinal axons within the optic tectum of chick embryos is initially imprecise. Axonal targeting errors occur along the rostral-caudal and medial-lateral tectal axes, and arbors are formed at ... More
Identification of motor neurons to the circular muscle of the guinea pig gastric corpus.
AuthorsBrookes SJ, Hennig G, Schemann M
JournalJ Comp Neurol
PubMed ID9658288
'The projections of enteric neurons to the circular muscle of the guinea pig gastric corpus were investigated systematically by using the retrogradely transported fluorescent carbocyanine dye 1,1''-didodecyl-3,3,3'',3''-tetramethyl indocarbocyanine perchlorate (DiI), applied to the muscle layer or myenteric plexus in vitro. DiI-labeled motor neuron cell bodies were located up to 6.3 ... More
Organization of corticocortical connections in human visual cortex.
AuthorsBurkhalter A, Bernardo KL
JournalProc Natl Acad Sci U S A
PubMed ID2464827
'Clinical and psychophysical observations indicate that the visual cortex is critical for the perception of color, form, depth, and movement. Little, however, is known about the cortical circuitry that underlies these functions in humans. In an attempt to learn more about these connections, we have traced projections of primary (V1) ... More
An epithelial cell destined for apoptosis signals its neighbors to extrude it by an actin- and myosin-dependent mechanism.
AuthorsRosenblatt J, Raff MC, Cramer LP
JournalCurr Biol
PubMed ID11728307
'BACKGROUND: Simple epithelia encase developing embryos and organs. Although these epithelia consist of only one or two layers of cells, they must provide tight barriers for the tissues that they envelop. Apoptosis occurring within these simple epithelia could compromise this barrier. How, then, does an epithelium remove apoptotic cells without ... More
Particle size of liposomes influences dermal delivery of substances into skin.
AuthorsVerma DD, Verma S, Blume G, Fahr A
JournalInt J Pharm
PubMed ID12753761
'In the present study, the influence of vesicle size on the penetration of two fluorescently labeled substances into the human skin was investigated. For the measurements either a hydrophilic fluorescent compound [carboxyfluorescein (CF)] or a lipophilic one [1,1''-dioctadecyl-3,3,3'',3''-tertramethylindocarbo-cyanine perchlorate (DiI)] were encapsulated into vesicles. Liposomal formulations were prepared by extruding ... More
The multicollisional, obstructed, long-range diffusional nature of mitochondrial electron transport.
AuthorsChazotte B, Hackenbrock CR
JournalJ Biol Chem
PubMed ID3170548
'Data are presented which reveal that ubiquinone (Q)-mediated electron transport is a multicollisional, obstructed, long-range diffusion process, where factors that affect the rate of lateral diffusion also affect the rate of electron transport. Based on fluorescence recovery after photobleaching measurements, it was concluded that Q-mediated electron transport occurs by the ... More
Structural changes of the endoplasmic reticulum of sea urchin eggs during fertilization.
AuthorsJaffe LA, Terasaki M
JournalDev Biol
PubMed ID8462752
'The endoplasmic reticulum (ER) of the sea urchin egg includes a system of cisternae, a cortical tubular network, and the nuclear envelope. In previous work (Terasaki and Jaffe, 1991, J. Cell. Biol. 114, 929-940) we showed that the long chain fluorescent lipophilic dye DiI spread from an intracellularly injected oil ... More
Changes in the spectral properties of a plasma membrane lipid analog during the first seconds of endocytosis in living cells.
AuthorsChen CS, Martin OC, Pagano RE
JournalBiophys J
PubMed ID8994591
'N-[5-(5, 7-dimethyl Bodipy)-1-pentanoyl]-D-erythro-sphingosylphosphorylcholine (C5-DMB-SM), a fluorescent analog of sphingomyelin, has been used in a study of the formation of very early endosomes in human skin fibroblasts. This lipid exhibits a shift in its fluorescence emission maximum from green (approximately 515 nm) to red (approximately 620 nm) wavelengths with increasing concentrations ... More
Anatomical and physiological localization of prelabeled grafts in rat hippocampus.
AuthorsPyapali GK, Turner DA, Madison RD
JournalExp Neurol
PubMed ID1577121
'Dissociated rat fetal hippocampal cells were grafted into normal adult rats. The fetal cells were incubated with one of a number of fluorescent compounds at the time of the dissociation to facilitate identification of the individual grafted cells. The fluorescent labels which were analyzed for this purpose included rhodamine latex ... More
Relationship between spatially restricted Krox-20 gene expression in branchial neural crest and segmentation in the chick embryo hindbrain.
AuthorsNieto MA, Sechrist J, Wilkinson DG, Bronner-Fraser M
JournalEMBO J
PubMed ID7537662
'Previous studies have suggested that the rostrocaudal patterning of branchial arches in the vertebrate embryo derives from a coordinate segmental specification of gene expression in rhombomeres (r) and neural crest. However, expression of the Krox-20 gene is restricted to neural crest cells migrating to the third branchial arch, apparently from ... More
Backward cortical projections to primary somatosensory cortex in rats extend long horizontal axons in layer I.
AuthorsCauller LJ, Clancy B, Connors BW
JournalJ Comp Neurol
PubMed ID9453672
'We have studied the origin and extent of axons within layer I of the primary somatosensory cortex (SI) of rats by using retrograde and anterograde tracers with an emphasis on reciprocal connections to layer I of SI from ipsilateral cortical areas that are the target of SI projections. Small crystals ... More
Target pioneering and early morphology of the murine chorda tympani.
AuthorsScott L, Atkinson ME
JournalJ Anat
PubMed ID9568564
'Many studies demonstrate that differentiation of certain sensory receptors during development is induced by their nerve supply. Thus the navigational accuracy of pioneering fibres to their targets is crucial to this process. The special gustatory elements of the facial and glossopharyngeal nerves are used extensively as model systems in this ... More
Selective intraportal transplantation of DiI-marked isolated rat hepatocytes.
AuthorsMarkus PM, Koenig S, Krause P, Becker H
JournalCell Transplant
PubMed ID9331496
'Transplantation of isolated hepatocytes is a promising alternative to orthotopic liver transplantation in experimental animal models with acute hepatic failure and hereditary enzyme defects. Conventional light microscopy identification of hepatocytes within recipient livers has been limited due to the inability to distinguish between donor and recipient liver cells. In this ... More
Developmental changes in the distribution of the endoplasmic reticulum and inositol 1,4,5-trisphosphate receptors and the spatial pattern of Ca2+ release during maturation of hamster oocytes.
AuthorsShiraishi K, Okada A, Shirakawa H, Nakanishi S, Mikoshiba K, Miyazaki S
JournalDev Biol
PubMed ID7649386
'During maturation of hamster oocytes, the distribution of the endoplasmic reticulum (ER) and inositol 1,4,5-trisphosphate receptors (InsP3Rs) was found to change dramatically, as observed using confocal microscopy with DiI and electron microscopy for the ER and immunohistochemistry for InsP3Rs. In immature oocytes at the germinal vesicle (GV) stage, ER and ... More
Mechanisms of adoptive immunotherapy: improved methods for in vivo tracking of tumor-infiltrating lymphocytes and lymphokine-activated killer cells.
AuthorsWallace PK, Palmer LD, Perry-Lalley D, Bolton ES, Alexander RB, Horan PK, Yang JC, Muirhead KA
JournalCancer Res
PubMed ID8485722
'Adoptive immunotherapy with tumor-infiltrating lymphocytes (TIL) and lymphokine-activated killer cells has been demonstrated to mediate regression of tumors in murine models and in selected patients with advanced cancer. Improved methods for monitoring immune cell traffic, particularly to sites of tumor, are needed to elucidate mechanisms of antitumor activity and optimize ... More
Differential cellular accumulation/retention of apolipoprotein E mediated by cell surface heparan sulfate proteoglycans. Apolipoproteins E3 and E2 greater than e4.
AuthorsJi ZS, Pitas RE, Mahley RW
JournalJ Biol Chem
PubMed ID9593678
'Isoform-specific effects of apolipoprotein E (apoE) on neurite outgrowth and the cytoskeleton are associated with higher intracellular levels of apoE3 than apoE4 in cultured neurons. The current studies, designed to determine the mechanism for the differential intracellular accumulation or retention of apoE, demonstrate that apoE3- and apoE4-containing beta-very low density ... More
Deviation of the blastocyst axis from the first cleavage plane does not affect the quality of mouse postimplantation development.
AuthorsAlarcón VB, Marikawa Y
JournalBiol Reprod
PubMed ID12773417
'Several researchers have suggested recently that the embryonic-abembryonic (Em-Ab) axis of the mouse blastocyst is orthogonal to the first cleavage plane of the two-cell embryo. To determine the universality of this relationship, we used embryos of two different genotypes, F1 (C57BL/6 x DBA/2) and CD-1. The position of the first ... More
Carbocyanine postmortem neuronal tracing. Influence of different parameters on tracing distance and combination with immunocytochemistry.
AuthorsLukas JR, Aigner M, Denk M, Heinzl H, Burian M, Mayr R
JournalJ Histochem Cytochem
PubMed ID9671441
'Carbocyanines (DiI, DiA, DiO) are able to travel along membranes by diffusion and therefore have been used as postmortem neuronal tracers in aldehyde-fixed tissues. Surprisingly, detailed data on the influence of different parameters on tracing distances are still missing. This study was carried out to optimize tracing procedures and to ... More
Molecular counting of low-density lipoprotein particles as individuals and small clusters on cell surfaces.
AuthorsGross D, Webb WW
JournalBiophys J
PubMed ID3719072
'We employ the intensely fluorescent analogue diI-LDL (Barak, L. S., and W. W. Webb, 1981, J. Cell Biol. 90:595-604) as a counting marker to determine the numbers of LDL-receptor complexes that are contained in clusters on the surfaces of human fibroblasts and human epidermoid carcinoma cells. The application of quantitative ... More
Altered segmental identity and abnormal migration of motor neurons in mice lacking Hoxb-1.
AuthorsStuder M, Lumsden A, Ariza-McNaughton L, Bradley A, Krumlauf R
JournalNature
PubMed ID8967950
'Segmentation of the vertebrate hindbrain into rhombomeres is important for the anterior-posterior arrangement of cranial motor nuclei and efferent nerves. Underlying this reiterated organization, Hox genes display segmentally restricted domains of expression, such as expression of Hoxb-1 (refs 5, 6) in rhombomere 4 (r4). Here we report that absence of ... More
A flow cytometric method to assess antigen-specific proliferative responses of different subpopulations of fresh and cryopreserved human peripheral blood mononuclear cells.
AuthorsAllsopp CE, Nicholls SJ, Langhorne J
JournalJ Immunol Methods
PubMed ID9692869
'We have used PKH26 dye, which is incorporated stably into the membrane of cells, to determine, using flow cytometry, lymphocyte proliferative responses to the antigen tetanus toxoid in fresh and cryopreserved samples. Measuring cell proliferation with this dye has advantages over either 3H-thymidine or Bromodeoxyuridine (BrdU). Whereas the existing methods ... More
Tumor cell invasion of model 3-dimensional matrices: demonstration of migratory pathways, collagen disruption, and intercellular cooperation.
'We report a novel 3-dimensional model for visualizing tumor cell migration across a nylon mesh-supported gelatin matrix. To visualize migration across these model barriers, cell proteolytic activity of the pericellular matrix was detected using Bodipy-BSA (fluorescent upon proteolysis) and DQ collagen (fluorescent upon collagenase activity). For 3-dimensional image reconstruction, multiple ... More
Direct contact with stroma inhibits proliferation of human long-term culture initiating cells.
AuthorsVerfaillie CM, Catanzaro P
JournalLeukemia
PubMed ID8642869
'Significantly less long-term culture initiating cells (LTC-IC) are recovered from cultures in which progenitors are cultured in contact with stroma (stroma-contact) than when cultured separated from stroma by a transwell (stroma-noncontact). This suggests that direct contact with stroma inhibits either proliferation or survival of LTC-IC. Using the membrane intercallating fluorochrome, ... More
Lateral diffusion and retrograde movements of individual cell surface components on single motile cells observed with Nanovid microscopy.
Authorsde Brabander M, Nuydens R, Ishihara A, Holifield B, Jacobson K, Geerts H
JournalJ Cell Biol
PubMed ID1670778
'A recently introduced extension of video-enhanced light microscopy, called Nanovid microscopy, documents the dynamic reorganization of individual cell surface components on living cells. 40-microns colloidal gold probes coupled to different types of poly-L-lysine label negative cell surface components of PTK2 cells. Evidence is provided that they bind to negative sialic ... More
Delivery of ligands from sorting endosomes to late endosomes occurs by maturation of sorting endosomes.
AuthorsDunn KW, Maxfield FR
JournalJ Cell Biol
PubMed ID1560027
'After endocytosis, lysosomally targeted ligands pass through a series of endosomal compartments. The endocytic apparatus that accomplishes this passage may be considered to take one of two forms: (a) a system in which lysosomally targeted ligands pass through preexisting, long-lived early sorting endosomes and are then selectively transported to long-lived ... More
Dendritic spines lost during glutamate receptor activation reemerge at original sites of synaptic contact.
AuthorsHasbani MJ, Schlief ML, Fisher DA, Goldberg MP
JournalJ Neurosci
PubMed ID11264313
'During cerebral ischemia, neurons undergo rapid alterations in dendritic structure consisting of focal swelling and spine loss. We used time-lapse microscopy to determine the fate of dendritic spines that disappeared after brief, sublethal hypoxic or excitotoxic exposures. Dendrite and spine morphology were assessed in cultured cortical neurons expressing yellow fluorescent ... More
Programmed cell death of an identified motoneuron in vitro: temporal requirements for steroid exposure and protein synthesis.
AuthorsHoffman KL, Weeks JC
JournalJ Neurobiol
PubMed ID9622013
'Ecdysteroid hormones trigger the programmed cell death (PCD) of a segmental subset of accessory planta retractor (APR) motoneurons at pupation in the moth, Manduca sexta. APRs from abdominal segment four [APR (4)s] survive through the pupal stage, whereas homologous APR(6)s die 24-48 h after pupal ecdysis (PE) (the shedding of ... More
CD95 (Fas)-based, superantigen-dependent, CD4+ T cell-mediated down-regulation of human in vitro immunoglobulin responses.
AuthorsStohl W, Elliott JE, Lynch DH, Kiener PA
JournalJ Immunol
PubMed ID9605118
'Naturally occurring microbial superantigens (SAg) have been implicated in several human idiopathic disorders, and a compelling argument for the role of SAg in autoantibody-associated disorders, such as systemic lupus erythematosus, has been proposed. To test the effects of SAg on human in vitro Ig responses, CD4+ T cell + B ... More
Ganglion cell neurogenesis, migration and early differentiation in the chick retina.
AuthorsSnow RL, Robson JA
JournalNeuroscience
PubMed ID8152546
'Neurogenesis, migration and maturation of ganglion cells in the posterior pole of chick retina have been studied using embryonic incorporation of [3H]thymidine, immunocytochemistry and retrograde labeling. Unlike previous studies, we have examined the neurogenesis of independently identified ganglion cells that have survived the period of naturally occurring cell death (embryonic ... More
Transcellular labeling of taste bud cells by carbocyanine dye (DiI) applied to peripheral nerves in the barbels of the catfish, Ictalurus punctatus.
AuthorsFinger TE, Böttger B
JournalJ Comp Neurol
PubMed ID2081819
'In order to study the pattern of innervation of taste buds and the surrounding epithelium, the carbocyanine dye diI was applied to the nerve stump in isolated, paraformaldehyde-fixed barbels obtained from channel catfish, Ictalurus punctatus. After a diffusion period of 7-41 days, the barbels were sectioned on a vibratome and ... More
Diffusion, patching, and capping of stearoylated dextrans on 3T3 cell plasma membranes.
AuthorsWolf DE, Henkart P, Webb WW
JournalBiochemistry
PubMed ID6157402
'Fluorescence-labeled trinitrophenylated stearoylated dextrans have been used as controllable analogues of cell membrane proteins on model membranes and on a variety of natural cell membranes. This paper reports their behavior on 3T3 mouse fibroblast plasma membranes. Spatial distribution on the membrane was studied by fluorescence microscopy, and molecular mobility was ... More
Membrane changes in lipopolysaccharide-stimulated murine B lymphocytes associated with cell activation.
AuthorsPrinten JA, Woodard SL, Herman JR, Roess DA, Barisas BG
JournalBiochim Biophys Acta
PubMed ID8499473
'The lateral diffusion of the fluorescent lipid analog 3,3''-dioctadecylindocarbocyanine iodide (DiI) was measured in the membranes of murine B lymphocytes treated with the B cell mitogen lipopolysaccharide (LPS). The mobility of DiI, as measured by fluorescence photobleaching recovery (FPR) techniques, was temperature-dependent with a value of 6.1.10(-9) cm2 s-1 at ... More
Experimental study of the connections of the gustatory system in the rainbow trout, Oncorhynchus mykiss.
AuthorsFolgueira M, Anadón R, Yáñez J
JournalJ Comp Neurol
PubMed ID12975819
'Salmonids are a group of teleosts with a nonspecialized gustatory system. With the aim of describing the gustatory connections in a member of this group, we carried out tract-tracing experiments using the lipophilic carbocyanine dye 1,1''-dioctadecyl 3,3,3'',3''-tetramethylindocarbocyanine perchlorate (DiI) in fixed brains of the rainbow trout (Oncorhynchus mykiss). The neural ... More
The mapping of the visual field onto the dorso-lateral tectum of the pigeon (Columba livia) and its relations with retinal specializations.
AuthorsLetelier JC, Marin G, Sentis E, Tenreiro A, Fredes F, Mpodozis J
JournalJ Neurosci Methods
PubMed ID14706713
'Most of the physiological studies of the pigeon retino-tectal visual pathway have investigated the accessible tectum, a small dorso-lateral tectal section that can be easily accessed by a simple craniotomy. However, at present we lack a detailed study of the topographical arrangement between the visual field, the retina and the ... More
Migration of Langerhans cells in an in vitro organ culture system: IL-6 and TNF-alpha are partially responsible for migration into the epidermis.
AuthorsSaitoh A, Yasaka N, Osada A, Nakamura K, Furue M, Tamaki K
JournalJ Dermatol Sci
PubMed ID10215188
'Although it is well established that epidermal Langerhans cells (LC) originate from bone marrow, little is known about the mechanism of this migration into the epidermis from bone marrow. In order to clarify the mechanism of this migration, we constructed an in vitro model. LC were depleted by daily topical ... More
Direct visualization of lipid deposition and reverse lipid transport in a perfused artery : roles of VLDL and HDL.
AuthorsRutledge JC, Mullick AE, Gardner G, Goldberg IJ
JournalCirc Res
PubMed ID10764410
'The major goal of this study was to determine the interactions of VLDL surface and core lipids with the artery wall. We first demonstrated in vitro that surface lipid in VLDL could be traced using the phospholipid-like fluorescent probe 1,1''-dioctadecyl-3,3, 3'',3''-tetramethyl-indocarbocyanine (DiI). The core of VLDL particles was traced by ... More
Induction of a second neural axis by the mouse node.
AuthorsBeddington RS
JournalDevelopment
PubMed ID8162859
'The anterior aspect of the mouse primitive streak resembles the organizer of Xenopus and chick in terms of its developmental fate, ability to alter pattern in the chick limb bud and with respect to the repertoire of genes that its constituent cells express. However, until now there has been no ... More
Limited topographic specificity in the targeting and branching of mammalian retinal axons.
AuthorsSimon DK, O'Leary DD
JournalDev Biol
PubMed ID1688537
'We have studied in rats the topographic targeting of retinocollicular axons anterogradely labeled by focal retinal injections of the axon tracer DiI. We find that developing retinal axons widely mistarget along both the medial-lateral and the rostral-caudal axes of the superior colliculus (SC). In neonatal rats, labeled axons originating from ... More
Electron microscopic visualization of receptor-mediated endocytosis of Dil-labeled lipoproteins by diaminobenzidine photoconversion.
AuthorsDantuma NP, Pijnenburg MA, Diederen JH, Van der Horst DJ
JournalJ Histochem Cytochem
PubMed ID9705975
'We present a modified diaminobenzidine (DAB) photoconversion method that enables staining of internalized Dil-labeled lipoproteins without the apparent punctate background staining that was observed with the original DAB photoconversion method. This is illustrated by the localization of Dil-labeled insect lipoproteins in natural recipient cells that internalize these lipoproteins by receptor-mediated ... More
Promotion of lymphocyte growth by high density lipoproteins (HDL). Physiological significance of the HDL binding site.
'The characteristics and physiological relevance of the high density lipoprotein (HDL) binding site on unstimulated and mitogen activated human peripheral blood lymphocytes have been investigated. At 37 degrees C, specific binding/uptake of fluorescent (dioctadecylin-docarbocyanine, DiI) HDL was observed by cells from healthy donors as well as by those from low ... More
Distinct roles for sodium, chloride, and calcium in excitotoxic dendritic injury and recovery.
AuthorsHasbani MJ, Hyrc KL, Faddis BT, Romano C, Goldberg MP
JournalExp Neurol
PubMed ID9875285
'The postsynaptic neuronal dendrite is selectively vulnerable to hypoxic-ischemic brain injury and glutamate receptor overactivation. We explored the glutamate receptor pharmacology and ionic basis of rapid, reversible alterations in dendritic shape which occur in cultured neurons exposed to glutamate. Dendrite morphology was assessed with the fluorescent membrane tracer, DiI, or ... More
Formation of projection pathways from the arcuate nucleus of the hypothalamus to hypothalamic regions implicated in the neural control of feeding behavior in mice.
AuthorsBouret SG, Draper SJ, Simerly RB
JournalJ Neurosci
PubMed ID15028773
'The arcuate nucleus of the hypothalamus (ARH) is a critical component of forebrain pathways that regulate a variety of neuroendocrine functions, including an important role in relaying leptin signals to other parts of the hypothalamus. However, neonatal rodents do not lose weight in response to leptin treatment in the same ... More
Heterogeneous proliferative potential in regenerative adult newt cardiomyocytes.
AuthorsBettencourt-Dias M, Mittnacht S, Brockes JP
JournalJ Cell Sci
PubMed ID12928330
'Adult newt cardiomyocytes, in contrast to their mammalian counterparts, can proliferate after injury and contribute to the functional regeneration of the heart. In order to understand the mechanisms underlying this plasticity we performed longitudinal studies on single cardiomyocytes in culture. We find that the majority of cardiomyocytes can enter S ... More
Assessment of factors regulating axon growth between the cortex and spinal cord in organotypic co-cultures: effects of age and neurotrophic factors.
AuthorsOishi Y, Baratta J, Robertson RT, Steward O
JournalJ Neurotrauma
PubMed ID15115608
'Axon growth failure in the central nervous system (CNS) of adult animals is thought to be attributable to several factors, including an inadequate intrinsic growth response, the presence of inhibitory molecules, and a lack of adequate neurotrophic support. Here we use a new in vitro assay system to quantitatively assess ... More
Measurement of rotational motion in membranes using fluorescence recovery after photobleaching.
AuthorsSmith LM, Weis RM, McConnell HM
JournalBiophys J
PubMed ID7284556
'A method has been developed for the measurement of the rotational motion of membrane components. In this method fluorescent molecules whose transition dipole moments lie in a given direction are preferentially destroyed with a short intense burst of polarized laser radiation. The fluorescence intensity, excited with a low intensity observation ... More
Structural and functional recovery from early monocular deprivation in adult rats.
AuthorsPizzorusso T, Medini P, Landi S, Baldini S, Berardi N, Maffei L
JournalProc Natl Acad Sci U S A
PubMed ID16709670
'Visual deficits caused by abnormal visual experience during development are hard to recover in adult animals. Removal of chondroitin sulfate proteoglycans from the mature extracellular matrix with chondroitinase ABC promotes plasticity in the adult visual cortex. We tested whether chondroitinase ABC treatment of adult rats facilitates anatomical, functional, and behavioral ... More
Developmental changes in phosphorylation state of neurofilament proteins in the chick embryonic optic nerve.
AuthorsGo MJ, Tanaka H, Obata K, Fujita SC
JournalDev Biol
PubMed ID2659412
'Developmental changes in the phosphorylation state of neurofilament proteins (NFPs) in the chick embryonic optic nerve were histochemically and biochemically studied using monoclonal antibody (MAb) 82E10 specific to the highly phosphorylated components of high (180K)- and middle (160K)-molecular-weight subunits of neurofilament (NF) in the chicken. Cross sections of developing embryonic ... More
Matching neural morphology to molecular expression: single cell injection following immunostaining.
AuthorsKao YH, Sterling P
JournalJ Neurocytol
PubMed ID14724387
'To match a neuron''s morphology with its expression of a particular protein, it is useful to first identify the cell by immunostaining and then inject it with fluorescent dye. Such targeted injection cannot be performed with a hydrophilic dye (such as Lucifer yellow) because the neuron, once rendered porous to ... More
Voltage-dependent translocation of R18 and DiI across lipid bilayers leads to fluorescence changes.
AuthorsMelikyan GB, Deriy BN, Ok DC, Cohen FS
JournalBiophys J
PubMed ID8913605
'We show that the lipophilic, cationic fluorescent dyes R18 and Dil translocate from one monolayer of a phospholipid bilayer membrane to the other in a concentration and voltage-dependent manner. When the probes were incorporated into voltage-clamped planar membranes and potentials were applied, displacement currents resulted. The charged probes sensed a ... More
Morphogenesis of chicken liver: identification of localized growth zones and the role of beta-catenin/Wnt in size regulation.
AuthorsSuksaweang S, Lin CM, Jiang TX, Hughes MW, Widelitz RB, Chuong CM
JournalDev Biol
PubMed ID14729482
'During development and regeneration, new cells are added and incorporated to the liver parenchyma. Regulation of this process contributes to the final size and shape of the particular organs, including the liver. We identified the distribution of liver growth zones using an embryonic chicken model because of its accessibility to ... More
Molecular mechanisms of erythrophagocytosis. Characterization of the senescent erythrocytes that are phagocytized by macrophages.
'We have recently developed a flow cytometric assay for the quantitation of erythrophagocytosis, using PKH 26-labeled erythrocytes as the target cells. Using this assay we have shown that there is extensive phagocytosis of desialylated erythrocytes. Furthermore, we have demonstrated that it is the densest population of erythrocytes obtained on a ... More
A quantitative study of the segmental distribution of somitic cells in the developing chick limb bud using laser-scanning confocal microscopy.
AuthorsEwan KB, Everett AW
JournalJ Histochem Cytochem
PubMed ID8601694
'Our aim was to map the segmental distribution of somitic cells in the limb before and after the fusion of these cells into myotubes. Somitic cells of the brachial somites were labeled by injection of DiI and DiO into the somitoceles of embryonic Day 2 (E2) embryos. The quantitative distribution ... More
Redistribution of plasma-membrane surface molecules during formation of the Leishmania amazonensis-containing parasitophorous vacuole.
AuthorsHenriques C, de Souza W
JournalParasitol Res
PubMed ID10726992
'Leishmania amazonensis presents two developmental stages that gain access to the host macrophage through phagocytosis. The protozoan resides in a membrane-bound compartment, the parasitophorous vacuole (PV), which can fuse with the endocytic system. For evaluation of the parasite/host-cell interaction process and of PV biogenesis, the two parasite forms or host-cell ... More
Tracing neuronal connections in postmortem human hippocampal complex with the carbocyanine dye DiI.
AuthorsMufson EJ, Brady DR, Kordower JH
JournalNeurobiol Aging
PubMed ID1704107
'This report describes the ability of the carbocyanine dye DiI to trace hippocampal complex connections in a paraformaldehyde immersion-fixed human postmortem brain. Six months after the placement of DiI crystals into the hilus of the dentate gyrus, the CA1 hippocampal subfield and the lateral entorhinal cortex, 50-microns thick, vibratome cut ... More
Usefulness of PKHs for studying cell proliferation.
AuthorsBoutonnat J, Barbier M, Rousselle C, Muirhead KA, Mousseau M, Seigneurin D, Ronot X
JournalC R Acad Sci III
PubMed ID9879469
'Classical methods for proliferative assessment (such as tritiated thymidine or bromodeoxyuridine (BrdUrd) incorporation) need sample fixation. As an alternative, we have evaluated the use of a dye dilution method using PKH26 to determine the rate and extent of proliferation in cell lines. Flow cytometric analysis associated with modelling software makes ... More
Hematopoietic stem cell tracking in vivo: a comparison of short-term and long-term repopulating cells.
AuthorsLanzkron SM, Collector MI, Sharkis SJ
JournalBlood
PubMed ID10068664
'We have previously demonstrated that we could separate long-term repopulating stem cells from cells that provided radioprotection (short-term repopulating cells) on the basis of size and suggested that this might be due to the quiescent nature of long-term repopulating cells. To further define the activity of these populations, we used ... More
Control of topographic retinal axon branching by inhibitory membrane-bound molecules.
AuthorsRoskies AL, O'Leary DD
JournalScience
PubMed ID8047886
'Retinotopic map development in nonmammalian vertebrates appears to be controlled by molecules that guide or restrict retinal axons to correct locations in their targets. However, the retinotopic map in the superior colliculus (SC) of the rat is developed instead by a topographic bias in collateral branching and arborization. Temporal retinal ... More
Rapid fluorometric assay of LDL receptor activity by DiI-labeled LDL.
AuthorsStephan ZF, Yurachek EC
JournalJ Lipid Res
PubMed ID8381454
'DiI-LDL (3,3''-dioctadecylindocarbocyanine-low density lipoprotein) has been extensively used in morphological and microscopic studies of receptor-mediated metabolism of LDL in many cell lines. To date the use of this fluorescent probe in a quantitative assay of LDL receptor activity has not been widely used in studies with multiple samples due to ... More
The properties of hNT cells following transplantation into the subventricular zone of the neonatal forebrain.
AuthorsZigova T, Pencea V, Sanberg PR, Luskin MB
JournalExp Neurol
PubMed ID10785441
'Neurons derived from the human teratocarcinoma cell line (hNT) establish structural polarity and a fully mature phenotype following transplantation into the rodent brain. Here we describe the transplantation of hNT cells into the anterior part of neonatal subventricular zone (SVZa), which is a prolific region of neuronal progenitor cells. Ordinarily, ... More
Structural organization of interphase 3T3 fibroblasts studied by total internal reflection fluorescence microscopy.
AuthorsLanni F, Waggoner AS, Taylor DL
JournalJ Cell Biol
PubMed ID3980580
'We studied the laminar organization of 3T3 fibroblast cells growing on glass slides by use of total internal reflection illumination to excite fluorescence emission (TIRF) from labeled molecules and stained cellular compartments that are very close to the cell-substrate contact region. Mitochondria, distant from the contact regions and stained with ... More
Rapid labeling of neuronal populations by ballistic delivery of fluorescent dyes.
AuthorsGrutzendler J, Tsai J, Gan WB
JournalMethods
PubMed ID12695105
'Particle-mediated ballistic delivery of fluorescent dyes has been recently used to label neuronal populations in a rapid and efficient fashion. Here we describe detailed protocols for this technique as well as recent improvements in its implementation. This technique allows rapid labeling of entire neurons in a Golgi-like manner after membranes ... More
Distinct modes of floor plate induction in the chick embryo.
AuthorsPatten I, Kulesa P, Shen MM, Fraser S, Placzek M,
JournalDevelopment
PubMed ID12917296
'To begin to reconcile models of floor plate formation in the vertebrate neural tube, we have performed experiments aimed at understanding the development of the early floor plate in the chick embryo. Using real-time analyses of cell behaviour, we provide evidence that the principal contributor to the early neural midline, ... More
Interhemispheric asymmetries of the modular structure in human temporal cortex.
AuthorsGaluske RA, Schlote W, Bratzke H, Singer W
JournalScience
PubMed ID10988077
'Language-relevant processing of auditory signals is lateralized and involves the posterior part of Brodmann area 22. We found that the functional lateralization in this area was accompanied by interhemispheric differences in the organization of the intrinsic microcircuitry. Neuronal tract tracing revealed a modular network of long-range intrinsic connections linking regularly ... More
Real-time multi-wavelength fluorescence imaging of living cells.
AuthorsMorris SJ
JournalBiotechniques
PubMed ID2331370
'We describe a new real-time fluorescence video microscope design for capturing intensified images of cells containing dual wavelength "ratio" dyes or multiple dyes. The microscope will perform real-time capture of two separate fluorescence emission images simultaneously, improving the time resolution of spatial distribution of fluorescence to video frame rates (30 ... More
Changes in somal growth and dendritic patterns of the retinal ganglion cells in the chicks and chick embryos.
AuthorsChen Y, Hu M, Shibata H, Naito J
JournalJ Vet Med Sci
PubMed ID14600356
'Changes in the somal growth and dendritic patterns of retinal ganglion cells (RGCs) were studied in early chick embryos and post-hatching chicks by means of the retrograde axonal transport labeling with DiI. Branching patterns of the dendrites were relatively uniform on E8 (embryonic day 8) and became more complicated on ... More
The influenza haemagglutinin-induced fusion cascade: effects of target membrane permeability changes.
AuthorsBlumenthal R, Morris SJ
JournalMol Membr Biol
PubMed ID10332736
'To define the stages in influenza haemagglutinin (HA)-mediated fusion the kinetics of fusion between cell pairs consisting of single influenza HA-expressing cells and single erythrocytes (RBC) which had been labelled with both a fluorescent lipid (Dil) in the membrane and a fluorescent solute (calcein) in the aqueous space have been ... More
Isolated rat cortical progenitor cells are maintained in division in vitro by membrane-associated factors.
AuthorsTemple S, Davis AA
JournalDevelopment
PubMed ID7600974
'Ventricular zone cells in the developing CNS undergo extensive cell division in vivo and under certain conditions in vitro. The culture conditions that promote cell division have been studied to determine the role that contact with cell membrane associated factors play in the proliferation of these cells. Progenitor cells have ... More