A fluorogenic substrate for the continuous assaying of aryl sulfatases.
AuthorsAhmed V, Ispahany M, Ruttgaizer S, Guillemette G, Taylor SD,
JournalAnal Biochem
PubMed ID15802133
'The most common fluorogenic substrate for assaying aryl sulfatases (ARSs) is 4-methylumbelliferyl sulfate (MUS). However, ARSs operate optimally at pH values that are less than the pK(a) (7.8) of the reaction product of MUS, 4-methylumbelliferone (4-MU). Thus, a major disadvantage of this assay is that it is usually run in ... More
Synthesis of novel fluorinated coumarins: excellent UV-light excitable fluorescent dyes.
AuthorsSun WC, Gee KR, Haugland RP
JournalBioorg Med Chem Lett
PubMed ID9873685
'Two new fluorinated fluorescent dyes, 6,8-difluoro-7-hydroxy-4-methylcoumarin (Marina Blue) and 3-carboxy-6,8-difluoro-7-hydroxycoumarin (Pacific Blue), exhibit excellent photophysical properties among a series of novel fluorinated 7-hydroxycoumarins. Most of these fluorinated coumarins have quantum yields (0.63 to 0.89) equal to or higher than that of the parent compound (0.63), which, in combination with their ... More
Characterization of the histidine-containing phosphotransfer protein B-mediated multistep phosphorelay system in Pseudomonas aeruginosa PAO1.
AuthorsHsu JL, Chen HC, Peng HL, Chang HY,
JournalJ Biol Chem
PubMed ID18256026
Certain bacterial two-component sensor kinases possess a histidine-containing phosphotransfer (Hpt) domain to carry out a multistep phosphotransferring reaction to a cognate response regulator. Pseudomonas aeruginosa PAO1 contains three genes that encode proteins with an Hpt domain but lack a kinase domain. To identify the sensor kinase coupled to these Hpt ... More
A fluorogenic substrate for detection of organophosphatase activity.
AuthorsSoukharev S, Hammond DJ
JournalAnal Biochem
PubMed ID15033522
A new fluorogenic substrate for the specific detection of organophosphatase (OPase) activity has been designed and evaluated. Our results indicate that 7-diethylphospho-6,8-difluor-4-methylumbelliferyl (DEPFMU) is hydrolyzed specifically by the OPases, mammalian serum paraoxonase and bacterial organophosphorus hydrolase (OPH). The apparent K(m) of DEPFMU is 29 microM for OPH and 91 and ... More