Jun NH2-terminal kinase (JNK) prevents nuclear beta-catenin accumulation and regulates axis formation in Xenopus embryos.
AuthorsLiao G, Tao Q, Kofron M, Chen JS, Schloemer A, Davis RJ, Hsieh JC, Wylie C, Heasman J, Kuan CY
JournalProc Natl Acad Sci U S A
PubMed ID17060633
'Jun NH(2)-terminal kinases (JNKs) regulate convergent extension movements in Xenopus embryos through the noncanonical Wnt/planar cell polarity pathway. In addition, there is a high level of maternal JNK activity spanning from oocyte maturation until the onset of gastrulation that has no defined functions. Here, we show that maternal JNK activation ... More
VDAC-dependent permeabilization of the outer mitochondrial membrane by superoxide induces rapid and massive cytochrome c release.
AuthorsMadesh M, Hajnóczky G
JournalJ Cell Biol
PubMed ID11739410
'Enhanced formation of reactive oxygen species (ROS), superoxide (O2*-), and hydrogen peroxide (H2O2) may result in either apoptosis or other forms of cell death. Here, we studied the mechanisms underlying activation of the apoptotic machinery by ROS. Exposure of permeabilized HepG2 cells to O2*- elicited rapid and massive cytochrome c ... More
One-step cellular caspase-3/7 assay.
AuthorsCarrasco RA, Stamm NB, Patel BK
JournalBiotechniques
PubMed ID12765032
Protease involvement in fodrin cleavage and phosphatidylserine exposure in apoptosis.
AuthorsVanags DM, Pörn-Ares MI, Coppola S, Burgess DH, Orrenius S
JournalJ Biol Chem
PubMed ID8940103
'A detailed kinetic analysis of three extranuclear end points of apoptosis, phosphatidylserine exposure, alpha-fodrin degradation, and plasma membrane blebbing, was performed and compared with nuclear fragmentation and the activation of the interleukin-1beta-converting enzyme (ICE)-like proteases in Jurkat T lymphocytes stimulated by anti-Fas monoclonal antibody (anti-Fas mAb) and in monocytic U937 ... More
Activation of the CED3/ICE-related protease CPP32 in cerebellar granule neurons undergoing apoptosis but not necrosis.
AuthorsArmstrong RC, Aja TJ, Hoang KD, Gaur S, Bai X, Alnemri ES, Litwack G, Karanewsky DS, Fritz LC, Tomaselli KJ
JournalJ Neurosci
PubMed ID8987778
'Neuronal apoptosis occurs during nervous system development and after pathological insults to the adult nervous system. Inhibition of CED3/ICE-related proteases has been shown to inhibit neuronal apoptosis in vitro and in vivo, indicating a role for these cysteine proteases in neuronal apoptosis. We have studied the activation of the CED3/ICE-related ... More
Heavy membrane-associated caspase 3: identification, isolation, and characterization.
AuthorsKrebs JF, Srinivasan A, Wong AM, Tomaselli KJ, Fritz LC, Wu JC
JournalBiochemistry
PubMed ID11123933
'Heavy membrane preparations from 697 lymphoblastoid cells contain a tightly bound caspase zymogen. This heavy membrane-bound procaspase can be efficiently liberated from membrane preparations using detergents. Alternatively, the procaspase can be rapidly processed and activated from membrane preparations by caspase-1 without detergents. The activated caspase-3 was purified using affinity chromatography ... More
IRF-1 is an essential mediator in IFN-gamma-induced cell cycle arrest and apoptosis of primary cultured hepatocytes.
AuthorsKano A, Haruyama T, Akaike T, Watanabe Y
JournalBiochem Biophys Res Commun
PubMed ID10208842
'IFN-gamma induces cell cycle arrest and p53-independent apoptosis in primary cultured hepatocytes. However, it is not yet understood what molecules regulate the mechanism. We report here that interferon regulatory factor 1 (IRF-1) is an essential molecule in these phenomena. Hepatocytes from IRF-1-deficient mice were completely resistant to IFN-gamma in apoptosis ... More
Changes in cytosolic Ca2+ levels regulate Bcl-xS and Bcl-xL expression in spermatogenic cells during apoptotic death.
AuthorsMishra DP, Pal R, Shaha C
JournalJ Biol Chem
PubMed ID16301318
'Bcl-x exists in two isoforms, the anti-apoptotic form Bcl-xL and the proapoptotic form Bcl-xS. The critical balance between the two forms appears to be important for cell survival; however, it is still not clear exactly how the vital balance is maintained. Using an in vitro spermatogenic cell apoptosis model, this ... More
Cystatin A expression reduces bile salt-induced apoptosis in a rat hepatoma cell line.
AuthorsJones B, Roberts PJ, Faubion WA, Kominami E, Gores GJ
JournalAm J Physiol
PubMed ID9756503
'We have previously demonstrated abrogation of bile salt-induced apoptosis by cathepsin B inhibitors. However, caspases have been strongly implicated in apoptosis, and the mechanistic interface between caspase and cathepsin B activation is unclear. Thus our aims were to determine the mechanistic relationship between caspases and cathepsin B in bile salt-induced ... More
Dual effect of glucocorticoids on apoptosis of human autoreactive and foreign antigen-specific T cells.
AuthorsZipp F, Wendling U, Beyer M, Grieger U, Waiczies S, Wagenknecht B, Haas J, Weller M
JournalJ Neuroimmunol
PubMed ID11024552
'The efficacy of glucocorticoids in the treatment of multiple sclerosis may involve the induction of T cell apoptosis. Here, we report that glucocorticoids have two different effects on the vulnerability of human antigen-specific T cells: (i) steroids induce T cell apoptosis in a CD95-independent, but caspase-dependent manner; (ii) steroids protect ... More
Mch2, a new member of the apoptotic Ced-3/Ice cysteine protease gene family.
AuthorsFernandes-Alnemri T, Litwack G, Alnemri ES
JournalCancer Res
PubMed ID7796396
'We have developed a PCR approach to clone new apoptotic Ced-3/Ice-like cysteine protease genes. This approach uses degenerate oligonucleotides encoding the highly conserved pentapeptides QACRG and GSWFI that are present in all known apoptotic cysteine proteases. Using this approach, we have cloned a novel apoptotic gene from human Jurkat T ... More
Caspase involvement in the induction of apoptosis by the environmental toxicants tributyltin and triphenyltin.
AuthorsStridh H, Orrenius S, Hampton MB
JournalToxicol Appl Pharmacol
PubMed ID10198279
'Organotin compounds such as tributyltin (TBT) and triphenyltin (TPT) can kill target cells by triggering apoptosis. The mechanism by which these environmental toxicants activate the apoptotic program is currently unclear. We have studied the effect of TBT and TPT in the human Hut-78 and Jurkat T-lymphocyte cell lines. Within 1 ... More
Room temperature-induced apoptosis of Jurkat cells sensitive to both caspase-1 and caspase-3 inhibitors.
AuthorsShimura M, Okuma E, Yuo A, Sasaki T, Mukai C, Takaku F, Ishizaka Y
JournalCancer Lett
PubMed ID10397447
'We recently reported that HL-60 cells underwent apoptosis when exposed to room temperature (RT) (21 degrees C). RT-induced apoptosis of HL-60 cells is inhibited by the caspase-1 inhibitor (YVAD-CMK), but not by the caspase-3 inhibitor (DEVD-CHO). In this study, we studied RT-induced apoptosis in 15 human cell lines of hematopoietic ... More
Requirements for proteolysis during apoptosis.
AuthorsVaux DL, Wilhelm S, Häcker G
JournalMol Cell Biol
PubMed ID9343413
'The key effector proteins of apoptosis are a family of cysteine proteases termed caspases. Following activation of caspases, biochemical events occur that lead to DNA degradation and the characteristic morphological changes associated with apoptosis. Here we show that cytoplasmic extracts activated in vitro by proteinase K were able to cleave ... More
Cytochrome c binds to inositol (1,4,5) trisphosphate receptors, amplifying calcium-dependent apoptosis.
AuthorsBoehning D, Patterson RL, Sedaghat L, Glebova NO, Kurosaki T, Snyder SH
JournalNat Cell Biol
PubMed ID14608362
'Mitochondrial cytochrome c release and inositol (1,4,5) trisphosphate receptor (InsP(3)R)-mediated calcium release from the endoplasmic reticulum mediate apoptosis in response to specific stimuli. Here we show that cytochrome c binds to the InsP(3)R during apoptosis. Addition of 1 nM cytochrome c blocks calcium-dependent inhibition of InsP(3)R function. Early in apoptosis, ... More
Inhibition of caspase-3 by S-nitrosation and oxidation caused by nitric oxide.
AuthorsMohr S, Zech B, Lapetina EG, Brüne B
JournalBiochem Biophys Res Commun
PubMed ID9299518
'Apoptotic signaling cascades converge in the activation of caspases (interleukin-1beta converting enzyme like proteases). Treatment of the human promyelocytic leukaemia cell line U937 with actinomycin D resulted in the activation of caspase-3 also known as CPP32. Protease activity was measured in cytosolic extracts by fluorometric analysis of the time-dependent cleavage ... More
Sensitization to CD95 ligand-induced apoptosis in human glioma cells by hyperthermia involves enhanced cytochrome c release.
AuthorsHermisson M, Wagenknecht B, Wolburg H, Glaser T, Dichgans J, Weller M
JournalOncogene
PubMed ID10822385
'CD95L-induced apoptosis involves caspase activation and is facilitated when RNA and protein synthesis are inhibited. Here, we report that hyperthermia sensitizes malignant glioma cells to CD95L- and APO2L-induced apoptosis in the absence, but not in the presence, of inhibitors of RNA and protein synthesis. Hyperthermia does not alter CD95 expression ... More
The presenilin-2 loop peptide perturbs intracellular Ca2+ homeostasis and accelerates apoptosis.
AuthorsCai C, Lin P, Cheung KH, Li N, Levchook C, Pan Z, Ferrante C, Boulianne GL, Foskett JK, Danielpour D, Ma J
JournalJ Biol Chem
PubMed ID16603547
'In cells undergoing apoptosis, a 22-amino-acid presenilin-2-loop peptide (PS2-LP, amino acids 308-329 in presenilin-2) is generated through cleavage of the carboxyl-terminal fragment of presenilin-2 by caspase-3. The impact of PS2-LP on the progression of apoptosis, however, is not known. Here we show that PS2-LP is a potent inducer of the ... More
Detection of caspase-activation in intact lymphoid cells using standard caspase substrates and inhibitors.
AuthorsMack A, Fürmann C, Häcker G
JournalJ Immunol Methods
PubMed ID10915846
'Members of the caspase family of proteases are important in the implementation of apoptotic cell death. These caspases are intracellularly activated upon a death stimulus, and exhibit a distinctive proteolytic activity which transmits a death signal and readily detected by measuring the cleavage of synthetic substrates in cell extracts. In ... More
Substance P promotes expansion of human mesenteric preadipocytes through proliferative and antiapoptotic pathways.
AuthorsGross K, Karagiannides I, Thomou T, Koon HW, Bowe C, Kim H, Giorgadze N, Tchkonia T, Pirtskhalava T, Kirkland JL, Pothoulakis C,
JournalAm J Physiol Gastrointest Liver Physiol
PubMed ID19282377
'White adipose tissue is intimately involved in the regulation of immunity and inflammation. We reported that human mesenteric preadipocytes express the substance P (SP)-mediated neurokinin-1 receptor (NK-1R), which signals proinflammatory responses. Here we tested the hypothesis that SP promotes proliferation and survival of human mesenteric preadipocytes and investigated responsible mechanism(s). ... More
Regulation of apoptosis by BH3 domains in a cell-free system.
AuthorsCosulich SC, Worrall V, Hedge PJ, Green S, Clarke PR
JournalCurr Biol
PubMed ID9382837
'BACKGROUND: The Bcl-2 family of proteins plays a key role in the regulation of apoptosis. Some family members prevent apoptosis induced by a variety of stimuli, whereas others promote apoptosis. Competitive dimerisation between family members is thought to regulate their function. Homologous domains within individual proteins are necessary for interactions ... More
Isoelectric focusing and enzyme overlay membrane analysis of caspase 3 activation.
AuthorsBreithaupt TB, Shires AL, Voehringer DW, Herzenberg LA, Herzenberg LA
JournalAnal Biochem
PubMed ID11355870
No induction of apoptosis by IFN-beta in human antigen-specific T cells.
AuthorsZipp F, Beyer M, Gelderblom H, Wernet D, Zschenderlein R, Weller M
JournalNeurology
PubMed ID10668719
Interferon (IFN)-beta, the most effective immunomodulatory treatment for MS, inhibits the proliferation of myelin-specific T cells. We report that IFN-beta moderately enhances the expression of the death receptor, CD95, at the surface of human antigen-specific T cells. However, T-cell apoptosis was not induced by IFNbeta-1a or IFNbeta-1b as assessed by ... More
Inhibition of etoposide-induced apoptotic events by azide.
AuthorsWilhelm S, Roloff S, Häcker G
JournalImmunol Lett
PubMed ID9334858
Azide is reported to be an inducer of necrotic rather than apoptotic cell death. Using various parameters of cell death, we demonstrate here that azide is capable of completely inhibiting apoptosis induced by VP-16/etoposide. Azide was found systematically to protect Jurkat cells against VP-16-induced effects as determined by an array ... More
Identification of BARD1 as mediator between proapoptotic stress and p53-dependent apoptosis.
AuthorsIrminger-Finger I, Leung WC, Li J, Dubois-Dauphin M, Harb J, Feki A, Jefford CE, Soriano JV, Jaconi M, Montesano R, Krause KH
JournalMol Cell
PubMed ID11779501
The BRCA1-associated protein BARD1 is a putative tumor suppressor. We suggest that BARD1 is a mediator of apoptosis since (1) cell death in vivo (ischemic stroke) and in vitro is accompanied by increased levels of BARD1 protein and mRNA; (2) overexpression of BARD1 induces cell death with all features of ... More
Role of the executioner caspases during lens development.
The notion that the cell death machinery is utilized during lens organelle degradation is supported by the observation that well characterized apoptotic substrates are cleaved during this process. Here, we test directly the role of executioner caspases (caspase-3, -6, and -7) in fiber cell differentiation. The distribution of mRNA, protein, ... More
Neisseria gonorrhoeae infection protects human endocervical epithelial cells from apoptosis via expression of host antiapoptotic proteins.
AuthorsFollows SA, Murlidharan J, Massari P, Wetzler LM, Genco CA,
JournalInfect Immun
PubMed ID19546192
Several microbial pathogens can modulate the host apoptotic response to infection, which may contribute to immune evasion. Various studies have reported that infection with the sexually transmitted disease pathogen Neisseria gonorrhoeae can either inhibit or induce apoptosis. N. gonorrhoeae infection initiates at the mucosal epithelium, and in women, cells from ... More
Extracellular adenosine-induced apoptosis in mouse neuroblastoma cells: studies on involvement of adenosine receptors and adenosine uptake.
AuthorsSchrier SM, van Tilburg EW, van der Meulen H, Ijzerman AP, Mulder GJ, Nagelkerke JF
JournalBiochem Pharmacol
PubMed ID11226375
The induction of apoptosis by adenosine was studied in the mouse neuroblastoma cell line N1E-115. Apoptosis was characterized by fluorescence and electron microscopy, fluorescence-activated cell sorter (FACS) analysis, and caspase activity assays. A sixteen-hour exposure to 100 microM of adenosine led to chromatin condensation and caspase activation. However, selective agonists ... More
Cytochrome c is dispensable for fas-induced caspase activation and apoptosis.
AuthorsVier J, Linsinger G, Häcker G
JournalBiochem Biophys Res Commun
PubMed ID10405325
Cytochrome c is thought to play an important role in the initiation of apoptosis following its release from mitochondria. It is controversial whether such release is also involved in caspase activation and apoptotic cell death after ligation of the cell surface molecule Fas. We addressed this issue by investigating cells ... More
Acceleration of apoptotic cell death after the cleavage of Bcl-XL protein by caspase-3-like proteases.
AuthorsFujita N, Nagahashi A, Nagashima K, Rokudai S, Tsuruo T
JournalOncogene
PubMed ID9771973
Interleukin-2 (IL-2)-dependent T cell clone CTLL-2 underwent apoptosis by deprivation of IL-2 from culture medium. The decrease in the anti-apoptotic Bcl-XL protein level was observed during apoptosis after IL-2 withdrawal. We found that Bcl-XL protein was cleaved to produce two 18 kDa fragments during CTLL-2 cell apoptosis. When the activation ... More
Apoptotic crosstalk between the endoplasmic reticulum and mitochondria controlled by Bcl-2.
AuthorsHäcki J, Egger L, Monney L, Conus S, Rossé T, Fellay I, Borner C
JournalOncogene
PubMed ID10822379
Apoptosis involves mitochondrial steps such as the release of the apoptogenic factor cytochrome c which are effectively blocked by Bcl-2. Although Bcl-2 may have a direct action on the mitochondrial membrane, it also resides and functions on the endoplasmic reticulum (ER), and there is increasing evidence for a role of ... More
Caspase inhibitor affords neuroprotection with delayed administration in a rat model of neonatal hypoxic-ischemic brain injury.
AuthorsCheng Y, Deshmukh M, D'Costa A, Demaro JA, Gidday JM, Shah A, Sun Y, Jacquin MF, Johnson EM, Holtzman DM
JournalJ Clin Invest
PubMed ID9576764
Programmed cell death (apoptosis) is a normal process in the developing nervous system. Recent data suggest that certain features seen in the process of programmed cell death may be favored in the developing versus the adult brain in response to different brain injuries. In a well characterized model of neonatal ... More
Induction of apoptosis by SB202190 through inhibition of p38beta mitogen-activated protein kinase.
AuthorsNemoto S, Xiang J, Huang S, Lin A
JournalJ Biol Chem
PubMed ID9632706
p38, a subfamily of the mitogen-activated protein kinase, regulates gene expression in response to various extracellular stimuli. The pyridinyl imidazoles like SB202190 are specific inhibitors of p38alpha and p38beta and have been widely used in investigation of the biological functions of p38. Here we show that SB202190 by itself was ... More
Human oligodendroglial cell line, MO3.13, can be protected from apoptosis using the general caspase inhibitor zVAD-FMK.
AuthorsCraighead MW, Tiwari P, Keynes RG, Waters CM
JournalJ Neurosci Res
PubMed ID10398301
Recent evidence suggests that the oligodendrocyte cell loss observed in multiple sclerosis sufferers is in part mediated by apoptosis. Here we use a human cell line, MO3.13, as a model system to investigate the biochemical processes involved in oligodendroglial cell death. Treatment with staurosporine kills both naive and differentiated cells ... More
Dihydroheptaprenyl and dihydrodecaprenyl monophosphates induce apoptosis mediated by activation of caspase-3-like protease.
AuthorsYasugi E, Nakata K, Yokoyama Y, Kano K, Dohi T, Oshima M
JournalBiochim Biophys Acta
PubMed ID9461254
Dolichyl phosphate, an essential carrier lipid in the biosynthesis of N-linked glycoprotein, has been found to induce apoptosis in rat glioma C6 cells and human monoblastic leukemia U937 cells. In the present study, dolichyl phosphate and structurally related compounds were examined regarding their apoptosis-inducing activities in U937 cells. Dihydroheptaprenyl and ... More
Pivotal role of reactive oxygen species as intracellular mediators of hyperthermia-induced apoptosis.
The effects of cellular antioxidant capacity on hyperthermia (HT)-induced apoptosis and production of antiapoptotic heat shock proteins (HSPs) were investigated in HL-60 cells and in HL-60AR cells that are characterized by an elevated endogenous catalase activity. Exposure of both cell lines to 43 degrees C for 1 h initiated apoptosis. ... More
p38 Mitogen-activated protein kinase and phosphatidylinositol 3-kinase activities have opposite effects on human neutrophil apoptosis.
AuthorsAlvarado-Kristensson M, Porn-Ares MI, Grethe S, Smith D, Zheng L, Andersson T
JournalFASEB J
PubMed ID11729103
Neutrophil apoptosis is essential for resolution of inflammatory reactions. Here, we studied the role of two apoptosis/survival-associated protein kinases in this process. We discovered a previously undetected early and transient inhibition of the activity of p38 mitogen-activated protein kinase (p38 MAPK) during both spontaneous and Fas-induced apoptosis. Pharmacological inhibition of ... More
Caspase activation is an early event in anthracycline-induced apoptosis and allows detection of apoptotic cells before they are ingested by phagocytes.
An increasing number of methods are being described to detect apoptotic cells. However, attempts to detect apoptotic cells in clinical samples are rarely successful. A hypothesis is that apoptotic cells are cleared from the circulation by phagocytosis before they become detectable by conventional morphological or cytometric methods. Using LR73 adhering ... More
Induction of apoptosis and caspase activation in cells obtained from familial haemophagocytic lymphohistiocytosis patients.
AuthorsFadeel B, Orrenius S, Henter JI
JournalBr J Haematol
PubMed ID10460599
Familial haemophagocytic lymphohistiocytosis (FHL) is a rare and uniformly fatal disorder of early childhood characterized by fever, hepatosplenomegaly, cytopenia and widespread infiltration of vital organs by activated lymphocytes and macrophages. In order to test whether the massive accumulation of immune cells in these patients is associated with a perturbation of ... More
Inhibition of CD95/Fas-induced DNA degradation by osmotic cell shrinkage.
AuthorsUhlemann AC, Müller C, Madlung J, Gulbins E, Lang F
JournalCell Physiol Biochem
PubMed ID11093032
Apoptosis (programmed cell death) is an active physiological mechanism from which removal of abundant or potentially harmful cells follows. Apoptosis of lymphocytes is critical for the development of the immune system and during the immune response. As we have shown previously, moderate osmotic cell shrinkage interferes with CD95(Fas/Apo-1)-induced cell death. ... More
Cascades of mammalian caspase activation in the yeast Saccharomyces cerevisiae.
AuthorsKang JJ, Schaber MD, Srinivasula SM, Alnemri ES, Litwack G, Hall DJ, Bjornsti MA
JournalJ Biol Chem
PubMed ID9915859
Caspases (aspartate-specific cysteine proteases) play a critical role in the execution of the mammalian apoptotic program. To address the regulation of human caspase activation, we used the yeast Saccharomyces cerevisiae, which is devoid of endogenous caspases. The apical procaspases, -8beta and -10, were efficiently processed and activated in yeast. Although ... More
Jasplakinolide induces apoptosis in various transformed cell lines by a caspase-3-like protease-dependent pathway.
AuthorsOdaka C, Sanders ML, Crews P
JournalClin Diagn Lab Immunol
PubMed ID11063504
To clarify the mechanisms underlying the antiproliferative effects of jasplakinolide, a cyclic depsipeptide from marine sponges, we examined whether jasplakinolide induces apoptosis in a variety of transformed and nontransformed cells. Jasplakinolide inhibited proliferation of human Jurkat T cells, resulting in cell death. This was accompanied by chromatin condensation and DNA ... More
The apoptosis-necrosis paradox. Apoptogenic proteases activated after mitochondrial permeability transition determine the mode of cell death.
AuthorsHirsch T, Marchetti P, Susin SA, Dallaporta B, Zamzami N, Marzo I, Geuskens M, Kroemer G
JournalOncogene
PubMed ID9380409
Mitochondrial alterations including permeability transition (PT) constitute critical events of the apoptotic cascade and are under the control of Bcl-2 related gene products. Here we show that induction of PT is sufficient to activate CPP32-like proteases with DEVDase activity and the associated cleavage of the nuclear DEVDase substrate poly(ADP-ribose) polymerase ... More
Protease activation and cleavage of poly(ADP-ribose) polymerase: an integral part of apoptosis in response to photodynamic treatment.
AuthorsHe J, Whitacre CM, Xue LY, Berger NA, Oleinick NL
JournalCancer Res
PubMed ID9500454
Apoptosis induced by numerous cancer chemotherapeutic and other toxic agents has been shown to proceed through a cascade of proteases, now termed caspases, culminating in cleavage of a set of proteins. The ability of photodynamic treatment (PDT) with the phthalocyanine Pc 4 to activate cellular caspases has been assessed during ... More
Changes of caspase activities involved in apoptosis of a macrophage-like cell line J774.1/JA-4 treated with lipopolysaccharide (LPS) and cycloheximide.
AuthorsKarahashi H, Amano F
JournalBiol Pharm Bull
PubMed ID10706374
The addition of lipopolysaccharide (LPS) together with cycloheximide (CHX) induced apoptosis in a subline of a J774.1 macrophage-like cell line, JA-4, as judged by terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL)-staining and poly(adenosine 5'-diphosphate (ADP)-ribose) polymerase (PARP)-cleavage. Caspase activities were examined in these macrophages in vitro ... More
Multiple intracellular pathways interfere with the activation of a CPP32-like protease induced by serum deprivation of AKR-2B cells.
AuthorsSchäfer R, Karbach D, Hoppe J
JournalExp Cell Res
PubMed ID9570918
As previously described, confluent AKR-2B fibroblasts rapidly disintegrate upon removal of serum. Platelet-derived growth factor isoforms AB or BB (PDGF-AB, -BB) added immediately after serum deprivation caused complete survival of the cells without initiating proliferation (Simm et al., 1994, J. Cell. Physiol. 160, 295). Here the role of cAMP as ... More
Bax deletion further orders the cell death pathway in cerebellar granule cells and suggests a caspase-independent pathway to cell death.
AuthorsMiller TM, Moulder KL, Knudson CM, Creedon DJ, Deshmukh M, Korsmeyer SJ, Johnson EM
JournalJ Cell Biol
PubMed ID9314540
Dissociated cerebellar granule cells maintained in medium containing 25 mM potassium undergo an apoptotic death when switched to medium with 5 mM potassium. Granule cells from mice in which Bax, a proapoptotic Bcl-2 family member, had been deleted, did not undergo apoptosis in 5 mM potassium, yet did undergo an ... More
Apoptosis in hematopoietic cells (FL5.12) caused by interleukin-3 withdrawal: relationship to caspase activity and the loss of glutathione.
AuthorsBojes HK, Feng X, Kehrer JP, Cohen GM
JournalCell Death Differ
PubMed ID10200549
The mechanism of cell death caused by cytokine deprivation remains largely unknown. FL5.12 cells (a murine prolymphocytic cell line), following interleukin-3 (IL-3) withdrawal, undergo a decrease in intracellular glutathione (GSH) that precedes the onset of apoptosis. In the present study, the induction of apoptosis following IL-3 withdrawal or GSH depletion ... More
Characterization of CPP32-like protease activity following apoptotic challenge in SH-SY5Y neuroblastoma cells.
AuthorsPosmantur R, McGinnis K, Nadimpalli R, Gilbertsen RB, Wang KK
JournalJ Neurochem
PubMed ID9166725
We characterized the activation of interleukin-1beta-converting enzyme (ICE)-like proteases (caspases) in human neuroblastoma cells (SH-SY5Y) following challenge with staurosporine, an established agent known to induce apoptosis. Time course analyses of lactate dehydrogenase release detected a significant increase in cell death as early as 6 h that continued at least until ... More
Suppression of apoptosis by glucocorticoids in glomerular endothelial cells: effects on proapoptotic pathways.
AuthorsMessmer UK, Winkel G, Briner VA, Pfeilschifter J
JournalBr J Pharmacol
PubMed ID10780973
Tumour necrosis factor-alpha (TNF-alpha)- and lipopolysaccharide (LPS)-induced apoptosis of bovine glomerular endothelial cells is now recognized as an important part in the pathogenesis of glomerulonephritis characterized by early mitochondrial cytochrome c release, mitochondrial permeability transition, Bak protein upregulation, Bcl-X(L) protein downregulation and caspase-3 activation. Co-treatment of cells with 10 nM ... More
Myxoma virus Serp2 is a weak inhibitor of granzyme B and interleukin-1beta-converting enzyme in vitro and unlike CrmA cannot block apoptosis in cowpox virus-infected cells.
The Serp2 protein encoded by the leporipoxvirus myxoma virus is essential for full virulence (F. Messud-Petit, J. Gelfi, M. Delverdier, M. F. Amardeilh, R. Py, G. Sutter, and S. Bertagnoli, J. Virol. 72:7830-7839, 1998) and, like crmA of cowpox virus (CPV), is reported to inhibit the interleukin-1beta-converting enzyme (ICE, caspase-1) ... More
G-protein signaling abnormalities mediated by CD95 in salivary epithelial cells.
AuthorsLiu XB, Masago R, Kong L, Zhang BX, Masago S, Vela-Roch N, Katz MS, Yeh CK, Zhang GH, Talal N, Dang H
JournalCell Death Differ
PubMed ID11139286
Salivary epithelial cells from patients with primary Sjögren's syndrome (SS) undergo Fas-mediated apoptosis. Bcl-2 and Bcl-xL are apoptosis suppressing oncogenes. Very little is known about the role of these oncogene molecules in salivary epithelial cells. To investigate the possible prevention of salivary glandular destruction in SS by Bcl-2 and Bcl-xL, ... More