EnzChek™ Caspase-3 Activity Assay Kits, 500 assays - FAQs

I am planning to use the EnzChek Caspase-3 Assay Kit #1 with Z-DEVD-AMC substrate. What are the fluorescence excitation/emission maxima of the cleaved substrate?

The basis for the EnzChek Caspase-3 Assay is the 7-amino-4-methylcoumarin-derived substrate Z-DEVD-AMC (where Z represents a benzyloxycarbonyl group), which is weakly fluorescent in the UV range (excitation/emission ~330/390 nm), but yields a bright blue fluorescent product (excitation/emission ~342/441 nm) upon proteolytic cleavage. Measure the fluorescence (excitation/emission ~342/441 nm) using appropriate excitation and emission filters or settings.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.