表皮生长因子、生物素化、与 Alexa Fluor™ 488 链霉亲和素(Alexa Fluor™ 488 EGF 复合物)的复合物
表皮生长因子、生物素化、与 Alexa Fluor™ 488 链霉亲和素(Alexa Fluor™ 488 EGF 复合物)的复合物
Invitrogen™

表皮生长因子、生物素化、与 Alexa Fluor™ 488 链霉亲和素(Alexa Fluor™ 488 EGF 复合物)的复合物

我们提供了几种表皮生长因子偶联物,这些偶联物对于检测细胞中的 EGF 受体非常有用。荧光标记的 EGF 使科学家能够考察受体-膜之间的相互作用,研究受体分布,计算 EGF 与受体相互作用的速率常数等。该偶联物含有荧光素 (FITC)了解更多信息
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货号数量
E13345100 μg
货号 E13345
价格(CNY)
7,847.00
Each
添加至购物车
数量:
100 μg
价格(CNY)
7,847.00
Each
添加至购物车
我们提供了几种表皮生长因子偶联物,这些偶联物对于检测细胞中的 EGF 受体非常有用。荧光标记的 EGF 使科学家能够考察受体-膜之间的相互作用,研究受体分布,计算 EGF 与受体相互作用的速率常数等。该偶联物含有荧光素 (FITC)、Oregon Green™ 514 和四甲基罗丹明,使用与 EGF 直接连接的染料构建。EGF 与 Alexa Fluor™ 染料和 Texas Red™ 染料的偶联物是染料–链霉亲和素分子和生物素化 EGF 的复合物。生物素化偶联物利用生物素-XX,它含有一个长间隔臂,旨在增强探针对 EGF 受体的亲和力。

表皮生长因子偶联物规格:
• EGF 分子:53 个氨基酸,MW=6,045 Da
• 标记(激发/发射波长):生物素-XX 与 Alexa Fluor™ 488 链霉素亲和素 (∼495/519 nm) 的复合物
• 每个 EGF 分子上的荧光基团分子数量:∼2–3 个
• 通常使用流式细胞仪、荧光显微镜或荧光计监测荧光信号


查找更多的受体结合和吞噬作用探针
我们提供多种用于研究受体介导的内吞作用的荧光标记探针,用于内吞作用和胞吐作用的膜标记物,以及检测内吞的荧光配体的方法。查看 Molecular Probes™ 手册中用于追踪受体结合和吞噬作用的探针—第 16.1 节,了解有关这些产品的更多信息。

供研究使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
细胞类型哺乳动物细胞
检测方法荧光
染料类型Alexa Fluor 染料
形式实心
蛋白质家族EGF
数量100 μg
运输条件湿冰
产品线Alexa Fluor
产品类型生长因子
Unit SizeEach
内容与储存
储存在冰箱(-5 至 -30°C)中并避光。

常见问题解答 (FAQ)

What is the molecular weight of Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor 488 Streptavidin (Alexa Fluor 488 EGF complex) (Cat. No. E13345)?

The Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor 488 Streptavidin (Alexa Fluor 488 EGF complex) (Cat. No. E13345) has an approximate MW of ~60 kDa.

Find additional tips, troubleshooting help, and resources within our Labeling Chemistry Support Center.

引用和文献 (19)

引用和文献
Abstract
Systems survey of endocytosis by multiparametric image analysis.
Authors:Collinet C, Stöter M, Bradshaw CR, Samusik N, Rink JC, Kenski D, Habermann B, Buchholz F, Henschel R, Mueller MS, Nagel WE, Fava E, Kalaidzidis Y, Zerial M,
Journal:Nature
PubMed ID:20190736
'Endocytosis is a complex process fulfilling many cellular and developmental functions. Understanding how it is regulated and integrated with other cellular processes requires a comprehensive analysis of its molecular constituents and general design principles. Here, we developed a new strategy to phenotypically profile the human genome with respect to transferrin ... More
The Ankrd 13 family of UIM-bearing proteins regulates EGF receptor endocytosis from the plasma membrane.
Authors:Tanno H, Yamaguchi T, Goto E, Ishido S, Komada M,
Journal:Mol Biol Cell
PubMed ID:22298428
'The mechanism of ubiquitin-dependent endocytosis of cell surface proteins is not completely understood. Here we examine the role of the ankyrin repeat domain (Ankrd) 13A, 13B, and 13D proteins, which constitute a functionally unknown family of ubiquitin-interacting motif (UIM)-bearing proteins, in the process. Stimulation of human HeLa cells with epidermal ... More
Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cells.
Authors:Varnai P, Thyagarajan B, Rohacs T, Balla T
Journal:J Cell Biol
PubMed ID:17088424
'Rapamycin (rapa)-induced heterodimerization of the FRB domain of the mammalian target of rapa and FKBP12 was used to translocate a phosphoinositide 5-phosphatase (5-ptase) enzyme to the plasma membrane (PM) to evoke rapid changes in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P(2)) levels. Rapa-induced PM recruitment of a truncated type IV 5-ptase containing only the ... More
Beclin1-binding UVRAG targets the class C Vps complex to coordinate autophagosome maturation and endocytic trafficking.
Authors:Liang C, Lee JS, Inn KS, Gack MU, Li Q, Roberts EA, Vergne I, Deretic V, Feng P, Akazawa C, Jung JU,
Journal:Nat Cell Biol
PubMed ID:18552835
'Autophagic and endocytic pathways are tightly regulated membrane rearrangement processes that are crucial for homeostasis, development and disease. Autophagic cargo is delivered from autophagosomes to lysosomes for degradation through a complex process that topologically resembles endosomal maturation. Here, we report that a Beclin1-binding autophagic tumour suppressor, UVRAG, interacts with the ... More
Hip1-related mutant mice grow and develop normally but have accelerated spinal abnormalities and dwarfism in the absence of HIP1.
Authors:Hyun TS, Li L, Oravecz-Wilson KI, Bradley SV, Provot MM, Munaco AJ, Mizukami IF, Sun H, Ross TS,
Journal:Mol Cell Biol
PubMed ID:15121852
In mice and humans, there are two known members of the Huntingtin interacting protein 1 (HIP1) family, HIP1 and HIP1-related (HIP1r). Based on structural and functional data, these proteins participate in the clathrin trafficking network. The inactivation of Hip1 in mice leads to spinal, hematopoietic, and testicular defects. To investigate ... More