Systems survey of endocytosis by multiparametric image analysis.
AuthorsCollinet C, Stöter M, Bradshaw CR, Samusik N, Rink JC, Kenski D, Habermann B, Buchholz F, Henschel R, Mueller MS, Nagel WE, Fava E, Kalaidzidis Y, Zerial M,
JournalNature
PubMed ID20190736
'Endocytosis is a complex process fulfilling many cellular and developmental functions. Understanding how it is regulated and integrated with other cellular processes requires a comprehensive analysis of its molecular constituents and general design principles. Here, we developed a new strategy to phenotypically profile the human genome with respect to transferrin ... More
The Ankrd 13 family of UIM-bearing proteins regulates EGF receptor endocytosis from the plasma membrane.
AuthorsTanno H, Yamaguchi T, Goto E, Ishido S, Komada M,
JournalMol Biol Cell
PubMed ID22298428
'The mechanism of ubiquitin-dependent endocytosis of cell surface proteins is not completely understood. Here we examine the role of the ankyrin repeat domain (Ankrd) 13A, 13B, and 13D proteins, which constitute a functionally unknown family of ubiquitin-interacting motif (UIM)-bearing proteins, in the process. Stimulation of human HeLa cells with epidermal ... More
Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact living cells.
AuthorsVarnai P, Thyagarajan B, Rohacs T, Balla T
JournalJ Cell Biol
PubMed ID17088424
'Rapamycin (rapa)-induced heterodimerization of the FRB domain of the mammalian target of rapa and FKBP12 was used to translocate a phosphoinositide 5-phosphatase (5-ptase) enzyme to the plasma membrane (PM) to evoke rapid changes in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P(2)) levels. Rapa-induced PM recruitment of a truncated type IV 5-ptase containing only the ... More
Beclin1-binding UVRAG targets the class C Vps complex to coordinate autophagosome maturation and endocytic trafficking.
AuthorsLiang C, Lee JS, Inn KS, Gack MU, Li Q, Roberts EA, Vergne I, Deretic V, Feng P, Akazawa C, Jung JU,
JournalNat Cell Biol
PubMed ID18552835
'Autophagic and endocytic pathways are tightly regulated membrane rearrangement processes that are crucial for homeostasis, development and disease. Autophagic cargo is delivered from autophagosomes to lysosomes for degradation through a complex process that topologically resembles endosomal maturation. Here, we report that a Beclin1-binding autophagic tumour suppressor, UVRAG, interacts with the ... More
Hip1-related mutant mice grow and develop normally but have accelerated spinal abnormalities and dwarfism in the absence of HIP1.
AuthorsHyun TS, Li L, Oravecz-Wilson KI, Bradley SV, Provot MM, Munaco AJ, Mizukami IF, Sun H, Ross TS,
JournalMol Cell Biol
PubMed ID15121852
In mice and humans, there are two known members of the Huntingtin interacting protein 1 (HIP1) family, HIP1 and HIP1-related (HIP1r). Based on structural and functional data, these proteins participate in the clathrin trafficking network. The inactivation of Hip1 in mice leads to spinal, hematopoietic, and testicular defects. To investigate ... More
Annexin A5 stimulates autophagy and inhibits endocytosis.
AuthorsGhislat G, Aguado C, Knecht E,
JournalJ Cell Sci
PubMed ID22266906
Macroautophagy is a major lysosomal catabolic process activated particularly under starvation in eukaryotic cells. A new organelle, the autophagosome, engulfs cytoplasmic substrates, which are degraded after fusion with endosomes and/or lysosomes. During a shotgun proteome analysis of purified lysosomal membranes from mouse fibroblasts, a Ca(2+)-dependent phospholipid-binding protein, annexin A5, was ... More
Automated organelle-based colocalization in whole-cell imaging.
AuthorsWoodcroft BJ, Hammond L, Stow JL, Hamilton NA,
JournalCytometry A
PubMed ID19746416
The use of fluorescence microscopy to investigate protein colocalization is an invaluable tool for understanding subcellular structures and their associated proteins. However, current techniques are largely limited to two-dimensional (2D) imaging and often require manual segmentation. Here, we present OBCOL, a methodology to automatically segment and quantify protein colocalization not ... More
Genetically encoded short peptide tags for orthogonal protein labeling by Sfp and AcpS phosphopantetheinyl transferases.
AuthorsZhou Z, Cironi P, Lin AJ, Xu Y, Hrvatin S, Golan DE, Silver PA, Walsh CT, Yin J
JournalACS Chem Biol
PubMed ID17465518
Short peptide tags S6 and A1, each 12 residues in length, were identified from a phage-displayed peptide library as efficient substrates for site-specific protein labeling catalyzed by Sfp and AcpS phosphopantetheinyl transferases (PPTases), respectively. S6 and A1 tags were selected for useful levels of orthogonality in reactivities with the PPTases: ... More
Interactions of GGA3 with the ubiquitin sorting machinery.
AuthorsPuertollano R, Bonifacino JS
JournalNat Cell Biol
PubMed ID15039775
The Golgi-localized, gamma-ear-containing, Arf-binding (GGA) proteins constitute a family of clathrin adaptors that are mainly associated with the trans-Golgi network (TGN) and mediate the sorting of mannose 6-phosphate receptors. This sorting is dependent on the interaction of the VHS domain of the GGAs with acidic-cluster-dileucine signals in the cytosolic tails ... More
Vacuolin-1 potently and reversibly inhibits autophagosome-lysosome fusion by activating RAB5A.
Authors
JournalAutophagy
PubMed ID25483964
Rab5 and Alsin regulate stress-activated cytoprotective signaling on mitochondria.
Authors
JournalElife
PubMed ID29469808
Molecularly Distinct Clathrin-Coated Pits Differentially Impact EGFR Fate and Signaling.
Authors
JournalCell Rep
PubMed ID31167147
Inhibition of EGF Uptake by Nephrotoxic Antisense Drugs In Vitro and Implications for Preclinical Safety Profiling.
Authors
JournalMol Ther Nucleic Acids
PubMed ID28325303
An endosomal tether undergoes an entropic collapse to bring vesicles together.
Authors
JournalNature
PubMed ID27556945
The IAP family member BRUCE regulates autophagosome-lysosome fusion.
Authors
JournalNat Commun
PubMed ID29426817
Endosomal escape of delivered mRNA from endosomal recycling tubules visualized at the nanoscale.