EnzChek™ Lysozyme Assay Kit - FAQs

Q: How was the substrate (Component A) in the EnzChek Lysozyme Assay Kit labeled?

The primary amines on the cell wall of Micrococcus lysodeikticus were conjugated to fluorescein. Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center .

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3 个常见问题解答

How can I limit signal saturation when using the EnzChek Lysozyme Assay Kit (Cat. No. E22013)?

To limit signal saturation when using the EnzChek Lysozyme Assay Kit (Cat. No. E22013), try lowering the voltage (gain) on the PMT (photomultiplier tube). If you are still seeing signal saturation after lowering the voltage, try a shorter reaction time, and/or lower substrate concentration.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How was the substrate (Component A) in the EnzChek Lysozyme Assay Kit labeled?

The primary amines on the cell wall of Micrococcus lysodeikticus were conjugated to fluorescein.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

May other buffers be used for the EnzChek Lysozyme assay?

Yes. You may use other buffers that are compatible with the enzyme of interest. The buffer provided is optimal for use with chicken egg white lysozyme. If the buffer has a pH <6, adjust the pH to 7.5 to 8 prior to detection for optimal signal from the fluorescein. The emission of fluorescein drops off at pH <6.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.