表皮生长因子、生物素化、与 Alexa Fluor™ 555 链霉亲和素(Alexa Fluor™ 555 EGF 复合物)的复合物
表皮生长因子、生物素化、与 Alexa Fluor™ 555 链霉亲和素(Alexa Fluor™ 555 EGF 复合物)的复合物
Invitrogen™

表皮生长因子、生物素化、与 Alexa Fluor™ 555 链霉亲和素(Alexa Fluor™ 555 EGF 复合物)的复合物

我们提供了几种表皮生长因子偶联物,这些偶联物对于检测细胞中的 EGF 受体非常有用。荧光标记的 EGF 使科学家能够考察受体-膜之间的相互作用,研究受体分布,计算 EGF 与受体相互作用的速率常数等。该偶联物含有荧光素 (FITC)了解更多信息
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货号数量
E35350
又称 E-35350
100 μg
货号 E35350
又称 E-35350
价格(CNY)
7,513.00
Each
添加至购物车
数量:
100 μg
价格(CNY)
7,513.00
Each
添加至购物车
我们提供了几种表皮生长因子偶联物,这些偶联物对于检测细胞中的 EGF 受体非常有用。荧光标记的 EGF 使科学家能够考察受体-膜之间的相互作用,研究受体分布,计算 EGF 与受体相互作用的速率常数等。该偶联物含有荧光素 (FITC)、Oregon Green™ 514 和四甲基罗丹明,使用与 EGF 直接连接的染料构建。EGF 与 Alexa Fluor™ 染料和 Texas Red™ 染料的偶联物是染料–链霉亲和素分子和生物素化 EGF 的复合物。生物素化偶联物利用生物素-XX,它含有一个长间隔臂,旨在增强探针对 EGF 受体的亲和力。

表皮生长因子偶联物规格:
• EGF 分子:53 个氨基酸,MW=6,045 Da
• 标记(激发/发射波长):生物素-XX 与 Alexa Fluor™ 555 链霉素亲和素 (∼555/565 nm) 的复合物
• 每个 EGF 分子上的荧光基团分子数量:∼2–3 个
• 通常使用流式细胞仪、荧光显微镜或荧光计监测荧光信号


查找更多的受体结合和吞噬作用探针
我们提供多种用于研究受体介导的内吞作用的荧光标记探针,用于内吞作用和胞吐作用的膜标记物,以及检测内吞的荧光配体的方法。查看 Molecular Probes™ 手册中用于追踪受体结合和吞噬作用的探针—第 16.1 节,了解有关这些产品的更多信息。

供研究使用。不可用于人或动物的治疗或诊断。
仅供科研使用。不可用于诊断程序。
规格
细胞类型哺乳动物细胞
检测方法荧光
标签或染料Alexa Fluor 染料
产品线Alexa Fluor
数量100 μg
运输条件湿冰
形式冻干
产品类型表皮生长因子
Unit SizeEach
内容与储存
储存在冰箱(-5 至 -30°C)中并避光。

引用和文献 (19)

引用和文献
Abstract
Regulation of endocytosis via the oxygen-sensing pathway.
Authors:Wang Y, Roche O, Yan MS, Finak G, Evans AJ, Metcalf JL, Hast BE, Hanna SC, Wondergem B, Furge KA, Irwin MS, Kim WY, Teh BT, Grinstein S, Park M, Marsden PA, Ohh M,
Journal:Nat Med
PubMed ID:19252501
'Tumor hypoxia is associated with disease progression, resistance to conventional cancer therapies and poor prognosis. Hypoxia, by largely unknown mechanisms, leads to deregulated accumulation of and signaling via receptor tyrosine kinases (RTKs) that are critical for driving oncogenesis. Here, we show that hypoxia or loss of von Hippel-Lindau protein--the principal ... More
Human ESCRT-II complex and its role in human immunodeficiency virus type 1 release.
Authors:Langelier C, von Schwedler UK, Fisher RD, De Domenico I, White PL, Hill CP, Kaplan J, Ward D, Sundquist WI
Journal:J Virol
PubMed ID:16973552
'The budding of many enveloped RNA viruses, including human immunodeficiency virus type 1 (HIV-1), requires some of the same cellular machinery as vesicle formation at the multivesicular body (MVB). In Saccharomyces cerevisiae, the ESCRT-II complex performs a central role in MVB protein sorting and vesicle formation, as it is recruited ... More
Two di-leucine motifs regulate trafficking of mucolipin-1 to lysosomes.
Authors:Vergarajauregui S, Puertollano R
Journal:Traffic
PubMed ID:16497227
'Mutations in the mucolipin-1 gene have been linked to mucolipidosis type IV, a lysosomal storage disorder characterized by severe neurological and ophthalmologic abnormalities. Mucolipin-1 is a membrane protein containing six putative transmembrane domains with both its N- and C-termini localized facing the cytosol. To gain information on the sorting motifs ... More
Rab14 is part of the early endosomal clathrin-coated TGN microdomain.
Authors:Proikas-Cezanne T, Gaugel A, Frickey T, Nordheim A,
Journal:FEBS Lett
PubMed ID:16962593
'Rab14 localizes to the Golgi/TGN and to early endosomes, but its biological function remains unclear. By structural modeling, we identified Rab14-specific residues and established a close relationship between the Rab2/Rab4/Rab14, Rab11/25 and Rab39 sub-groups within the Rab protein family. By quantitative confocal microscopy and by density centrifugation we show that ... More
Interactions of TOM1L1 with the multivesicular body sorting machinery.
Authors:Puertollano R
Journal:J Biol Chem
PubMed ID:15611048
'Tom1L1 (Tom1-like1) and related proteins Tom1 (Target of Myb1) and Tom1L2 (Tom1-like2) constitute a new protein family characterized by the presence of a VHS (Vps27p/Hrs/Stam) domain in the N-terminal portion followed by a GAT (GGA and Tom) domain. Recently it was demonstrated that the GAT domain of both Tom1 and ... More